FastQCFastQC Report
Mon 21 Jul 2014
SDW_HiSeq_5_CTTGTA_L006_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SDW_HiSeq_5_CTTGTA_L006_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 25658979
Filtered Sequences 0
Sequence length 101
%GC 46

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CGATAATCTCGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGC 90950 0.3544568160720658 No Hit
GTAATCTTGAAGTGTGTTGCCGTCTTCAAGTTGCTTGCCGGCAAAGATCA 48999 0.190962391761574 No Hit
GTAAACGTTTGTCTTGATGTCACCAAGATCGTGGATAGCGACAACTTGGA 43179 0.1682802733499256 No Hit
GCGATAATCTCGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACG 39007 0.15202085788370612 No Hit
CTGAAGAATAACTGGAGACTCTGTCTTGACGGAAGCTGTGATGATAGCCT 36846 0.14359885481024012 No Hit
GGCGATAATCTCGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCAC 31973 0.1246074522294905 No Hit
GTTATTGCTACCCTCTTCCACCTGCTAAAATCGCAGGGAGTCCCAATTAG 28718 0.11192183445802734 No Hit
CTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAACGG 28076 0.10941978634457745 No Hit
CCTGGTTGCAGATGTACTTCTCGCCACCATAGTATAAGCCAGAGAGAGCT 27471 0.10706193726570336 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CACCA 7213270 4.4866686 8.231708 50-54
CTTCT 12080380 4.183248 6.245006 6
CTTGA 9892165 3.583408 11.748986 1
CCTTG 8496315 3.5183504 4.554038 95-97
TCTTC 9474030 3.2807095 5.50335 7
CCTTC 7003615 3.223126 4.3112516 5
TTCTT 12099675 3.1527154 5.914797 6
CCACC 4333925 3.0816033 5.2433457 20-24
GCAGC 5265715 3.0315154 4.2084274 8
AGCCT 6280460 3.0235586 4.036589 9
GAAGA 6094240 2.6848178 5.7956977 3
TCTTG 8402735 2.6182265 5.3192067 7
CTGGA 5598950 2.4254203 14.04073 1
TGGAA 6353410 2.4075968 8.481573 2
CAGCC 3746220 2.3968573 5.7927885 9
CTTTG 7484740 2.3321865 5.2091665 1
CAACA 4171610 2.2698228 5.2461925 9
TGGAG 5730200 2.2335913 7.2791834 2
AAGAA 5133770 2.1987436 5.281314 4
CTGTG 5853305 2.181038 6.5193834 1
CCTGG 4259105 2.1091251 9.361251 1
TGAAG 5522535 2.0927403 5.82835 8
GGATG 5278875 2.0576682 5.413212 4
TCAAC 4310230 2.017299 5.1998844 8
CTGGG 4487340 1.9995234 17.281458 1
GCCTG 3976330 1.9690939 5.3164825 1
TGGGA 5036120 1.963044 11.035883 2
CTTGT 6042680 1.8828518 6.2139025 1
TTGAA 5858270 1.8563966 7.086212 7
TGGAT 5551610 1.8095785 5.103028 2
GTTGA 5241595 1.7085274 5.779912 1
CTGCT 4092940 1.6948992 5.03572 1
GATGT 5084855 1.6574371 5.533782 5
CTGGC 3326440 1.6472657 6.9543943 1
GTGGA 4180990 1.6297204 7.919337 1
CTGGT 4289120 1.5981967 10.781751 1
ATTTC 5157555 1.5623323 5.1350837 5
CTGAA 3615255 1.5225173 5.695645 1
GGCGA 2896810 1.500638 5.1070433 1
GATAA 3864955 1.42385 5.253694 2
GGAAT 3675435 1.3927901 5.77567 3
GGGAA 2994625 1.3570467 6.526839 3
GTTGG 3886805 1.3031917 5.9386873 1
AATCT 3691330 1.2999623 6.087386 5
CTGAG 2967690 1.2855796 5.2979283 1
GAATT 4029540 1.2768999 5.091948 4
GTGAA 3306585 1.2530158 6.1844807 1
TCTCG 3015115 1.2485685 5.14383 7
TGGTT 4063680 1.1393564 5.1582294 2
GTGGG 2737530 1.0976149 6.2295904 1
GGGAT 2691400 1.0490887 5.3290915 3
TGGGT 2974090 0.9971711 5.136582 2
ATCTC 2470110 0.99441516 5.1458383 6
CTCGC 1774055 0.9763321 6.8884172 8
Produced by FastQC (version 0.10.1)