FastQCFastQC Report
Mon 21 Jul 2014
ZL3_ACAGTG_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL3_ACAGTG_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7569902
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 350560 4.6309714445444605 TruSeq Adapter, Index 5 (100% over 50bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 9595090 5.545954 25.236393 65-69
CACAC 2112150 3.535154 16.084497 30-34
CTGGG 1375710 3.4542356 3.754671 1
CCCAG 1422475 3.3878257 3.9245925 25-29
TTTTT 4314135 3.3023522 3.842875 15-19
GGAAG 1919805 3.1971486 73.42201 5
CCTCC 1221310 3.0819728 3.5783658 65-69
CCTGG 1213825 3.052861 3.3213785 70-74
GGAGG 1280280 3.0339155 3.5129707 4
CCAGG 1262740 3.0023715 3.4291997 25-29
CTCCA 1535160 2.7179265 17.09448 20-24
CAGTG 1529875 2.6995296 16.979504 35-39
TCCAG 1507250 2.6640565 16.739096 25-29
CTGCT 1384515 2.5885484 17.886635 55-59
CACAG 1527460 2.5522752 16.420498 30-34
TCTGC 1334150 2.494384 17.843924 55-59
AGAGC 1489515 2.4847147 72.6573 8
TTCTG 1719855 2.3863387 13.576778 55-59
GAAGA 1983605 2.3214965 51.57212 6
GAGCA 1369720 2.2848804 70.697914 9
AAGAG 1942405 2.2732785 51.558025 7
CGTCT 1211495 2.2650628 16.380444 15-19
CTTCT 1627500 2.2619722 13.719316 50-54
GCACA 1347450 2.2514913 14.7907715 10-14
AGCAC 1343045 2.244131 14.673268 10-14
GTCTG 1195555 2.2315273 17.233383 15-19
ACTCC 1246265 2.206452 16.740723 20-24
ACACA 1861845 2.186293 11.629201 30-34
TCTTC 1499120 2.0835443 13.663856 50-54
CCAGT 1169715 2.067465 16.338396 25-29
GATCG 1170695 2.065741 76.44713 1
TGCTT 1445980 2.006331 13.598981 55-59
TCTGA 1506360 1.9759165 12.698093 15-19
GCTTG 1048210 1.956505 17.391693 55-59
ATGCC 1099335 1.9430686 16.389793 45-49
CTGAA 1565805 1.9416771 12.0049305 15-19
ATCGG 1084290 1.913276 76.329956 2
GAAAA 2324490 1.9118232 8.564081 60-64
TCGGA 1050685 1.8539784 76.306145 3
CAGTC 1037750 1.8342175 16.180977 25-29
AGTGA 1475570 1.8267251 11.828703 35-39
TCACA 1451725 1.803224 11.760478 30-34
CGGAA 1045545 1.7441121 72.11143 4
CTTGA 1328520 1.742641 12.382197 60-64
ATCTC 1326300 1.7426394 12.112216 40-44
TGAAA 1957585 1.703104 8.730381 60-64
GTCAC 959270 1.6955045 16.231236 25-29
GTCTT 1220950 1.6940967 13.044549 50-54
AGATC 1275995 1.5822979 6.673619 95-97
GTGAT 1203440 1.575935 12.162173 35-39
AACTC 1268665 1.5758404 11.640543 20-24
TGAAC 1264915 1.5685582 11.446237 20-24
ACAGT 1249525 1.5494739 11.720994 30-34
TTGAA 1654610 1.5227079 9.03508 60-64
GAACT 1220725 1.5137602 11.506356 20-24
CCGTC 589360 1.4847646 21.631945 50-54
AGTCA 1190570 1.4763665 11.735077 25-29
TGCCG 571255 1.4367491 22.383108 45-49
CACGT 812025 1.4352498 17.292908 10-14
TGATC 1084400 1.4224249 12.052422 35-39
GATCT 1059545 1.389822 11.94141 35-39
GCCGT 547810 1.3777832 22.0328 45-49
ACACG 757815 1.2662541 14.089312 10-14
TCTCG 665050 1.2434058 16.208353 40-44
ACGTC 702620 1.2418771 15.294006 15-19
TATGC 922065 1.2094874 11.914961 45-49
GTATG 918515 1.2028185 11.706606 45-49
CTCGT 576480 1.0778116 16.107975 40-44
CGTAT 557330 0.73105866 11.524421 40-44
TCGTA 532930 0.6990528 11.607236 40-44
Produced by FastQC (version 0.10.1)