##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SDW_HiSeq_4_GCCAAT_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	22865788	
Filtered Sequences	0	
Sequence length	101	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97578303446179	34.0	31.0	34.0	31.0	34.0
2	33.136731609686926	34.0	33.0	34.0	31.0	34.0
3	33.18931392174195	34.0	33.0	34.0	31.0	34.0
4	36.52862591046502	37.0	37.0	37.0	35.0	37.0
5	36.392181585869686	37.0	37.0	37.0	35.0	37.0
6	36.384207839240005	37.0	37.0	37.0	35.0	37.0
7	36.365564309439065	37.0	37.0	37.0	35.0	37.0
8	36.386268341156665	37.0	37.0	37.0	35.0	37.0
9	38.22381017439679	39.0	39.0	39.0	37.0	39.0
10-14	38.4674265325997	39.4	39.0	39.4	37.2	39.4
15-19	39.69647584417383	41.0	40.0	41.0	37.6	41.0
20-24	39.633681848182974	41.0	40.0	41.0	37.0	41.0
25-29	39.48756033249324	41.0	39.2	41.0	37.0	41.0
30-34	39.32878678836697	40.4	39.0	41.0	36.4	41.0
35-39	39.15195659121829	40.0	39.0	41.0	36.0	41.0
40-44	38.9297333203649	40.0	38.2	41.0	35.0	41.0
45-49	38.70906819393235	40.0	38.0	41.0	35.0	41.0
50-54	38.53948983520708	40.0	37.8	41.0	34.8	41.0
55-59	37.99776261373542	39.4	36.6	41.0	34.0	41.0
60-64	37.425513085313305	38.6	35.4	40.6	33.8	41.0
65-69	36.549139063127846	36.8	35.0	39.6	33.0	41.0
70-74	35.65981783789827	35.6	35.0	38.0	32.2	40.4
75-79	34.594846624135585	35.0	34.2	36.2	31.4	39.0
80-84	34.508483232679325	35.0	34.8	35.8	32.0	37.0
85-89	34.12203773602729	35.0	34.0	35.0	32.0	36.2
90-94	33.882876251629725	35.0	34.0	35.0	31.4	36.0
95-99	33.71170888140833	35.0	34.0	35.0	31.0	35.0
100-101	33.241685657192306	35.0	33.5	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	5.0
13	41.0
14	196.0
15	628.0
16	1472.0
17	2680.0
18	4432.0
19	7078.0
20	10504.0
21	15029.0
22	21390.0
23	29804.0
24	40677.0
25	54549.0
26	72932.0
27	94076.0
28	122932.0
29	157875.0
30	203447.0
31	262495.0
32	349761.0
33	480607.0
34	709970.0
35	1241303.0
36	2943332.0
37	7486656.0
38	7165969.0
39	1384894.0
40	1050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.493745817883784	15.081360194739386	12.315714318366178	40.10917966901065
2	27.44796029312123	11.343816634836688	48.118402881933385	13.089820190108695
3	27.932122872826426	21.04400250715173	28.569787317191953	22.454087302829887
4	32.390167353952556	26.456604075923384	23.901957807008444	17.25127076311562
5	27.90590903755427	32.89186010121322	26.596411197374874	12.605819663857638
6	24.401625695121464	29.470766544323773	28.17549519832861	17.95211256222615
7	17.06147629812714	27.769521872589742	41.94279243733039	13.226209391952729
8	21.457637934892077	19.543699959082975	37.86103938337922	21.137622722645727
9	23.90648422000589	15.236439697595378	37.29972481158314	23.557351270815595
10-14	22.788746474872166	26.71043079922747	30.418108950947463	20.082713774952904
15-19	23.307965317978127	25.35675455334312	29.999221368162672	21.336058760516078
20-24	23.01253289018626	25.60391797925942	29.70913592688381	21.674413203670504
25-29	23.81894952582277	25.474061774240838	29.20709138643922	21.499897313497172
30-34	23.789597587397406	25.320951263563845	29.090177054568763	21.799274094469986
35-39	24.03754525554191	25.25777533231423	28.929823346358734	21.774856065785123
40-44	24.034819599629024	25.76529650103988	28.802508577748615	21.39737532158248
45-49	24.121548947291117	25.327610737831428	28.24376772676137	22.307072588116082
50-54	24.275205854381003	25.173214333008925	28.807072327029616	21.74450748558046
55-59	24.19759221970224	25.068306266045965	28.994991691655525	21.73910982259627
60-64	24.431897192163067	24.933432159301752	28.861010421178428	21.773660227356757
65-69	24.17628115341635	25.2329107244809	29.025749026770832	21.565059095331915
70-74	24.110280330190605	25.365215312585676	28.86049533279602	21.664009024427695
75-79	24.08568543142026	24.99864761117572	29.025956557911865	21.88971039949216
80-84	24.34477781820591	25.07540826070746	28.98009044880246	21.599723472284172
85-89	24.27169625213769	25.332938231389207	28.9551447566731	21.440220759800003
90-94	24.490128523209435	24.661441777583086	28.76404831490198	22.084381384305495
95-99	23.911796199792047	25.12433752685609	29.032952583729998	21.930913689621864
100-101	25.139200850621567	24.893694171071257	28.688145747666255	21.278959230640922
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	72.60292548689445
2	40.53778048322992
3	50.38621017565631
4	49.64143811706817
5	40.51172870141191
6	42.353738257347615
7	30.28768569007987
8	42.59526065753781
9	47.46383549082148
10-14	42.87146024982507
15-19	44.644024078494205
20-24	44.68694609385677
25-29	45.31884683931994
30-34	45.588871681867396
35-39	45.81240132132704
40-44	45.432194921211504
45-49	46.4286215354072
50-54	46.01971333996146
55-59	45.93670204229851
60-64	46.20555741951982
65-69	45.741340248748266
70-74	45.774289354618304
75-79	45.97539583091242
80-84	45.94450129049008
85-89	45.71191701193769
90-94	46.57450990751493
95-99	45.84270988941391
100-101	46.418160081262485
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	157.0
1	492.0
2	802.0
3	807.0
4	731.5
5	553.0
6	558.0
7	622.0
8	591.5
9	594.0
10	612.0
11	637.0
12	803.0
13	955.5
14	1007.0
15	1418.5
16	2145.0
17	3352.0
18	5234.0
19	7400.5
20	10872.0
21	18847.0
22	27274.5
23	36216.0
24	49201.5
25	62055.0
26	77567.0
27	99595.0
28	125316.0
29	154282.0
30	186195.5
31	232060.5
32	279423.0
33	315328.0
34	370704.5
35	422684.5
36	461875.0
37	505683.0
38	551254.0
39	580803.0
40	596656.0
41	652943.0
42	706718.0
43	775231.0
44	901772.0
45	1047403.5
46	1111203.5
47	1093236.0
48	1164910.5
49	1249286.0
50	1287913.0
51	1297832.0
52	1275607.5
53	1220878.0
54	1038406.5
55	841406.0
56	731335.0
57	576319.5
58	355282.0
59	185083.0
60	90730.0
61	41582.5
62	16482.0
63	5456.5
64	3182.0
65	1653.0
66	344.5
67	127.0
68	51.0
69	32.0
70	24.5
71	18.5
72	15.5
73	8.0
74	5.5
75	4.0
76	1.5
77	3.0
78	3.0
79	1.0
80	0.0
81	0.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.5
98	0.5
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12013581163264524
2	1.1808033906375762E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.040971603515261E-4
15-19	1.7143515893701104E-4
20-24	3.4986767130002255E-6
25-29	3.3237428773502144E-4
30-34	2.527793925142663E-4
35-39	0.0018000691688386161
40-44	5.003107699590322E-4
45-49	0.0015709058441371014
50-54	5.597882740800361E-5
55-59	0.0020231098092923804
60-64	0.002373852149770653
65-69	0.0013566118954658375
70-74	0.013302843532005107
75-79	0.011111797240488715
80-84	0.0022793878785196467
85-89	0.002036229846966131
90-94	0.004915640781765317
95-99	0.004591138516634546
100-101	0.0027377145279226765
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.2865788E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.21171321227578
#Duplication Level	Relative count
1	100.0
2	44.152479644707626
3	55.11966444608932
4	69.1339748334567
5	78.46039970392302
6	88.74907475943745
7	95.15173945225759
8	97.54502837404392
9	99.18578830495929
10++	1739.8100172711572
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTAAATTCGTCGAAGAATTTAGAAGCCACCACGAAGACGAAGAACGAGGT	44840	0.19610082976366264	No Hit
GTTGAGTCCTTTTGGATGGAGTAGTCTTGGAGTGTGTTGCCATCTTCAAG	38357	0.16774842835068707	No Hit
GGAGTGTTGAGTCCTTTTGGATGGAGTAGTCTTGGAGTGTGTTGCCATCT	37662	0.1647089529562681	No Hit
GTCCTTTTGGATGGAGTAGTCTTGGAGTGTGTTGCCATCTTCAAGCTGCT	36494	0.1596008849552878	No Hit
CTTGATTTCATTACCTTCTTTCTTAACGAAGAAAAGAGCTGGAATTGAAG	33014	0.14438164125373681	No Hit
CGATAATCTCGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGC	32177	0.14072115074276031	No Hit
GCCAGATCTTTTCCATATCATCCCAGTTGTTGACGATACCGTGTTCAATT	30049	0.1314146706861797	No Hit
CTTGTCGATACCATCGTATTCCATACCATCGAAGTATGGCTTGAAGAGCA	23193	0.10143101125576778	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CTTCT	11621100	4.503107	6.8078003	6
CACCA	5582455	4.0489993	5.3767085	65-69
CTTGA	8747050	3.5307727	13.372888	1
TCTTC	9096410	3.5248046	6.136094	7
CCTTG	7353865	3.4561617	4.1420093	45-49
CCTTC	6380365	3.372173	4.284629	10-14
TTCTT	11730775	3.332671	6.3564568	6
GAAGA	6564060	3.2333515	7.12907	2
GCAGC	4796425	3.2028637	4.726852	8
TCCTT	8072875	3.128191	4.3866377	2
CAACA	4254825	2.6505375	6.5881815	9
TCTTG	7612935	2.623199	5.237989	7
CAGCC	3311935	2.4870732	6.833236	9
TGGAG	5671280	2.468975	6.6163263	2
AAGAA	5162455	2.4561355	6.0091515	3
CTGGA	4944070	2.4205058	15.836832	1
TGGAA	5711080	2.401428	8.556718	2
CTTTG	6896285	2.376262	6.731572	1
TGTTG	7658130	2.3464746	5.0476046	5
TCAGC	4048615	2.2290199	5.3508472	8
CCTGG	3888550	2.2165596	8.331518	1
CTGTG	5095560	2.129533	6.2329535	1
CTGGG	4085230	2.0707245	16.55751	1
TCAAC	3852330	2.0485504	5.9230423	8
TGAAG	4826640	2.0295334	5.0602927	2
GTTGA	5331280	1.9136112	8.062142	1
TTGAT	6371155	1.8855021	5.3185	2
CTTGG	4497180	1.8794583	5.0795817	1
TGGGA	4311535	1.877014	9.823781	2
TTGAG	5229165	1.876958	5.8998675	2
CTTGT	5404885	1.8623681	6.6020794	1
TTGAA	5330665	1.8480738	5.436855	2
TGGAT	4999260	1.794436	5.33796	2
GATCT	4397905	1.7752275	5.213404	5
CTGGC	2996110	1.707849	7.910555	1
GATGT	4551290	1.6336415	5.1584826	5
GTGGA	3653495	1.5905383	6.371544	1
CTGGT	3753655	1.5687249	9.696314	1
ATTTC	4574240	1.5223473	5.671121	5
CTCTG	3130685	1.471356	5.2119126	1
GGGAA	2542800	1.2968116	5.669757	3
GTGAA	3070665	1.2911711	5.5082784	1
GATTT	4331720	1.2819448	5.123303	4
GTTGG	3392890	1.2608873	5.5446935	1
CTGAA	2576215	1.218202	5.535273	1
GTAAA	2881795	1.1703887	6.074899	1
TTTCA	3423885	1.1394991	5.775224	6
>>END_MODULE