##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL2_TGACCA_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	11193634	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.19116588946896	34.0	33.0	34.0	31.0	34.0
2	33.36140041741583	34.0	34.0	34.0	31.0	34.0
3	33.4030510556268	34.0	34.0	34.0	31.0	34.0
4	36.647224752926526	37.0	37.0	37.0	35.0	37.0
5	36.58863100222859	37.0	37.0	37.0	35.0	37.0
6	36.59165691856639	37.0	37.0	37.0	35.0	37.0
7	36.58806416218361	37.0	37.0	37.0	35.0	37.0
8	36.58142029657214	37.0	37.0	37.0	35.0	37.0
9	38.45437871204293	39.0	39.0	39.0	37.0	39.0
10-14	38.759485382495086	39.4	39.2	39.4	37.2	39.4
15-19	40.04359363545386	41.0	40.0	41.0	38.0	41.0
20-24	39.97288206850429	41.0	40.0	41.0	38.0	41.0
25-29	39.82398572259911	41.0	40.0	41.0	38.0	41.0
30-34	39.64201293342269	41.0	40.0	41.0	37.4	41.0
35-39	39.4460478875761	41.0	40.0	41.0	37.0	41.0
40-44	39.21523853647529	41.0	39.0	41.0	36.0	41.0
45-49	39.03868611391082	40.0	39.0	41.0	35.0	41.0
50-54	38.93867248116206	40.0	38.8	41.0	35.0	41.0
55-59	38.59956836180279	40.0	38.0	41.0	35.0	41.0
60-64	38.30027063597041	40.0	37.0	41.0	34.8	41.0
65-69	37.68826277507377	39.2	35.6	41.0	34.0	41.0
70-74	36.89296580538546	37.6	35.0	40.2	33.6	41.0
75-79	35.73205977611918	36.2	34.8	39.0	32.4	40.0
80-84	35.34943324035787	35.4	35.0	37.2	33.0	39.2
85-89	34.66474803446316	35.0	35.0	36.2	33.0	37.6
90-94	34.18693827223581	35.0	35.0	35.6	32.4	36.6
95-99	33.88602216223971	35.0	34.6	35.0	32.0	36.0
100-101	33.512474947814084	35.0	34.0	35.0	31.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	10.0
12	31.0
13	109.0
14	413.0
15	1230.0
16	2917.0
17	5490.0
18	7747.0
19	9710.0
20	11242.0
21	12925.0
22	15012.0
23	17360.0
24	20816.0
25	25021.0
26	30360.0
27	37372.0
28	47151.0
29	63128.0
30	79804.0
31	87376.0
32	112087.0
33	154856.0
34	245383.0
35	432294.0
36	941867.0
37	2322086.0
38	4337093.0
39	2155940.0
40	16798.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.626434331880198	30.60516006577086	29.50463157486069	20.263774027488253
2	20.850065313909674	30.30514487073635	29.685390821247147	19.15939899410683
3	20.880936478991366	29.170030199432034	29.830846016487296	20.118187305089307
4	21.08747005212079	28.904150169909375	29.612384347003566	20.395995430966263
5	21.729109599259722	29.282626178415338	29.387006936263955	19.60125728606099
6	21.454754112683823	29.537147243832035	28.88749940479397	20.120599238690172
7	21.285437776507614	29.819824375176108	29.215561273488127	19.679176574828155
8	20.71673059883859	29.849242882159626	29.30447788448327	20.129548634518514
9	20.83356486374309	29.318584116650587	29.692519873349443	20.15533114625688
10-14	20.574290708450892	29.52567682666773	29.599182892704906	20.30084957217647
15-19	20.595317743082017	29.237686221051472	29.637896376498073	20.52909965936843
20-24	20.7727038210431	29.147003966618968	29.425782573020033	20.654509639317904
25-29	20.958230740047973	29.050875328796266	29.20578123611655	20.78511269503921
30-34	21.014060105836638	28.99057288698274	29.144228157511463	20.851138849669155
35-39	21.13495290585341	28.96992111685331	28.957749898326014	20.937376078967265
40-44	21.139076654011664	28.867987188635475	29.028187313988973	20.964748843363886
45-49	21.285438681674655	28.80800672170112	28.968812968487782	20.937741628136447
50-54	21.184901560875442	28.75865650929315	28.908196936246394	21.14824499358501
55-59	21.231068214018094	28.741932003716897	28.976682344086395	21.050317438178613
60-64	21.157144772816146	28.809073649699524	28.911857625833985	21.121923951650345
65-69	21.125899622180796	29.05628640466401	28.728339227703632	21.089474745451557
70-74	21.179261584685896	29.036397306440026	28.702539233130747	21.08180187574333
75-79	21.17116370112765	28.99247502396744	28.741751497446856	21.094609777458054
80-84	21.191113633940873	28.995914509568006	28.73307520467924	21.079896651811886
85-89	21.164480565962013	29.015198650279515	28.699664678371057	21.12065610538741
90-94	21.1819905523397	29.020569461721163	28.633881443347697	21.163558542591442
95-99	21.261843378643608	29.13594261715081	28.467038311936154	21.135175692269424
100-101	21.37556157077801	29.17608918252966	28.30069250996704	21.147656736725292
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.89020835936845
2	40.009464308016504
3	40.99912378408067
4	41.48346548308705
5	41.33036688532071
6	41.575353351374
7	40.96461435133577
8	40.8462792333571
9	40.98889600999997
10-14	40.87514028062736
15-19	41.12441740245045
20-24	41.42721346036101
25-29	41.74334343508718
30-34	41.8651989555058
35-39	42.07232898482067
40-44	42.10382549737555
45-49	42.223180309811106
50-54	42.333146554460455
55-59	42.281385652196704
60-64	42.27906872446649
65-69	42.21537436763235
70-74	42.26106346042923
75-79	42.2657734785857
80-84	42.27101028575276
85-89	42.28513667134942
90-94	42.34554909493114
95-99	42.39701907091303
100-101	42.5232183075033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	358.0
1	325.0
2	334.5
3	448.0
4	551.5
5	578.5
6	604.0
7	732.0
8	923.5
9	1181.0
10	1633.0
11	2212.5
12	2970.0
13	3929.5
14	5268.0
15	7147.0
16	9430.0
17	12603.5
18	16952.0
19	22881.0
20	30887.5
21	41360.0
22	54585.5
23	71151.0
24	91218.0
25	114490.0
26	140134.0
27	168018.5
28	197174.5
29	226239.5
30	255008.0
31	282555.5
32	309115.5
33	333434.0
34	354657.5
35	372517.5
36	387334.0
37	398713.0
38	404755.5
39	405432.5
40	401172.0
41	392772.5
42	380577.0
43	367318.0
44	354555.5
45	342917.0
46	332799.0
47	324253.0
48	317057.0
49	308268.5
50	295867.0
51	280297.5
52	263218.5
53	246406.0
54	231053.0
55	216903.0
56	202910.5
57	187429.0
58	170177.0
59	150822.5
60	129602.5
61	108627.5
62	90315.0
63	74457.5
64	60732.5
65	49302.5
66	39522.5
67	31584.5
68	24956.5
69	19297.5
70	14950.0
71	11530.0
72	8912.0
73	6974.5
74	5470.0
75	4426.5
76	3603.0
77	2980.0
78	2450.5
79	1965.5
80	1547.5
81	1199.5
82	917.5
83	658.0
84	433.5
85	272.0
86	159.5
87	103.0
88	65.5
89	44.0
90	34.5
91	28.5
92	21.0
93	13.0
94	10.0
95	8.0
96	6.0
97	5.0
98	4.5
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13242348284748276
2	0.0
3	4.1094786554572E-4
4	1.7867298501987827E-4
5	0.0
6	2.769431267808113E-4
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	1.340047387649087E-4
20-24	1.8224644472027583E-4
25-29	1.7867298501987826E-6
30-34	5.002843580556592E-5
35-39	1.2149762981351722E-4
40-44	8.754976265974036E-5
45-49	3.484123207887626E-4
50-54	4.824170595536713E-5
55-59	0.0018796398024091192
60-64	0.0022780805590034477
65-69	0.0016527251114338741
70-74	0.01726159708277044
75-79	0.01594120372347354
80-84	0.004995696661155796
85-89	0.007129052102293143
90-94	0.010261189529691609
95-99	0.007879478639376632
100-101	0.0032652488012382755
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.1193634E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.412957825053923
#Duplication Level	Relative count
1	100.0
2	4.329829587344385
3	0.3191287679312451
4	0.1648305352516167
5	0.12006824932066648
6	0.06740673646072505
7	0.06530027594632738
8	0.05424135824573968
9	0.03791628925915784
10++	0.16430392012301728
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC	32852	0.2934882451936521	TruSeq Adapter, Index 4 (100% over 50bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	8209875	3.6412446	4.6408796	65-69
TTTTT	7719200	3.470884	4.1076446	15-19
CCCAG	2081615	3.4463112	3.965414	25-29
CTGGG	2003830	3.2613993	3.5065513	1
GGAGG	2023650	3.2522707	3.6093037	4
CCTCC	1925065	3.2276862	3.722181	70-74
CCAGG	1868475	3.0629532	3.4654157	25-29
GGGGG	1356830	3.0085773	3.507695	70-74
GGAAG	1799145	2.0957184	6.24654	5
GAGCA	1185110	1.3942043	5.4666204	9
AGAGC	1176970	1.384628	5.4569216	8
>>END_MODULE