##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SDW_HiSeq_2_TGACCA_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	26577734	
Filtered Sequences	0	
Sequence length	101	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.986907913217884	34.0	31.0	34.0	31.0	34.0
2	33.11494294434582	34.0	33.0	34.0	31.0	34.0
3	33.19369107990922	34.0	33.0	34.0	31.0	34.0
4	36.542268690024514	37.0	37.0	37.0	35.0	37.0
5	36.420705354339084	37.0	37.0	37.0	35.0	37.0
6	36.412457171856715	37.0	37.0	37.0	35.0	37.0
7	36.390455597155125	37.0	37.0	37.0	35.0	37.0
8	36.410100989045944	37.0	37.0	37.0	35.0	37.0
9	38.253824046850646	39.0	39.0	39.0	37.0	39.0
10-14	38.491476970911066	39.4	39.2	39.4	37.2	39.4
15-19	39.73012214660587	41.0	40.0	41.0	37.8	41.0
20-24	39.672232689212706	41.0	40.0	41.0	37.4	41.0
25-29	39.54152080835785	41.0	39.8	41.0	37.0	41.0
30-34	39.37480886068015	40.8	39.0	41.0	36.8	41.0
35-39	39.20419917664914	40.0	39.0	41.0	36.0	41.0
40-44	38.9414159386199	40.0	38.2	41.0	35.2	41.0
45-49	38.736581990022174	40.0	38.0	41.0	35.0	41.0
50-54	38.589421701639424	40.0	37.8	41.0	34.8	41.0
55-59	38.061702649292826	39.6	36.6	41.0	34.0	41.0
60-64	37.47824719744731	38.8	35.4	40.6	33.8	41.0
65-69	36.58437814149242	36.8	35.0	39.6	33.0	41.0
70-74	35.518759620364925	35.6	35.0	38.4	32.0	40.4
75-79	34.41012389543819	35.0	34.2	36.2	30.8	39.0
80-84	34.30221014327255	35.0	35.0	35.8	32.0	37.2
85-89	33.904959264021535	35.0	34.0	35.0	31.2	36.2
90-94	33.65522351905547	35.0	34.0	35.0	31.0	36.0
95-99	33.47919003930132	35.0	34.0	35.0	31.0	35.0
100-101	33.00240821884965	35.0	33.5	35.0	29.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	13.0
13	47.0
14	191.0
15	662.0
16	1586.0
17	3081.0
18	5213.0
19	8569.0
20	13150.0
21	19510.0
22	27891.0
23	39689.0
24	54492.0
25	73662.0
26	98766.0
27	132222.0
28	180896.0
29	251865.0
30	310434.0
31	307332.0
32	381973.0
33	526260.0
34	777840.0
35	1366273.0
36	3248298.0
37	8512913.0
38	8490773.0
39	1742329.0
40	1798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.41462199511233	15.792997717168566	12.390761793797173	33.40161849392194
2	25.897140170465445	14.07899696975318	45.78461878364733	14.239244076134044
3	26.419517179305053	22.86159534894886	29.297106367307315	21.421781104438775
4	31.232941077670507	27.665266722889164	22.853739148717494	18.248053050722834
5	28.23999593042808	32.60474726701682	25.869500387053314	13.285756415501787
6	24.39824629142575	29.817263578603054	27.641938925267297	18.142551204703906
7	16.187883615342617	28.588400890519992	41.90063635983961	13.323079134297775
8	20.830707388372538	20.26578714347882	36.96984099547388	21.933664472674756
9	24.272152020183512	15.703667588817016	36.67907504830924	23.34510534269024
10-14	22.467951690616587	27.006487528167977	29.98440755265776	20.541153228557675
15-19	22.87355918510496	25.461755973678578	30.055599198265774	21.609085642950685
20-24	22.586806443020457	26.04684039760892	29.738519387812058	21.627833771558564
25-29	23.260902669215337	25.75181773862948	29.358521936997985	21.6287576551572
30-34	23.159862768665377	25.654200325477845	29.54036686553066	21.64557004032612
35-39	23.578363712960925	25.837431029455516	28.76422879455473	21.81997646302883
40-44	23.552075527807233	25.829742419773975	29.18598108931722	21.432200963101568
45-49	24.060384778074095	25.689096865918703	28.617397241419688	21.63312111458751
50-54	23.855806761557233	25.353723185366956	28.672477664730117	22.1179923883457
55-59	23.726692764147593	25.315831528764853	29.46228191380878	21.495193793278773
60-64	24.221012326919524	25.13777517851438	29.20605392411818	21.43515857044791
65-69	23.674370999834522	26.39301422888929	28.717140143982817	21.215474627293375
70-74	23.561141418354296	26.335091764642947	28.619594907706798	21.48417190929596
75-79	23.535905286234037	25.983428914138784	28.872037871176975	21.608627928450204
80-84	23.759505765798785	25.884720717503434	28.806000472707122	21.54977304399066
85-89	23.655015204544686	25.855428813432056	28.970764744492207	21.518791237531055
90-94	24.0130298627951	25.372065813270794	28.64242564553598	21.972478678398126
95-99	23.5713728133629	25.608012464461062	28.96427550899548	21.856339213180558
100-101	24.809199841878446	25.541710429407775	28.206226416187636	21.442863312526136
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	71.81624048903426
2	40.13638424659949
3	47.841298283743825
4	49.48099412839334
5	41.52575234592987
6	42.540797496129656
7	29.510962749640395
8	42.764371861047294
9	47.61725736287375
10-14	43.00910491917426
15-19	44.48264482805565
20-24	44.21464021457902
25-29	44.889660324372535
30-34	44.8054328089915
35-39	45.39834017598975
40-44	44.984276490908805
45-49	45.693505892661605
50-54	45.97379914990293
55-59	45.221886557426366
60-64	45.65617089736743
65-69	44.8898456271279
70-74	45.045313327650256
75-79	45.14453321468424
80-84	45.30927880978945
85-89	45.17380644207574
90-94	45.98550854119323
95-99	45.42771202654346
100-101	46.25206315440458
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	283.0
1	658.0
2	1023.0
3	1045.5
4	994.5
5	881.5
6	909.5
7	971.0
8	923.0
9	907.0
10	924.0
11	980.5
12	1333.0
13	1532.5
14	1638.5
15	2125.0
16	3083.0
17	4787.5
18	6992.0
19	9848.0
20	15288.5
21	23339.0
22	34681.5
23	51886.5
24	72485.5
25	91543.5
26	109593.5
27	136560.5
28	167557.0
29	203272.0
30	247339.5
31	294921.5
32	344688.5
33	398907.0
34	472996.0
35	530470.5
36	575332.5
37	637390.5
38	689486.5
39	717433.0
40	727268.5
41	780993.5
42	844814.0
43	944358.5
44	1095639.0
45	1280636.5
46	1342722.5
47	1262053.0
48	1244356.5
49	1305362.0
50	1433853.0
51	1501174.5
52	1459166.0
53	1334604.5
54	1137886.0
55	942722.0
56	785257.5
57	585269.0
58	357921.5
59	194043.0
60	93117.0
61	41139.5
62	16141.5
63	5346.0
64	2808.0
65	1494.5
66	385.0
67	132.5
68	66.5
69	32.5
70	20.5
71	17.0
72	14.5
73	13.5
74	5.5
75	3.0
76	2.5
77	2.0
78	3.0
79	3.5
80	2.0
81	1.0
82	0.5
83	2.0
84	3.0
85	1.5
86	0.5
87	0.0
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12112018278157197
2	1.1663898810937005E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5050192014112264E-5
8	0.0
9	0.0
10-14	4.6279340443395207E-4
15-19	1.8285983297146403E-4
20-24	2.25752880211684E-6
25-29	3.596995891372831E-4
30-34	2.7165596585472637E-4
35-39	0.0018158056665026445
40-44	5.365393453031022E-4
45-49	0.0016555211215523492
50-54	6.0953277657154664E-5
55-59	0.002101006805170072
60-64	0.002377177828629032
65-69	0.0014899690093971142
70-74	0.013664821839213229
75-79	0.011332794586626536
80-84	0.002262043859721073
85-89	0.0020648863443362025
90-94	0.004916897731010477
95-99	0.004446579230569469
100-101	0.002678934178511983
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.6577734E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	96.39037639469745
#Duplication Level	Relative count
1	100.0
2	98.75302835969788
3	102.52244548952544
4	92.64643009833262
5	75.73036910360553
6	59.77625765996865
7	48.517885136098045
8	38.93401738634744
9	33.64685763146644
10++	774.5831551945275
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC	310526	1.1683689813435563	TruSeq Adapter, Index 4 (100% over 50bp)
CGATAATCTCGCTCGTTAGCTGAATCAACGCTAGACAGGTCAACCCACGC	45687	0.17189953063718677	No Hit
GTTATTGCTACCCTCTTCCACCTGCTAAAATCGCAGGGAGTCCCAATTAG	37732	0.141968461269121	No Hit
GCCAGATCTTTTCCATATCATCCCAGTTGTTGACGATACCGTGTTCAATT	37543	0.14125733969645418	No Hit
GTAATCTTGAAGTGTGTTGCCGTCTTCAAGTTGCTTGCCGGCAAAGATCA	37308	0.14037314091562508	No Hit
CTTGATTTCATTACCTTCTTTCTTAACGAAGAAAAGAGCTGGAATTGAAG	29538	0.111138142928212	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CTTCT	13170560	4.4492507	6.6037774	6
CACCA	6368980	3.8618546	5.689292	65-69
TCTTC	10582535	3.5749693	6.242661	7
CTTGA	10139055	3.5306704	10.774664	1
TTCTT	13417880	3.3127997	6.18093	6
CCTTG	7921860	3.2955074	4.4681687	95-97
CCTTC	6914355	3.1959925	4.50218	10-14
GAAGA	6980455	2.869832	16.820452	6
TCTTG	8524615	2.591782	5.6559753	7
CAACA	4834380	2.4537563	5.743269	9
AAAAA	6777710	2.4104767	11.028885	65-69
CTTTG	7702965	2.3419716	5.4504375	1
CTGGA	5428755	2.327943	11.210773	1
CAGCC	3570180	2.3275154	5.9240537	9
AGAGC	4738100	2.3270855	19.18173	8
TTCTG	7592865	2.3084974	5.072817	7
GGAAG	5186200	2.2924423	16.850922	5
TGGAA	6370915	2.2868547	6.9580865	2
CCAGA	3948765	2.154906	5.1241913	2
GAGCA	4364740	2.1437123	19.723614	9
TGGAG	5344125	2.0624802	5.627446	2
CCTGG	4002745	2.0505266	7.271102	1
CTGGG	4290975	1.9783564	12.034439	1
TTGAA	6671095	1.9445605	6.000648	7
TCAAC	4262955	1.8891456	5.365194	8
GCCAG	3126190	1.834251	5.4488115	1
CTTGT	6009875	1.8272128	5.316985	1
CTGGC	3418290	1.751122	6.0279107	1
TGGGA	4521000	1.7448082	7.947338	2
AGCAC	3186760	1.7390676	5.6671796	10-14
GTTGA	5421440	1.6990862	7.721516	1
GATCT	4856140	1.6910284	5.404532	5
AAGAG	4075420	1.6755025	15.6769285	7
GATGT	5042355	1.5802807	5.246161	5
ATTTC	5471195	1.5471395	5.3942885	5
CTGGT	4120590	1.5427499	7.3534117	1
GTGGA	3800315	1.4666712	6.67747	1
GTTGG	3861870	1.3012922	7.109043	1
GTGAA	3455140	1.2402306	6.014553	1
CGGAA	2444385	1.2005429	17.376606	4
TTTCA	4008605	1.1335497	5.1839204	6
GTAAA	3364195	1.1231596	5.034474	1
GTGGG	2581700	1.0712612	5.098356	1
GATCG	2031850	0.87129205	14.720607	1
TCGGA	1744740	0.74817437	15.587435	3
ATCGG	1466445	0.6288367	14.655577	2
>>END_MODULE