##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SDW_HiSeq_1_CGATGT_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	19965718	
Filtered Sequences	0	
Sequence length	101	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97028812086798	34.0	31.0	34.0	31.0	34.0
2	33.04763590270082	34.0	33.0	34.0	31.0	34.0
3	33.16802636399051	34.0	33.0	34.0	31.0	34.0
4	36.53517940101128	37.0	37.0	37.0	35.0	37.0
5	36.41712985227979	37.0	37.0	37.0	35.0	37.0
6	36.40437258504803	37.0	37.0	37.0	35.0	37.0
7	36.39816900148545	37.0	37.0	37.0	35.0	37.0
8	36.42228879522389	37.0	37.0	37.0	35.0	37.0
9	38.2751167776686	39.0	39.0	39.0	37.0	39.0
10-14	38.51665079112106	39.4	39.2	39.4	37.2	39.4
15-19	39.75967806416979	41.0	40.0	41.0	38.0	41.0
20-24	39.69575181819157	41.0	40.0	41.0	37.6	41.0
25-29	39.53850356896756	41.0	39.8	41.0	37.0	41.0
30-34	39.37560791953488	41.0	39.0	41.0	36.8	41.0
35-39	39.19717941523565	40.0	39.0	41.0	36.0	41.0
40-44	38.97539720835484	40.0	38.6	41.0	35.2	41.0
45-49	38.722761405324874	40.0	38.0	41.0	35.0	41.0
50-54	38.56117862628331	40.0	37.8	41.0	35.0	41.0
55-59	38.02597994221895	39.6	36.6	41.0	34.0	41.0
60-64	37.46948570544771	38.8	35.4	40.8	33.8	41.0
65-69	36.6341948233467	36.8	35.0	39.8	33.0	41.0
70-74	35.778148784832084	35.6	35.0	38.8	32.6	40.8
75-79	34.7290084033041	35.0	34.2	36.8	31.4	39.2
80-84	34.65784684527749	35.0	35.0	36.0	32.0	38.0
85-89	34.230234374741734	35.0	34.4	35.2	32.0	36.6
90-94	33.920814828697864	35.0	34.0	35.0	31.4	36.0
95-99	33.70023739692206	35.0	34.0	35.0	31.0	35.8
100-101	33.20038553083841	35.0	33.5	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	6.0
11	10.0
12	35.0
13	118.0
14	336.0
15	1171.0
16	2584.0
17	4534.0
18	6590.0
19	9841.0
20	12723.0
21	16309.0
22	21448.0
23	27953.0
24	36728.0
25	48833.0
26	63986.0
27	83524.0
28	109194.0
29	144523.0
30	181199.0
31	218541.0
32	288343.0
33	396023.0
34	588482.0
35	1021763.0
36	2499136.0
37	6268834.0
38	6163391.0
39	1741355.0
40	8204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	57.104087133666795	13.16789667008468	5.99552950323267	23.732486693015858
2	25.654686635783364	10.487096944324058	45.42793572395695	18.430280695935632
3	26.896934034628757	23.095317684042215	28.771973038986125	21.2357752423429
4	31.448015042584494	24.8519637510657	24.58023297734647	19.119788229003333
5	28.13081402832595	33.10667314844375	25.709593814757874	13.052919008472422
6	23.777211518263456	30.609147139111148	26.37647191050179	19.2371694321236
7	16.569522647245776	28.421032913568794	41.34215665783503	13.667287781350401
8	18.319912161435916	22.835772798153315	37.53409719600367	21.3102178444071
9	22.75323632238019	16.12034688659832	36.64271928512663	24.483697505894856
10-14	21.284972502343628	26.770967513930742	32.186907131119256	19.757152852606374
15-19	22.331885388863334	24.90963845223465	31.606770793734135	21.151705365167878
20-24	21.899556248360877	25.29169574859963	30.93697237371551	21.87177562932398
25-29	22.836988014846817	25.78391503784685	30.171102549808015	21.207994397498318
30-34	22.55843463694641	24.84005235710133	30.54053854792988	22.060974458022383
35-39	22.811491872612876	24.212906206323726	31.186632118026345	21.788969803037048
40-44	23.107352471103567	25.096923139954523	30.05838738543177	21.737337003510135
45-49	23.354837384803748	24.33391592498717	29.884194068884725	22.427052621324357
50-54	23.106020969916276	23.847645604011376	30.319495626370713	22.726837799701634
55-59	23.112283443424726	23.97050084539715	30.306301373782148	22.610914337395975
60-64	22.904885752878222	23.92092171482332	30.36689139025649	22.807301142041972
65-69	22.74608216050702	24.561098577995153	30.18954895668296	22.50327030481487
70-74	22.987390095536064	24.191965101152462	30.400665143366613	22.419979659944865
75-79	23.023515060332578	23.514935103473103	30.593893917043353	22.867655919150966
80-84	22.897013275777944	23.852141464274712	30.5148655529378	22.735979707009545
85-89	23.462815504322325	24.34820078058443	29.90902364237306	22.279960072720183
90-94	23.8899796895956	24.241094656125497	29.34203890670708	22.526886747571826
95-99	23.982161508872736	24.784389322750776	29.319371633341827	21.91407753503466
100-101	24.889617157076906	24.826965557147076	29.714436476819824	20.568980808956198
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	80.83657382668264
2	44.084967331719
3	48.13270927697166
4	50.56780327158783
5	41.18373303679837
6	43.01438095038706
7	30.23681042859618
8	39.630130005843014
9	47.23693382827505
10-14	41.04212535495
15-19	43.48359075403121
20-24	43.77133187768486
25-29	44.044982412345135
30-34	44.61940909496879
35-39	44.600461675649925
40-44	44.8446894746137
45-49	45.78189000612811
50-54	45.832858769617914
55-59	45.7231977808207
60-64	45.712186894920194
65-69	45.249352465321884
70-74	45.40736975548093
75-79	45.89117097948355
80-84	45.63299298278749
85-89	45.74277557704251
90-94	46.41686643716743
95-99	45.89623904390739
100-101	45.458597966033096
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3935.0
1	3157.5
2	2304.0
3	2197.0
4	2128.0
5	2047.5
6	2024.5
7	1921.5
8	1841.0
9	1846.0
10	1779.0
11	1881.5
12	2232.0
13	2210.5
14	2155.5
15	2331.5
16	2547.0
17	3293.5
18	4262.5
19	6148.5
20	9884.0
21	13939.0
22	21100.5
23	55647.5
24	78037.5
25	74193.5
26	92741.5
27	121247.5
28	119532.0
29	121439.0
30	157967.5
31	216158.0
32	295465.5
33	413723.5
34	537457.0
35	505702.0
36	435473.5
37	430495.5
38	474057.0
39	563150.5
40	552565.0
41	540220.5
42	577100.0
43	622040.5
44	715357.5
45	815251.0
46	861491.0
47	870550.0
48	861681.0
49	887661.5
50	969925.5
51	1051591.0
52	1114222.0
53	1111566.5
54	993505.0
55	776986.5
56	603185.5
57	469767.5
58	330892.5
59	218166.5
60	120559.0
61	56494.5
62	27165.0
63	16365.5
64	8987.5
65	3016.0
66	1122.0
67	360.0
68	79.0
69	45.5
70	32.0
71	23.5
72	16.5
73	12.0
74	9.0
75	4.0
76	2.0
77	0.5
78	0.0
79	0.0
80	2.0
81	2.0
82	0.5
83	0.5
84	0.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12175870659898132
2	1.0518028953429074E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	5.008585215918606E-6
8	0.0
9	0.0
10-14	4.4676580125993965E-4
15-19	1.7630219960033494E-4
20-24	1.0017170431837212E-6
25-29	3.295649072074443E-4
30-34	2.985116788687489E-4
35-39	0.0017990838095579633
40-44	5.208928624555351E-4
45-49	0.0016678588769008958
50-54	5.609615441828839E-5
55-59	0.0021396676042404286
60-64	0.0024031191865977472
65-69	0.0015065824329483168
70-74	0.013741554398394288
75-79	0.011557811244253775
80-84	0.002202775777961003
85-89	0.0021086143759017333
90-94	0.004884372302563825
95-99	0.004459644276253927
100-101	0.0027321832352835995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.9965718E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	98.54107878706441
#Duplication Level	Relative count
1	100.0
2	21.414180178451502
3	17.039240141993666
4	18.737407656145063
5	22.268061018900507
6	26.31679938597333
7	30.682145255684542
8	34.71169528926413
9	39.23054782692123
10++	1608.4332725702773
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGAGTTTCTTCTTTCTTTGGCTTATCTTCTGTCTTCTTATCATCTTTCT	127713	0.639661443680613	No Hit
GTGCTGTATACCATCGGAGCCACTATACATTGATGATGTGAATGAGTAGT	114093	0.5714445130398016	No Hit
GCACAAAGTTTTTTTCTTTCCTTAGTGAGTTTCTTCTTTCTTTGGCTTAT	110024	0.5510645797962287	No Hit
GTTTAGTTTAAAGATACTTCTCAAGGCGGTGGAAGATATCAGCGCAGCGG	51094	0.25590865302214527	No Hit
CAGCACAAAGTTTTTTTCTTTCCTTAGTGAGTTTCTTCTTTCTTTGGCTT	50869	0.2547817213485636	No Hit
GTGAGTTTCTTCTTTCTTTGGCTTATCTTCTATCTTCTTATCATCTTTCT	49271	0.24677800217352566	No Hit
GTTTCTTCTTTCTTTGGCTTATCTTCTGTCTTCTTATCATCTTTCTTCTC	47996	0.2403920560232294	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	47575	0.23828344164732768	TruSeq Adapter, Index 2 (100% over 50bp)
ATACCATCGGAGCCACTATACATTGATGATGTGAATGAGTAGTATTGTGG	33988	0.17023179431864158	No Hit
GGAAGATATCAGCGCAGCGGCATGAGTACATCCACTCGTTATCGTACCAG	30644	0.15348308535660976	No Hit
CTGTATACCATCGGAGCCACTATACATTGATGATGTGAATGAGTAGTATT	29269	0.14659628068472166	No Hit
GCTGTATACCATCGGAGCCACTATACATTGATGATGTGAATGAGTAGTAT	27531	0.13789135957945514	No Hit
GTGGATTTTCTTCATCATCGACTTCGTCTTCGCTTTGGGCAGCTTCCTTT	27422	0.13734542379092002	No Hit
CCTTAGTGAGTTTCTTCTTTCTTTGGCTTATCTTCTGTCTTCTTATCATC	24210	0.12125784807738946	No Hit
GTTCGTAGTCAGCTGATGGTTCGAAGAGCTGAGCCTTGAGCCAGTCCTTG	23493	0.11766669247757582	No Hit
CACAAAGTTTTTTTCTTTCCTTAGTGAGTTTCTTCTTTCTTTGGCTTATC	23165	0.11602387652675451	No Hit
GTTTACAAACATATTAATATTAAAAGAAGATTAGAAATTCAGCACAAAGT	23111	0.11575341292509492	No Hit
GTAAACGTTTGTCTTGATGTCACCAAGATCGTGGATAGCGACAACTTGGA	22633	0.11335930919188582	No Hit
GTTTAGTTTAAAGGTACTTCTCAAGGCGGTGGAAGATATCAGCGCAGCGG	22345	0.11191683664970124	No Hit
GTACTGTCACACCCATGCTTCTCGACCGAGATCGAGTTTAGCGCAATTTG	21859	0.10948266423476481	No Hit
GGGAGCTTGATGGAGCCGACGAAACGCTTATCCTTAGAAACGTTGTAGCC	21596	0.10816540632297822	No Hit
CTAGCATACATTAGTCATTGTTTTTCTTCCTTCTTTTCTTCTTTCTTCAC	20868	0.10451915628578946	No Hit
CTTTGGAACTGTAAGCGTAAGAACACCATCAGCGAAAGCAGCATTGATTG	20116	0.10075270020341869	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CTTCT	16256580	6.2795296	12.591199	9
TCTTC	14466075	5.5879	12.8602085	8
TTCTT	20133060	5.5777984	10.843492	7
GAAGA	5908175	3.8396363	11.085944	95-97
CCTTC	6642545	3.5774808	6.26725	55-59
CATCA	5865140	3.5175128	7.2224016	80-84
GCAGC	4269630	3.4742203	5.3320556	40-44
CTTGA	7684940	3.449395	6.8966603	1
CACCA	4070970	3.4040828	4.7259936	9
TCATC	6644540	3.198104	5.9168115	80-84
TCCTT	8241495	3.1834934	5.0292106	55-59
CAGCA	3874655	3.0214128	6.748053	1
CCTTG	5981335	3.0041094	3.8755448	85-89
TGGAA	5406770	2.819973	7.1422415	2
CTTTG	7771690	2.7995503	5.9653487	1
GGAAG	4126855	2.7986329	8.001951	1
CAGCG	3366015	2.7389438	5.154315	40-44
TCTGG	5563955	2.6060102	5.2033024	85-89
CTGGA	4437735	2.5899036	5.232021	1
GCCTT	5154965	2.5890677	7.874641	1
TTTCT	9204915	2.5501916	8.488426	6
GAGAA	3902145	2.5359468	5.750287	2
CTTCC	4595555	2.4750316	5.6615343	50-54
ATCAT	5603995	2.410517	5.4735727	60-64
TCAGC	3721580	2.329028	5.7425833	9
GAGCA	3123995	2.2717588	7.096061	9
CAAGG	3067100	2.230385	5.0883145	9
AGAGC	2965665	2.1566217	7.060442	8
TCAAC	3507365	2.1034796	5.0666556	8
TGGAG	3797040	2.0665326	5.121501	2
GGAGA	3025330	2.0516322	9.95889	1
CTGGG	3303465	2.011787	7.3343477	1
AAGAG	3063735	1.9910762	6.3506904	95-97
AGAGA	3055335	1.9856174	5.5507755	3
AGAAA	3092810	1.9261937	5.579161	3
GGCGG	2399060	1.8996481	6.497377	1
TGGGA	3402880	1.8520116	9.056853	2
GTGGA	3297820	1.7948328	12.359215	1
GAGAG	2645925	1.7943382	6.4960117	2
GCTTG	3812685	1.7857615	5.6014695	1
GCTGG	2882195	1.7552366	5.9679	1
GGATG	3168690	1.724554	5.8862715	1
GTTCT	4760370	1.7148002	6.1456823	1
CAAAG	2445035	1.7039112	9.065539	4
GCCAC	1938475	1.691424	5.0097623	15-19
GTTGA	4003780	1.6758978	8.072531	1
TTTGG	4927150	1.6551732	5.265521	2
GGCGA	2134270	1.6195387	6.0017986	1
GAAAG	2476320	1.6093241	6.709026	4
GCCAG	1956820	1.5922747	6.631831	1
GAGTT	3482180	1.4575671	8.894029	3
ACAAA	2039725	1.362208	9.245393	3
TGAGT	3218590	1.3472339	8.519921	2
GGGAA	1941225	1.3164446	7.319673	1
GGAGT	2373770	1.2919201	5.080787	1
GTGAA	2466120	1.2862378	5.7320952	1
AAGTT	3134125	1.2571979	7.1458187	6
GTTTC	3473460	1.2512242	9.382183	5
GCTGT	2499685	1.170787	7.102331	3
GGAGG	1602595	1.1340718	5.1455126	1
TACCA	1881360	1.128312	8.249456	9
GTGAG	2071495	1.1274077	14.119494	1
CTGTA	2498445	1.1214303	6.3687677	4
GTGGG	1949390	1.1070975	9.76992	1
TGCTG	2331520	1.0920227	7.5738926	2
AGTTT	3345505	1.0770088	6.9685345	4
ATACC	1769310	1.0611122	8.255275	8
GTTGG	2389795	1.0438259	6.466296	1
GATTT	3207540	1.0325942	5.3303394	4
AAAGT	2050120	1.024699	8.803334	5
GTAGA	1863295	0.9718264	5.1888704	1
GTGCT	2068330	0.96875143	10.408317	1
GTTCA	2125465	0.9540177	5.6404247	1
GTCGA	1600755	0.93421555	6.495323	1
GTTTG	2770755	0.9307773	6.38887	1
GCCGT	1394370	0.91057444	5.762475	1
GTAAA	1809905	0.90463376	6.0868073	1
GTCAA	1596250	0.89275664	5.6762958	1
GGGAG	1240480	0.8778223	7.4232826	1
TAAAG	1724545	0.8619688	5.406735	9
CACAA	1138360	0.8506809	9.052551	2
TGTAT	2276270	0.7327931	5.402312	5
TTAAA	1876455	0.7213323	5.277428	8
GCACA	895730	0.69848037	9.790016	1
TTTAA	2235070	0.6895387	5.109148	7
GTTTA	1819275	0.58567405	10.698091	1
TATAC	900260	0.38724017	5.424607	7
GTATA	901885	0.36177492	5.3582454	6
>>END_MODULE