FastQCFastQC Report
Thu 31 Jul 2014
Undetermined_Undetermined_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename Undetermined_Undetermined_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8489704
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 127978 1.507449494116638 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 55608 0.6550051686136524 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 50947 0.6001033722730498 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 49476 0.5827765019840503 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 46918 0.5526458873006644 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 41299 0.4864598341708969 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 33395 0.3933588261734449 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 32976 0.3884234361998958 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 32327 0.3807788822790524 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 29831 0.3513785639640675 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 29026 0.34189649014853757 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 24421 0.2876543163342326 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 24147 0.2844268775448473 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 23353 0.27507437243984006 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 23287 0.27429696017670346 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 21308 0.25098637125628875 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 20991 0.24725243659849627 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 20151 0.2373580987040302 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 19651 0.23146861186208612 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 18398 0.21670955783617427 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 17779 0.20941837312584752 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 16893 0.19898220244192258 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 16065 0.1892292122316632 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 15822 0.18636692162647836 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 15533 0.1829627982318347 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 15171 0.17869880975826719 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 15073 0.17754447033724616 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 14347 0.16899293544274335 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 13944 0.16424600904813644 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 13716 0.16156040304820993 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 13614 0.16035894773245332 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 13437 0.15827406939040511 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 12645 0.1489451222327657 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 12582 0.14820304689068076 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 12435 0.1464715377591492 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 12123 0.1427964979697761 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 12011 0.14147725291718063 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 11566 0.1362356096278504 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 11123 0.13101752428588795 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 11028 0.12989852178591857 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 10242 0.12064024847038247 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 9877 0.11634092307576331 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 9590 0.11296035762848741 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 9512 0.11204159768114412 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 9404 0.1107694685232842 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 9284 0.10935599168121764 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 9088 0.10704731283917555 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 9017 0.10621100570761949 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 8949 0.10541003549711508 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 2324100 3.2683954 9.36868 5
ACTTC 2369730 2.9659436 5.7616777 60-64
CTTCT 2325345 2.6460304 5.923229 40-44
CTGGT 2100575 2.644598 10.260456 1
AGCTG 1814110 2.5121276 6.4879065 3
GCTGC 1401715 2.4361942 7.4672194 3
TCTCT 2135460 2.4299586 7.6812963 20-24
GGAAG 1772610 2.417072 5.192245 15-19
CTACT 1920495 2.403683 10.671601 2
TCAAC 1739390 2.394515 6.115436 55-59
CAAGC 1368690 2.3286173 6.3734655 75-79
CTTCA 1850570 2.3161652 5.706876 60-64
CAACT 1681445 2.314746 6.135925 9
TTCAA 2275070 2.2687397 6.0990825 55-59
TGGTG 1999640 2.2537978 7.2376785 6
CCAGC 1031280 2.2021337 5.5043883 1
GCTGG 1408790 2.1919978 8.112978 4
GCAGC 1144005 2.1869392 6.148239 1
ACTGC 1402700 2.1697075 8.716858 4
ATGGT 2123640 2.1302385 6.1844444 1
GGTGG 1529135 2.130009 7.675059 7
GAAGC 1395130 2.1249533 5.7701616 15-19
AGGAA 1748935 2.1224346 5.1045537 40-44
TCCTT 1857090 2.113199 5.660529 40-44
CATGG 1516890 2.100546 10.859283 2
CTGAA 1698970 2.0938618 5.017644 80-84
GGTTC 1662310 2.0928278 5.10286 3
GGTGT 1843910 2.0782743 9.063792 1
TGCTG 1641055 2.0660682 7.384344 6
CAACA 1363510 2.0645983 5.557697 85-89
GCGGC 959665 2.0613143 6.8975563 7
TATGC 1834285 2.055287 6.0671296 30-34
GCTAC 1324385 2.0485692 13.318726 1
CGGTG 1315790 2.0472953 10.3009815 9
CCAAG 1173945 1.9972881 5.956003 75-79
GCTTC 1351860 1.9011288 7.1433396 15-19
CTTGG 1506405 1.8965454 6.0646505 2
CGTGA 1350550 1.870203 9.106981 9
GGTAT 1863735 1.869526 5.0141087 6
CTGTT 1820480 1.8545349 6.0780396 8
ACAAC 1222270 1.8507355 5.4054904 85-89
CAAGG 1212410 1.8466485 5.3171616 10-14
TTGGT 2019765 1.8420084 15.0154295 7
ACCCA 957800 1.8202296 27.937819 1
CTCTG 1283805 1.8054224 6.573842 45-49
TGGCT 1424400 1.7933019 5.6667747 9
GTGGC 1152495 1.7932173 6.6219 8
CTCCT 1120105 1.7595308 6.620964 70-74
TTTCA 1917380 1.7383671 5.332835 8
CCTGT 1213215 1.7061512 6.7003145 1
CATCC 981580 1.6959788 5.7808504 90-94
CTTTC 1480060 1.6841732 6.503199 7
GCTTG 1336065 1.6820894 6.1472025 1
GCTCC 861935 1.6733433 5.1966953 70-74
TACTG 1491295 1.6709721 6.387582 3
TGGTA 1658180 1.6633323 7.7444305 2
CCGCC 608325 1.630335 5.839612 45-49
TTCGG 1289515 1.6234834 8.3872595 7
TCGGT 1280095 1.6116238 9.960696 8
GACGG 941305 1.6109456 8.4884205 3
CCTTA 1251330 1.5661589 6.2503505 95-97
GTAGC 1120635 1.5518233 5.590562 1
CCAAT 1124545 1.5480944 20.746174 3
TGTAG 1542995 1.5477895 5.1212907 3
TATTC 1687270 1.5297409 5.1710896 5
GGTCA 1103730 1.5284138 19.177702 9
TGACG 1103085 1.5275205 6.9196053 2
ATGCC 983700 1.5215949 7.1563225 9
ACGGT 1095955 1.5176471 6.8710246 4
TGGTC 1200265 1.5111188 17.582256 8
GCTGT 1185925 1.4930649 7.217391 7
AGCCT 964545 1.4919659 5.471986 2
CTGTA 1327540 1.4874874 5.8808947 8
CCCAA 769105 1.4616284 28.320747 2
GCAAC 844965 1.4375792 5.070563 1
GGCTG 897775 1.3968874 6.0771265 9
TACTT 1536420 1.3929747 5.728981 5
GGTCG 893125 1.3896521 9.907616 6
CTGAC 878705 1.359188 7.697427 1
GGTAC 979925 1.3569721 8.375093 3
ACTTT 1447950 1.3127646 5.7588224 4
GTGTA 1306760 1.3108203 7.9670568 2
GTATT 1599830 1.2985206 5.189238 4
AAGCC 741930 1.2622806 5.439167 1
ATGGG 1003055 1.2434964 9.640226 3
GCCTG 713540 1.2401395 6.181369 6
GTATG 1226200 1.23001 5.062915 7
GGGTC 784215 1.2201943 9.7280035 5
CGGTA 853930 1.182498 6.5414114 5
TGGCC 651925 1.133052 8.255664 1
ATTGG 1127850 1.1313543 14.339422 6
TGGGT 997085 1.1238163 7.5941324 4
ACATG 895855 1.1040788 9.573344 1
CAATT 1103535 1.1004646 15.008711 4
GTACT 942855 1.0564541 6.583737 4
TCGTG 831630 1.047012 7.893609 8
TGGGG 703520 0.9799683 5.071745 8
GGCCT 541840 0.94172317 6.1042676 5
ATTCG 825410 0.9248587 6.1652374 6
AATTG 997870 0.890852 12.3173275 5
GCCTA 498790 0.77153236 5.013632 3
GTCGT 609200 0.7669753 7.766893 7
Produced by FastQC (version 0.10.1)