##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SDW_HiSeq_6_GTGAAA_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	22169699	
Filtered Sequences	0	
Sequence length	101	
%GC	46	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01094985547616	34.0	33.0	34.0	31.0	34.0
2	33.17758459417965	34.0	33.0	34.0	31.0	34.0
3	33.22874951978374	34.0	34.0	34.0	31.0	34.0
4	36.54492846294395	37.0	37.0	37.0	35.0	37.0
5	36.414924713231336	37.0	37.0	37.0	35.0	37.0
6	36.40035563856776	37.0	37.0	37.0	35.0	37.0
7	36.388152901850404	37.0	37.0	37.0	35.0	37.0
8	36.41187983652823	37.0	37.0	37.0	35.0	37.0
9	38.26047493924027	39.0	39.0	39.0	37.0	39.0
10-14	38.50730067196672	39.4	39.2	39.4	37.2	39.4
15-19	39.74813224121807	41.0	40.0	41.0	38.0	41.0
20-24	39.679778214399754	41.0	40.0	41.0	37.4	41.0
25-29	39.55499960554268	41.0	39.8	41.0	37.0	41.0
30-34	39.40072256280972	41.0	39.0	41.0	36.8	41.0
35-39	39.22862819202011	40.0	39.0	41.0	36.0	41.0
40-44	39.003249895273726	40.0	38.4	41.0	35.4	41.0
45-49	38.78924500508555	40.0	38.0	41.0	35.0	41.0
50-54	38.615313423966654	40.0	37.8	41.0	35.0	41.0
55-59	38.0486920097562	39.4	36.6	41.0	34.0	41.0
60-64	37.429590667875104	38.6	35.4	40.6	34.0	41.0
65-69	36.533232453900254	36.8	35.0	39.4	33.0	41.0
70-74	35.64667100802767	35.4	35.0	38.0	32.8	40.0
75-79	34.60532365369507	35.0	34.2	36.2	31.4	39.0
80-84	34.53420396912019	35.0	35.0	35.6	32.0	37.0
85-89	34.169108520598314	35.0	34.2	35.0	32.0	36.0
90-94	33.940460896649974	35.0	34.0	35.0	32.0	36.0
95-99	33.7758033070273	35.0	34.0	35.0	31.0	35.0
100-101	33.29006686107917	35.0	33.5	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	4.0
13	26.0
14	136.0
15	430.0
16	1053.0
17	2060.0
18	3569.0
19	5846.0
20	8862.0
21	12981.0
22	18725.0
23	26574.0
24	36797.0
25	49866.0
26	65751.0
27	86735.0
28	112010.0
29	144844.0
30	185012.0
31	241592.0
32	321642.0
33	446716.0
34	661564.0
35	1164895.0
36	2794329.0
37	7469130.0
38	7039207.0
39	1265320.0
40	4018.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.71571264764266	12.479499829486093	14.005191697962175	42.79959582490906
2	29.03953900636441	10.460265163087401	45.497027313478675	15.00316851706951
3	30.53838484681276	18.98816488216642	25.98892749964715	24.484522771373666
4	32.98708746564398	25.454089385697117	23.15270947070594	18.406113677952955
5	28.56801528969789	31.507829673285144	26.50262414478428	13.421530892232683
6	24.813034222972536	29.124671471633423	27.858023692608548	18.204270612785496
7	17.02607248553239	27.377758456104917	41.772418770074985	13.823750288287709
8	22.529430823575908	19.333068076386603	37.447563000291524	20.689938099745962
9	23.602034470562728	15.544153305825217	37.48662983651695	23.367182387095106
10-14	22.855872417289845	26.219079752798063	30.208693530035607	20.716354299876485
15-19	23.19112490726514	24.931624561729794	29.805073031171148	22.072177499833916
20-24	22.80129398961433	25.16599819569944	29.68987382002682	22.342833994659415
25-29	23.50104945927008	25.301831912458677	29.056652577438065	22.140466050833176
30-34	23.547408454953153	25.312982191134253	28.874578522548322	22.265030831364275
35-39	23.844088006235083	25.160749573950703	28.71053608559771	22.2846263342165
40-44	24.110059620733995	25.516574985890344	28.485207295005015	21.888158098370646
45-49	24.033288124567438	25.499494133765904	27.715457710855762	22.751760030810896
50-54	23.889759251672825	25.38654477624321	28.448833994583218	22.274861977500752
55-59	23.943408263844933	25.131357376487422	28.596095208709144	22.3291391509585
60-64	24.452513192744483	24.81305106294713	28.633597188223032	22.100838556085346
65-69	24.334840590634215	25.15408652438286	28.908197197407898	21.60287568757503
70-74	24.0405111032873	25.162345019576232	28.667866908902194	22.129276968234272
75-79	23.71556453867416	25.220454846167577	28.839626596477082	22.224354018681183
80-84	24.072921757253514	25.39448493959138	28.65500880366108	21.877584499494027
85-89	23.83673963767184	25.421304195307442	28.831963824128092	21.90999234289262
90-94	24.104481430713562	24.886800963266758	28.504071026127665	22.504646579892015
95-99	23.898951161498722	25.310077126689762	28.80392477063444	21.987046941177077
100-101	25.22617720507086	25.517332901680817	27.644089118502123	21.6124007747462
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	73.51530847255174
2	44.04270752343392
3	55.02290761818642
4	51.39320114359695
5	41.98954618193057
6	43.01730483575803
7	30.849822773820097
8	43.21936892332187
9	46.969216857657834
10-14	43.57222671716633
15-19	45.26330240709906
20-24	45.14412798427374
25-29	45.64151551010326
30-34	45.81243928631743
35-39	46.12871434045158
40-44	45.99821771910464
45-49	46.785048155378334
50-54	46.16462122917358
55-59	46.272547414803434
60-64	46.55335174882983
65-69	45.937716278209244
70-74	46.16978807152157
75-79	45.93991855735534
80-84	45.950506256747545
85-89	45.74673198056446
90-94	46.60912801060558
95-99	45.8859981026758
100-101	46.83857797981706
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	79.0
1	164.0
2	230.5
3	244.0
4	264.0
5	265.0
6	273.5
7	254.0
8	237.5
9	251.0
10	267.5
11	317.5
12	418.0
13	530.0
14	603.5
15	760.5
16	1208.5
17	1985.0
18	3410.0
19	5170.5
20	7869.5
21	13400.0
22	19874.0
23	29275.5
24	39564.0
25	47670.5
26	59853.0
27	78537.0
28	102183.5
29	126703.0
30	156604.0
31	195613.5
32	236184.0
33	279080.5
34	322008.5
35	362413.0
36	404401.0
37	452614.5
38	494895.5
39	519678.0
40	530578.0
41	597141.0
42	701184.0
43	852477.5
44	1003695.0
45	1143505.5
46	1202015.5
47	1119607.5
48	1065800.0
49	1109721.5
50	1202828.0
51	1253002.0
52	1259828.5
53	1156972.0
54	979025.0
55	842251.5
56	749863.0
57	609228.5
58	394002.5
59	220633.0
60	118601.5
61	55850.5
62	22878.0
63	7759.5
64	3384.0
65	1472.0
66	636.5
67	213.5
68	79.5
69	39.5
70	23.5
71	20.0
72	17.0
73	12.5
74	9.0
75	5.5
76	3.0
77	2.0
78	3.0
79	2.5
80	1.0
81	1.0
82	1.0
83	0.5
84	1.0
85	1.0
86	0.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12544148659844231
2	9.923454531340277E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	2.7063966903655298E-5
8	0.0
9	0.0
10-14	4.483597183705562E-4
15-19	1.9034990055570895E-4
20-24	9.021322301218434E-7
25-29	4.131765613958042E-4
30-34	3.022142970908175E-4
35-39	0.0019549205426740344
40-44	5.845816851189545E-4
45-49	0.0018024601957834431
50-54	7.397484286999115E-5
55-59	0.002180453600204495
60-64	0.002452897533701292
65-69	0.0015182885432950624
70-74	0.01431503422757341
75-79	0.012124657172837575
80-84	0.002313969170262528
85-89	0.002218343153869613
90-94	0.005123208934861949
95-99	0.004532312324132141
100-101	0.0028529931777603293
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.2169699E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.39871611014853
#Duplication Level	Relative count
1	100.0
2	90.15829318651066
3	76.668960770819
4	57.378822141382365
5	44.69570347065185
6	34.60328384622947
7	28.635335758529152
8	24.14708484908072
9	21.325336741716644
10++	505.5746730901583
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGATAATCTCGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGC	66060	0.2979742756092449	No Hit
CCCGTGATAGCCTACCTAGGTAAAGACCCTAAGTACAAGCTGATAGGTCA	29712	0.13402076410690106	No Hit
AGCAAACTCGCAGAGCTGGAATTACCGCAGCTGCTGGCACCAGACTGGCC	26866	0.12118342247226721	No Hit
GCCAGATCTTTTCCATATCATCCCAGTTGTTGACGATACCGTGTTCAATT	26482	0.11945132859043327	No Hit
GTTATTGCTACCCTCTTCCACCTGCTAAAATCGCAGGGAGTCCCAATTAG	25540	0.1152022857865594	No Hit
CCCTGGTGTGCCCTTCCGTCAATTCCTTCAAGTTTCAGCCTTGCGGTCGT	24740	0.11159375686607204	No Hit
GTAATCTTGAAGTGTGTTGCCGTCTTCAAGTTGCTTGCCGGCAAAGATCA	23799	0.10734922472334875	No Hit
CCTGGTTGCAGATGTACTTCTCGCCACCATAGTATAAGCCAGAGAGAGCT	23432	0.10569381208107517	No Hit
CCCGTACGGTCCTCCATCAGAGTTTCCCCTGACTTCAACCTGTGCAGGCA	23288	0.10504427687538744	No Hit
GCGATAATCTCGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACG	23124	0.10430452844668753	No Hit
CTTCCGTCAATTCCTTCAAGTTTCAGCCTTGCGGTCGTAGTTCCCCCAGA	22663	0.1022251136562567	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACCA	6048065	4.263828	7.083074	50-54
CTTCT	10269965	4.040078	6.5173516	6
CTTGA	8162080	3.4163375	12.247324	1
CCTTG	7132915	3.3843212	4.1528144	30-34
TCTTC	7935355	3.121671	5.540113	7
CCTTC	5894805	3.062251	4.138061	10-14
TTCTT	10095275	3.0073812	5.66741	6
CCACC	3760725	3.00538	5.0897045	20-24
GAAGA	5108120	2.6501231	5.0486217	3
TCTTG	6821535	2.4509592	5.0871983	7
CTGGA	4854525	2.4507012	17.807636	1
TGGAA	5401615	2.405596	9.764945	2
CAGCC	3177715	2.3194015	5.5617166	9
CAACA	3643780	2.2661607	5.8012896	9
CTTTG	6111075	2.1956928	5.498669	1
TGGAG	4677835	2.1568592	6.874332	2
CTGTG	4951560	2.1457534	6.048252	1
GGATG	4393365	2.0256956	5.4841666	4
TCAAC	3792310	2.024587	5.721136	8
TGAAG	4507305	2.0073173	5.1839747	8
CCTGG	3496425	2.0008407	9.306797	1
CTGGG	3634330	1.8995285	19.08719	1
TGGGA	4098460	1.8897204	10.813383	2
CTTGT	5119260	1.8393364	5.8467193	1
TTGAA	4912405	1.8138839	6.5173826	7
CTGCT	3735415	1.7723252	5.8588576	1
GTTGA	4588900	1.7542912	5.7410307	1
TGGAT	4580545	1.7510973	5.5374093	2
CTGGC	2914115	1.6676118	7.725192	1
GATGT	4309260	1.6473877	5.311964	5
GCAGA	2708150	1.5926572	5.132653	8
GTGGA	3391295	1.5636604	6.298959	1
CTGGT	3585705	1.5538615	11.53027	1
CTCTG	3125450	1.4829178	5.8908806	1
GGAAT	3140320	1.3985337	6.5285316	3
CTGAA	2840230	1.3849028	6.2670727	1
GGGAA	2540295	1.3644792	6.067837	3
GTTGG	3335350	1.3201168	5.3113723	1
CTGAG	2538015	1.2812617	6.2717824	1
GAATT	3411255	1.2595909	5.368513	4
GTGAA	2818525	1.2552232	5.6309934	1
AATCT	3032885	1.2261343	5.341657	5
TGGTT	3513605	1.1530288	5.458057	2
TGGGT	2714175	1.0742584	5.844362	2
GGGAT	2206280	1.017273	5.393606	3
CTCGC	1586875	0.99425495	5.897894	8
CGGGC	522385	0.36054766	5.0403976	1
>>END_MODULE