FastQCFastQC Report
Mon 21 Jul 2014
ZL2_TGACCA_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL2_TGACCA_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11447782
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC 28239 0.246676605127526 TruSeq Adapter, Index 4 (100% over 50bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 8310655 3.6216133 4.5918503 65-69
TTTTT 7863115 3.4696789 4.135772 15-19
CCCAG 2114275 3.451042 3.9443674 25-29
CTGGG 2034810 3.26339 3.503137 80-84
GGAGG 2046050 3.2404935 3.587519 4
CCTCC 1948070 3.2199082 3.7525291 65-69
CCAGG 1891995 3.0573428 3.4027522 25-29
GGGGG 1378510 3.014476 3.5231748 70-74
GGAAG 1829395 2.098434 6.130299 5
GAGCA 1203830 1.3948183 5.2769976 9
AGAGC 1201910 1.3925937 5.357962 8
Produced by FastQC (version 0.10.1)