##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL1_CGATGT_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	12906465	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.19279260432659	34.0	33.0	34.0	31.0	34.0
2	33.36286791154666	34.0	34.0	34.0	31.0	34.0
3	33.4047587778683	34.0	34.0	34.0	31.0	34.0
4	36.64885218376992	37.0	37.0	37.0	35.0	37.0
5	36.59125841196641	37.0	37.0	37.0	35.0	37.0
6	36.59428859877588	37.0	37.0	37.0	35.0	37.0
7	36.59065274651115	37.0	37.0	37.0	35.0	37.0
8	36.58475299007125	37.0	37.0	37.0	35.0	37.0
9	38.45895587986331	39.0	39.0	39.0	37.0	39.0
10-14	38.76717813901793	39.4	39.2	39.4	37.2	39.4
15-19	40.05767447554384	41.0	40.0	41.0	38.0	41.0
20-24	39.9961850591932	41.0	40.0	41.0	38.0	41.0
25-29	39.86035609285734	41.0	40.0	41.0	38.0	41.0
30-34	39.692442539455996	41.0	40.0	41.0	38.0	41.0
35-39	39.50335167685343	41.0	40.0	41.0	37.0	41.0
40-44	39.3073128544493	41.0	39.2	41.0	36.4	41.0
45-49	39.160854501987956	40.4	39.0	41.0	36.0	41.0
50-54	39.09591207197324	40.6	39.0	41.0	35.4	41.0
55-59	38.79105539743067	40.0	38.2	41.0	35.0	41.0
60-64	38.51029239997164	40.0	37.6	41.0	35.0	41.0
65-69	37.90094263611299	39.4	36.4	41.0	34.2	41.0
70-74	37.04753448756108	38.0	35.0	40.4	34.0	41.0
75-79	35.83897226699952	36.2	34.8	39.0	32.4	40.2
80-84	35.414300677993545	35.6	35.0	37.6	33.0	39.2
85-89	34.69786837836696	35.0	35.0	36.4	33.0	37.8
90-94	34.2027527134657	35.0	35.0	35.6	32.6	36.6
95-99	33.89591915369545	35.0	34.8	35.0	32.0	36.0
100-101	33.53159966729852	35.0	34.0	35.0	31.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	8.0
11	12.0
12	37.0
13	80.0
14	413.0
15	1317.0
16	3148.0
17	5781.0
18	8362.0
19	10249.0
20	11797.0
21	13329.0
22	15553.0
23	18260.0
24	22080.0
25	26749.0
26	33265.0
27	42531.0
28	56490.0
29	83586.0
30	100525.0
31	95346.0
32	118808.0
33	164950.0
34	256691.0
35	446290.0
36	960194.0
37	2551171.0
38	5202968.0
39	2637823.0
40	18648.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.581426707196535	30.846960958193097	29.418415021498518	20.153197313111846
2	20.763160168179283	30.713181339739425	29.619496895548085	18.90416159653321
3	20.746697415221558	29.228493199423607	30.129766567083045	19.895042818271794
4	20.84639204540612	28.966507764255	29.618019015912505	20.569081174426376
5	21.85176963637991	29.33084310847316	29.464086409407997	19.35330084573894
6	21.586285777332932	29.579621810096768	28.899325033901718	19.93476737866858
7	21.07346202077796	30.183105908550484	29.26744852289144	19.475983547780125
8	20.511821013732266	30.19249655114704	29.346439943082785	19.949242492037904
9	20.965097724280042	29.29687563558263	29.799910355004254	19.93811628513307
10-14	20.460885300506373	29.665830264135067	29.628853446702873	20.244430988655683
15-19	20.471242007967025	29.31668181026118	29.751143750096364	20.460932431675424
20-24	20.65341441242309	29.30762675188467	29.499458069129382	20.53950076656286
25-29	20.84830819283204	29.13642891372657	29.273364937649465	20.741897955791924
30-34	20.846823927953604	29.06897095958373	29.16720547948814	20.916999632974523
35-39	21.168250967613137	28.98996063162034	29.235685842459507	20.60610255830701
40-44	20.974968828767775	28.932322498307744	29.18758243357919	20.90512623934529
45-49	21.254906469861375	28.87793586265697	29.118636078084503	20.74852158939716
50-54	21.074936443841867	28.77303348513897	28.98384884753602	21.168181223483142
55-59	21.116670549290646	28.745762450264102	29.19317152205415	20.944395478391108
60-64	21.059794685812168	28.889998399527716	29.044108941705986	21.006097972954127
65-69	20.93720950082351	29.4014222337598	28.73182172880068	20.929546536616016
70-74	21.021289167744893	29.35396108110323	28.70210399127704	20.922645759874836
75-79	21.02881505570821	29.249168045379196	28.76832713313078	20.953689765781814
80-84	21.04646342731699	29.223297105690133	28.76669500161237	20.963544465380508
85-89	21.01287724023906	29.187219992152542	28.76130021257943	21.03860255502897
90-94	21.027393230882225	29.18998315528278	28.700013768121647	21.08260984571335
95-99	21.079447220993544	29.290702846554566	28.5778220959419	21.052027836509986
100-101	21.16685664825066	29.30991299407787	28.43985764688886	21.08337271078261
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.73462402030838
2	39.66732176471249
3	40.64174023349335
4	41.41547321983249
5	41.20507048211884
6	41.521053156001514
7	40.549445568558085
8	40.46106350577017
9	40.90321400941311
10-14	40.705316289162056
15-19	40.93217443964245
20-24	41.19291517898595
25-29	41.59020614862396
30-34	41.763823560928124
35-39	41.77435352592015
40-44	41.880095068113064
45-49	42.00342805925853
50-54	42.24311766732501
55-59	42.061066027681754
60-64	42.06589265876629
65-69	41.86675603743952
70-74	41.94393492761973
75-79	41.982504821490025
80-84	42.0100078926975
85-89	42.05147979526803
90-94	42.110003076595575
95-99	42.13147505750353
100-101	42.25022935903327
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	423.0
1	354.5
2	323.0
3	418.0
4	507.0
5	566.5
6	622.0
7	702.0
8	844.0
9	1027.5
10	1250.0
11	1582.5
12	2060.5
13	2717.0
14	3653.5
15	5080.5
16	7197.0
17	10449.5
18	15274.0
19	22124.5
20	31427.5
21	44244.5
22	60442.0
23	80470.0
24	104900.0
25	133316.5
26	165203.0
27	198335.5
28	232848.5
29	269119.0
30	303722.0
31	336648.0
32	369067.0
33	398429.0
34	423799.0
35	444407.5
36	462328.0
37	476137.0
38	481892.0
39	481459.5
40	475072.0
41	462626.0
42	446756.0
43	428595.5
44	410499.5
45	394961.5
46	381756.5
47	371704.5
48	361779.0
49	349412.5
50	334506.5
51	315898.0
52	295461.5
53	275704.5
54	257032.5
55	241091.5
56	226083.0
57	208653.0
58	188505.0
59	165443.5
60	141035.5
61	117557.0
62	96633.0
63	78512.5
64	63589.5
65	51335.0
66	40879.5
67	32377.0
68	25511.5
69	19899.5
70	15577.5
71	12163.5
72	9473.0
73	7498.5
74	5947.5
75	4763.5
76	3842.5
77	3186.0
78	2639.0
79	2061.5
80	1579.5
81	1172.0
82	829.0
83	584.0
84	380.5
85	248.0
86	159.0
87	98.5
88	60.0
89	41.0
90	34.5
91	25.0
92	15.5
93	14.5
94	11.5
95	6.5
96	7.0
97	7.5
98	4.0
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13330528537442282
2	0.0
3	4.5713524191170855E-4
4	8.522860442421686E-5
5	0.0
6	3.02174142958587E-4
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	1.394649890578094E-4
20-24	1.689075978589025E-4
25-29	0.0
30-34	3.099221979062431E-5
35-39	1.2086965718343481E-4
40-44	1.0382393629859145E-4
45-49	2.866780330632749E-4
50-54	4.0289885727811604E-5
55-59	0.0018967238511862078
60-64	0.002127615888626359
65-69	0.0017990983588457412
70-74	0.018669713201872086
75-79	0.016700157634177912
80-84	0.004735611184007395
85-89	0.0068895704594557836
90-94	0.01000583815940306
95-99	0.007721711560834048
100-101	0.0030178674021120425
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.2906465E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.766755938923872
#Duplication Level	Relative count
1	100.0
2	4.304221965610516
3	0.3132068905515921
4	0.16609456317129886
5	0.11652983638367317
6	0.09280204164491619
7	0.07487437450896647
8	0.05800127602807262
9	0.05114657977020949
10++	0.2799879779173324
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	79581	0.6165979607894183	TruSeq Adapter, Index 2 (100% over 50bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	2550695	3.724268	4.336115	25-29
CTGGG	2445860	3.518418	3.7749634	70-74
AAAAA	9119410	3.4300857	5.62669	65-69
CCAGG	2286485	3.3072739	3.7438	25-29
GGAGG	2320115	3.2934425	3.6874993	70-74
CCTCC	2195030	3.2478662	3.7963243	70-74
CCTGG	2154725	3.1288755	3.402336	70-74
TTTTT	8135325	3.1202292	3.7810225	10-14
GGAAG	2041300	2.0789633	10.501784	5
GAAGA	2137890	1.5621572	7.6352763	6
AAGAG	2132910	1.5585183	7.5604815	7
AGAGC	1418885	1.458706	9.828617	8
GAGCA	1370885	1.409359	9.797208	9
GATCG	433455	0.44736224	8.882546	1
CGGAA	404000	0.41533828	8.882384	4
TCGGA	361105	0.3726909	8.863615	3
ATCGG	352935	0.36425877	8.851104	2
>>END_MODULE