##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL3_ACAGTG_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7569902	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.15842833896661	34.0	33.0	34.0	31.0	34.0
2	33.29476365744233	34.0	34.0	34.0	31.0	34.0
3	33.32868325111738	34.0	34.0	34.0	31.0	34.0
4	36.598810922519206	37.0	37.0	37.0	35.0	37.0
5	36.54466583583248	37.0	37.0	37.0	35.0	37.0
6	36.53590878719434	37.0	37.0	37.0	35.0	37.0
7	36.506195060385195	37.0	37.0	37.0	35.0	37.0
8	36.517484374302335	37.0	37.0	37.0	35.0	37.0
9	38.38529336311091	39.0	39.0	39.0	37.0	39.0
10-14	36.797370216946014	39.4	38.4	39.4	26.2	39.4
15-19	39.44856414257411	41.0	39.0	41.0	36.6	41.0
20-24	39.89259897948481	41.0	40.0	41.0	38.0	41.0
25-29	39.76905360201493	41.0	40.0	41.0	38.0	41.0
30-34	39.562177370327916	41.0	40.0	41.0	37.0	41.0
35-39	39.32551428010561	41.0	39.6	41.0	36.4	41.0
40-44	39.04793924676964	40.0	39.0	41.0	35.4	41.0
45-49	39.01672156389871	40.0	39.0	41.0	35.6	41.0
50-54	38.96631412137172	40.0	38.8	41.0	35.2	41.0
55-59	38.698330308635434	40.0	38.2	41.0	35.0	41.0
60-64	38.37031182173824	40.0	37.4	41.0	34.8	41.0
65-69	37.594012815489556	39.0	35.8	41.0	33.8	41.0
70-74	35.99947708702173	37.4	35.0	40.0	30.4	41.0
75-79	34.468033535969155	36.0	34.6	39.0	28.6	40.0
80-84	33.99796652585463	35.2	35.0	37.2	29.0	39.0
85-89	33.30512598445792	35.0	34.6	36.2	29.0	37.4
90-94	32.82686454857672	35.0	34.0	35.4	28.4	36.4
95-99	32.54689326229059	35.0	34.0	35.0	27.0	36.0
100-101	32.19455139577765	35.0	33.5	35.0	26.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	0.0
8	0.0
9	7.0
10	14.0
11	38.0
12	97.0
13	170.0
14	435.0
15	1003.0
16	2073.0
17	3532.0
18	4879.0
19	6271.0
20	7428.0
21	9274.0
22	11575.0
23	15338.0
24	20295.0
25	26566.0
26	36246.0
27	52511.0
28	88325.0
29	166969.0
30	194812.0
31	85016.0
32	76067.0
33	104133.0
34	163474.0
35	292347.0
36	656348.0
37	1658553.0
38	2712683.0
39	1165712.0
40	7709.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.81406811120286	28.951394546040603	28.046810199710627	19.187727143045908
2	19.816016112229722	34.002078230339045	28.034325939754567	18.147579717676663
3	19.877879528691388	27.4081883754902	33.7333825457714	18.980549550047016
4	19.90058788079423	27.257473082214272	27.958354018321508	24.88358501866999
5	26.14525260696902	27.652682954151853	27.709592541620747	18.492471897258376
6	25.993229502839004	27.817850217876007	27.20792158207596	18.980998697209024
7	20.093047439715864	33.741599296794064	27.54782030203297	18.617532961457098
8	19.72561864076972	33.683989568160854	27.58121571454954	19.009176076519882
9	25.332150931412322	27.575839158816056	27.973506129934044	19.118503779837575
10-14	20.77013903949493	29.996260908953808	27.75061589408815	21.482984157463115
15-19	20.62356961343	28.64946778252198	29.086065694286717	21.640896909761302
20-24	20.742002348146823	29.75063133064489	28.84223436754199	20.665131953666293
25-29	20.913227146137427	28.558010394322146	28.63241558477243	21.896346874767996
30-34	19.842243310169177	29.69183547702508	28.506945614101138	21.9589755987046
35-39	22.243512130073466	28.448037052911822	28.51161527061919	20.79683554639552
40-44	19.953544360139954	28.39482886946732	29.64309565364519	22.008531116747537
45-49	22.292275978863287	28.333358300948753	29.58693603473949	19.78742968544847
50-54	21.06646756200356	27.185757569993108	28.456101332014423	23.291673535988906
55-59	21.073291706618853	27.163853958946575	30.75988604525745	21.002968289177122
60-64	21.026753054398856	28.457660350160413	29.528538150163637	20.987048445277097
65-69	19.832828679119977	33.20795581145158	27.095096172996463	19.86411933643198
70-74	20.019689082777784	32.78186566452861	27.139884316200625	20.058560936492984
75-79	20.263662932169353	31.912613508026972	27.36036698961187	20.463356570191806
80-84	20.577090010352972	31.33261125415747	27.362734611331568	20.727564124157986
85-89	20.873023728391164	31.01477431518958	27.22019648743736	20.892005468981896
90-94	21.174940876170172	30.90520198544565	26.91090556397482	21.00895157440936
95-99	21.5053636730609	31.013173884698798	26.430466502137328	21.05099594010298
100-101	21.7703179880577	31.15886112814204	25.96384387416919	21.106977009631073
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.00179525424876
2	37.96359582990638
3	38.8584290787384
4	44.78417289946422
5	44.6377245042274
6	44.97422820004803
7	38.71058040117296
8	38.7347947172896
9	44.450654711249896
10-14	42.25312319695804
15-19	42.2644665231913
20-24	41.407134301813116
25-29	42.80957402090542
30-34	41.801218908873786
35-39	43.04034767646899
40-44	41.96207547688749
45-49	42.07970566431175
50-54	44.358141097992466
55-59	42.07625999579597
60-64	42.01380149967595
65-69	39.69694801555196
70-74	40.07825001927077
75-79	40.72701950236116
80-84	41.30465413451096
85-89	41.76502919737306
90-94	42.183892450579535
95-99	42.55635961316388
100-101	42.87729499768877
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	227.0
1	205.0
2	185.0
3	238.0
4	290.0
5	301.0
6	357.0
7	419.5
8	487.0
9	553.0
10	655.5
11	839.0
12	1065.5
13	1379.0
14	1861.5
15	2600.5
16	3671.5
17	5261.5
18	7780.5
19	11320.5
20	16216.5
21	23054.0
22	31684.5
23	42443.5
24	55548.0
25	70685.0
26	87684.0
27	106000.0
28	125213.0
29	145087.0
30	164556.5
31	183305.5
32	201454.5
33	218587.5
34	236134.5
35	252762.0
36	268583.0
37	283878.0
38	296096.0
39	304287.5
40	306331.5
41	302097.5
42	292670.0
43	279175.0
44	264237.0
45	249405.0
46	236069.5
47	224364.5
48	213313.0
49	202640.0
50	191480.0
51	179788.0
52	167691.0
53	156197.5
54	146114.0
55	137528.5
56	129270.0
57	119321.5
58	107210.5
59	94200.5
60	80531.5
61	66481.0
62	54250.0
63	44036.5
64	35398.5
65	28365.0
66	22689.0
67	17965.0
68	14027.5
69	10972.0
70	8572.5
71	6652.5
72	5256.0
73	4273.5
74	3438.5
75	2807.5
76	2395.0
77	2079.0
78	1778.0
79	1459.0
80	1184.5
81	958.0
82	748.5
83	556.5
84	392.0
85	255.5
86	153.5
87	85.5
88	57.5
89	35.5
90	22.5
91	19.0
92	12.5
93	9.5
94	7.5
95	7.5
96	6.0
97	5.0
98	3.5
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12434771282375916
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	2.4185253653217704
15-19	1.0303964304954014E-4
20-24	2.3249970739383415E-4
25-29	0.0
30-34	2.24573581005408E-4
35-39	1.823009069338018E-4
40-44	1.823009069338018E-4
45-49	0.0012708222642776616
50-54	1.849429490632772E-4
55-59	2.5892012868858805E-4
60-64	0.0
65-69	0.0030436325331556473
70-74	0.019072902132682826
75-79	0.015960576504160818
80-84	0.0012549700115008094
85-89	0.006901014042189714
90-94	0.008177120390726326
95-99	0.00462885781084088
100-101	0.004504681830755536
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7569902.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	10.606667074784445
#Duplication Level	Relative count
1	100.0
2	4.44021850784637
3	0.32502251979371344
4	0.1611943128960718
5	0.09956119325933847
6	0.08639172325148947
7	0.037928073622605134
8	0.03371384322009345
9	0.020017594411930486
10++	0.15171229449042054
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC	350560	4.6309714445444605	TruSeq Adapter, Index 5 (100% over 50bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	9595090	5.545954	25.236393	65-69
CACAC	2112150	3.535154	16.084497	30-34
CTGGG	1375710	3.4542356	3.754671	1
CCCAG	1422475	3.3878257	3.9245925	25-29
TTTTT	4314135	3.3023522	3.842875	15-19
GGAAG	1919805	3.1971486	73.42201	5
CCTCC	1221310	3.0819728	3.5783658	65-69
CCTGG	1213825	3.052861	3.3213785	70-74
GGAGG	1280280	3.0339155	3.5129707	4
CCAGG	1262740	3.0023715	3.4291997	25-29
CTCCA	1535160	2.7179265	17.09448	20-24
CAGTG	1529875	2.6995296	16.979504	35-39
TCCAG	1507250	2.6640565	16.739096	25-29
CTGCT	1384515	2.5885484	17.886635	55-59
CACAG	1527460	2.5522752	16.420498	30-34
TCTGC	1334150	2.494384	17.843924	55-59
AGAGC	1489515	2.4847147	72.6573	8
TTCTG	1719855	2.3863387	13.576778	55-59
GAAGA	1983605	2.3214965	51.57212	6
GAGCA	1369720	2.2848804	70.697914	9
AAGAG	1942405	2.2732785	51.558025	7
CGTCT	1211495	2.2650628	16.380444	15-19
CTTCT	1627500	2.2619722	13.719316	50-54
GCACA	1347450	2.2514913	14.7907715	10-14
AGCAC	1343045	2.244131	14.673268	10-14
GTCTG	1195555	2.2315273	17.233383	15-19
ACTCC	1246265	2.206452	16.740723	20-24
ACACA	1861845	2.186293	11.629201	30-34
TCTTC	1499120	2.0835443	13.663856	50-54
CCAGT	1169715	2.067465	16.338396	25-29
GATCG	1170695	2.065741	76.44713	1
TGCTT	1445980	2.006331	13.598981	55-59
TCTGA	1506360	1.9759165	12.698093	15-19
GCTTG	1048210	1.956505	17.391693	55-59
ATGCC	1099335	1.9430686	16.389793	45-49
CTGAA	1565805	1.9416771	12.0049305	15-19
ATCGG	1084290	1.913276	76.329956	2
GAAAA	2324490	1.9118232	8.564081	60-64
TCGGA	1050685	1.8539784	76.306145	3
CAGTC	1037750	1.8342175	16.180977	25-29
AGTGA	1475570	1.8267251	11.828703	35-39
TCACA	1451725	1.803224	11.760478	30-34
CGGAA	1045545	1.7441121	72.11143	4
CTTGA	1328520	1.742641	12.382197	60-64
ATCTC	1326300	1.7426394	12.112216	40-44
TGAAA	1957585	1.703104	8.730381	60-64
GTCAC	959270	1.6955045	16.231236	25-29
GTCTT	1220950	1.6940967	13.044549	50-54
AGATC	1275995	1.5822979	6.673619	95-97
GTGAT	1203440	1.575935	12.162173	35-39
AACTC	1268665	1.5758404	11.640543	20-24
TGAAC	1264915	1.5685582	11.446237	20-24
ACAGT	1249525	1.5494739	11.720994	30-34
TTGAA	1654610	1.5227079	9.03508	60-64
GAACT	1220725	1.5137602	11.506356	20-24
CCGTC	589360	1.4847646	21.631945	50-54
AGTCA	1190570	1.4763665	11.735077	25-29
TGCCG	571255	1.4367491	22.383108	45-49
CACGT	812025	1.4352498	17.292908	10-14
TGATC	1084400	1.4224249	12.052422	35-39
GATCT	1059545	1.389822	11.94141	35-39
GCCGT	547810	1.3777832	22.0328	45-49
ACACG	757815	1.2662541	14.089312	10-14
TCTCG	665050	1.2434058	16.208353	40-44
ACGTC	702620	1.2418771	15.294006	15-19
TATGC	922065	1.2094874	11.914961	45-49
GTATG	918515	1.2028185	11.706606	45-49
CTCGT	576480	1.0778116	16.107975	40-44
CGTAT	557330	0.73105866	11.524421	40-44
TCGTA	532930	0.6990528	11.607236	40-44
>>END_MODULE