FastQCFastQC Report
Mon 21 Jul 2014
ZL1_CGATGT_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename ZL1_CGATGT_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13280025
Filtered Sequences 0
Sequence length 101
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC 68529 0.5160306550627728 TruSeq Adapter, Index 2 (100% over 50bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCCAG 2599050 3.7192237 4.312791 25-29
CTGGG 2497610 3.518772 3.7935221 1
AAAAA 9331440 3.4216545 5.6120477 65-69
CCAGG 2332880 3.3052142 3.720733 25-29
GGAGG 2373775 3.2967484 3.6454194 70-74
CCTCC 2237410 3.2479064 3.7974238 70-74
CCTGG 2198225 3.1280174 3.3967369 70-74
TTTTT 8270265 3.099245 3.7262764 15-19
GGAAG 2085585 2.076273 10.300786 5
GAAGA 2186905 1.5606188 7.436434 6
AAGAG 2180050 1.5557269 7.4123645 7
AGAGC 1443300 1.4512547 9.631072 8
GAGCA 1387355 1.3950014 9.399167 9
GATCG 441820 0.4461924 8.714833 1
CGGAA 411410 0.41367742 8.700136 4
TCGGA 367540 0.37117732 8.68259 3
ATCGG 358260 0.3618055 8.67091 2