##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SDW_HiSeq_5_CTTGTA_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	25658979	
Filtered Sequences	0	
Sequence length	101	
%GC	46	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00679270987361	34.0	33.0	34.0	31.0	34.0
2	33.16466695732515	34.0	33.0	34.0	31.0	34.0
3	33.22438710441285	34.0	34.0	34.0	31.0	34.0
4	36.544724480268684	37.0	37.0	37.0	35.0	37.0
5	36.41976763767568	37.0	37.0	37.0	35.0	37.0
6	36.410609556989776	37.0	37.0	37.0	35.0	37.0
7	36.39959637521041	37.0	37.0	37.0	35.0	37.0
8	36.42296051608289	37.0	37.0	37.0	35.0	37.0
9	38.27886047219572	39.0	39.0	39.0	37.0	39.0
10-14	38.52470214812522	39.4	39.2	39.4	37.2	39.4
15-19	39.7682601244578	41.0	40.0	41.0	38.0	41.0
20-24	39.70689210977569	41.0	40.0	41.0	37.6	41.0
25-29	39.58533198846298	41.0	40.0	41.0	37.0	41.0
30-34	39.433151272309004	41.0	39.2	41.0	37.0	41.0
35-39	39.26539337360228	40.0	39.0	41.0	36.0	41.0
40-44	39.03730568546784	40.0	38.6	41.0	35.6	41.0
45-49	38.82095805137064	40.0	38.0	41.0	35.0	41.0
50-54	38.64540647544861	40.0	37.8	41.0	35.0	41.0
55-59	38.07116013462578	39.4	36.6	41.0	34.0	41.0
60-64	37.44947075251903	38.6	35.4	40.6	34.0	41.0
65-69	36.561181386055935	36.8	35.0	39.4	33.0	41.0
70-74	35.67665840484143	35.4	35.0	38.0	32.8	40.2
75-79	34.62545851882883	35.0	34.2	36.2	31.4	39.0
80-84	34.54749552583523	35.0	35.0	35.6	32.0	37.0
85-89	34.178467732484606	35.0	34.4	35.0	32.0	36.0
90-94	33.948471519463034	35.0	34.0	35.0	32.0	36.0
95-99	33.7862089758131	35.0	34.0	35.0	31.0	35.0
100-101	33.31441958388134	35.0	33.5	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	0.0
12	5.0
13	38.0
14	153.0
15	486.0
16	1245.0
17	2404.0
18	4352.0
19	6612.0
20	10536.0
21	15308.0
22	21779.0
23	30844.0
24	42349.0
25	57514.0
26	76037.0
27	99803.0
28	127852.0
29	165194.0
30	212489.0
31	274202.0
32	364431.0
33	506052.0
34	750539.0
35	1312571.0
36	3188645.0
37	8603438.0
38	8251093.0
39	1529727.0
40	3277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.61194289188859	13.657427576828345	13.813422159744961	37.917207371538105
2	27.989725065891243	10.487495434341268	46.332776386623486	15.190003113143998
3	30.57668428661951	19.544530591026245	26.958465494671476	22.92031962768277
4	33.50250608178914	25.302008314516332	23.431041429980514	17.764444173714004
5	28.966452640223917	31.379315599424277	26.516518837323964	13.137712923027841
6	24.965381514206	29.426264388774005	27.482710828049704	18.12564326897029
7	16.94994633067615	28.528248475056177	41.50429140240893	13.01751379185874
8	21.673438370248483	20.019568978173293	37.88308178591206	20.423910865666166
9	23.757430098835965	15.571695194886747	37.784543180771145	22.88633152550614
10-14	22.96361246489498	26.370985502257394	30.302806551417188	20.36259548143044
15-19	23.4439567425196	24.983356673612604	29.942164547978944	21.63052203588885
20-24	23.003860933078133	25.175642609751876	29.675391446209854	22.14510501096014
25-29	23.543920709299364	25.356633008946837	29.142663102678846	21.956783179074954
30-34	23.74180679311034	25.334632115496415	28.878403208803615	22.04515788258963
35-39	24.005756652657105	25.101622571232884	28.749898932215203	22.142721843894815
40-44	24.20737299692383	25.649332567437654	28.384440657056803	21.75885377858171
45-49	24.127560073432587	25.381948254627524	27.746946592571426	22.74354507936846
50-54	24.077716523381596	25.366098156349686	28.250880853903166	22.30530446636555
55-59	23.982724409193743	25.128133640771033	28.584099865879637	22.30504208415559
60-64	24.68948728161204	24.799106026367504	28.476066151847572	22.035340540172882
65-69	24.364405392477238	25.01906727701076	28.93503949737093	21.681487833141073
70-74	24.222075269623925	25.179783324224935	28.47347874593326	22.12466266021788
75-79	23.893406328156093	25.41917943530904	28.577513802057663	22.109900434477204
80-84	24.23768756048405	25.456443212138375	28.415537757037885	21.89033147033969
85-89	24.070128021105305	25.260305600296274	28.639672515301072	22.029893863297346
90-94	24.477723128451103	24.805176856629416	28.32940417336965	22.387695841549828
95-99	23.982269185973507	25.18521999080468	28.62768427986882	22.20482654335299
100-101	25.40438211093153	25.198765987571914	27.86088240895458	21.53596949254198
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	72.52915026342669
2	43.179728179035244
3	53.497003914302276
4	51.266950255503154
5	42.104165563251755
6	43.09102478317629
7	29.967460122534888
8	42.09734923591465
9	46.643761624342105
10-14	43.32620794632542
15-19	45.07447877840845
20-24	45.148965944038274
25-29	45.50070388837432
30-34	45.78696467569997
35-39	46.14847849655192
40-44	45.96622677550554
45-49	46.87110515280105
50-54	46.383020989747145
55-59	46.28776649334933
60-64	46.72482782178492
65-69	46.045893225618315
70-74	46.34673792984181
75-79	46.003306762633294
80-84	46.128019030823744
85-89	46.10002188440265
90-94	46.86541897000093
95-99	46.1870957293265
100-101	46.94035160347351
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	152.0
1	265.0
2	352.5
3	381.5
4	394.5
5	370.0
6	390.5
7	371.0
8	356.5
9	401.0
10	438.0
11	461.0
12	588.5
13	753.5
14	898.5
15	1176.0
16	1807.5
17	2772.5
18	4335.5
19	6833.5
20	10133.5
21	14677.5
22	21965.5
23	34727.0
24	49160.5
25	60088.5
26	73614.5
27	94866.0
28	120650.5
29	146090.0
30	178583.5
31	221632.5
32	268476.5
33	320996.0
34	378849.0
35	434680.0
36	483711.5
37	542959.5
38	597764.5
39	627184.5
40	636584.5
41	703624.5
42	795647.0
43	925149.0
44	1082763.5
45	1263729.0
46	1365016.5
47	1287355.5
48	1222922.5
49	1268397.5
50	1365652.0
51	1443141.0
52	1475411.5
53	1366076.0
54	1170750.5
55	1007752.5
56	880948.0
57	708527.0
58	466848.0
59	266858.5
60	142764.5
61	65257.0
62	26241.5
63	9593.5
64	4450.5
65	1974.5
66	778.0
67	261.0
68	89.0
69	53.5
70	35.0
71	24.0
72	20.0
73	14.5
74	6.5
75	6.0
76	5.5
77	3.0
78	3.0
79	1.5
80	2.0
81	1.5
82	0.0
83	0.5
84	0.5
85	1.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12146235436725679
2	1.2081540734726817E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	3.8972712047505865E-6
8	0.0
9	0.0
10-14	4.497450970282177E-4
15-19	1.7849502117757687E-4
20-24	7.794542409501173E-7
25-29	3.156789675847975E-4
30-34	2.72808984332541E-4
35-39	0.001780273486330068
40-44	5.144397990270775E-4
45-49	0.0015534523022135839
50-54	5.222343414365786E-5
55-59	0.0020803633690958633
60-64	0.002313420187139948
65-69	0.0014022381794692612
70-74	0.013798678427539929
75-79	0.01149461169129138
80-84	0.00219260477979268
85-89	0.0020351550231207565
90-94	0.004859897192323981
95-99	0.004436653539488068
100-101	0.0028391620726608024
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.5658979E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.78181509010118
#Duplication Level	Relative count
1	100.0
2	73.54246520072624
3	93.0603187411741
4	104.07504539035708
5	105.62840427678032
6	100.39338309461368
7	89.49969739761953
8	76.20536614888037
9	65.57393584829533
10++	1209.370587048618
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGATAATCTCGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGC	90950	0.3544568160720658	No Hit
GTAATCTTGAAGTGTGTTGCCGTCTTCAAGTTGCTTGCCGGCAAAGATCA	48999	0.190962391761574	No Hit
GTAAACGTTTGTCTTGATGTCACCAAGATCGTGGATAGCGACAACTTGGA	43179	0.1682802733499256	No Hit
GCGATAATCTCGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACG	39007	0.15202085788370612	No Hit
CTGAAGAATAACTGGAGACTCTGTCTTGACGGAAGCTGTGATGATAGCCT	36846	0.14359885481024012	No Hit
GGCGATAATCTCGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCAC	31973	0.1246074522294905	No Hit
GTTATTGCTACCCTCTTCCACCTGCTAAAATCGCAGGGAGTCCCAATTAG	28718	0.11192183445802734	No Hit
CTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGCACCACCAACGG	28076	0.10941978634457745	No Hit
CCTGGTTGCAGATGTACTTCTCGCCACCATAGTATAAGCCAGAGAGAGCT	27471	0.10706193726570336	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACCA	7213270	4.4866686	8.231708	50-54
CTTCT	12080380	4.183248	6.245006	6
CTTGA	9892165	3.583408	11.748986	1
CCTTG	8496315	3.5183504	4.554038	95-97
TCTTC	9474030	3.2807095	5.50335	7
CCTTC	7003615	3.223126	4.3112516	5
TTCTT	12099675	3.1527154	5.914797	6
CCACC	4333925	3.0816033	5.2433457	20-24
GCAGC	5265715	3.0315154	4.2084274	8
AGCCT	6280460	3.0235586	4.036589	9
GAAGA	6094240	2.6848178	5.7956977	3
TCTTG	8402735	2.6182265	5.3192067	7
CTGGA	5598950	2.4254203	14.04073	1
TGGAA	6353410	2.4075968	8.481573	2
CAGCC	3746220	2.3968573	5.7927885	9
CTTTG	7484740	2.3321865	5.2091665	1
CAACA	4171610	2.2698228	5.2461925	9
TGGAG	5730200	2.2335913	7.2791834	2
AAGAA	5133770	2.1987436	5.281314	4
CTGTG	5853305	2.181038	6.5193834	1
CCTGG	4259105	2.1091251	9.361251	1
TGAAG	5522535	2.0927403	5.82835	8
GGATG	5278875	2.0576682	5.413212	4
TCAAC	4310230	2.017299	5.1998844	8
CTGGG	4487340	1.9995234	17.281458	1
GCCTG	3976330	1.9690939	5.3164825	1
TGGGA	5036120	1.963044	11.035883	2
CTTGT	6042680	1.8828518	6.2139025	1
TTGAA	5858270	1.8563966	7.086212	7
TGGAT	5551610	1.8095785	5.103028	2
GTTGA	5241595	1.7085274	5.779912	1
CTGCT	4092940	1.6948992	5.03572	1
GATGT	5084855	1.6574371	5.533782	5
CTGGC	3326440	1.6472657	6.9543943	1
GTGGA	4180990	1.6297204	7.919337	1
CTGGT	4289120	1.5981967	10.781751	1
ATTTC	5157555	1.5623323	5.1350837	5
CTGAA	3615255	1.5225173	5.695645	1
GGCGA	2896810	1.500638	5.1070433	1
GATAA	3864955	1.42385	5.253694	2
GGAAT	3675435	1.3927901	5.77567	3
GGGAA	2994625	1.3570467	6.526839	3
GTTGG	3886805	1.3031917	5.9386873	1
AATCT	3691330	1.2999623	6.087386	5
CTGAG	2967690	1.2855796	5.2979283	1
GAATT	4029540	1.2768999	5.091948	4
GTGAA	3306585	1.2530158	6.1844807	1
TCTCG	3015115	1.2485685	5.14383	7
TGGTT	4063680	1.1393564	5.1582294	2
GTGGG	2737530	1.0976149	6.2295904	1
GGGAT	2691400	1.0490887	5.3290915	3
TGGGT	2974090	0.9971711	5.136582	2
ATCTC	2470110	0.99441516	5.1458383	6
CTCGC	1774055	0.9763321	6.8884172	8
>>END_MODULE