##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-98_TAAGGCGA-CTCTCTAT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1517524	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.670089566952484	33.0	31.0	34.0	30.0	34.0
2	31.865731283327314	33.0	31.0	34.0	30.0	34.0
3	32.00468394569048	34.0	31.0	34.0	30.0	34.0
4	35.53361726074843	37.0	35.0	37.0	33.0	37.0
5	35.408226822112866	37.0	35.0	37.0	33.0	37.0
6	35.55712792680709	37.0	35.0	37.0	33.0	37.0
7	35.570387025180494	37.0	35.0	37.0	33.0	37.0
8	35.62161389210319	37.0	35.0	37.0	33.0	37.0
9	37.41719867362888	39.0	37.0	39.0	35.0	39.0
10-14	37.541974822144496	39.2	37.2	39.4	34.0	39.4
15-19	38.59858308665959	40.0	38.0	41.0	34.0	41.0
20-24	38.49864924706298	40.0	38.0	41.0	34.0	41.0
25-29	38.321420287257396	40.0	38.0	41.0	33.8	41.0
30-34	38.06428550718143	40.0	38.0	41.0	33.0	41.0
35-39	37.75716996897578	40.0	37.8	41.0	32.4	41.0
40-44	37.72590627891223	40.0	37.8	41.0	32.6	41.0
45-49	37.56462263529276	40.0	37.0	41.0	31.8	41.0
50-54	36.88031385335586	39.2	36.4	40.2	31.2	40.6
55-59	37.513378503404226	40.0	36.6	41.0	32.0	41.0
60-64	37.07040679422533	39.0	35.6	41.0	31.0	41.0
65-69	36.410339210450715	38.2	35.0	40.2	30.8	41.0
70-74	35.5829544705718	36.6	34.8	39.4	30.0	41.0
75-79	34.632438103120606	35.6	34.0	38.0	29.0	39.4
80-84	33.72395520598027	35.0	34.0	36.4	28.6	38.2
85-89	32.963042166054706	35.0	33.0	35.4	26.8	36.6
90-94	32.39578972062386	35.0	33.0	35.0	25.6	36.0
95-99	31.920808633010086	35.0	32.0	35.0	24.8	35.2
100-101	31.061443509295405	34.5	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	43.0
7	181.0
8	298.0
9	466.0
10	515.0
11	725.0
12	1105.0
13	1442.0
14	1801.0
15	2153.0
16	2571.0
17	2856.0
18	3275.0
19	3887.0
20	4372.0
21	4869.0
22	5754.0
23	6514.0
24	7556.0
25	8712.0
26	10206.0
27	12311.0
28	14733.0
29	17963.0
30	22276.0
31	28389.0
32	36586.0
33	50424.0
34	71940.0
35	111672.0
36	194609.0
37	360219.0
38	440113.0
39	86924.0
40	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.387068259167435	23.536100544024595	10.866583126074882	24.210248070733083
2	15.11363471570557	23.30338933221889	40.31010102474929	21.272874927326253
3	15.855551578693422	27.1983753309749	32.69824383086515	24.247829259466524
4	11.105178857447944	18.361972935740507	44.275968440410516	26.25687976640103
5	10.60574699611611	39.309746110178004	39.12853749021117	10.95596940349471
6	25.57135128260381	43.72279652243593	19.490029317976486	11.215822876983774
7	24.344006320893413	33.79859203285388	26.268939780837915	15.588461865414788
8	21.162544877506154	38.868862786860184	23.963204398918194	16.00538793671547
9	22.838836625116308	15.089923269342794	25.065699472031927	37.00554063350897
10-14	20.464973808664265	26.000985099480683	32.76275591135475	20.7712851805003
15-19	19.699963378336584	31.90964968966502	28.597261100332023	19.793125831666377
20-24	19.643762005674667	30.119762783950847	30.415979655143506	19.82049555523098
25-29	19.65659036512974	30.75912297164788	29.746264879854074	19.838021783368305
30-34	19.479267752154847	30.361014156174864	29.93458523708486	20.225132854585432
35-39	19.719148749965072	30.69998107325704	29.747132914765867	19.833737262012026
40-44	19.356894967033316	30.4051525591375	30.28533602474574	19.952616449083443
45-49	19.61491640411147	30.28024604269211	30.136091111926543	19.96874644126988
50-54	19.38004082414316	29.789683303816428	30.64249061277996	20.18778525926046
55-59	19.620984068071827	29.809892346137218	30.65941944939425	19.909704136396705
60-64	19.205351172684534	29.424437607151244	31.03683847639519	20.333372743769033
65-69	19.614528133812538	29.620217191234843	30.426544857215994	20.338709817736625
70-74	19.464323423825057	29.374829130121054	30.515060144879204	20.64578730117469
75-79	19.79741623319408	29.85332118094342	30.00069197447998	20.34857061138252
80-84	19.869535015836902	29.739847707372498	29.788129505411437	20.60248777137916
85-89	20.14598886498798	29.782295225441814	29.56198068244127	20.50973522712894
90-94	20.083985940744554	29.357975079170966	30.045674390575357	20.512364589509126
95-99	20.585270798264418	29.251242314495073	29.428509629889728	20.73497725735078
100-101	20.70662254339698	28.429589654250933	30.247120844620344	20.61666695773175
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.59731632990052
2	36.38650964303182
3	40.10338083815995
4	37.362058623848974
5	21.56171639961082
6	36.78717415958758
7	39.9324681863082
8	37.16793281422162
9	59.84437725862528
10-14	41.23625898916457
15-19	39.493089210002964
20-24	39.464257560905644
25-29	39.49461214849804
30-34	39.70440060674028
35-39	39.552886011977094
40-44	39.30951141611676
45-49	39.58366284538135
50-54	39.56782608340362
55-59	39.53068820446853
60-64	39.53872391645356
65-69	39.953237951549156
70-74	40.11011072499974
75-79	40.145986844576605
80-84	40.47202278721607
85-89	40.65572409211692
90-94	40.59635053025368
95-99	41.3202480556152
100-101	41.32328950112873
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	4.0
2	3.5
3	5.0
4	7.0
5	10.5
6	16.5
7	25.5
8	32.0
9	46.5
10	61.0
11	79.5
12	115.0
13	159.0
14	215.0
15	280.5
16	376.5
17	556.0
18	816.5
19	1179.5
20	1698.0
21	2522.0
22	3618.5
23	5088.0
24	7038.0
25	9499.5
26	12622.0
27	16209.0
28	20401.5
29	24774.5
30	29640.5
31	34967.0
32	40470.0
33	46922.0
34	54587.5
35	63742.0
36	75019.0
37	85747.5
38	92310.0
39	93313.5
40	89203.5
41	82220.5
42	74177.0
43	66584.0
44	60497.5
45	54883.5
46	49203.0
47	43920.5
48	38664.5
49	33168.0
50	28258.5
51	25119.0
52	22125.5
53	19420.5
54	17133.5
55	14588.0
56	12670.5
57	11006.0
58	9216.0
59	7822.5
60	6637.5
61	5507.0
62	4486.0
63	3249.5
64	2384.0
65	1978.0
66	1548.0
67	1174.5
68	961.5
69	945.5
70	937.0
71	801.5
72	680.0
73	548.5
74	428.0
75	325.5
76	225.0
77	150.0
78	102.0
79	74.0
80	51.0
81	43.5
82	37.5
83	30.5
84	24.0
85	17.5
86	15.0
87	14.5
88	14.5
89	11.5
90	8.0
91	7.0
92	4.5
93	5.0
94	5.5
95	5.0
96	4.5
97	6.0
98	6.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03275071761632765
2	0.03097150358083299
3	0.027347178693714235
4	0.025963345554996162
5	0.03215764627116276
6	0.0016474204032357973
7	0.001120245874200342
8	0.003953808967765913
9	5.271745290354551E-4
10-14	0.007499057675529349
15-19	0.02564704083757489
20-24	0.021403285878839478
25-29	0.002372285380659548
30-34	0.0024908996496925255
35-39	0.006220659442618371
40-44	0.013456129853629994
45-49	0.008487509917470827
50-54	0.005838457909067665
55-59	0.004480983496801368
60-64	0.010503952491031443
65-69	0.014839962992348061
70-74	0.01895192431882461
75-79	0.00836889564843785
80-84	0.011993220535556603
85-89	0.009752728787155919
90-94	0.011518763459424696
95-99	0.01018764777361017
100-101	0.007149804550043361
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1517524.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.667686527808648
#Duplication Level	Relative count
1	100.0
2	7.344180561750384
3	2.943171195170682
4	2.0193441561218815
5	1.6118093445838195
6	1.2134394838299718
7	0.9843157142246146
8	0.799183708383486
9	0.6794283514697526
10++	4.604471273989259
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	321825	2.9536328	5.5015755	90-94
GCTGC	198305	2.7583735	6.5275044	90-94
CTGTC	296120	2.7177184	5.1365843	90-94
ACACA	403380	2.5310855	8.830467	6
CAAAG	374840	2.4010112	5.027337	4
GACGC	165635	2.331405	6.669856	95-97
CGCTG	166365	2.314096	6.2427216	95-97
CTGCC	169635	2.3114212	6.1395373	95-97
CTTTG	368345	2.277008	9.2214365	9
GCCGA	160345	2.256945	6.313138	90-94
TACAC	363255	2.252464	11.2764635	5
GAGAG	228895	2.2416975	5.609098	7
CGACG	153650	2.1627092	6.3580956	95-97
CACAT	344150	2.1339982	6.2367377	7
TGCCG	148170	2.0610082	6.0898023	95-97
CCGAC	149340	2.0591397	5.9050922	95-97
GCTTT	308455	1.9067845	5.3676305	1
CTGAC	204070	1.8952305	5.0149245	95-97
ATACA	423610	1.7903244	5.3343806	4
GCCAA	187140	1.7587155	5.3428917	1
TGGCT	181520	1.7006583	5.370366	6
CACCA	176845	1.6280432	5.388685	7
GACTC	171200	1.5899614	5.940596	7
TTGAG	248570	1.5873055	6.298907	9
GAGTC	163265	1.5478605	7.9678235	9
TATAC	357370	1.4925796	6.0110197	5
ATGGA	227080	1.4673607	5.0716314	4
CCATG	157180	1.4597555	6.566633	9
GTGTA	228085	1.4564935	12.251184	1
AAGAC	227275	1.4557939	6.027832	5
CATGA	229165	1.450609	6.078126	9
GTCTT	225005	1.3909194	6.7350273	1
TATGA	323910	1.3810189	6.98835	4
GACTT	220235	1.3776609	7.3777165	7
TCCAT	223690	1.3707135	5.1155143	2
TCATA	325220	1.358303	7.34	2
TGAGT	210915	1.3468502	5.722957	8
ACTCA	215320	1.3351517	5.635371	8
ATGAG	201395	1.3013877	5.3367276	7
AACAC	203870	1.2792215	5.1864862	5
GATTG	199355	1.2730309	6.9027843	7
GTGTT	201680	1.2727073	5.9294596	1
GTTCT	197150	1.2187275	5.2836676	1
ACACC	131910	1.2143697	6.0180655	6
ACCAT	192265	1.1921929	5.506065	8
TGGAC	124430	1.1796789	8.075593	5
ACATG	184065	1.1651272	6.418934	8
GTCCA	125090	1.1617306	8.510098	1
ACACT	179440	1.1126679	5.4878597	6
GGACT	115210	1.0922673	7.7699685	6
AGACT	164075	1.0385908	5.8999634	6
TGTAT	245850	1.0358558	5.673576	2
GTATG	160600	1.0255512	6.545086	3
GAGTA	154920	1.0010724	5.225326	1
CATAC	156710	0.97172403	6.289329	3
GTATA	182250	0.77703893	7.338015	1
>>END_MODULE