##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-99_TAAGGCGA-TATCCTCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3475656	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.457192829209795	34.0	31.0	34.0	31.0	34.0
2	32.63954200300605	34.0	31.0	34.0	31.0	34.0
3	32.74780847126413	34.0	31.0	34.0	31.0	34.0
4	36.257879663580056	37.0	37.0	37.0	35.0	37.0
5	35.98477179559772	37.0	35.0	37.0	35.0	37.0
6	36.07775050235121	37.0	36.0	37.0	35.0	37.0
7	36.08039345665969	37.0	36.0	37.0	35.0	37.0
8	36.10397432887489	37.0	36.0	37.0	35.0	37.0
9	37.9649631609112	39.0	38.0	39.0	35.0	39.0
10-14	38.15187671046847	39.2	38.2	39.4	35.2	39.4
15-19	39.29019413889061	40.0	39.0	41.0	36.0	41.0
20-24	39.22148181523143	40.0	39.0	41.0	36.0	41.0
25-29	39.03208746780464	40.0	39.0	41.0	36.0	41.0
30-34	38.837368542801705	40.0	38.0	41.0	35.0	41.0
35-39	38.61168050002647	40.0	38.0	41.0	34.8	41.0
40-44	38.37223562976313	40.0	38.0	41.0	34.0	41.0
45-49	38.19391015681644	40.0	38.0	41.0	33.4	41.0
50-54	38.133610000529394	40.0	37.4	41.0	33.4	41.0
55-59	37.74746925472486	39.8	36.6	41.0	33.0	41.0
60-64	37.1945324853783	39.0	35.4	40.8	32.0	41.0
65-69	36.468634295223694	37.8	35.0	40.0	31.0	41.0
70-74	35.90201469880794	36.6	35.0	39.2	31.0	41.0
75-79	34.73099932789666	35.2	33.8	37.4	30.4	39.2
80-84	34.536619446803705	35.0	34.0	36.6	31.0	38.4
85-89	33.903951944611315	35.0	34.0	35.6	30.4	36.8
90-94	33.48506837270431	35.0	34.0	35.0	30.0	36.0
95-99	33.20372016102859	35.0	34.0	35.0	29.2	35.6
100-101	32.85596416906621	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	12.0
14	32.0
15	93.0
16	239.0
17	535.0
18	965.0
19	1685.0
20	2408.0
21	3615.0
22	5215.0
23	7299.0
24	9850.0
25	12820.0
26	16868.0
27	21866.0
28	28186.0
29	35701.0
30	44978.0
31	57623.0
32	74900.0
33	101709.0
34	147154.0
35	236563.0
36	439665.0
37	850905.0
38	1131030.0
39	243489.0
40	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38956789739836	23.41816911685161	10.936899393956134	24.255363591793895
2	15.528933144259414	23.3629931537438	39.75516116556706	21.352912536429724
3	16.05412043079062	27.16580697284196	33.01330741592379	23.766765180443635
4	11.607535383248514	18.46865742754749	43.72342947633483	26.200377712869166
5	10.72079630435233	38.941943621578204	38.74482975300202	11.592430321067447
6	25.39322073300695	43.49023608780615	19.602256379802835	11.51428679938406
7	24.057242719072313	33.81819144357209	26.397692982274425	15.72687285508117
8	21.185497068754792	38.51992257001268	24.11938926061728	16.17519110061525
9	22.603502763219375	15.535455752813283	25.310358677613664	36.55068280635368
10-14	20.401725602303568	25.93544930798675	32.81151529380353	20.851309795906154
15-19	19.68863553539914	31.782576118401035	28.6870289722847	19.84175937391512
20-24	19.55419893538756	30.08922984056708	30.458526811390506	19.89804441265485
25-29	19.613925617320916	30.632825485011654	29.839982858327797	19.913266039339632
30-34	19.48020902497787	30.343558652204845	29.980211844787107	20.196020478030174
35-39	19.679135685150868	30.611736183219257	29.827997213930324	19.88113091769955
40-44	19.428182334349188	30.394838518906774	30.248390290028176	19.928588856715866
45-49	19.597888864145258	30.209045560343846	30.248580996230807	19.944484579280093
50-54	19.375036702941646	29.924014835472946	30.54018669850851	20.1607617630769
55-59	19.524311100618487	29.964959279964987	30.5461658277077	19.964563791708827
60-64	19.204054722144644	29.621987654660032	30.759336916847698	20.41462070634763
65-69	19.490856751786577	29.749965343805567	30.30538554958737	20.45379235482048
70-74	19.383399567040502	29.58740521637991	30.294332240336658	20.734862976242933
75-79	19.572933667924904	29.970958112966144	29.816797706848135	20.639310512260817
80-84	19.493893571052816	29.93142229231242	29.613599580440876	20.96108455619389
85-89	19.561460321936046	29.861911907087684	29.519466787127733	21.05716098384853
90-94	19.42155525395756	29.497580390618953	29.938959494596702	21.141904860826784
95-99	19.613338180109842	29.465101971915665	29.505273746677744	21.41628610129675
100-101	19.44613876985454	28.80141406480622	30.31652460227629	21.435922563062952
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.64493148919226
2	36.88184568068914
3	39.82088561123425
4	37.80791309611769
5	22.313226625419777
6	36.90750753239101
7	39.78411557415348
8	37.36068816937004
9	59.154185569573045
10-14	41.25303539820972
15-19	39.53039490931427
20-24	39.452243348042416
25-29	39.52719165666055
30-34	39.676229503008045
35-39	39.56026660285042
40-44	39.35677119106506
45-49	39.54237344342535
50-54	39.53579846601855
55-59	39.48887489232732
60-64	39.618675428492274
65-69	39.94464910660706
70-74	40.118262543283436
75-79	40.21224418018572
80-84	40.454978127246704
85-89	40.61862130578457
90-94	40.56346011478434
95-99	41.02962428140659
100-101	40.88206133291749
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	6.0
7	13.5
8	21.0
9	40.5
10	67.0
11	99.5
12	166.5
13	289.5
14	487.5
15	720.5
16	1076.0
17	1582.0
18	2294.0
19	3304.0
20	4784.0
21	6975.5
22	10046.0
23	13913.0
24	18498.5
25	24253.0
26	31455.5
27	39745.5
28	49173.0
29	59593.0
30	70790.0
31	82829.5
32	95232.5
33	108571.0
34	123815.5
35	142175.5
36	164787.0
37	185774.5
38	197866.5
39	200253.0
40	194021.0
41	181858.5
42	165773.5
43	150251.0
44	138757.5
45	127264.0
46	116007.5
47	105492.0
48	93718.0
49	80624.0
50	69377.5
51	62497.5
52	55453.0
53	48002.0
54	41775.0
55	36229.5
56	32111.5
57	27446.0
58	22203.0
59	18464.0
60	15363.0
61	12763.5
62	10120.0
63	6624.5
64	4508.0
65	3765.0
66	2784.0
67	1845.5
68	1310.5
69	1453.5
70	1667.5
71	1339.5
72	1148.0
73	1006.5
74	745.5
75	554.5
76	329.0
77	181.0
78	67.0
79	17.0
80	11.0
81	9.0
82	5.5
83	3.0
84	3.0
85	1.0
86	1.0
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013580170189454885
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010875644770368528
20-24	7.192886752889238E-4
25-29	0.017717518649716775
30-34	0.007008748852015274
35-39	0.043577385103704167
40-44	0.051800293239607144
45-49	0.052088008709722716
50-54	0.05236421556103366
55-59	0.04227691117878179
60-64	0.053497814513289006
65-69	0.04400895830887752
70-74	0.003872650227755566
75-79	0.0015939437044402555
80-84	2.1866375728783286E-4
85-89	0.0034813571883983915
90-94	0.0027332969660979105
95-99	0.0023420039267407363
100-101	5.7543094023113915E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3475656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.304340162254817
#Duplication Level	Relative count
1	100.0
2	9.972480418759503
3	2.896933074174573
4	1.7801127725496988
5	1.4561643220496636
6	1.1476114414743823
7	0.9346393908486167
8	0.8518882025030631
9	0.7319310535060203
10++	8.524655363752414
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	938100	2.722447	5.028881	5
CCGAG	413860	2.539744	5.7299876	95-97
CTCCG	416435	2.4321432	5.42309	95-97
GAGCC	395250	2.4255397	5.5316157	95-97
CGAGC	367565	2.2556443	5.3530803	95-97
GCCCA	374790	2.210343	5.0033174	90-94
ACACA	824035	2.208646	7.2487903	6
CTTTG	814230	2.2054815	8.789895	9
TACAC	728030	1.9324139	9.327723	5
GCTTT	690460	1.8702292	5.4390273	1
GAGAG	400135	1.7570934	5.6144214	7
TTGAG	550435	1.566596	5.1968217	9
GAGTC	355010	1.4836597	7.3635154	9
GACTC	368415	1.4796743	5.314213	7
AGACT	528985	1.4610298	5.6417484	6
CCATG	354130	1.4223009	5.608256	9
GTCTT	524670	1.4211588	6.336119	1
AAGAC	508430	1.418001	5.8389473	5
GACTT	492370	1.346721	6.439784	7
TGAGT	469395	1.3359476	5.467288	8
GTGTT	468495	1.320463	6.434538	1
TATAC	728395	1.3166642	5.8098197	5
TATGA	690085	1.2980026	5.9916286	4
TCATA	699025	1.2635742	5.905195	2
GATTG	442520	1.2594585	5.9626837	7
AACAC	463085	1.2411985	5.176037	5
GTGTA	430260	1.2245653	10.637813	1
GTTCT	447755	1.2128212	5.104096	1
ACACC	300180	1.1699717	5.232667	6
TGGAC	271250	1.1336095	7.1629133	5
ACATG	410165	1.1328549	5.025749	8
GTATG	393725	1.1205828	5.23546	3
GTCCA	277095	1.1129035	7.7415094	1
ACACT	412475	1.0948343	5.6432195	6
GGACT	256290	1.0710887	6.911654	6
GAGTA	352195	1.0121942	5.117325	1
GTATA	411760	0.7744923	7.115131	1
>>END_MODULE