FastQCFastQC Report
Mon 21 Jul 2014
ZL2_TGACCA_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL2_TGACCA_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11193634
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC 32852 0.2934882451936521 TruSeq Adapter, Index 4 (100% over 50bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 8209875 3.6412446 4.6408796 65-69
TTTTT 7719200 3.470884 4.1076446 15-19
CCCAG 2081615 3.4463112 3.965414 25-29
CTGGG 2003830 3.2613993 3.5065513 1
GGAGG 2023650 3.2522707 3.6093037 4
CCTCC 1925065 3.2276862 3.722181 70-74
CCAGG 1868475 3.0629532 3.4654157 25-29
GGGGG 1356830 3.0085773 3.507695 70-74
GGAAG 1799145 2.0957184 6.24654 5
GAGCA 1185110 1.3942043 5.4666204 9
AGAGC 1176970 1.384628 5.4569216 8
Produced by FastQC (version 0.10.1)