FastQCFastQC Report
Mon 21 Jul 2014
ZL1_CGATGT_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL1_CGATGT_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12906465
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC 79581 0.6165979607894183 TruSeq Adapter, Index 2 (100% over 50bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCCAG 2550695 3.724268 4.336115 25-29
CTGGG 2445860 3.518418 3.7749634 70-74
AAAAA 9119410 3.4300857 5.62669 65-69
CCAGG 2286485 3.3072739 3.7438 25-29
GGAGG 2320115 3.2934425 3.6874993 70-74
CCTCC 2195030 3.2478662 3.7963243 70-74
CCTGG 2154725 3.1288755 3.402336 70-74
TTTTT 8135325 3.1202292 3.7810225 10-14
GGAAG 2041300 2.0789633 10.501784 5
GAAGA 2137890 1.5621572 7.6352763 6
AAGAG 2132910 1.5585183 7.5604815 7
AGAGC 1418885 1.458706 9.828617 8
GAGCA 1370885 1.409359 9.797208 9
GATCG 433455 0.44736224 8.882546 1
CGGAA 404000 0.41533828 8.882384 4
TCGGA 361105 0.3726909 8.863615 3
ATCGG 352935 0.36425877 8.851104 2
Produced by FastQC (version 0.10.1)