##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-99_TAAGGCGA-TATCCTCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3475656	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.593496306884226	31.0	31.0	34.0	30.0	34.0
2	31.78932984161839	33.0	31.0	34.0	30.0	34.0
3	31.929489857454247	34.0	31.0	34.0	30.0	34.0
4	35.45959841825543	37.0	35.0	37.0	33.0	37.0
5	35.32698172661506	37.0	35.0	37.0	33.0	37.0
6	35.471608525124466	37.0	35.0	37.0	33.0	37.0
7	35.48652685996543	37.0	35.0	37.0	33.0	37.0
8	35.53755722660701	37.0	35.0	37.0	33.0	37.0
9	37.325188971520774	39.0	37.0	39.0	34.0	39.0
10-14	37.44358814566229	39.2	37.2	39.4	34.0	39.4
15-19	38.484635648637266	40.0	38.0	41.0	34.0	41.0
20-24	38.37909764372538	40.0	38.0	41.0	34.0	41.0
25-29	38.193804104894156	40.0	38.0	41.0	33.2	41.0
30-34	37.92849200266079	40.0	38.0	41.0	32.8	41.0
35-39	37.61196637411758	40.0	37.4	41.0	31.8	41.0
40-44	37.56481728916786	40.0	37.0	41.0	31.8	41.0
45-49	37.392951661499296	40.0	37.0	41.0	31.2	41.0
50-54	36.71429399227081	38.8	36.0	40.2	30.6	40.6
55-59	37.33757046151864	40.0	36.2	41.0	31.4	41.0
60-64	36.882552300918164	39.0	35.2	41.0	31.0	41.0
65-69	36.22016580467111	38.0	35.0	40.2	30.2	41.0
70-74	35.39991299484184	36.6	34.4	39.2	29.2	41.0
75-79	34.46626887125768	35.4	34.0	37.8	29.0	39.4
80-84	33.57054950202207	35.0	33.6	36.4	27.6	38.2
85-89	32.8172998708733	35.0	33.0	35.4	26.2	36.8
90-94	32.262541920143995	35.0	32.6	35.0	25.4	36.0
95-99	31.799866845280427	35.0	32.0	35.0	24.4	35.2
100-101	30.966044107932433	34.5	30.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	3.0
6	85.0
7	339.0
8	731.0
9	1088.0
10	1249.0
11	1728.0
12	2755.0
13	3891.0
14	4896.0
15	5870.0
16	6690.0
17	7614.0
18	8448.0
19	9737.0
20	11280.0
21	12660.0
22	14563.0
23	16471.0
24	19279.0
25	22073.0
26	25275.0
27	30197.0
28	36456.0
29	43452.0
30	54235.0
31	67983.0
32	88623.0
33	118765.0
34	169100.0
35	260769.0
36	448260.0
37	808735.0
38	974438.0
39	197776.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80545685998718	23.382661450360036	10.613416821869345	24.198464867783436
2	15.276825218294016	23.515055300575007	39.91700236893509	21.291117112195888
3	15.83326857935528	27.47401926502796	32.78496786763711	23.907744287979646
4	11.361553798619521	18.53409870562828	43.996608663219305	26.10773883253289
5	10.869203577824122	38.92953086388485	38.589828678197875	11.611436880093159
6	25.453907239037864	43.320376337898495	19.716192887558982	11.50952353550466
7	24.130424587406093	33.82756464793608	26.419878048113826	15.622132716544007
8	21.234512203648393	38.38545108858747	24.22447727113825	16.15555943662588
9	22.735620430521777	15.582095169152874	25.323832165731634	36.35845223459371
10-14	20.454887598808543	25.991077882078883	32.8004309134352	20.753603605677377
15-19	19.774487393516647	31.766139380846138	28.649102879298866	19.81027034633835
20-24	19.615985616007485	30.10638352977605	30.40802031900095	19.869610535215514
25-29	19.66502960600404	30.615879001218442	29.846401410895933	19.872689981881578
30-34	19.523145080190137	30.365000516746854	29.936935016723492	20.174919386339514
35-39	19.70859949547834	30.648183038376835	29.812049684265386	19.831167781879437
40-44	19.44982304012558	30.441397440016743	30.215338156890553	19.893441362967128
45-49	19.6322200137862	30.272284854255695	30.218811263430407	19.876683868527707
50-54	19.42113600085997	29.96414412189295	30.493264046189793	20.121455831057286
55-59	19.595848736585637	29.99520703519074	30.531099516715614	19.877844711508
60-64	19.334212184789923	29.658140518437953	30.769845216521862	20.237802080250262
65-69	19.656472316666928	29.756114152563846	30.369719231894138	20.217694298875095
70-74	19.634181378850553	29.57229535852545	30.36736680761878	20.426156455005213
75-79	19.864864289388294	29.989540137822036	29.929633026646524	20.215962546143153
80-84	19.951931294312313	29.910554431999582	29.754981158904346	20.38253311478376
85-89	20.05396331392411	29.898213806827794	29.662972430771923	20.384850448476172
90-94	20.117183903177448	29.561939240977214	30.012928994464232	20.307947861381106
95-99	20.450224708360814	29.550290354405195	29.51478843034363	20.48469650689036
100-101	20.615161731902045	28.95212171740023	30.08232800137537	20.35038854932235
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.00392172777062
2	36.5679423304899
3	39.74101286733493
4	37.46929263115241
5	22.480640457917282
6	36.96343077454253
7	39.7525573039501
8	37.39007164027427
9	59.09407266511549
10-14	41.20849120448592
15-19	39.584757739855
20-24	39.485596151223
25-29	39.537719587885626
30-34	39.69806446652965
35-39	39.53976727735778
40-44	39.34326440309271
45-49	39.50890388231391
50-54	39.54259183191726
55-59	39.47369344809364
60-64	39.57201426504018
65-69	39.87416661554202
70-74	40.06033783385576
75-79	40.08082683553144
80-84	40.33446440909607
85-89	40.43881376240029
90-94	40.42513176455856
95-99	40.93492121525117
100-101	40.965550281224395
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	7.0
2	6.5
3	12.5
4	23.0
5	31.5
6	50.0
7	67.5
8	82.5
9	105.5
10	148.0
11	201.0
12	270.5
13	384.5
14	553.0
15	815.0
16	1173.0
17	1622.5
18	2302.5
19	3220.5
20	4560.0
21	6771.5
22	9785.0
23	13625.5
24	18252.0
25	24135.0
26	31373.0
27	39652.5
28	49342.0
29	59929.0
30	71220.5
31	83540.0
32	96073.5
33	109595.5
34	125684.0
35	144153.5
36	166020.5
37	186291.5
38	198888.0
39	201691.0
40	194447.5
41	181656.0
42	166340.5
43	151571.0
44	139254.0
45	126724.0
46	114585.0
47	103218.0
48	91597.5
49	78926.5
50	67603.5
51	60755.5
52	54052.5
53	47017.5
54	40976.5
55	35545.5
56	31641.5
57	27327.0
58	22370.0
59	18619.0
60	15520.5
61	12747.5
62	10129.5
63	6918.0
64	4734.0
65	3924.5
66	2938.5
67	2027.0
68	1513.0
69	1644.0
70	1780.0
71	1412.5
72	1142.5
73	953.0
74	702.5
75	490.5
76	322.5
77	230.5
78	138.0
79	70.5
80	54.0
81	48.5
82	44.5
83	37.0
84	36.5
85	32.5
86	23.0
87	20.0
88	21.0
89	18.0
90	13.0
91	13.5
92	14.0
93	10.0
94	7.0
95	8.0
96	8.0
97	7.5
98	5.5
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033576395362486966
2	0.03161993016570109
3	0.027793314413164016
4	0.0264698232506324
5	0.032857106687198046
6	0.0016112066326471894
7	6.617455812658099E-4
8	0.0027908400601210246
9	3.740301111502404E-4
10-14	0.0071353436588661254
15-19	0.02350635390844203
20-24	0.019622195061881842
25-29	0.002221163429292197
30-34	0.0019104307215673819
35-39	0.00591543006557611
40-44	0.013275191791132379
45-49	0.008504869296616237
50-54	0.0053745249817588395
55-59	0.004183382935480381
60-64	0.010432562946390552
65-69	0.014075040798053662
70-74	0.01790741085999305
75-79	0.007929438356385097
80-84	0.010944696483196265
85-89	0.00955215360783691
90-94	0.010541894825034469
95-99	0.010110321619861114
100-101	0.006574298492140764
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3475656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.497348595508107
#Duplication Level	Relative count
1	100.0
2	9.634502830867602
3	2.8499735700775064
4	1.7558384654086447
5	1.3775402976709825
6	1.1119673415319165
7	0.9247288544698412
8	0.7941714060081901
9	0.722842458555971
10++	8.2015552258007
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	391390	2.385445	5.192059	95-97
ACACA	821525	2.252024	7.3327312	6
CTTTG	815950	2.2125309	8.889462	9
CGACG	327885	2.0158606	5.172827	95-97
TACAC	725880	1.9725949	9.207608	5
GACGC	320010	1.9674442	5.1797843	95-97
CGCTG	319610	1.9479601	5.112242	95-97
CACAT	694085	1.886191	5.0144997	7
GCTTT	691315	1.8745706	5.6626143	1
GAGAG	405895	1.7178359	5.473825	7
TGGCT	399385	1.6360255	5.083442	6
TTGAG	550930	1.5300748	5.0048947	9
GACTC	364180	1.4821168	5.3959103	7
GAGTC	352350	1.455968	7.606883	9
CCATG	350090	1.4247743	5.4137125	9
AAGAC	506415	1.4095159	5.6110783	5
GTCTT	508155	1.3779136	6.7583065	1
GTGTA	485105	1.3472617	10.148507	1
GACTT	490435	1.3414869	6.794296	7
TATAC	723730	1.3218685	5.86335	5
TGAGT	462830	1.2853982	5.39826	8
AACAC	467615	1.2818604	5.1646256	5
GTGTT	463405	1.2758445	6.37584	1
TCATA	696695	1.27249	5.970518	2
TATGA	684460	1.2693195	5.82915	4
GATTG	444880	1.2355465	5.6715446	7
GTTCT	446660	1.2111639	5.2890525	1
ACACC	299415	1.2106203	5.127378	6
ACATG	410065	1.1314541	5.0687876	8
TGGAC	272615	1.12649	7.334109	5
GTCCA	274750	1.1181602	8.10199	1
ACACT	411215	1.1174856	5.5019774	6
GGACT	255730	1.0567183	7.165794	6
AGACT	373335	1.0301085	5.4967246	6
GAGTA	350095	0.98080194	5.1206408	1
GTATA	408955	0.7584002	7.148981	1
>>END_MODULE