##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ZL1_CGATGT_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	13280025	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.23049406910002	34.0	33.0	34.0	31.0	34.0
2	33.374980845292086	34.0	34.0	34.0	31.0	34.0
3	33.400094051027764	34.0	34.0	34.0	31.0	34.0
4	36.64688793884048	37.0	37.0	37.0	35.0	37.0
5	36.59081921909033	37.0	37.0	37.0	35.0	37.0
6	36.58360530194785	37.0	37.0	37.0	35.0	37.0
7	36.58558135244474	37.0	37.0	37.0	35.0	37.0
8	36.58189047083872	37.0	37.0	37.0	35.0	37.0
9	38.450854572939434	39.0	39.0	39.0	37.0	39.0
10-14	36.885784627664485	39.4	38.4	39.4	26.2	39.4
15-19	39.5510537066007	41.0	39.0	41.0	36.6	41.0
20-24	39.990435018006366	41.0	40.0	41.0	38.0	41.0
25-29	39.85402504889863	41.0	40.0	41.0	38.0	41.0
30-34	39.691649887707285	41.0	40.0	41.0	38.0	41.0
35-39	39.510349641661065	41.0	40.0	41.0	37.0	41.0
40-44	39.31794167556161	41.0	39.2	41.0	36.4	41.0
45-49	39.168843688170774	40.4	39.0	41.0	36.0	41.0
50-54	39.095890587555374	40.4	39.0	41.0	35.4	41.0
55-59	38.78610543278345	40.0	38.2	41.0	35.0	41.0
60-64	38.50074699407569	40.0	37.4	41.0	35.0	41.0
65-69	37.88921163928532	39.2	36.4	41.0	34.2	41.0
70-74	37.01876296166611	38.0	35.0	40.2	34.0	41.0
75-79	35.80677626736396	36.2	34.8	39.0	32.4	40.0
80-84	35.37168175511718	35.6	35.0	37.4	33.0	39.2
85-89	34.67650088008118	35.0	35.0	36.4	33.0	37.6
90-94	34.18758154446245	35.0	35.0	35.6	32.6	36.6
95-99	33.90763419496575	35.0	34.8	35.0	32.0	36.0
100-101	33.56122891334918	35.0	34.0	35.0	31.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	10.0
12	26.0
13	107.0
14	448.0
15	1355.0
16	3288.0
17	6087.0
18	8668.0
19	10270.0
20	11744.0
21	13164.0
22	15297.0
23	18200.0
24	22037.0
25	27088.0
26	34187.0
27	43755.0
28	59285.0
29	86419.0
30	102708.0
31	101179.0
32	129641.0
33	180722.0
34	286889.0
35	519645.0
36	1185357.0
37	3056928.0
38	5092049.0
39	2248156.0
40	15309.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.520972485793536	30.80626056955531	29.48817529232855	20.184591652322606
2	20.736256144096114	30.728097273913264	29.64712792332846	18.88851865866216
3	20.76153471096628	29.242482600748115	30.116660171949977	19.879322516335623
4	20.841564680789382	28.96403432975465	29.641510464023973	20.552890525431994
5	21.80936406369717	29.35081824017651	29.500245669718243	19.339572026408085
6	21.5708705367648	29.58159340814494	28.933492218576397	19.914043836513862
7	21.035502568707514	30.183489865418174	29.30501260351543	19.475994962358882
8	20.5025065841367	30.175342290394784	29.40034374935288	19.921807376115634
9	20.95410211953667	29.321834861003648	29.79039572591166	19.933667293548016
10-14	20.57880966333037	29.723437729281155	29.517629910948195	20.180122696440282
15-19	20.472729342276118	29.32517673513793	29.76486454448909	20.437229378096863
20-24	20.64931331556183	29.32149237743195	29.51076666765261	20.518427639353607
25-29	20.863829699115776	29.13702195590746	29.281318371012105	20.71782997396466
30-34	20.841234061482954	29.09717406919199	29.164869854363552	20.896722014961504
35-39	21.167077148396555	28.997910723898446	29.245154580648396	20.589857547056603
40-44	20.963580450710253	28.954684607068813	29.18196624042067	20.899768701800262
45-49	21.243608622017206	28.884237763161607	29.131127719004162	20.741025895817025
50-54	21.07135320030915	28.785852387033632	28.987883992241148	21.15491042041607
55-59	21.104610037810858	28.76951642271678	29.20193977178546	20.923933767686904
60-64	21.046988992867107	28.90992750390154	29.04853868874494	20.994544814486417
65-69	20.93407589809832	29.40438604630589	28.75458188532004	20.906956170275745
70-74	21.005508964450943	29.363669092907436	28.721644201908568	20.90917774073305
75-79	21.01833676890808	29.266130687684715	28.772020670445286	20.94351187296192
80-84	21.038502385168613	29.22693042692068	28.791047747514288	20.943519440396418
85-89	20.998091543871922	29.201472273301192	28.775781112930858	21.024655069896024
90-94	21.019498610682554	29.19759283075537	28.70993741335263	21.072971145209447
95-99	21.074322902800454	29.28713763637238	28.59354763580142	21.044991825025747
100-101	21.162259898092834	29.305800143146104	28.46365240038934	21.068287558371722
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.705564138116145
2	39.62477480275828
3	40.64085722730191
4	41.39445520622137
5	41.14893609010525
6	41.48491437327866
7	40.51149753106639
8	40.424313960252334
9	40.88776941308469
10-14	40.75893235977065
15-19	40.90995872037298
20-24	41.16774095491544
25-29	41.58165967308044
30-34	41.73795607644446
35-39	41.756934695453154
40-44	41.863349152510516
45-49	41.98463451783423
50-54	42.22626362072522
55-59	42.02854380549776
60-64	42.041533807353524
65-69	41.84103206837406
70-74	41.91468670518399
75-79	41.96184864187
80-84	41.98202182556503
85-89	42.02274661376794
90-94	42.092469755892
95-99	42.11931472782619
100-101	42.230547456464556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	416.0
1	369.5
2	330.0
3	383.5
4	479.0
5	578.5
6	669.5
7	755.0
8	883.5
9	1086.5
10	1327.5
11	1641.5
12	2149.5
13	2807.5
14	3786.5
15	5296.5
16	7514.0
17	10861.0
18	15828.0
19	23007.5
20	32801.5
21	45871.0
22	62891.5
23	83948.0
24	109376.5
25	138681.5
26	171000.5
27	206279.5
28	242504.0
29	278796.0
30	314932.0
31	349653.0
32	382195.0
33	411343.0
34	437538.0
35	460090.5
36	477749.0
37	490578.0
38	497089.0
39	495697.5
40	488177.5
41	475799.0
42	458847.0
43	440257.0
44	422451.5
45	406267.5
46	393408.5
47	382728.0
48	371546.5
49	358151.0
50	342492.0
51	323398.5
52	302325.5
53	282247.0
54	263670.0
55	247285.5
56	231043.0
57	213097.0
58	192247.0
59	168380.0
60	143164.5
61	119046.0
62	97404.0
63	79020.5
64	63938.5
65	51717.5
66	41311.0
67	32667.0
68	25680.0
69	19969.5
70	15684.5
71	12211.5
72	9560.5
73	7632.0
74	6154.0
75	4983.0
76	4014.5
77	3253.5
78	2626.0
79	2063.0
80	1580.0
81	1150.5
82	805.5
83	573.0
84	368.0
85	211.5
86	140.0
87	94.0
88	55.5
89	34.0
90	32.0
91	28.0
92	17.0
93	11.5
94	9.0
95	7.0
96	6.5
97	4.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12571512478327412
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	2.407236432160331
15-19	8.734923315279904E-5
20-24	2.9066210342224507E-4
25-29	0.0
30-34	1.912647001794048E-4
35-39	1.400599772967295E-4
40-44	1.912647001794048E-4
45-49	0.0013659612839584264
50-54	1.7771050882810837E-4
55-59	2.2439716792701822E-4
60-64	0.0
65-69	0.0029608377996276363
70-74	0.01884333802082451
75-79	0.015748464328945163
80-84	0.0012590337744093102
85-89	0.006885529206458572
90-94	0.007969864514562285
95-99	0.004478907230972834
100-101	0.004408877242324468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.3280025E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.373242513305263
#Duplication Level	Relative count
1	100.0
2	3.822645211725017
3	0.3100718254363828
4	0.16421739655091003
5	0.12067093037287317
6	0.07922308907088631
7	0.09181483832718611
8	0.059286152748411605
9	0.04564509105408681
10++	0.2376692672126589
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	68529	0.5160306550627728	TruSeq Adapter, Index 2 (100% over 50bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCCAG	2599050	3.7192237	4.312791	25-29
CTGGG	2497610	3.518772	3.7935221	1
AAAAA	9331440	3.4216545	5.6120477	65-69
CCAGG	2332880	3.3052142	3.720733	25-29
GGAGG	2373775	3.2967484	3.6454194	70-74
CCTCC	2237410	3.2479064	3.7974238	70-74
CCTGG	2198225	3.1280174	3.3967369	70-74
TTTTT	8270265	3.099245	3.7262764	15-19
GGAAG	2085585	2.076273	10.300786	5
GAAGA	2186905	1.5606188	7.436434	6
AAGAG	2180050	1.5557269	7.4123645	7
AGAGC	1443300	1.4512547	9.631072	8
GAGCA	1387355	1.3950014	9.399167	9
GATCG	441820	0.4461924	8.714833	1
CGGAA	411410	0.41367742	8.700136	4
TCGGA	367540	0.37117732	8.68259	3
ATCGG	358260	0.3618055	8.67091	2
>>END_MODULE