FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | Undetermined_Undetermined_L002_R1_001.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 8790778 |
| Filtered Sequences | 0 |
| Sequence length | 101 |
| %GC | 44 |
Per base sequence quality
Per sequence quality scores
![[WARN]](Icons/warning.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
![[FAIL]](Icons/error.png)
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT | 183622 | 2.0888026065497276 | No Hit |
| CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT | 83734 | 0.9525209259066717 | No Hit |
| GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT | 75093 | 0.8542247341475351 | No Hit |
| ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG | 57528 | 0.6544130678763586 | No Hit |
| GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA | 56730 | 0.6453353730466177 | No Hit |
| GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT | 52327 | 0.5952487936790122 | No Hit |
| CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG | 47933 | 0.5452645943282836 | No Hit |
| GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC | 40270 | 0.4580936977364234 | No Hit |
| TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC | 39331 | 0.44741204930894624 | No Hit |
| TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC | 30020 | 0.3414942340712051 | No Hit |
| GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT | 25350 | 0.28837038086958855 | No Hit |
| GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA | 23186 | 0.2637536745894391 | No Hit |
| CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG | 21742 | 0.24732736965943172 | No Hit |
| TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT | 21634 | 0.24609880945691043 | No Hit |
| GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT | 21106 | 0.24009251513347282 | No Hit |
| ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG | 20525 | 0.23348331626620533 | No Hit |
| ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT | 19785 | 0.2250654037674481 | No Hit |
| CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA | 18982 | 0.21593083115055345 | No Hit |
| GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC | 18564 | 0.21117584814449872 | No Hit |
| GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT | 18480 | 0.21022030132031547 | No Hit |
| AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG | 18310 | 0.2082864565570874 | No Hit |
| CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC | 17874 | 0.20332671351727916 | No Hit |
| GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT | 17445 | 0.1984465993794861 | No Hit |
| GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT | 17366 | 0.1975479303424566 | No Hit |
| GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA | 16434 | 0.1869459108169948 | No Hit |
| GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC | 15776 | 0.17946079402755935 | No Hit |
| GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT | 15539 | 0.17676478691647088 | No Hit |
| ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG | 15371 | 0.17485369326810438 | No Hit |
| ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT | 14960 | 0.17017833916406488 | No Hit |
| AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC | 14777 | 0.1680966121542371 | No Hit |
| GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA | 13815 | 0.1571533259058527 | No Hit |
| GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG | 13653 | 0.15531048560207072 | No Hit |
| GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA | 13071 | 0.14868991117737246 | No Hit |
| TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC | 13035 | 0.14828039110986535 | No Hit |
| ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG | 12982 | 0.14767748656603547 | No Hit |
| TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC | 12973 | 0.14757510654915867 | No Hit |
| ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA | 12708 | 0.1445605838300091 | No Hit |
| GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA | 11600 | 0.1319564661967348 | No Hit |
| CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA | 11586 | 0.13179720839270426 | No Hit |
| TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA | 11420 | 0.12990886585919928 | No Hit |
| CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA | 11389 | 0.1295562235788459 | No Hit |
| ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG | 11348 | 0.12908982572418504 | No Hit |
| AACAAGTTTATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATT | 11082 | 0.12606392744760475 | No Hit |
| AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT | 11042 | 0.12560890515037462 | No Hit |
| TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA | 10554 | 0.12005763312416717 | No Hit |
| ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC | 10505 | 0.11950023081006027 | No Hit |
| ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC | 10496 | 0.11939785079318349 | No Hit |
| ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC | 10027 | 0.11406271435816034 | No Hit |
| CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA | 9898 | 0.1125952674495932 | No Hit |
| CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC | 9127 | 0.10382471267048263 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| AAAAA | 3749385 | 3.4542367 | 6.6077595 | 70-74 |
| CTGCT | 2449915 | 3.2907195 | 14.044932 | 3 |
| ACTTC | 2472065 | 2.9141228 | 6.80431 | 60-64 |
| AGCTG | 2139605 | 2.894925 | 6.8256364 | 80-84 |
| CAAGC | 1703280 | 2.8165824 | 8.585553 | 30-34 |
| CTTCT | 2637085 | 2.8017142 | 6.2200093 | 20-24 |
| CTGGT | 2233195 | 2.723216 | 13.277168 | 1 |
| AACTT | 2732010 | 2.5659845 | 5.5913033 | 60-64 |
| CAGCA | 1516590 | 2.5078678 | 6.703052 | 60-64 |
| GCTGC | 1461755 | 2.4823017 | 8.646105 | 3 |
| TCTCT | 2298195 | 2.4416678 | 8.069905 | 20-24 |
| TGGTG | 2199490 | 2.4349668 | 6.5008655 | 8 |
| GGAAG | 1783095 | 2.4302003 | 6.8644857 | 15-19 |
| AAGCT | 1994505 | 2.3683562 | 6.0897775 | 75-79 |
| CCAGC | 1139050 | 2.3640437 | 6.886946 | 60-64 |
| CTACT | 1988740 | 2.3443692 | 11.785513 | 2 |
| CTTTA | 2753465 | 2.330789 | 7.6861334 | 5 |
| CTGAA | 1948655 | 2.3139122 | 6.300964 | 80-84 |
| CTTCA | 1953840 | 2.3032281 | 6.686659 | 60-64 |
| TCAAC | 1750035 | 2.2889853 | 8.496857 | 8 |
| GCTGG | 1484350 | 2.2884 | 5.929545 | 75-79 |
| CAACT | 1741345 | 2.2776191 | 9.670095 | 9 |
| GCTAC | 1514315 | 2.2568588 | 14.713902 | 1 |
| TGCTA | 2108805 | 2.256836 | 10.374439 | 4 |
| GGTGT | 2035030 | 2.2528996 | 7.281285 | 9 |
| AGCAC | 1356370 | 2.2429242 | 5.58534 | 65-69 |
| CCAAG | 1347510 | 2.2282732 | 7.74469 | 75-79 |
| GCAGC | 1172535 | 2.2092972 | 6.0147543 | 1 |
| CAACA | 1522220 | 2.2091334 | 7.5280695 | 85-89 |
| TCAAG | 1858135 | 2.2064247 | 5.7339916 | 10-14 |
| AGGAA | 1844365 | 2.2060874 | 6.278266 | 40-44 |
| CGGTG | 1420260 | 2.1895933 | 7.647177 | 9 |
| GAAGC | 1444375 | 2.168363 | 7.5103245 | 15-19 |
| ACTGC | 1454125 | 2.1671546 | 14.242828 | 2 |
| TTCAA | 2304710 | 2.1646516 | 9.105801 | 9 |
| ACATC | 1652980 | 2.1620407 | 6.524205 | 90-94 |
| GCTTC | 1597570 | 2.1458519 | 8.380004 | 15-19 |
| TCCTT | 2003040 | 2.1280868 | 5.4986506 | 40-44 |
| TATGC | 1970355 | 2.1086674 | 6.7502184 | 30-34 |
| ACCAG | 1270555 | 2.1010187 | 5.828317 | 60-64 |
| ACAAC | 1438995 | 2.0883527 | 7.6506233 | 85-89 |
| ACCCA | 1127940 | 2.0545034 | 39.25384 | 1 |
| CTTGG | 1670955 | 2.037606 | 6.560911 | 6 |
| CATGG | 1464315 | 1.9812453 | 11.283175 | 2 |
| GCGGC | 920835 | 1.9769785 | 6.4151883 | 7 |
| TTGGT | 2248940 | 1.9692858 | 20.506304 | 7 |
| TGCTT | 2021845 | 1.9501283 | 5.1487694 | 4 |
| TTCCA | 1621000 | 1.9108694 | 6.006542 | 75-79 |
| CAGCT | 1270565 | 1.893586 | 5.3946104 | 2 |
| GCAAG | 1260275 | 1.8919836 | 6.012829 | 25-29 |
| AGAAA | 1766620 | 1.854501 | 7.9163637 | 9 |
| GTTCC | 1374080 | 1.8456607 | 5.8167458 | 70-74 |
| CAAGG | 1228545 | 1.8443489 | 7.0415263 | 10-14 |
| CCGCC | 706850 | 1.8412647 | 7.5093346 | 75-79 |
| CTCTG | 1352035 | 1.8160499 | 7.872042 | 20-24 |
| CATCC | 1105640 | 1.8150403 | 7.4116697 | 90-94 |
| GCCTT | 1351130 | 1.8148344 | 5.939323 | 35-39 |
| AAGGA | 1510635 | 1.8069052 | 6.223331 | 10-14 |
| GGTAT | 1855630 | 1.8028955 | 5.541053 | 6 |
| CGTGA | 1326345 | 1.7945693 | 9.010446 | 9 |
| CTCCT | 1204205 | 1.7816592 | 5.55312 | 70-74 |
| TCGGT | 1416370 | 1.7271584 | 7.8358326 | 8 |
| TTTCA | 2032625 | 1.7206031 | 8.251778 | 8 |
| TGGTA | 1765045 | 1.7148848 | 10.308971 | 2 |
| TTTAG | 2231295 | 1.7147312 | 6.582305 | 6 |
| TTCGG | 1400200 | 1.7074403 | 6.180128 | 7 |
| AGCTT | 1592515 | 1.7043042 | 5.3972616 | 15-19 |
| CTTTC | 1597625 | 1.6973623 | 10.167979 | 7 |
| GCACC | 813640 | 1.6886709 | 6.835101 | 50-54 |
| AAACC | 1160540 | 1.6842428 | 5.031934 | 70-74 |
| AAAGC | 1272280 | 1.6762658 | 5.160531 | 20-24 |
| AACAT | 1605985 | 1.6736379 | 5.2087297 | 90-94 |
| GTCAA | 1391150 | 1.6519078 | 5.751963 | 10-14 |
| ACCGC | 795875 | 1.6518004 | 6.1109114 | 75-79 |
| TCCAA | 1261660 | 1.6502078 | 6.1157618 | 75-79 |
| TCTGG | 1352245 | 1.6489627 | 5.631774 | 45-49 |
| CCTGT | 1226405 | 1.647304 | 5.3506303 | 1 |
| CCTTA | 1395840 | 1.645446 | 8.592772 | 95-97 |
| CATGA | 1373810 | 1.6313175 | 5.402702 | 65-69 |
| CACAA | 1123420 | 1.6303719 | 6.53909 | 85-89 |
| GCTGA | 1203970 | 1.6289936 | 6.319456 | 80-84 |
| GACGG | 951710 | 1.627978 | 9.626869 | 3 |
| GGTCG | 1047835 | 1.6154314 | 10.114096 | 6 |
| GGCTC | 947470 | 1.6089607 | 5.4216166 | 90-94 |
| ATCGG | 1187295 | 1.6064321 | 5.412491 | 85-89 |
| TACTT | 1869750 | 1.5827305 | 10.160276 | 3 |
| GAACA | 1187530 | 1.5646054 | 6.202898 | 80-84 |
| CTGCG | 914180 | 1.5524288 | 6.030903 | 45-49 |
| ACGGT | 1146235 | 1.5508771 | 7.6502495 | 4 |
| CCAAT | 1181715 | 1.5456424 | 28.396679 | 3 |
| ATGCC | 1034805 | 1.5422212 | 7.932865 | 9 |
| TGGTC | 1264105 | 1.5414824 | 24.293007 | 8 |
| CCCAA | 846155 | 1.5412416 | 39.098244 | 2 |
| TCGGA | 1132365 | 1.5321108 | 5.6400766 | 85-89 |
| GGTCA | 1119560 | 1.5147854 | 26.770193 | 9 |
| TGACG | 1115065 | 1.5087036 | 7.691363 | 2 |
| CTATG | 1409610 | 1.5085598 | 5.0447183 | 35-39 |
| TCATG | 1394215 | 1.4920841 | 5.0200663 | 60-64 |
| ACTTT | 1762515 | 1.4919568 | 10.454665 | 4 |
| TGAAC | 1250160 | 1.4844909 | 5.831574 | 80-84 |
| AAGCC | 893420 | 1.4773797 | 5.071099 | 30-34 |
| CCACA | 810495 | 1.4762883 | 6.005317 | 60-64 |
| GTGTA | 1515015 | 1.4719603 | 6.2438493 | 2 |
| CATGC | 986670 | 1.4704832 | 5.664233 | 2 |
| TTAGG | 1511115 | 1.4681712 | 5.848529 | 35-39 |
| GGTAC | 1075995 | 1.4558411 | 12.854879 | 3 |
| GGGTC | 939915 | 1.4490528 | 9.978118 | 5 |
| GTGTT | 1639220 | 1.435384 | 5.0605135 | 70-74 |
| AACAC | 988630 | 1.4347571 | 6.5189977 | 85-89 |
| ATGGG | 1159195 | 1.4238878 | 9.389571 | 3 |
| CTGTA | 1329240 | 1.4225482 | 5.6739492 | 8 |
| GCGAC | 754290 | 1.4212375 | 5.504699 | 50-54 |
| GACGC | 751085 | 1.4151987 | 5.8237624 | 15-19 |
| CTCTA | 1185930 | 1.3979995 | 6.0786624 | 95-97 |
| GACTG | 1031730 | 1.3959497 | 11.844945 | 1 |
| CACCC | 608130 | 1.390252 | 7.0663066 | 50-54 |
| GCAAC | 831895 | 1.3756405 | 6.701099 | 1 |
| ATCTC | 1156445 | 1.363242 | 5.7877545 | 45-49 |
| ACACA | 930655 | 1.3506203 | 6.3022485 | 85-89 |
| CTGAC | 905430 | 1.3494072 | 8.444038 | 1 |
| CGCAA | 809920 | 1.339302 | 5.199665 | 25-29 |
| TTCTC | 1225870 | 1.3023992 | 5.2733064 | 20-24 |
| GTCGC | 762745 | 1.2952671 | 5.148825 | 40-44 |
| TGGGT | 1164855 | 1.289564 | 7.613679 | 4 |
| GCGCA | 682715 | 1.2863755 | 5.714866 | 25-29 |
| AGCGC | 680840 | 1.2828426 | 5.4708924 | 25-29 |
| GTATG | 1312250 | 1.2749577 | 5.536865 | 7 |
| CGCCT | 674985 | 1.262579 | 5.6454177 | 75-79 |
| GAATC | 1051420 | 1.2484989 | 5.3278985 | 40-44 |
| ACATG | 1048100 | 1.2445563 | 10.732819 | 1 |
| ATCCT | 1052625 | 1.2408568 | 5.265205 | 90-94 |
| AACCG | 728215 | 1.2041929 | 5.5395966 | 70-74 |
| TCTGA | 1118820 | 1.1973574 | 5.076055 | 20-24 |
| CGGTA | 879390 | 1.1898309 | 7.346987 | 5 |
| CTTAT | 1398955 | 1.1842058 | 6.1393847 | 95-97 |
| TGCCT | 880080 | 1.1821212 | 5.768564 | 30-34 |
| ACGCG | 611645 | 1.152465 | 6.242298 | 15-19 |
| CAATT | 1218835 | 1.1447657 | 20.58656 | 4 |
| TTATG | 1482535 | 1.1393155 | 5.689326 | 95-97 |
| ATTGG | 1168240 | 1.1350403 | 19.750122 | 6 |
| GTACT | 1058260 | 1.1325463 | 10.139693 | 4 |
| TGGCC | 634785 | 1.0779699 | 6.925768 | 1 |
| CGCGA | 567865 | 1.0699744 | 5.255174 | 20-24 |
| TCGTG | 855400 | 1.043097 | 7.9888463 | 8 |
| TTAGA | 1090425 | 0.92978626 | 6.4258165 | 7 |
| TAGAA | 982755 | 0.9297815 | 6.540284 | 8 |
| AATTG | 1079680 | 0.9206242 | 16.952765 | 5 |
| GTCGT | 631700 | 0.7703114 | 7.8259034 | 7 |
| TGACT | 649565 | 0.6951623 | 5.2228637 | 1 |