##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL2_TGACCA_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	11447782	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.22744807684143	34.0	33.0	34.0	31.0	34.0
2	33.372647295345075	34.0	34.0	34.0	31.0	34.0
3	33.397895155585594	34.0	34.0	34.0	31.0	34.0
4	36.64525940483493	37.0	37.0	37.0	35.0	37.0
5	36.58920374269881	37.0	37.0	37.0	35.0	37.0
6	36.58085950623448	37.0	37.0	37.0	35.0	37.0
7	36.583523341027984	37.0	37.0	37.0	35.0	37.0
8	36.57849171132015	37.0	37.0	37.0	35.0	37.0
9	38.4456731443698	39.0	39.0	39.0	37.0	39.0
10-14	36.878754609408176	39.4	38.4	39.4	26.2	39.4
15-19	39.537410408409244	41.0	39.0	41.0	36.6	41.0
20-24	39.968396620410836	41.0	40.0	41.0	38.0	41.0
25-29	39.8192384341351	41.0	40.0	41.0	38.0	41.0
30-34	39.6435305459171	41.0	40.0	41.0	37.4	41.0
35-39	39.45587185360448	41.0	40.0	41.0	37.0	41.0
40-44	39.22705224470557	41.0	39.0	41.0	36.0	41.0
45-49	39.047501760603055	40.0	39.0	41.0	35.0	41.0
50-54	38.94090284039301	40.0	38.8	41.0	35.0	41.0
55-59	38.59661433105557	40.0	38.0	41.0	35.0	41.0
60-64	38.29214069590074	40.0	37.0	41.0	34.8	41.0
65-69	37.678810672670046	39.0	35.6	41.0	34.0	41.0
70-74	36.86124976873249	37.6	35.0	40.0	33.6	41.0
75-79	35.70126366836824	36.2	34.8	39.0	32.4	40.0
80-84	35.310310014638645	35.4	35.0	37.2	33.0	39.0
85-89	34.646213126700005	35.0	35.0	36.2	33.0	37.4
90-94	34.17546090587679	35.0	35.0	35.6	32.4	36.4
95-99	33.89915435147175	35.0	34.6	35.0	32.0	36.0
100-101	33.54317487003159	35.0	34.0	35.0	31.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	7.0
12	21.0
13	95.0
14	387.0
15	1261.0
16	2917.0
17	5620.0
18	8094.0
19	9913.0
20	11187.0
21	12857.0
22	14740.0
23	17572.0
24	20591.0
25	24803.0
26	30597.0
27	38051.0
28	49115.0
29	65399.0
30	82489.0
31	92643.0
32	118596.0
33	168276.0
34	273444.0
35	499994.0
36	1139354.0
37	2711954.0
38	4210825.0
39	1823051.0
40	13924.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.558415357141516	30.620326615301146	29.544306151311105	20.276951876246233
2	20.85719312265031	30.29123021385278	29.698145894112937	19.153430769383974
3	20.88516360636497	29.184631573172865	29.80643761385393	20.123767206608232
4	21.089980574402972	28.877681283588387	29.62329296627067	20.409045175737972
5	21.72548359149397	29.295805947387887	29.397214237657565	19.58149622346058
6	21.464332566780183	29.53532832822987	28.89306417609979	20.107274928890156
7	21.26602340960022	29.83974537600384	29.203884210932742	19.690347003463206
8	20.724800664443123	29.861819521021626	29.317495738475802	20.095884076059452
9	20.79316325206053	29.332022569961584	29.725504905666444	20.149309272311438
10-14	20.677867844272967	29.59929856604273	29.494600464752445	20.22823312493185
15-19	20.582015075387332	29.246380356869782	29.653774906527232	20.517829661215657
20-24	20.76624116154509	29.166164821711654	29.432395099030213	20.635198917713048
25-29	20.955680323052974	29.05655436136013	29.214209355139715	20.773555960447183
30-34	21.001053393759705	29.001910504595614	29.160624509893246	20.836411591751435
35-39	21.121818854184156	28.986372524716558	28.97555993832091	20.916248682778367
40-44	21.123171890950175	28.881190535299577	29.03361514106081	20.96202243268944
45-49	21.271559710284034	28.815558789219704	28.983756108034974	20.929125392461284
50-54	21.176242174646912	28.76781412102153	28.918615698923478	21.137328005408083
55-59	21.208608759262145	28.754319340644123	28.99089325310636	21.04617864698737
60-64	21.149544951152983	28.80958250253193	28.918310988102323	21.12256155821276
65-69	21.103408084906327	29.055779746776484	28.747535623790586	21.093276544526603
70-74	21.166009765122006	29.04058754428839	28.71648780764662	21.076914882942983
75-79	21.164188668987364	29.003167888257558	28.75254236849818	21.080101074256895
80-84	21.178150175460765	29.00278071516389	28.75938012884518	21.059688980530172
85-89	21.158203390319127	29.003934216854105	28.72360761614064	21.114254776686128
90-94	21.177194218772083	29.0351339635163	28.6390985597669	21.14857325794471
95-99	21.23529855230821	29.159027354762806	28.47047462986111	21.135199463067877
100-101	21.362404235059795	29.201060512085817	28.31902719418553	21.117508058668857
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.83536723338775
2	40.01062389203428
3	41.008930812973205
4	41.499025750140945
5	41.306979814954545
6	41.57160749567034
7	40.95637041306342
8	40.82068474050257
9	40.94247252437197
10-14	40.90610096920482
15-19	41.09984473660299
20-24	41.40144007925814
25-29	41.72923628350016
30-34	41.83746498551114
35-39	42.03806753696252
40-44	42.08519432363961
45-49	42.200685102745325
50-54	42.313570180054995
55-59	42.25478740624952
60-64	42.27210650936574
65-69	42.19668462943293
70-74	42.24292464806499
75-79	42.24428974324426
80-84	42.23783915599093
85-89	42.272458167005254
90-94	42.32576747671679
95-99	42.370498015376086
100-101	42.47991229372865
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	396.0
1	355.0
2	352.5
3	455.5
4	565.5
5	596.5
6	662.0
7	773.0
8	929.5
9	1232.0
10	1661.5
11	2191.5
12	3008.0
13	4129.0
14	5479.5
15	7252.5
16	9722.0
17	13058.5
18	17472.0
19	23537.5
20	31667.0
21	42331.5
22	56329.5
23	73718.0
24	94440.5
25	118339.0
26	144715.5
27	173076.5
28	202557.0
29	232592.0
30	263077.5
31	291515.5
32	317661.0
33	342663.5
34	364506.5
35	382653.5
36	398372.5
37	409419.0
38	415095.5
39	415515.5
40	410133.5
41	401573.0
42	389246.0
43	375417.0
44	362907.5
45	350710.0
46	340919.5
47	332591.0
48	323962.5
49	314507.0
50	301233.5
51	285323.0
52	267759.5
53	250637.5
54	235424.0
55	220500.0
56	205992.0
57	190156.5
58	172137.0
59	152480.0
60	131181.0
61	109850.5
62	90833.5
63	74742.5
64	60929.0
65	49112.0
66	39596.5
67	31636.5
68	24687.0
69	19225.0
70	14861.0
71	11407.0
72	8940.5
73	7040.0
74	5462.0
75	4398.0
76	3649.0
77	2955.5
78	2416.0
79	1938.0
80	1528.0
81	1190.0
82	904.5
83	642.5
84	426.0
85	253.5
86	156.0
87	102.0
88	53.0
89	40.0
90	35.0
91	25.5
92	23.5
93	16.5
94	11.5
95	12.0
96	7.5
97	3.5
98	2.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12946612715022002
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	2.4063054310433234
15-19	8.560610256205089E-5
20-24	2.026593448407735E-4
25-29	0.0
30-34	1.834416483472519E-4
35-39	1.0657086237316538E-4
40-44	2.09647598111145E-4
45-49	0.0013627093877224426
50-54	2.0790053479355215E-4
55-59	2.4284180114540966E-4
60-64	0.0
65-69	0.0031604375415255114
70-74	0.019490238371066115
75-79	0.016252930043566517
80-84	0.0013225269314178065
85-89	0.00678734098884832
90-94	0.00809414435040779
95-99	0.004586041208681298
100-101	0.004210422595398829
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.1447782E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	4.758489118685764
#Duplication Level	Relative count
1	100.0
2	3.7467835400933036
3	0.27405284408275976
4	0.14957846070165895
5	0.10669232861237213
6	0.07112821907491475
7	0.0596221836363256
8	0.031903098261542646
9	0.02562707893140311
10++	0.13493441559800004
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC	28239	0.246676605127526	TruSeq Adapter, Index 4 (100% over 50bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	8310655	3.6216133	4.5918503	65-69
TTTTT	7863115	3.4696789	4.135772	15-19
CCCAG	2114275	3.451042	3.9443674	25-29
CTGGG	2034810	3.26339	3.503137	80-84
GGAGG	2046050	3.2404935	3.587519	4
CCTCC	1948070	3.2199082	3.7525291	65-69
CCAGG	1891995	3.0573428	3.4027522	25-29
GGGGG	1378510	3.014476	3.5231748	70-74
GGAAG	1829395	2.098434	6.130299	5
GAGCA	1203830	1.3948183	5.2769976	9
AGAGC	1201910	1.3925937	5.357962	8
>>END_MODULE