##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Undetermined_Undetermined_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6632747	
Filtered Sequences	0	
Sequence length	101	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.727794833724246	31.0	31.0	34.0	23.0	34.0
2	30.00485650967842	31.0	31.0	34.0	25.0	34.0
3	30.10023554343321	33.0	31.0	34.0	25.0	34.0
4	33.36733392665211	37.0	35.0	37.0	26.0	37.0
5	33.16058127952114	37.0	35.0	37.0	25.0	37.0
6	33.256322908140476	37.0	35.0	37.0	25.0	37.0
7	33.28990748478722	37.0	35.0	37.0	25.0	37.0
8	33.308559786767084	37.0	35.0	37.0	25.0	37.0
9	34.96320875800027	39.0	35.0	39.0	27.0	39.0
10-14	35.093284758185405	39.2	35.4	39.4	25.4	39.4
15-19	36.09223834408278	40.0	36.8	41.0	25.8	41.0
20-24	35.974074120421	40.0	36.4	41.0	25.4	41.0
25-29	35.665794941371956	40.0	36.0	41.0	24.0	41.0
30-34	35.279440991794196	39.8	35.4	41.0	22.4	41.0
35-39	34.993979025583215	39.0	34.8	41.0	21.4	41.0
40-44	34.91923120239623	39.0	34.6	40.8	21.8	41.0
45-49	34.68763494220419	39.0	34.0	40.4	21.2	41.0
50-54	33.95284110791502	38.0	33.0	39.6	20.4	40.6
55-59	34.4701292541386	38.4	34.0	40.4	21.4	41.0
60-64	33.969421086014584	37.2	33.0	40.0	21.0	41.0
65-69	33.25257450646014	35.8	32.6	39.2	20.0	41.0
70-74	32.452840761150696	35.0	32.0	37.6	20.0	39.8
75-79	31.572084145528237	35.0	31.0	36.4	18.2	38.6
80-84	30.87351750338133	35.0	30.6	35.2	17.2	36.8
85-89	30.33557547121879	34.2	30.0	35.0	15.2	36.0
90-94	29.970105689241574	34.0	29.0	35.0	14.0	35.2
95-99	29.57318634345619	34.0	29.0	35.0	10.8	35.0
100-101	28.75969790495552	33.5	26.5	35.0	9.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	128025.0
3	9234.0
4	10120.0
5	12090.0
6	8134.0
7	12492.0
8	20288.0
9	18068.0
10	15183.0
11	20626.0
12	32906.0
13	43376.0
14	45798.0
15	43788.0
16	42513.0
17	42057.0
18	42607.0
19	43568.0
20	45160.0
21	47391.0
22	49257.0
23	52295.0
24	55183.0
25	59135.0
26	65137.0
27	73243.0
28	83476.0
29	97341.0
30	116018.0
31	141886.0
32	180662.0
33	238456.0
34	331660.0
35	513019.0
36	927825.0
37	1555491.0
38	1249989.0
39	159144.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.22747473934533	24.835636463252975	18.57655040484373	23.360338392557964
2	18.072221740469843	25.479110469736394	35.471695605042605	20.976972184751162
3	18.283134851187132	27.140944141828932	31.10145753701405	23.474463469969887
4	15.75177647652807	20.681078491020067	38.149405341005696	25.41773969144617
5	15.813095003189046	33.86659104236087	35.2148183576247	15.105495596825392
6	24.871681004017827	36.041896653661155	23.151617081896617	15.934805260424403
7	23.73965068428476	30.724755608155007	27.66773531489875	17.867858392661475
8	21.937988833762326	33.06291267206326	26.27281742330983	18.726281070864577
9	22.60129079245674	19.028875176765116	26.84079988560478	31.529034145173362
10-14	21.42676714409346	25.579211961074517	31.443105073995568	21.55091582083645
15-19	20.987670291441262	28.951820404161026	28.99484286228553	21.065666442112178
20-24	21.031050163259497	27.945848121868632	29.887142041046143	21.135959673825727
25-29	21.066783575632098	28.21091507728705	29.459393465900206	21.26290788118064
30-34	20.994486546440505	28.0594015934241	29.601724708630933	21.344387151504463
35-39	21.132089834804948	28.166381055273554	29.498249261257932	21.20327984866357
40-44	20.92038027738935	28.04254392558738	29.7933004466404	21.24377535038287
45-49	21.036384082755077	27.805861569692713	29.85716271677251	21.300591630779696
50-54	20.919172920584742	27.567530361424662	30.158757645481103	21.35453907250949
55-59	20.973632094561427	27.52233769281551	30.207889674688527	21.29614053793454
60-64	20.842920516587146	27.304088150494138	30.256696221338302	21.596295111580414
65-69	20.88506915772313	27.38448985115238	30.078480720420806	21.65196027070368
70-74	20.929184980803146	27.17686183084099	30.043448734927942	21.85050445342792
75-79	21.053672921917048	27.347478019537558	29.813668360618916	21.785180697926478
80-84	20.9739807707518	27.322819404912675	29.748121399655346	21.955078424680174
85-89	21.235596315271817	27.229933792572414	29.711635893389253	21.822833998766516
90-94	21.26653195994067	26.93984464309631	29.96468066957655	21.828942727386476
95-99	21.553636409339425	26.797983603433735	29.825892771248103	21.82248721597874
100-101	21.68451234375108	26.448271834438415	29.957105252257747	21.910110569552756
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	56.587813131903296
2	39.049193925221005
3	41.75759832115702
4	41.16951616797424
5	30.918590600014433
6	40.80648626444223
7	41.607509076946236
8	40.6642699046269
9	54.13032493763011
10-14	42.97768296492991
15-19	42.053336733553444
20-24	42.16700983708523
25-29	42.32969145681274
30-34	42.33887369794497
35-39	42.335369683468514
40-44	42.16415562777222
45-49	42.33697571353478
50-54	42.27371199309423
55-59	42.269772632495965
60-64	42.43921562816756
65-69	42.537029428426806
70-74	42.77968943423107
75-79	42.838853619843526
80-84	42.92905919543198
85-89	43.05843031403833
90-94	43.09547468732715
95-99	43.376123625318165
100-101	43.59462291330384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111667.0
1	60313.5
2	7823.5
3	5966.0
4	5168.0
5	4999.0
6	4931.0
7	4902.0
8	4666.5
9	4361.5
10	4011.5
11	3755.0
12	3614.5
13	3526.0
14	3580.5
15	3732.5
16	3974.0
17	4338.0
18	4836.0
19	5570.0
20	6848.0
21	8665.0
22	11322.0
23	14947.0
24	19542.0
25	25070.0
26	31888.0
27	40077.5
28	49643.5
29	60530.0
30	72336.5
31	85257.0
32	98702.0
33	114257.5
34	137457.0
35	163085.5
36	192412.0
37	231633.0
38	274541.5
39	299821.0
40	310178.0
41	338377.0
42	363767.5
43	367995.5
44	369781.0
45	376785.5
46	378559.0
47	369701.5
48	325279.5
49	241720.0
50	177067.0
51	145553.5
52	119086.5
53	104899.5
54	99460.5
55	79667.0
56	59243.0
57	47952.0
58	38871.0
59	31937.5
60	26288.5
61	20268.5
62	16414.0
63	13973.0
64	12581.0
65	10437.5
66	7402.5
67	5374.0
68	4463.5
69	4404.5
70	4611.0
71	4190.5
72	4498.5
73	4206.0
74	2500.5
75	1606.0
76	1164.0
77	894.5
78	706.0
79	549.5
80	410.5
81	320.5
82	268.0
83	204.5
84	173.5
85	142.0
86	117.0
87	94.5
88	86.0
89	78.0
90	61.5
91	60.5
92	48.5
93	43.0
94	41.5
95	39.5
96	40.0
97	42.0
98	40.0
99	27.5
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.5873442783208827
2	2.4621774356838877
3	2.5660710411538386
4	2.710867759617546
5	2.657556514668809
6	2.665049639312339
7	2.761540580396026
8	3.0012753388603546
9	2.7880605124844955
10-14	2.7235231496090537
15-19	2.332894651341292
20-24	2.3824849643744894
25-29	2.615450280253415
30-34	2.891335972863129
35-39	2.7161687306933313
40-44	2.6637651036591627
45-49	2.8270488833661225
50-54	3.009811771804352
55-59	3.043167484000219
60-64	2.7261284050183128
65-69	2.6700520915391466
70-74	2.7102315224747757
75-79	2.981220299824492
80-84	2.8990582634917326
85-89	2.867240375669387
90-94	2.8080190605792743
95-99	2.8341650902710445
100-101	2.996382946613221
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6632747.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	58.89859402808549
#Duplication Level	Relative count
1	100.0
2	9.87808152222551
3	3.808317442396789
4	2.2869180303040735
5	1.6948802500326998
6	1.3265776774220197
7	1.100777221690612
8	0.9252311356799141
9	0.7510618817163588
10++	15.911359552804951
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	126284	1.9039471880956713	No Hit
GTCGATACCTGTCCAAAACAGAACGACCCGCGAACCAAAGATCACCACTC	7844	0.11826170966569356	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CTTTG	1286365	1.7093384	5.0167055	9
TACAC	917135	1.4056798	5.2925253	5
GTCCA	466930	0.9329343	5.2022433	1
GTGTA	547210	0.8021166	5.3797226	1
>>END_MODULE