##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Undetermined_Undetermined_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6632747	
Filtered Sequences	0	
Sequence length	101	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94865656718099	33.0	31.0	34.0	30.0	34.0
2	32.220604524791916	34.0	31.0	34.0	30.0	34.0
3	32.38924234559226	34.0	31.0	34.0	30.0	34.0
4	35.97171986207223	37.0	35.0	37.0	35.0	37.0
5	35.56520473342342	37.0	35.0	37.0	33.0	37.0
6	35.635011557051705	37.0	35.0	37.0	33.0	37.0
7	35.66357317714667	37.0	35.0	37.0	33.0	37.0
8	35.7024156054799	37.0	35.0	37.0	33.0	37.0
9	37.51021590300369	39.0	37.0	39.0	35.0	39.0
10-14	37.68906053555186	39.2	37.2	39.4	34.2	39.4
15-19	38.70826793182373	40.0	38.0	41.0	34.0	41.0
20-24	38.60543711376297	40.0	38.0	41.0	34.0	41.0
25-29	38.197916971655935	40.0	38.0	41.0	33.2	41.0
30-34	38.017896566837244	40.0	38.0	41.0	33.0	41.0
35-39	37.70986784208715	40.0	37.4	41.0	32.0	41.0
40-44	37.31275445904992	40.0	36.8	41.0	31.0	41.0
45-49	37.045882648622054	39.2	36.0	41.0	30.8	41.0
50-54	36.81611510283748	39.0	35.4	41.0	30.4	41.0
55-59	36.248517725762795	38.4	35.0	40.2	29.6	41.0
60-64	35.37251009272628	37.4	34.4	40.0	28.0	41.0
65-69	33.80791925276209	35.6	33.4	39.0	25.4	40.6
70-74	33.210366926403196	35.0	33.0	37.4	25.0	39.6
75-79	32.19456979137	34.8	32.4	36.0	23.8	38.6
80-84	32.23760610799719	35.0	33.0	35.4	25.0	37.0
85-89	31.787183862131332	35.0	33.0	35.0	23.6	36.0
90-94	31.51770736920917	35.0	32.2	35.0	23.0	35.4
95-99	31.251125363292164	35.0	32.0	35.0	20.4	35.0
100-101	30.822245292938206	34.5	31.5	35.0	19.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	7.0
10	30.0
11	115.0
12	433.0
13	988.0
14	1490.0
15	3179.0
16	6714.0
17	12128.0
18	18729.0
19	27064.0
20	35944.0
21	45401.0
22	57075.0
23	70687.0
24	80710.0
25	86499.0
26	78426.0
27	86396.0
28	95254.0
29	107296.0
30	122825.0
31	144401.0
32	176167.0
33	224256.0
34	314599.0
35	511594.0
36	991989.0
37	1707224.0
38	1421364.0
39	203586.0
40	173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.11940134384408	25.999772199009612	16.869693289594736	24.011133167551566
2	18.696798842670237	25.916985844035047	34.22368729261526	21.16252802067946
3	18.16784207207059	27.74832207530304	30.578597374511645	23.505238478114723
4	15.682214015685577	22.136160818629676	36.697569353863415	25.48405581182133
5	15.45542141136998	35.77276503988468	33.57149006286536	15.200323485879982
6	24.880701766553134	38.19024003176964	21.25259715167788	15.676461049999343
7	23.888277360797872	32.244558702450135	25.748909162372698	18.118254774379302
8	21.972359265324005	35.167178847617734	24.326346233317807	18.53411565374045
9	22.68450764065025	20.196292727583305	25.111134195228612	32.008065436537834
10-14	21.367469617038008	27.299548739006628	29.575848438060433	21.75713320589493
15-19	20.950750008081204	30.875318604475012	27.006891578227744	21.167039809216046
20-24	21.027947683262727	29.76808624991014	27.954947264971985	21.249018801855147
25-29	21.129768459445067	30.074205027802154	27.551760922789718	21.244265589963067
30-34	21.072899717711454	29.936547725478547	27.595598251572707	21.39495430523729
35-39	21.269926939458287	29.98084554182128	27.53141081919735	21.21781669952308
40-44	21.0647620398543	29.9240381750237	27.797483281269926	21.213716503852076
45-49	21.083931526674107	29.795188350438057	27.75137737345489	21.369502749432947
50-54	20.927355451618993	29.577145514149585	28.046360155966504	21.44913887826492
55-59	20.99393727438705	29.664464790266464	27.945956129860217	21.395641805486264
60-64	20.82338110685223	29.48011603706458	27.964581218845503	21.73192163723769
65-69	21.035492760358952	29.518176179664046	27.552257431355017	21.89407362862199
70-74	21.016745662544267	29.47036560824713	27.342691429409783	22.170197299798826
75-79	21.07259477666961	29.618148986677717	27.14048137540393	22.16877486124874
80-84	20.99113877601869	29.69532860374838	26.991446859307665	22.322085760925265
85-89	21.056832516945747	29.549968366298668	26.956143702934167	22.437055413821422
90-94	21.06887021207243	29.222611022720596	27.21096052388206	22.497558241324914
95-99	21.16126761843747	29.120266838494484	27.116894994898477	22.60157054816957
100-101	21.14333546018212	28.79215167284659	27.4375863428321	22.626926524139193
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	57.13053451139566
2	39.8593268633497
3	41.67308055018532
4	41.166269827506916
5	30.655744897249964
6	40.55716281655248
7	42.006532135177174
8	40.50647491906446
9	54.69257307718808
10-14	43.12460282293294
15-19	42.11778981729725
20-24	42.276966485117875
25-29	42.37403404940813
30-34	42.46785402294875
35-39	42.48774363898137
40-44	42.27847854370638
45-49	42.45343427610705
50-54	42.37649432988391
55-59	42.389579079873315
60-64	42.55530274408992
65-69	42.929566388980945
70-74	43.18694296234309
75-79	43.24136963791835
80-84	43.31322453694396
85-89	43.493887930767166
90-94	43.56642845339734
95-99	43.76283816660704
100-101	43.770261984321316
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	132.0
1	70.0
2	5.0
3	3.5
4	6.5
5	13.5
6	38.0
7	68.5
8	104.0
9	187.5
10	371.5
11	582.0
12	889.5
13	1341.0
14	1949.5
15	2833.0
16	3849.0
17	5026.0
18	6586.0
19	8553.5
20	11020.0
21	14230.5
22	18194.5
23	23265.5
24	28816.5
25	35010.5
26	42214.5
27	49704.5
28	58749.0
29	68972.0
30	79919.0
31	92046.5
32	104446.0
33	118168.0
34	140555.5
35	163744.5
36	190342.5
37	224471.5
38	257641.0
39	280593.5
40	298717.5
41	331996.0
42	343586.0
43	339706.5
44	365604.0
45	386104.5
46	374172.5
47	370118.5
48	348627.0
49	285725.0
50	226268.0
51	175540.5
52	129449.0
53	106715.5
54	93684.5
55	71414.0
56	56773.5
57	49798.5
58	45607.5
59	42471.5
60	34022.0
61	25165.5
62	20450.0
63	14654.0
64	9633.5
65	7338.0
66	6031.0
67	5199.5
68	4740.0
69	4537.0
70	4330.5
71	4193.5
72	3740.5
73	2818.5
74	2263.0
75	1830.5
76	1292.5
77	950.0
78	669.0
79	467.5
80	392.5
81	324.5
82	233.5
83	174.5
84	152.5
85	132.0
86	95.0
87	52.5
88	33.0
89	26.5
90	19.0
91	12.5
92	8.0
93	10.0
94	6.0
95	3.0
96	1.5
97	1.5
98	1.5
99	2.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4597341041351344
2	0.17033666443179574
3	0.0
4	0.017745287133671767
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010825077452826107
20-24	6.00053039864177E-4
25-29	0.18309759139011333
30-34	0.00686291818457722
35-39	0.061926076782364836
40-44	0.13130607876344447
45-49	0.1310196212821023
50-54	0.23570626167408468
55-59	0.22611144372007555
60-64	0.6169721233148197
65-69	2.5590181564290027
70-74	2.476590770008263
75-79	2.584696808124899
80-84	2.360653888954305
85-89	2.272602889873532
90-94	2.1368039516658786
95-99	2.148868334643248
100-101	2.4941777516917196
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6632747.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	59.639306305897314
#Duplication Level	Relative count
1	100.0
2	10.85669694328056
3	3.884873878687326
4	2.3993982171479993
5	1.7863670875397204
6	1.3308118444363206
7	1.1395911251089677
8	0.996175585613453
9	0.7662889120103484
10++	17.443266499817216
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CTTTG	1014525	1.6517464	5.7362385	9
TACAC	963355	1.3318293	5.2557707	5
GTGTA	504845	0.7908492	6.278918	1
>>END_MODULE