FastQCFastQC Report
Thu 31 Jul 2014
Undetermined_Undetermined_L005_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename Undetermined_Undetermined_L005_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6632747
Filtered Sequences 0
Sequence length 101
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 126284 1.9039471880956713 No Hit
GTCGATACCTGTCCAAAACAGAACGACCCGCGAACCAAAGATCACCACTC 7844 0.11826170966569356 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTTTG 1286365 1.7093384 5.0167055 9
TACAC 917135 1.4056798 5.2925253 5
GTCCA 466930 0.9329343 5.2022433 1
GTGTA 547210 0.8021166 5.3797226 1
Produced by FastQC (version 0.10.1)