##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL3_ACAGTG_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7195243	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.116049867947474	34.0	33.0	34.0	31.0	34.0
2	33.27844716293807	34.0	34.0	34.0	31.0	34.0
3	33.330905988859584	34.0	34.0	34.0	31.0	34.0
4	36.597994258150834	37.0	37.0	37.0	35.0	37.0
5	36.54110417118643	37.0	37.0	37.0	35.0	37.0
6	36.54176488549449	37.0	37.0	37.0	35.0	37.0
7	36.504709152977874	37.0	37.0	37.0	35.0	37.0
8	36.51503764362093	37.0	37.0	37.0	35.0	37.0
9	38.38918755071927	39.0	39.0	39.0	37.0	39.0
10-14	38.67188332624764	39.4	39.2	39.4	37.2	39.4
15-19	39.95347253734168	41.0	40.0	41.0	38.0	41.0
20-24	39.89355948089592	41.0	40.0	41.0	38.0	41.0
25-29	39.76837894147564	41.0	40.0	41.0	38.0	41.0
30-34	39.55195925974981	41.0	40.0	41.0	37.0	41.0
35-39	39.30781656714026	41.0	39.4	41.0	36.4	41.0
40-44	39.02394618222067	40.2	39.0	41.0	35.4	41.0
45-49	39.0010792408262	40.0	39.0	41.0	35.4	41.0
50-54	38.96042824404957	40.2	38.8	41.0	35.2	41.0
55-59	38.69561695136634	40.0	38.0	41.0	35.0	41.0
60-64	38.365578841465116	40.0	37.6	41.0	34.8	41.0
65-69	37.58411275338442	39.0	35.8	41.0	33.8	41.0
70-74	35.94997075150902	37.6	35.0	40.2	30.0	41.0
75-79	34.44958870742795	36.2	34.6	39.0	28.4	40.0
80-84	33.991275847111766	35.4	35.0	37.2	29.0	39.2
85-89	33.2777785267294	35.0	34.6	36.2	28.8	37.6
90-94	32.79123012245729	35.0	34.0	35.4	27.4	36.4
95-99	32.48391110626841	35.0	34.0	35.0	27.0	36.0
100-101	32.11382138171011	35.0	33.5	35.0	25.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	5.0
10	19.0
11	68.0
12	104.0
13	241.0
14	478.0
15	1091.0
16	1999.0
17	3431.0
18	4816.0
19	6126.0
20	7554.0
21	9184.0
22	11569.0
23	15064.0
24	19651.0
25	25894.0
26	34501.0
27	49434.0
28	80058.0
29	160244.0
30	197933.0
31	80453.0
32	69275.0
33	93116.0
34	142248.0
35	244562.0
36	519703.0
37	1357966.0
38	2712332.0
39	1336977.0
40	9141.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.975379949700017	28.905221044943787	27.9547205500712	19.164678455285006
2	19.76016654336761	34.13581723369176	27.98761626257793	18.1163999603627
3	19.85566510836166	27.323826917653975	33.89056971366094	18.92993826032343
4	19.870314191223194	27.227237577752835	27.87458257102338	25.027865660000586
5	26.27456779430521	27.616690638523256	27.663165788841322	18.445575778330213
6	26.12462400421835	27.75017383051292	27.19324908025834	18.931953085010385
7	20.061059786306036	33.854158921387366	27.492678148604572	18.592103143702026
8	19.68504746816751	33.795425672211486	27.519056687870027	19.000470171750976
9	25.441989936962518	27.506020297021237	27.913331071653868	19.138658694362373
10-14	20.609891841039975	30.063598963926587	27.789691050045146	21.536818144988292
15-19	20.613371624609147	28.643145891556486	29.06160347765786	21.681879006176505
20-24	20.74401686206141	29.76684523776268	28.81995673411052	20.669181166065393
25-29	20.91077732637459	28.521722713790844	28.614937080372048	21.952562879462526
30-34	19.798991589051944	29.701397640429345	28.496545744706932	22.003065025811782
35-39	22.284854732587007	28.414081554931542	28.488992254845147	20.812071457636304
40-44	19.92349769943568	28.376039330253388	29.65216450343398	22.048298466876954
45-49	22.33803310343432	28.30881046926561	29.58972524137284	19.76343118592723
50-54	21.075330943104913	27.131307006612897	28.426874667888068	23.366487382394123
55-59	21.096752320276536	27.10596507445707	30.793604580620716	21.003678024645676
60-64	21.05673086224284	28.43180688624585	29.540567631064267	20.970894620447037
65-69	19.807953008110783	33.32182923022484	27.040583373661626	19.82963438800275
70-74	20.00238979071177	32.85075648580331	27.09948159945946	20.04737212402547
75-79	20.257073849690254	31.982565018244703	27.303075675300292	20.45728545676475
80-84	20.565987118274712	31.43735391226387	27.293719397966264	20.702939571495154
85-89	20.844095874670103	31.122427209287128	27.167704228421236	20.86577268762153
90-94	21.1642648816567	30.980862490975465	26.861403652691752	20.993468974676087
95-99	21.498838979467234	31.04870960098321	26.384532135355993	21.067919284193565
100-101	21.798088137422166	31.199757387934206	25.88378595739515	21.118368517248477
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.14005840498502
2	37.87656650373032
3	38.78560336868509
4	44.89817985122378
5	44.720143572635415
6	45.05657708922873
7	38.65316293000806
8	38.68551763991849
9	44.58064863132489
10-14	42.14670998602827
15-19	42.29525063078565
20-24	41.4131980281268
25-29	42.86334020583711
30-34	41.80205661486372
35-39	43.096926190223314
40-44	41.97179616631263
45-49	42.10146428936155
50-54	44.441818325499035
55-59	42.10043034492221
60-64	42.02762548268988
65-69	39.637587396113524
70-74	40.04976191473724
75-79	40.71435930645501
80-84	41.26892668976986
85-89	41.70986856229163
90-94	42.15773385633278
95-99	42.566758263660795
100-101	42.91645665467065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	212.0
1	197.0
2	186.5
3	224.0
4	272.0
5	296.0
6	329.5
7	386.5
8	453.0
9	530.5
10	656.0
11	798.0
12	991.5
13	1270.5
14	1714.0
15	2443.5
16	3430.0
17	4949.5
18	7240.0
19	10578.5
20	15351.0
21	21724.5
22	29762.0
23	40091.5
24	52822.5
25	67096.5
26	82953.5
27	99935.0
28	117885.5
29	136494.5
30	154868.0
31	172667.5
32	189424.0
33	205955.5
34	222240.5
35	238593.0
36	254855.5
37	269839.0
38	282586.5
39	290584.5
40	292241.0
41	288121.5
42	278587.0
43	265202.0
44	250547.0
45	236547.5
46	223856.5
47	212943.0
48	203469.0
49	193933.0
50	182826.5
51	171273.0
52	160780.5
53	149743.0
54	139311.0
55	131268.0
56	123147.5
57	113530.0
58	102846.0
59	90225.5
60	76809.0
61	63817.5
62	52114.0
63	42361.5
64	34222.5
65	27408.5
66	21836.0
67	17345.5
68	13628.5
69	10665.5
70	8337.5
71	6494.5
72	5050.0
73	4002.0
74	3315.5
75	2744.0
76	2278.0
77	1945.0
78	1667.0
79	1401.5
80	1166.5
81	947.0
82	750.5
83	574.5
84	406.0
85	276.5
86	167.5
87	103.5
88	64.5
89	45.5
90	34.5
91	19.0
92	14.5
93	12.5
94	9.0
95	10.5
96	7.5
97	3.0
98	2.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13279607095966042
2	0.0
3	4.4473828055563935E-4
4	9.728649887154611E-5
5	0.0
6	2.2236914027781967E-4
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	1.334214841666918E-4
20-24	1.890137692361467E-4
25-29	2.7796142534727456E-6
30-34	2.5016528281254712E-5
35-39	1.2786225565974632E-4
40-44	1.1674379864585533E-4
45-49	3.585702386979842E-4
50-54	4.447382805556393E-5
55-59	0.0018178677217711757
60-64	0.0020430164763024683
65-69	0.0017928511934899212
70-74	0.01817311798920481
75-79	0.01604671308529816
80-84	0.00468642963135505
85-89	0.006370875868959534
90-94	0.00960634686000181
95-99	0.007479941956095158
100-101	0.003022830500651611
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7195243.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	11.20317324548006
#Duplication Level	Relative count
1	100.0
2	4.875596085550516
3	0.34931539475285933
4	0.15401633314103344
5	0.11326287041986652
6	0.08256545694158494
7	0.049750980464801184
8	0.03493153947528593
9	0.028051084730153858
10++	0.16566017963279542
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC	400945	5.5723621842931506	TruSeq Adapter, Index 5 (100% over 50bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	9221355	5.562278	25.595173	65-69
CACAC	2113175	3.6893888	16.536957	30-34
CTGGG	1311070	3.4488678	3.7018197	1
CCCAG	1354650	3.369331	3.8836675	25-29
TTTTT	4147135	3.3222866	3.8904967	15-19
GGAAG	1840365	3.2096288	75.78967	5
CCTCC	1169910	3.080855	3.5426738	65-69
CCTGG	1159160	3.050353	3.2909825	65-69
GGAGG	1223000	3.0386095	3.3797126	4
CTCCA	1477950	2.7310202	17.521141	20-24
CAGTG	1474350	2.722411	17.481146	35-39
TCCAG	1450160	2.678706	17.203089	25-29
CTGCT	1337880	2.6156068	18.43888	55-59
CACAG	1470190	2.56588	16.826908	30-34
TCTGC	1283750	2.5097806	18.289454	55-59
AGAGC	1428885	2.4928958	74.88423	8
GAGCA	1376160	2.4009094	74.72939	9
TTCTG	1650410	2.3962917	13.908242	55-59
GCACA	1359360	2.3724518	15.973051	10-14
AGCAC	1353115	2.3615525	15.862683	10-14
GAAGA	1900155	2.3261743	53.175755	6
CGTCT	1178045	2.3031232	16.86976	50-54
CTTCT	1564840	2.2728658	14.08051	50-54
AAGAG	1854720	2.2705526	53.10785	7
GTCTG	1150295	2.2480633	17.741152	15-19
ACTCC	1201120	2.2194817	17.15015	20-24
ACACA	1802940	2.2087498	11.962922	30-34
TCTTC	1443430	2.0965233	13.968934	50-54
CCAGT	1129480	2.086352	16.822737	25-29
GATCG	1122380	2.0724926	78.83819	1
TGCTT	1396940	2.0282693	13.984995	55-59
TCTGA	1447055	1.9851197	13.051907	15-19
GCTTG	1010485	1.9748274	17.929356	55-59
ATGCC	1062995	1.9635426	16.837372	45-49
CTGAA	1498825	1.9427027	12.321903	15-19
ATCGG	1040405	1.9211245	78.74357	2
GAAAA	2227475	1.9141166	8.75548	60-64
TCGGA	1008845	1.8628483	78.665665	3
AGTGA	1424420	1.8455995	12.147492	35-39
CAGTC	998600	1.8445935	16.63265	25-29
TCACA	1399345	1.8144137	12.065857	30-34
CGGAA	1002745	1.7494332	74.340614	4
CTTGA	1275195	1.7493563	12.672821	60-64
ATCTC	1274720	1.749333	12.421955	40-44
GTCAC	932010	1.7215899	16.743193	25-29
GTCTT	1175375	1.7065707	13.436787	50-54
TGAAA	1875810	1.7060454	8.940398	60-64
GTGAT	1163795	1.5959601	12.532661	35-39
AACTC	1222730	1.5854117	11.910497	20-24
TGAAC	1218815	1.5797677	11.764915	20-24
AGATC	1212990	1.5722177	6.64527	95-97
ACAGT	1203705	1.5601829	12.023053	30-34
TTGAA	1594385	1.5347625	9.265241	60-64
GAACT	1175650	1.5238194	11.831293	20-24
CCGTC	574140	1.511404	22.242311	50-54
AGTCA	1144335	1.4832305	12.067745	25-29
TGCCG	557170	1.4662043	23.05722	45-49
CACGT	786720	1.453213	16.162943	10-14
TGATC	1045025	1.4336011	12.388545	35-39
GCCGT	534410	1.4063107	22.73435	45-49
GATCT	1024785	1.4058352	12.313768	35-39
ACACG	795180	1.3878046	15.298661	10-14
TCTCG	645105	1.2612052	16.682608	40-44
ACGTC	682410	1.2605338	15.743968	15-19
TATGC	893045	1.2251097	12.258097	45-49
GTATG	889175	1.2193625	12.076926	45-49
CTCGT	560720	1.0962292	16.57642	40-44
CGTAT	543080	0.7450157	11.864116	40-44
TCGTA	518535	0.7113441	11.950204	40-44
>>END_MODULE