FastQCFastQC Report
Thu 31 Jul 2014
Undetermined_Undetermined_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename Undetermined_Undetermined_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8790778
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 179841 2.04579162390405 No Hit
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 96627 1.0991859878613703 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 95087 1.0816676294180105 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 68575 0.780078850813887 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 60312 0.686082619763575 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 51288 0.5834295895084599 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 41068 0.4671713925661642 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 37479 0.42634451694719167 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 32094 0.3650871401825868 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 31610 0.35958137038610233 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 31475 0.3580456701329507 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25678 0.2921015637068755 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 24451 0.2781437547393416 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 23274 0.26475472364334535 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 23041 0.26210421876197987 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 22846 0.25988598506298305 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21884 0.24894269881459868 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 21748 0.24739562300401624 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 20831 0.23696423684001575 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 20247 0.230320911300456 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 20062 0.2282164331757667 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 19793 0.22515640822689414 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 19247 0.218945353869703 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 18124 0.20617060287496736 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 16983 0.1931910918464782 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 15330 0.1743872954134435 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15230 0.1732497396703682 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 14516 0.16512759166481056 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 14435 0.16420617151291955 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 14124 0.16066837315195537 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 13827 0.15728983259502174 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 13734 0.1562319057539617 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 13252 0.15074888707233877 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 12323 0.1401809942191692 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 11387 0.12953347246398442 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 11106 0.12633694082594282 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 10993 0.12505150283626776 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 10926 0.12428934048840728 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 10505 0.11950023081006027 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 10270 0.11682697481383332 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 10262 0.11673597035438729 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 9978 0.11350531204405342 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 9490 0.10795404001784598 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 9033 0.10275541027199185 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 7142630 5.6517487 9.302454 7
AGCAG 3405055 4.8018484 51.47199 20-24
TAGCA 3673060 4.0006747 31.851381 20-24
GCAGA 2793760 3.939793 33.57364 25-29
CACGG 2039535 3.8636043 40.12179 45-49
ACTTG 3093810 3.6429858 85.47699 8
CCTGC 1847815 3.631768 42.482586 15-19
CTGCG 1683635 3.448006 43.769623 15-19
TCACG 2333425 3.4141004 31.295122 45-49
GTTTC 2606860 3.3184867 30.878826 40-44
AGAGA 3044735 3.1963675 21.134676 90-94
GAAGT 2759550 3.1318717 25.570868 7
GAACC 2288135 3.0967455 28.610418 70-74
CGGAT 1983685 3.0242352 30.173725 45-49
GCGTT 1794905 2.9583044 33.246777 15-19
GCAGC 1560590 2.9563122 18.589003 20-24
ACGGA 2056345 2.8998818 29.552332 45-49
AACCA 2924110 2.8273528 19.750257 70-74
AGTAG 2478655 2.8130782 25.470936 9
AACTT 3337855 2.807978 61.326126 7
CTTGA 2368260 2.788645 25.539593 55-59
TGCGT 1676750 2.7635653 34.986126 15-19
ACCAT 2616105 2.7346387 28.506592 75-79
CCATG 1844425 2.6986306 31.443722 75-79
CATGC 1834850 2.684621 31.262875 75-79
TTCTC 2193800 2.6801488 25.180656 30-34
ACCTG 1799045 2.6322334 24.305866 15-19
AGAGC 1859650 2.6225004 48.08475 95-97
AGAAG 2476435 2.5997653 17.837534 6
GAGCC 1366545 2.588722 58.865906 95-97
AGGAG 1755460 2.5795012 19.652 90-94
ATAGC 2321920 2.5290215 23.004301 80-84
AGCAC 1815895 2.4576194 26.947905 80-84
GCTGC 1184765 2.4263434 27.910484 1
CTTGT 1869685 2.380076 92.38578 9
GACTC 1616955 2.3658128 30.88139 25-29
TGCAT 2003580 2.359231 23.81262 75-79
CTCAG 1611620 2.358007 31.131271 35-39
GAGAG 1600470 2.3517563 38.31265 95-97
ACAGG 1658090 2.338258 12.885817 90-94
CACTG 1583980 2.317566 30.519184 85-89
CAGCA 1702875 2.304659 15.636888 20-24
GGAAC 1632675 2.3024178 30.075058 70-74
CAGAC 1698475 2.298704 28.750547 25-29
GCACT 1570695 2.2981284 23.2951 85-89
TGAAG 2023380 2.296377 18.74366 60-64
GGAGG 1108210 2.2793155 16.637941 90-94
AAGGA 2162055 2.2697287 17.509779 70-74
TTCAC 2004370 2.2650678 19.323286 45-49
AAACT 2879580 2.2407677 33.457623 6
AAGTT 2555945 2.2404652 19.490791 60-64
TTTCA 2439955 2.2190473 18.741106 40-44
CTCAT 1948385 2.2018013 18.753683 30-34
TTGAA 2505750 2.1964657 17.939789 55-59
TCTCA 1937775 2.1898115 18.742842 35-39
CAGTA 2001545 2.180071 23.22203 35-39
CAGAA 2157120 2.173307 13.611832 6
ACCAG 1585145 2.145324 12.639489 55-59
AGGAA 2038340 2.1398525 22.628798 70-74
GGATT 1742480 2.1379232 20.118393 50-54
CTGAA 1951710 2.125791 22.883232 85-89
CATTC 1870350 2.1136167 24.403042 30-34
TAACC 2021090 2.112664 22.189775 10-14
TCAGT 1794090 2.1125548 25.411133 35-39
ATGCA 1934765 2.1073344 23.447645 75-79
GATGA 1849870 2.0994568 9.296282 15-19
GTTAC 1735500 2.043565 23.468412 60-64
GGCTC 982880 2.0128922 17.909021 65-69
TTAGC 1703300 2.0056493 25.29541 20-24
GCATA 1836005 1.9997659 17.78502 80-84
TAGAG 1758855 1.9961618 23.536598 90-94
AACCT 1887610 1.9731362 21.193392 10-14
ACTGA 1810825 1.9723397 23.316528 85-89
TTTCC 1614260 1.9721292 10.332159 40-44
AGTTT 2078400 1.9695833 20.614386 40-44
TGCTG 1192885 1.9660746 6.484746 1
CGTTA 1655515 1.9493817 19.194632 20-24
TCATT 2137030 1.9435484 19.716566 30-34
CAGGC 1013775 1.9204502 14.385455 65-69
GAAGA 1829255 1.9203547 8.584785 50-54
TGATG 1563940 1.9188647 11.440033 30-34
TTGTA 2004025 1.8991021 24.923485 9
CTGCA 1295185 1.8950219 17.316969 3
CATAG 1737235 1.8921859 23.059498 80-84
GTTAG 1539505 1.8888844 26.27677 20-24
GTAAC 1730835 1.8852152 23.26543 10-14
GTAGT 1535140 1.8835288 19.86029 40-44
TGTTG 1414850 1.8766928 10.619327 35-39
AAGAG 1786200 1.8751554 9.642567 50-54
CCATC 1333495 1.8724636 12.5685215 75-79
CCAGA 1368795 1.8525174 15.685392 55-59
TGAAT 2101740 1.8423225 18.01053 85-89
CCAAA 1899200 1.8363564 28.925488 4
TGACC 1251165 1.830615 42.75416 1
AGACT 1670555 1.8195585 23.303148 25-29
CTAAG 1670345 1.8193297 22.946436 65-69
TAAGG 1594445 1.8095696 22.612103 65-69
ACTCA 1730000 1.8083851 21.001905 25-29
GTAGG 1129695 1.794587 18.54032 7
AGGCT 1170560 1.7845821 13.178095 65-69
TGCAG 1159520 1.7677511 19.8336 4
GTTGT 1327090 1.7602859 11.580169 35-39
TTACT 1926695 1.7522566 14.960581 65-69
TGTAA 1989010 1.7435068 19.025986 10-14
ATTCT 1916635 1.7431076 19.60451 30-34
AAGAA 2318570 1.738961 9.262771 45-49
GCTGG 813390 1.7357186 5.8626037 1
TTAAA 2757930 1.7271589 13.858147 50-54
ACCAA 1782640 1.7236533 15.581848 3
TGTTT 1675480 1.7164967 11.803198 35-39
GGAGC 865660 1.7087138 18.292027 95-97
GAATA 2093900 1.6977903 13.289891 90-94
GGATG 1064835 1.6915532 14.384963 10-14
AATAG 2080580 1.6869901 17.436583 90-94
ATGAT 1919290 1.6823922 7.9829135 15-19
TTCCG 1040930 1.6465029 13.400528 40-44
AGGGA 1117595 1.6422122 17.222378 9
TTGTT 1602915 1.6421553 9.671586 35-39
CAGGA 1155735 1.6298311 12.134102 90-94
AGTTA 1857690 1.6283956 18.437239 60-64
CATCA 1557205 1.6277609 8.467292 80-84
GACCA 1197285 1.620397 19.463737 2
ATTAA 2585890 1.6194186 13.829165 50-54
TAGTT 1706260 1.616927 20.193035 40-44
AGCTG 1054030 1.6069258 7.616095 1
GATTA 1826025 1.600639 18.814188 50-54
TACCA 1527710 1.5969296 9.530595 75-79
TAAAC 2042960 1.5897453 15.8890085 50-54
GCTCA 1076335 1.5748162 15.950179 65-69
GAGGA 1071455 1.5744133 17.244976 95-97
TCCGT 981680 1.5527835 13.8069725 40-44
AAAGA 2068255 1.5512209 7.441369 45-49
AGAAA 2065170 1.5489072 6.5393267 60-64
GATGT 1261210 1.5474324 13.431023 30-34
AACAG 1519335 1.530736 12.974673 60-64
CAGAG 1076655 1.5183115 12.220143 25-29
ATACC 1446055 1.5115746 9.049378 75-79
GGAAG 1025940 1.5075327 8.291411 6
CTACA 1439890 1.5051304 9.766977 85-89
TAAAG 1843440 1.4947107 7.980124 45-49
ACTAA 1902325 1.480309 16.826332 65-69
CAAAC 1528950 1.4783578 33.423252 5
GAGAT 1299285 1.4745861 12.534068 25-29
TACAG 1353195 1.4738919 9.292277 90-94
GAGAA 1402765 1.4726248 11.845727 50-54
CTCAC 1048450 1.4722098 11.8049 65-69
TAGGG 921335 1.4635949 18.402077 8
TACTA 1737610 1.4617683 17.925854 65-69
GGGGG 496890 1.4304751 5.6669474 1
GAAAC 1416845 1.4274769 8.815163 60-64
GAGCT 934680 1.4249703 9.165037 95-97
ATAGA 1735260 1.4069955 17.26571 90-94
AGCCA 1038235 1.4051398 12.2693615 95-97
GAGCG 706305 1.3941652 5.145061 90-94
GTAAA 1706375 1.3835746 7.207772 45-49
ATGTT 1459365 1.3829585 8.283022 30-34
TCCTC 909975 1.3813698 7.5982294 1
CTCTT 1112690 1.3593649 5.461244 3
AGATG 1187080 1.3472422 9.754845 25-29
GCCAA 976040 1.3209655 13.82683 3
TTGAC 1118055 1.3165187 5.224854 6
GGGAT 824980 1.3105294 17.028118 10-14
AAACA 1812460 1.3046005 6.870693 60-64
TCTAC 1138610 1.2867032 12.007536 85-89
AGAAC 1267860 1.2773739 9.479867 50-54
CAACA 1312935 1.2694906 5.2050834 8
TGATA 1441195 1.2633085 8.143699 15-19
TCAAC 1197535 1.2517945 5.839124 7
CCGTA 855085 1.2510992 15.640556 40-44
GATAC 1139815 1.2414796 8.171365 75-79
CAATA 1557620 1.2120742 8.590137 80-84
GGCCA 622545 1.1793216 16.432348 2
ATCAA 1508135 1.173567 6.900047 80-84
AAGTA 1430635 1.1599973 18.851479 8
GGATA 1016175 1.1532787 9.514545 70-74
TCAAT 1353720 1.1388199 7.407571 80-84
CGTAA 1019845 1.1108091 9.159373 40-44
GTTGC 660945 1.0893483 5.4528465 1
CCTCT 713415 1.0829858 6.3366733 2
CCCAA 832565 1.0813881 6.6757336 3
TGGCC 522710 1.0704857 27.092098 1
GATAG 942375 1.0695214 12.3011265 15-19
CCGAA 786615 1.0645992 27.300268 4
GCCGA 556680 1.0545497 11.33556 3
GTCAA 940915 1.024839 5.8830395 6
CGGAA 720230 1.0156769 7.1255145 5
AATAT 1593280 0.9977946 5.473365 80-84
TATCT 1096390 0.9971255 7.6304526 85-89
ATCTA 1144645 0.96293515 7.5051417 85-89
CTAAA 1230510 0.9575309 5.628755 4
TGGCT 573240 0.94479567 5.577537 1
ATATC 1121605 0.9435527 6.415082 85-89
GGTGC 438440 0.93560094 6.9126463 2
GGCTG 426035 0.9091295 7.5836115 1
GGCCT 442710 0.9066494 5.8716645 2
CCTAA 864825 0.90400964 8.86354 4
ACCGA 654860 0.88628286 16.91307 3
GCCCA 482545 0.8772812 5.856749 2
ACCTA 811305 0.84806454 6.501906 3
CGAAC 625100 0.84600586 25.82537 5
GACCT 576415 0.8433691 8.9551115 2
GACCG 436970 0.8277765 23.236586 2
GAACT 757535 0.82510257 22.512136 6
GACTG 530350 0.8085473 6.484954 2
TGTCA 683215 0.8044911 5.344992 5
GTGCA 505065 0.76999897 5.442491 3
CTGTC 484795 0.76683 6.495603 4
GCTGT 455300 0.75041074 6.7037554 1
AAGTC 684550 0.74560785 5.0295095 6
TGACT 627525 0.73891556 9.730686 1
TGCGG 340335 0.7262516 9.959567 3
GGCCG 270615 0.717548 14.922017 2
TGTCC 449390 0.71082777 7.17237 1
GACTT 598525 0.70476794 6.9905844 6
GGGTG 303240 0.67425996 7.6334863 1
TGCCC 339460 0.667188 6.306163 1
GGGCC 245150 0.65002644 8.204081 1
GTCTA 551930 0.64990187 6.463197 8
GACCC 356085 0.64737314 5.341841 2
AGTCT 543190 0.6396104 6.3159437 7
CTAAC 598370 0.6254817 8.087801 5
GCGGA 306530 0.6050551 8.623729 4
TAACT 681840 0.5735994 6.778725 6
GGCCC 217710 0.55400926 5.7928443 1
Produced by FastQC (version 0.10.1)