FastQCFastQC Report
Thu 31 Jul 2014
Undetermined_Undetermined_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename Undetermined_Undetermined_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8489704
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 284849 3.355228874881857 No Hit
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 69476 0.8183559756618134 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 67017 0.7893914793731324 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 55382 0.6523431205610938 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 41997 0.49468155780225087 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 41410 0.4877673002498085 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 31421 0.3701071321214497 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 29930 0.35254468235877245 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 29478 0.34722058625365504 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 28960 0.34111907788540097 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 25077 0.29538132307086323 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 23902 0.28154102899229466 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 23509 0.2769118923345266 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 23117 0.27229453465044245 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 20395 0.2402321682828989 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 20351 0.23971389344080782 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 19172 0.22582648346750367 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 17995 0.21196263144156735 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 17243 0.20310484323128344 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 16392 0.19308093662629464 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15904 0.1873327974685572 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 15798 0.18608422625806506 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 15688 0.18478853915283736 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 14769 0.17396366233734414 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 14238 0.16770902731119955 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 14185 0.16708474170595347 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 13188 0.15534110494311698 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 12702 0.14961652373274734 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12617 0.14861531096961683 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 12273 0.14456334402235932 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 11647 0.13718970649624534 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 11179 0.13167714681218567 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 10995 0.12950981565435024 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 10559 0.12437418312817503 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 10370 0.12214795710192017 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 9796 0.11538682620736837 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 9035 0.10642302723392948 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 8883 0.10463262323397848 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 8594 0.10122849983933481 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 8494 0.10005060247094599 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AGCAG 3036360 4.467032 45.808006 20-24
TTTTT 5097095 4.3139467 7.5482793 7
TAGCA 3254215 3.7420948 29.04581 20-24
GCAGA 2467100 3.6295485 29.867077 25-29
CACGG 1816225 3.5032148 35.019955 45-49
ACTTG 2750900 3.3934965 74.006424 8
CCTGC 1670555 3.3021135 36.887547 15-19
GTTTC 2391190 3.164395 27.465878 40-44
TCACG 2090885 3.1523185 27.695179 45-49
CTGCG 1500655 3.1051462 37.948166 15-19
AGAGA 2713470 3.0448055 18.98556 90-94
GAAGT 2509940 3.0213556 23.940838 7
GAACC 2045320 2.8744664 25.51074 70-74
GCAGC 1452880 2.8023794 16.280916 20-24
GCGTT 1633520 2.765657 28.930746 15-19
CGGAT 1745985 2.755563 26.336847 45-49
AACCA 2645845 2.709312 17.746265 70-74
CTTGA 2185500 2.696022 23.07622 55-59
AACTT 2977330 2.6760752 54.21375 7
ACGGA 1807515 2.6591802 26.38216 45-49
AGTAG 2200350 2.6486847 23.78225 9
ACCAT 2360150 2.5926158 25.21309 75-79
AGAGC 1759395 2.588387 42.97673 95-97
CCATG 1713125 2.5827894 28.038008 75-79
TTCTC 2019610 2.5531375 22.214357 30-34
TGCGT 1501705 2.5424855 30.858938 15-19
CATGC 1681055 2.5344388 27.67306 75-79
AGAAG 2246565 2.5208879 15.7456 6
ACCTG 1630075 2.457579 21.37349 15-19
ATAGC 2062220 2.3713934 20.773508 80-84
GAGCC 1220885 2.354897 49.990074 95-97
AGCAC 1671535 2.3491533 23.587612 80-84
AGGAG 1518105 2.3379629 16.752687 90-94
GCTGC 1118680 2.3147657 25.377417 1
CAGCA 1629380 2.2899091 14.108042 20-24
TGCAT 1834605 2.2631595 21.166866 75-79
AAGGA 2001900 2.2463474 15.8935 70-74
TGAAG 1858800 2.237542 16.882881 60-64
AAGTT 2373535 2.2332478 17.98778 60-64
CTTGT 1683245 2.2275321 79.39748 9
TTGAA 2340290 2.2019677 16.326645 55-59
GAGAG 1429725 2.201853 33.6495 95-97
GACTC 1455965 2.1950827 27.330717 25-29
CTCAG 1447925 2.182961 27.458153 35-39
TTTCA 2259690 2.1788342 16.98146 40-44
TTCAC 1845860 2.1752112 17.345684 45-49
GGAAC 1478505 2.175147 26.896328 70-74
AAACT 2564430 2.1486127 28.637177 6
CAGAC 1524730 2.1428354 25.630955 25-29
ACCAG 1524590 2.1426387 11.352085 55-59
CACTG 1420015 2.1408825 26.79467 85-89
ACAGG 1455140 2.1407728 11.14985 90-94
GCACT 1419370 2.1399102 20.24153 85-89
CAGAA 1981285 2.1237895 12.00158 6
CAGTA 1828775 2.1029494 21.076208 35-39
CTCAT 1777790 2.0949957 16.648249 30-34
TCTCA 1776170 2.0930867 16.655647 35-39
AGGAA 1844540 2.0697725 20.727856 70-74
GGAGG 973035 2.0566742 13.846823 90-94
GGATT 1589455 2.0525358 17.932049 50-54
TGCTG 1206865 2.043302 6.055746 1
CTGAA 1772570 2.0383184 20.62957 85-89
ATGCA 1763350 2.0277157 21.219646 75-79
GATGA 1681725 2.024387 8.695975 15-19
CATTC 1710035 2.0151515 21.919687 30-34
TAACC 1820375 1.9996754 20.04739 10-14
TCAGT 1609390 1.9853355 22.817717 35-39
GCATA 1710395 1.9668219 15.972067 80-84
GAAGA 1738620 1.9509188 7.9686193 50-54
TGATG 1506215 1.9450444 10.399756 30-34
TTTCC 1537710 1.9439323 9.354366 40-44
GTTAC 1571465 1.9385515 20.756912 60-64
AGTTT 1899115 1.9168869 18.883448 40-44
AAGAG 1707825 1.9163635 8.887196 50-54
TAGAG 1587710 1.9112157 21.243332 90-94
ACTGA 1647975 1.8950434 20.933245 85-89
TTAGC 1535135 1.893735 22.739134 20-24
TCATT 1949285 1.8795362 17.987146 30-34
AACCT 1689475 1.8558819 18.837137 10-14
GGCTC 895670 1.8533148 15.4125395 65-69
CTGCA 1223585 1.8447354 14.979077 3
CGTTA 1495080 1.8443232 17.081608 20-24
TGTTG 1319595 1.8280464 9.592877 35-39
CCAGA 1299015 1.8256187 14.38071 55-59
TGAAT 1932870 1.8186281 16.261034 85-89
CATAG 1577170 1.8136234 20.868938 80-84
CCAAA 1758895 1.8010863 23.370775 4
TTGTA 1776705 1.7933317 23.350033 9
CAGGC 928335 1.7906137 12.593729 65-69
GTAGT 1372545 1.7724302 17.712626 40-44
CCATC 1223955 1.7627691 10.8284445 75-79
TGACC 1167905 1.7607893 34.05748 1
GTAAC 1527765 1.7568115 21.100504 10-14
GCTGG 809495 1.7534173 5.5729904 1
GTTAG 1353890 1.7483401 23.672861 20-24
TAAGG 1446160 1.740824 20.241581 65-69
GTAGG 1053370 1.7402864 17.278618 7
AGGCT 1099160 1.7347255 11.702254 65-69
ACCAA 1693965 1.7345988 12.484552 3
TGCAG 1095670 1.7292175 16.815634 4
TGTTT 1592620 1.7244933 10.806633 35-39
CTAAG 1497920 1.7224921 20.736616 65-69
AAGAA 2103745 1.7199918 8.741576 45-49
ACTCA 1553695 1.7067286 18.680767 25-29
TTACT 1766975 1.7037495 13.505183 65-69
AGACT 1480200 1.7021155 21.107958 25-29
GTTGT 1223965 1.6955693 10.276049 35-39
GAATA 1921255 1.6850868 12.158147 90-94
ATTCT 1728110 1.6662751 17.904835 30-34
AATAG 1894665 1.6617653 16.06054 90-94
TTAAA 2421930 1.6603575 12.953852 50-54
ATGAT 1751625 1.6480955 7.4873347 15-19
TGTAA 1744390 1.6412883 17.469378 10-14
GGAAG 1054905 1.6246101 8.21416 6
GGATG 983080 1.6241592 13.431798 10-14
TTGTT 1483085 1.6058884 8.736415 35-39
ATTAA 2340380 1.6044508 13.012306 50-54
GACCA 1136675 1.5974681 15.113676 2
AGGGA 1032040 1.5893967 16.160229 9
CATCA 1439750 1.5815604 7.482707 80-84
GATTA 1672265 1.573426 17.280094 50-54
GGAGC 776015 1.5668851 15.01217 95-97
TACCA 1423135 1.5633088 8.527068 75-79
AAAGA 1900345 1.5536946 6.9840093 45-49
AGTTA 1645910 1.5486288 16.963766 60-64
TAAAC 1847845 1.5482206 14.477933 50-54
TTCCG 956705 1.5473263 11.717057 40-44
TAGTT 1531560 1.5458924 18.417564 40-44
AGAAA 1883755 1.5401312 6.08095 60-64
AGCTG 972470 1.5347798 6.434308 95-97
CAGGA 1037490 1.5263346 10.227719 90-94
GAGCG 754720 1.5238878 5.864353 90-94
AACAG 1419070 1.5211371 12.1337185 60-64
GATGT 1171975 1.513425 12.190861 30-34
ATACC 1362930 1.4971738 8.107695 75-79
TAAAG 1704995 1.4954103 7.440276 45-49
GCTCA 986870 1.4878525 14.1681795 65-69
GAGAT 1233275 1.4845624 11.470888 25-29
CAGAG 998850 1.4694881 10.885571 25-29
CTACA 1325850 1.4564416 8.805207 85-89
TCCGT 891865 1.4424576 12.046314 40-44
ACTAA 1711145 1.4336863 15.534158 65-69
AGCCA 1017110 1.4294331 11.32894 95-97
TAGGG 864290 1.4279048 17.117308 8
GAGGA 925780 1.4257506 14.305566 95-97
CAAAC 1390105 1.42345 27.447302 5
GAGAA 1267980 1.4228101 10.89176 50-54
TACAG 1230100 1.4145198 8.303268 90-94
TACTA 1573690 1.4144596 16.36698 65-69
GAAAC 1312230 1.4066128 8.027362 60-64
GAGCT 889690 1.4041339 7.640071 95-97
CTCAC 965275 1.3902119 10.567175 65-69
ATGTT 1377075 1.3899617 7.730472 30-34
ATAGA 1558230 1.3666861 15.888821 90-94
TCCTC 876535 1.3542638 6.2897696 1
GCCAA 956190 1.3438169 10.995845 3
GTAAA 1526520 1.3388741 6.5931244 45-49
AGATG 1096310 1.31969 9.055824 25-29
TCAAC 1184655 1.3013393 6.3861747 7
TGATA 1377885 1.2964454 7.5420127 15-19
CAACA 1257405 1.2875669 5.73475 8
AAACA 1645320 1.2850313 6.3802004 60-64
AGAAC 1154850 1.2379131 8.869592 50-54
GGGAT 741725 1.2254137 15.230187 10-14
GATAC 1055450 1.2136858 7.299097 75-79
CAATA 1435545 1.2027743 7.6544037 80-84
TCTAC 1009550 1.189681 10.262463 85-89
AAGTA 1318155 1.1561222 18.065203 8
ATCAA 1377545 1.1541789 6.2100844 80-84
CCGTA 764700 1.1528983 13.912875 40-44
CGGAA 783100 1.1520811 6.7787113 5
GGCCA 589615 1.1372753 12.325613 2
GGATA 928430 1.1176032 8.704703 70-74
CCCAA 821305 1.1026316 7.05331 4
TCAAT 1217775 1.094557 6.474383 80-84
GTCAA 938545 1.0792542 6.483101 6
GCCGA 557070 1.0745013 9.431899 3
CCTCT 695100 1.073943 5.006448 2
CCAAC 796395 1.069189 5.271305 5
CCGAA 749890 1.0538855 22.634048 4
CGTAA 911710 1.0483959 8.42609 40-44
GATAG 855880 1.0302708 11.223416 15-19
TGGCC 494380 1.022968 21.702469 1
TGGCT 572025 0.96847606 5.465362 1
GGTGC 445315 0.9645803 7.966537 2
GGCTG 439100 0.95111823 6.665527 1
TATCT 966745 0.93215317 6.601655 85-89
CCTAA 839090 0.9217372 8.451322 4
GCCCA 494265 0.9107246 5.2993255 2
GGCCT 439695 0.9098141 5.68138 2
ATATC 998170 0.8971723 5.636031 85-89
ATCTA 985435 0.88572586 6.374169 85-89
ACCGA 624915 0.87824726 13.519044 3
ACCTA 786190 0.8636268 5.7090473 3
TGTCA 695925 0.8584896 5.718069 5
GACTG 541980 0.85536826 6.784844 2
GACCT 563125 0.8489942 7.672074 2
CTGTC 492905 0.7971998 6.7400017 4
CGAAC 561060 0.7885063 21.349794 5
GTGCA 498615 0.7869283 6.307156 3
GAACT 684010 0.7865586 19.486063 6
TGACT 630705 0.7780345 9.503077 1
GACCG 402415 0.7761959 18.101988 2
GCTGT 449235 0.7605845 6.2396765 1
TGCGG 344015 0.7451582 9.527549 3
GACTT 591890 0.73015255 6.6112742 6
TGTCC 450100 0.727969 6.6807055 1
GGGTG 305685 0.6931308 8.87675 1
GGCCG 257995 0.6829827 12.046364 2
GTCTA 550850 0.6795258 6.3510656 8
TGCCC 343275 0.6785368 6.091304 1
AGTCT 532385 0.65674746 6.2065187 7
GACCC 346025 0.63758 5.7519464 2
CTAAC 564880 0.6205187 7.663322 5
GGGCC 233095 0.61706567 6.8572946 1
GCGGA 303315 0.6124364 8.174687 4
TAACT 659450 0.592725 6.811392 6
GGCCC 218615 0.5528514 5.4259443 1
Produced by FastQC (version 0.10.1)