##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SDW_HiSeq_3_ACAGTG_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	41540808	
Filtered Sequences	0	
Sequence length	101	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00187620327462	34.0	33.0	34.0	31.0	34.0
2	33.16526524953487	34.0	33.0	34.0	31.0	34.0
3	33.218321896868254	34.0	34.0	34.0	31.0	34.0
4	36.54186863673908	37.0	37.0	37.0	35.0	37.0
5	36.41875863367896	37.0	37.0	37.0	35.0	37.0
6	36.409607632090356	37.0	37.0	37.0	35.0	37.0
7	36.397778324388874	37.0	37.0	37.0	35.0	37.0
8	36.4178758391026	37.0	37.0	37.0	35.0	37.0
9	38.256409528673586	39.0	39.0	39.0	37.0	39.0
10-14	38.504246166805416	39.4	39.2	39.4	37.2	39.4
15-19	39.74642922207965	41.0	40.0	41.0	38.0	41.0
20-24	39.68643314304334	41.0	40.0	41.0	37.4	41.0
25-29	39.55113134053627	41.0	39.8	41.0	37.0	41.0
30-34	39.392854231434306	40.8	39.0	41.0	36.8	41.0
35-39	39.214256265790496	40.0	39.0	41.0	36.0	41.0
40-44	38.99702933077277	40.0	38.4	41.0	35.4	41.0
45-49	38.770234093665195	40.0	38.0	41.0	35.0	41.0
50-54	38.60550487607271	40.0	37.8	41.0	35.0	41.0
55-59	38.05151095279611	39.4	36.6	41.0	34.0	41.0
60-64	37.45755665609585	38.6	35.4	40.6	34.0	41.0
65-69	36.58034187491009	36.8	35.0	39.6	33.0	41.0
70-74	35.7036318552109	35.6	35.0	38.2	32.6	40.4
75-79	34.659256261938864	35.0	34.2	36.2	31.4	39.0
80-84	34.56368046090967	35.0	35.0	35.8	32.0	37.2
85-89	34.17734967504724	35.0	34.2	35.0	32.0	36.2
90-94	33.94045315151309	35.0	34.0	35.0	32.0	36.0
95-99	33.767303341812706	35.0	34.0	35.0	31.0	35.0
100-101	33.28301326011762	35.0	33.5	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	7.0
12	21.0
13	84.0
14	371.0
15	1120.0
16	2506.0
17	4747.0
18	7756.0
19	12198.0
20	18441.0
21	25797.0
22	36247.0
23	50539.0
24	69665.0
25	93880.0
26	124762.0
27	162615.0
28	210988.0
29	271848.0
30	350050.0
31	451780.0
32	601892.0
33	831871.0
34	1238059.0
35	2190031.0
36	5244527.0
37	1.3665277E7
38	1.3231734E7
39	2634393.0
40	7596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.1023653807474	14.017230618386357	15.348998415623802	36.53140558524244
2	30.003531469558347	11.852654187042228	43.609686674154894	14.534127669244533
3	29.69993024690324	21.418562681785104	26.541229048794623	22.34027802251704
4	32.641762769756426	26.99006480567253	22.963294310500654	17.404878114070385
5	28.2714577915769	33.00013085927457	25.893882468535516	12.83452888061301
6	24.56373982903751	29.60975145211427	27.515016077684386	18.31149264116384
7	17.238619995829644	27.735668175111307	41.5671777352782	13.458534093780857
8	22.192108059140303	20.01392462082105	37.11093197802026	20.683035342018382
9	24.012869465610777	15.551450034385464	36.64098204348842	23.794698456515338
10-14	22.927112134507716	26.762426711560362	30.207521342036276	20.102939811895652
15-19	23.355884132130463	25.316356613945963	29.768218089373537	21.55954116455004
20-24	22.95389324481725	25.50321735928931	29.51846871456752	22.024420681325914
25-29	23.67847078360453	25.615765550888852	28.96703537219737	21.738728293309244
30-34	23.642315927035238	25.57326762462988	28.858293559907978	21.926122888426907
35-39	24.016871046164514	25.39342038251091	28.76060699406096	21.829101577263614
40-44	24.277147290493915	25.618730682416135	28.64591813243038	21.45820389465957
45-49	24.194490430388523	25.29176536625617	28.319136363548168	22.19460783980714
50-54	24.426370542037827	25.246561211641	28.522902210901684	21.804166035419488
55-59	24.240155158309953	25.106761034940234	28.721770763870534	21.931313042879278
60-64	24.51639555010232	24.942070730843945	28.811162469947256	21.730371249106483
65-69	24.330834203434453	25.135765023324037	28.826544875539934	21.706855897701576
70-74	24.172329136065404	25.43829078814853	28.52697047533242	21.862409600453645
75-79	24.09660918942935	25.029607135728032	28.784220298045167	22.08956337679745
80-84	24.378785343872742	25.29165137472492	28.57073333544504	21.758829945957295
85-89	24.224499836029967	25.351054104660808	28.653131277803475	21.771314781505748
90-94	24.717679566549975	24.580247798668434	28.40530096231656	22.29677167246503
95-99	24.189003727909338	25.00680794945862	28.698087558778646	22.106100763853398
100-101	25.566536381046134	24.7505457529155	28.099970025010933	21.58294784102743
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	70.63377096598984
2	44.53765913880288
3	52.040208269420276
4	50.04664088382681
5	41.10598667218991
6	42.875232470201354
7	30.6971540896105
8	42.87514340115869
9	47.80756792212612
10-14	43.03005194640336
15-19	44.9154252966805
20-24	44.978313926143166
25-29	45.417199076913775
30-34	45.568438815462144
35-39	45.84597262342813
40-44	45.73535118515348
45-49	46.38909827019566
50-54	46.230536577457315
55-59	46.17146820118923
60-64	46.24676679920881
65-69	46.03769010113603
70-74	46.03473873651905
75-79	46.1861725662268
80-84	46.137615289830045
85-89	45.995814617535714
90-94	47.01445123901501
95-99	46.295104491762736
100-101	47.14948422207356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	450.0
1	1027.0
2	1495.5
3	1457.0
4	1306.0
5	1111.0
6	1258.5
7	1283.5
8	1171.0
9	1196.0
10	1194.0
11	1242.0
12	1677.0
13	1899.0
14	1994.0
15	2739.0
16	3858.5
17	5753.5
18	8751.0
19	13280.0
20	20655.0
21	30053.0
22	46990.5
23	79528.5
24	105646.0
25	120062.5
26	141778.5
27	181920.0
28	223117.5
29	267275.5
30	333053.0
31	408099.5
32	483210.5
33	565804.5
34	657684.5
35	732915.0
36	824135.0
37	945682.0
38	1016734.5
39	1019406.0
40	1033873.0
41	1190152.0
42	1267423.0
43	1408594.0
44	1663489.5
45	1822124.5
46	1919019.5
47	1895081.0
48	1898572.5
49	1956316.5
50	2214470.5
51	2366249.0
52	2340170.5
53	2258018.0
54	1982327.0
55	1709844.0
56	1528794.0
57	1219636.5
58	766852.5
59	430854.0
60	222795.0
61	106113.0
62	42987.0
63	18710.0
64	12039.0
65	6382.5
66	1361.0
67	399.5
68	153.0
69	85.0
70	58.0
71	45.5
72	37.5
73	27.5
74	15.0
75	13.0
76	12.5
77	9.0
78	8.5
79	7.0
80	3.5
81	4.0
82	3.0
83	0.5
84	1.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	0.5
92	1.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12347617311632457
2	6.740359985294461E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	1.4443628539916702E-5
8	0.0
9	0.0
10-14	4.4341939617544273E-4
15-19	1.8535989959559765E-4
20-24	3.37017999264723E-6
25-29	3.8516342773111203E-4
30-34	3.1631546502417575E-4
35-39	0.0018391553674160601
40-44	5.348957102615818E-4
45-49	0.0016836456334696232
50-54	6.78850541376085E-5
55-59	0.0021342868439150242
60-64	0.0024371215889686113
65-69	0.0014852864681881006
70-74	0.014052206206484959
75-79	0.011735929642966983
80-84	0.0023008700264087302
85-89	0.0021679886438414966
90-94	0.005021568189044373
95-99	0.004498227381614725
100-101	0.0028321548295353327
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4.1540808E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	97.57195846898942
#Duplication Level	Relative count
1	100.0
2	86.81978461831697
3	99.56161250357381
4	100.3621461926999
5	89.19279519679786
6	74.54493471838369
7	62.04136090727152
8	51.18650528924044
9	42.838082531211285
10++	1199.4853711998476
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTGATTTCATTACCTTCTTTCTTAACGAAGAAAAGAGCTGGAATTGAAG	123703	0.2977866968788859	No Hit
CTGGAATTGAAGAGACTCCGTAAGCATCTGCAGCATTGCCGTTCTTATCA	57171	0.1376261145425963	No Hit
GGCTGATCCTCGAGGAATTCCTTCTCATCATCCTCAGCAGCAGCGACCTT	50952	0.12265529356097263	No Hit
AACAATTTCACCGTTTTCCATTCTCTTGATTAATTCGAGAGCCTGAGCAA	47290	0.11383986560877679	No Hit
TTTGTCTTCTGGAGGACAACAGCCTGCTTGCCCTTCATGGCGAAGACCAT	46662	0.11232809915493218	No Hit
TAACAATTTCACCGTTTTCCATTCTCTTGATTAATTCGAGAGCCTGAGCA	43443	0.10457909244326687	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CTTCT	21177935	4.604292	7.3714085	6
CACCA	10478965	4.1048126	5.464513	65-69
CTTGA	16245065	3.63636	12.726742	1
CCTTG	13774925	3.5750408	5.087554	95-97
CCTTC	11917280	3.4795187	4.804915	10-14
TCTTC	15942060	3.465961	5.7432866	7
TTCTT	21336540	3.4541442	6.291849	6
GCAGC	9262765	3.3240027	4.7946415	35-39
AGCCT	10243835	3.0794332	3.859466	9
TCCTT	14040085	3.052453	3.885038	40-44
GAAGA	11310695	3.0193768	5.4228144	3
CAACA	7855225	2.6539164	6.5187426	9
TCTTG	13291770	2.5686905	5.0846443	7
CTGGA	9246680	2.470835	14.371094	1
CTTTG	12614060	2.43772	6.066263	1
CAGCC	6017085	2.4291656	6.9236636	9
TGGAA	10440810	2.406278	8.201559	2
CCTGG	7571875	2.34589	7.818574	1
GATGA	10057080	2.3178399	5.1066933	6
CTGTG	9621125	2.2195623	5.3122296	1
TTCTG	11377630	2.1987748	5.35719	7
CTGGG	7797735	2.1474469	14.49173	1
TGGAG	8918000	2.1182399	6.1714196	2
GGATG	8523310	2.0244913	5.212232	4
TGGGA	8289695	1.9690021	9.902538	2
TCAAC	6673055	1.9464211	5.1029615	8
GAGAA	7160750	1.9115539	5.113665	2
TTGAT	11340005	1.8901482	5.321249	2
TTGAA	9668570	1.8666433	5.9874506	7
CTTGT	9582435	1.8518459	5.0068626	1
TTCAT	8951035	1.6784413	5.911653	7
GATGT	8402025	1.6717799	5.8132095	5
GTTGA	8328300	1.6571107	5.3911586	1
CTGGC	5306425	1.6440169	6.8870964	1
CTGGT	6817425	1.5727576	8.369472	1
ATTTC	8323385	1.5607485	7.4099	5
GGAAA	5824415	1.5548208	5.149654	3
GAAAG	5684545	1.5174826	5.5446362	4
GGAAT	6259960	1.4427236	6.1107087	3
GTGGA	6055995	1.4384446	6.1087193	1
GGGAA	5086940	1.399525	6.156544	3
GTGAA	6024045	1.3883528	5.6472764	1
GAATT	6985335	1.3486098	5.362268	4
GATTT	7667655	1.2780421	6.0096235	4
CTGAA	4728545	1.2259969	5.0742135	1
TTTCA	6054545	1.1353097	7.0888915	6
GTAAA	4989055	1.1156652	5.1571503	1
GTGGG	4513865	1.1049746	5.46394	1
>>END_MODULE