##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Undetermined_Undetermined_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	8489704	
Filtered Sequences	0	
Sequence length	101	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.770865509563116	34.0	31.0	34.0	31.0	34.0
2	32.97693794742432	34.0	33.0	34.0	31.0	34.0
3	33.05861606011234	34.0	33.0	34.0	31.0	34.0
4	36.464817265713855	37.0	37.0	37.0	35.0	37.0
5	36.405693414046	37.0	37.0	37.0	35.0	37.0
6	36.388196573166745	37.0	37.0	37.0	35.0	37.0
7	36.39300533917319	37.0	37.0	37.0	35.0	37.0
8	36.367056024568114	37.0	37.0	37.0	35.0	37.0
9	38.03765090043186	39.0	39.0	39.0	37.0	39.0
10-14	38.414417746484446	39.4	38.6	39.4	37.0	39.4
15-19	39.562608119199446	41.0	39.4	41.0	37.0	41.0
20-24	39.54312736934056	41.0	39.4	41.0	37.0	41.0
25-29	39.33058184360728	41.0	39.0	41.0	36.6	41.0
30-34	39.17625283519897	40.0	39.0	41.0	36.0	41.0
35-39	38.91564928529899	40.0	38.6	41.0	35.6	41.0
40-44	38.63009233301891	40.0	38.0	41.0	35.0	41.0
45-49	38.402570077826034	40.0	38.0	41.0	34.4	41.0
50-54	38.17934182393167	40.0	37.4	41.0	34.0	41.0
55-59	37.6382471285218	39.4	36.0	41.0	33.6	41.0
60-64	36.68353282988429	38.2	35.0	40.4	32.4	41.0
65-69	35.005138082552705	36.6	35.0	39.6	30.0	41.0
70-74	34.463296718001004	35.4	35.0	38.8	30.0	40.8
75-79	33.50784373636584	35.0	33.8	36.8	29.4	39.2
80-84	33.38101985652268	35.0	34.0	36.0	30.0	37.8
85-89	32.96965816476052	35.0	34.0	35.4	30.0	36.6
90-94	32.71181718467452	35.0	34.0	35.0	29.4	36.0
95-99	32.491089748240924	35.0	34.0	35.0	29.0	35.0
100-101	32.1287508963799	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	2.0
7	2.0
8	17.0
9	55.0
10	404.0
11	2689.0
12	4539.0
13	6425.0
14	3308.0
15	4219.0
16	5328.0
17	7762.0
18	10905.0
19	15096.0
20	19625.0
21	25578.0
22	34797.0
23	54301.0
24	98892.0
25	105877.0
26	32816.0
27	38293.0
28	46183.0
29	56050.0
30	69332.0
31	88627.0
32	117311.0
33	164901.0
34	259186.0
35	487108.0
36	1255213.0
37	2494199.0
38	2393318.0
39	587172.0
40	171.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	27.743798350296235	27.42465120119662	21.526923560129156	23.304626888377992
2	25.683117679511092	23.762742961187037	23.178149116075822	27.375990243226045
3	25.707939876349045	25.308196846438936	23.642885547010824	25.340977730201192
4	27.147224449757022	23.99970599681685	21.66478360140707	27.188285952019058
5	25.505965814591413	25.246204107940628	23.036716003290575	26.211114074177377
6	25.870807745476164	26.756869261872968	25.324981883938474	22.04734110871239
7	28.84819070252626	22.104975626947653	27.29429671517405	21.752536955352035
8	27.527331930536093	22.66619660709019	31.99338869765071	17.81308276472301
9	29.096621036493147	24.246840643678507	29.237556456620865	17.41898186320748
10-14	28.39309018986246	24.97333593601548	28.941962119855397	17.691611754266663
15-19	24.809441742447234	25.992144916717308	29.92326737314892	19.275145967686534
20-24	22.588118100978527	25.004930175730994	31.414241080280835	20.992710643009644
25-29	22.91014798767868	25.444924791435884	29.741953539253952	21.902973681631487
30-34	23.01303071345366	25.93448767811149	30.545433104117343	20.50704850431751
35-39	23.209905298278237	26.589301417600208	29.18701671512709	21.013776568994462
40-44	23.312503687413695	26.974992946382066	29.149351863088057	20.56315150311618
45-49	22.362365980343775	25.972221494122966	29.81912172527126	21.846290800262004
50-54	22.457146064907636	26.52423572270272	30.25356503025526	20.765053182134384
55-59	22.167958553330465	26.63523112933848	29.893521218172513	21.303289099158537
60-64	21.582070724487675	28.214580795470923	29.414829377161595	20.78851910287981
65-69	22.504483674184492	27.57213501464423	28.934483534102146	20.988897777069134
70-74	22.942313988046298	26.386137269040084	29.153715935645852	21.517832807267766
75-79	22.096934280113427	27.45050186602634	28.8488056664493	21.603758187410936
80-84	23.086697360076943	27.314115723072085	28.257988807537345	21.34119810931363
85-89	22.070636696205334	27.533076802310042	28.769336085775194	21.626950415709434
90-94	24.132472889487982	27.38796010554073	28.54528932522735	19.934277679743932
95-99	22.755790419793982	26.345498138355172	29.155729887079712	21.742981554771138
100-101	23.88924183637363	27.827548370070975	28.379994296140215	19.903215497415182
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	51.04842523867422
2	53.059107922737134
3	51.04891760655023
4	54.33551040177608
5	51.717079888768794
6	47.91814885418856
7	50.600727657878295
8	45.340414695259106
9	46.515602899700625
10-14	46.08470194412912
15-19	44.084587710133775
20-24	43.58082874398817
25-29	44.81312166931017
30-34	43.520079217771176
35-39	44.2236818672727
40-44	43.875655190529876
45-49	44.20865678060578
50-54	43.22219924704202
55-59	43.471247652489005
60-64	42.37058982736748
65-69	43.493381451253626
70-74	44.46014679531407
75-79	43.70069246752436
80-84	44.42789546939057
85-89	43.697587111914764
90-94	44.066750569231914
95-99	44.49877197456512
100-101	43.79245733378881
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	798.0
1	500.0
2	201.5
3	247.0
4	308.5
5	381.0
6	583.5
7	797.5
8	1180.0
9	1566.0
10	1922.0
11	2356.0
12	3524.0
13	5140.0
14	6383.5
15	7145.0
16	8264.0
17	10549.0
18	11560.5
19	13288.5
20	15660.0
21	18496.0
22	20709.5
23	23701.0
24	28436.5
25	30616.5
26	36019.5
27	50777.5
28	59370.0
29	55660.5
30	59761.5
31	69986.5
32	76050.0
33	84707.5
34	101271.5
35	114749.5
36	163605.5
37	211381.0
38	227517.5
39	260131.5
40	299110.0
41	342616.0
42	473534.0
43	614008.5
44	605323.5
45	529552.0
46	498819.0
47	501885.0
48	468382.0
49	389600.5
50	350831.5
51	317800.0
52	253984.0
53	218915.0
54	176442.0
55	129466.5
56	103765.5
57	85758.0
58	71472.5
59	60349.0
60	49187.5
61	38231.0
62	30485.0
63	26343.0
64	20644.0
65	16694.0
66	12736.0
67	6828.5
68	4370.5
69	2785.0
70	1788.0
71	928.0
72	636.5
73	488.5
74	336.5
75	222.5
76	116.5
77	68.5
78	43.0
79	32.5
80	23.5
81	19.0
82	16.0
83	9.5
84	8.0
85	7.5
86	4.5
87	3.5
88	4.0
89	4.5
90	3.0
91	2.5
92	2.0
93	1.0
94	1.0
95	1.5
96	2.5
97	3.5
98	5.0
99	9.0
100	47.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.430521488146112
2	0.2705748044925948
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	4.7115894735552624E-6
15-19	1.97886757889321E-4
20-24	0.0017503554894257795
25-29	0.12270156886506291
30-34	0.036790446404256265
35-39	0.2320410699831231
40-44	0.25571209549826474
45-49	0.23749709059350008
50-54	0.28894293605525
55-59	0.2981658724497344
60-64	0.946982368289872
65-69	3.6373682757372934
70-74	3.6535855667052703
75-79	3.856273434268144
80-84	3.8516819903261648
85-89	3.6356179202478676
90-94	3.4724602883681217
95-99	3.5774769061441956
100-101	3.7462377958053663
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8489704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	78.60129585225243
#Duplication Level	Relative count
1	100.0
2	21.51651265032091
3	10.499814035991188
4	6.980802792321117
5	4.74828102499547
6	3.7259558073222134
7	2.951582601398068
8	2.403227190799073
9	2.056094374350318
10++	35.85004625258681
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT	127978	1.507449494116638	No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG	55608	0.6550051686136524	No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC	50947	0.6001033722730498	No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT	49476	0.5827765019840503	No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA	46918	0.5526458873006644	No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG	41299	0.4864598341708969	No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC	33395	0.3933588261734449	No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT	32976	0.3884234361998958	No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT	32327	0.3807788822790524	No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT	29831	0.3513785639640675	No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT	29026	0.34189649014853757	No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT	24421	0.2876543163342326	No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA	24147	0.2844268775448473	No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG	23353	0.27507437243984006	No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC	23287	0.27429696017670346	No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC	21308	0.25098637125628875	No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC	20991	0.24725243659849627	No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC	20151	0.2373580987040302	No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT	19651	0.23146861186208612	No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT	18398	0.21670955783617427	No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT	17779	0.20941837312584752	No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA	16893	0.19898220244192258	No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA	16065	0.1892292122316632	No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC	15822	0.18636692162647836	No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT	15533	0.1829627982318347	No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA	15171	0.17869880975826719	No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT	15073	0.17754447033724616	No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG	14347	0.16899293544274335	No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA	13944	0.16424600904813644	No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA	13716	0.16156040304820993	No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT	13614	0.16035894773245332	No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT	13437	0.15827406939040511	No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA	12645	0.1489451222327657	No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA	12582	0.14820304689068076	No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG	12435	0.1464715377591492	No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG	12123	0.1427964979697761	No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT	12011	0.14147725291718063	No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA	11566	0.1362356096278504	No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA	11123	0.13101752428588795	No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC	11028	0.12989852178591857	No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA	10242	0.12064024847038247	No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC	9877	0.11634092307576331	No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG	9590	0.11296035762848741	No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT	9512	0.11204159768114412	No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA	9404	0.1107694685232842	No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT	9284	0.10935599168121764	No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC	9088	0.10704731283917555	No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT	9017	0.10621100570761949	No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT	8949	0.10541003549711508	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CTGCT	2324100	3.2683954	9.36868	5
ACTTC	2369730	2.9659436	5.7616777	60-64
CTTCT	2325345	2.6460304	5.923229	40-44
CTGGT	2100575	2.644598	10.260456	1
AGCTG	1814110	2.5121276	6.4879065	3
GCTGC	1401715	2.4361942	7.4672194	3
TCTCT	2135460	2.4299586	7.6812963	20-24
GGAAG	1772610	2.417072	5.192245	15-19
CTACT	1920495	2.403683	10.671601	2
TCAAC	1739390	2.394515	6.115436	55-59
CAAGC	1368690	2.3286173	6.3734655	75-79
CTTCA	1850570	2.3161652	5.706876	60-64
CAACT	1681445	2.314746	6.135925	9
TTCAA	2275070	2.2687397	6.0990825	55-59
TGGTG	1999640	2.2537978	7.2376785	6
CCAGC	1031280	2.2021337	5.5043883	1
GCTGG	1408790	2.1919978	8.112978	4
GCAGC	1144005	2.1869392	6.148239	1
ACTGC	1402700	2.1697075	8.716858	4
ATGGT	2123640	2.1302385	6.1844444	1
GGTGG	1529135	2.130009	7.675059	7
GAAGC	1395130	2.1249533	5.7701616	15-19
AGGAA	1748935	2.1224346	5.1045537	40-44
TCCTT	1857090	2.113199	5.660529	40-44
CATGG	1516890	2.100546	10.859283	2
CTGAA	1698970	2.0938618	5.017644	80-84
GGTTC	1662310	2.0928278	5.10286	3
GGTGT	1843910	2.0782743	9.063792	1
TGCTG	1641055	2.0660682	7.384344	6
CAACA	1363510	2.0645983	5.557697	85-89
GCGGC	959665	2.0613143	6.8975563	7
TATGC	1834285	2.055287	6.0671296	30-34
GCTAC	1324385	2.0485692	13.318726	1
CGGTG	1315790	2.0472953	10.3009815	9
CCAAG	1173945	1.9972881	5.956003	75-79
GCTTC	1351860	1.9011288	7.1433396	15-19
CTTGG	1506405	1.8965454	6.0646505	2
CGTGA	1350550	1.870203	9.106981	9
GGTAT	1863735	1.869526	5.0141087	6
CTGTT	1820480	1.8545349	6.0780396	8
ACAAC	1222270	1.8507355	5.4054904	85-89
CAAGG	1212410	1.8466485	5.3171616	10-14
TTGGT	2019765	1.8420084	15.0154295	7
ACCCA	957800	1.8202296	27.937819	1
CTCTG	1283805	1.8054224	6.573842	45-49
TGGCT	1424400	1.7933019	5.6667747	9
GTGGC	1152495	1.7932173	6.6219	8
CTCCT	1120105	1.7595308	6.620964	70-74
TTTCA	1917380	1.7383671	5.332835	8
CCTGT	1213215	1.7061512	6.7003145	1
CATCC	981580	1.6959788	5.7808504	90-94
CTTTC	1480060	1.6841732	6.503199	7
GCTTG	1336065	1.6820894	6.1472025	1
GCTCC	861935	1.6733433	5.1966953	70-74
TACTG	1491295	1.6709721	6.387582	3
TGGTA	1658180	1.6633323	7.7444305	2
CCGCC	608325	1.630335	5.839612	45-49
TTCGG	1289515	1.6234834	8.3872595	7
TCGGT	1280095	1.6116238	9.960696	8
GACGG	941305	1.6109456	8.4884205	3
CCTTA	1251330	1.5661589	6.2503505	95-97
GTAGC	1120635	1.5518233	5.590562	1
CCAAT	1124545	1.5480944	20.746174	3
TGTAG	1542995	1.5477895	5.1212907	3
TATTC	1687270	1.5297409	5.1710896	5
GGTCA	1103730	1.5284138	19.177702	9
TGACG	1103085	1.5275205	6.9196053	2
ATGCC	983700	1.5215949	7.1563225	9
ACGGT	1095955	1.5176471	6.8710246	4
TGGTC	1200265	1.5111188	17.582256	8
GCTGT	1185925	1.4930649	7.217391	7
AGCCT	964545	1.4919659	5.471986	2
CTGTA	1327540	1.4874874	5.8808947	8
CCCAA	769105	1.4616284	28.320747	2
GCAAC	844965	1.4375792	5.070563	1
GGCTG	897775	1.3968874	6.0771265	9
TACTT	1536420	1.3929747	5.728981	5
GGTCG	893125	1.3896521	9.907616	6
CTGAC	878705	1.359188	7.697427	1
GGTAC	979925	1.3569721	8.375093	3
ACTTT	1447950	1.3127646	5.7588224	4
GTGTA	1306760	1.3108203	7.9670568	2
GTATT	1599830	1.2985206	5.189238	4
AAGCC	741930	1.2622806	5.439167	1
ATGGG	1003055	1.2434964	9.640226	3
GCCTG	713540	1.2401395	6.181369	6
GTATG	1226200	1.23001	5.062915	7
GGGTC	784215	1.2201943	9.7280035	5
CGGTA	853930	1.182498	6.5414114	5
TGGCC	651925	1.133052	8.255664	1
ATTGG	1127850	1.1313543	14.339422	6
TGGGT	997085	1.1238163	7.5941324	4
ACATG	895855	1.1040788	9.573344	1
CAATT	1103535	1.1004646	15.008711	4
GTACT	942855	1.0564541	6.583737	4
TCGTG	831630	1.047012	7.893609	8
TGGGG	703520	0.9799683	5.071745	8
GGCCT	541840	0.94172317	6.1042676	5
ATTCG	825410	0.9248587	6.1652374	6
AATTG	997870	0.890852	12.3173275	5
GCCTA	498790	0.77153236	5.013632	3
GTCGT	609200	0.7669753	7.766893	7
>>END_MODULE