##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-98_TAAGGCGA-CTCTCTAT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1517524	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47832324233422	34.0	31.0	34.0	31.0	34.0
2	32.65549078630717	34.0	31.0	34.0	31.0	34.0
3	32.76671275050675	34.0	31.0	34.0	31.0	34.0
4	36.27103492267668	37.0	37.0	37.0	35.0	37.0
5	36.004873728520934	37.0	35.0	37.0	35.0	37.0
6	36.09685909415601	37.0	36.0	37.0	35.0	37.0
7	36.09738165590791	37.0	36.0	37.0	35.0	37.0
8	36.121062335752185	37.0	36.0	37.0	35.0	37.0
9	37.9881557062689	39.0	38.0	39.0	35.0	39.0
10-14	38.1713753456288	39.4	38.2	39.4	35.2	39.4
15-19	39.31718193583758	40.8	39.0	41.0	36.0	41.0
20-24	39.248323057823136	40.0	39.0	41.0	36.0	41.0
25-29	39.06476035963847	40.0	39.0	41.0	36.0	41.0
30-34	38.87051631473374	40.0	38.2	41.0	35.2	41.0
35-39	38.64700643943687	40.0	38.0	41.0	34.8	41.0
40-44	38.42002841470711	40.0	38.0	41.0	34.0	41.0
45-49	38.24948903608773	40.0	38.0	41.0	33.6	41.0
50-54	38.19599505510292	40.0	37.6	41.0	33.8	41.0
55-59	37.822256386060445	39.8	36.8	41.0	33.0	41.0
60-64	37.27617632406473	39.0	35.6	40.8	32.4	41.0
65-69	36.555044664861974	37.8	35.0	40.0	31.0	41.0
70-74	35.977993758253575	36.6	35.0	39.2	31.0	41.0
75-79	34.7790209578234	35.2	34.0	37.4	30.4	39.2
80-84	34.565130436157844	35.0	34.0	36.6	31.0	38.2
85-89	33.931832115999484	35.0	34.0	35.6	30.6	36.8
90-94	33.51599394803641	35.0	34.0	35.0	30.0	36.0
95-99	33.24057451480174	35.0	34.0	35.0	29.2	35.6
100-101	32.89622997725242	35.0	33.5	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	5.0
14	12.0
15	66.0
16	108.0
17	229.0
18	409.0
19	742.0
20	1065.0
21	1604.0
22	2185.0
23	3071.0
24	4330.0
25	5572.0
26	7086.0
27	9456.0
28	11903.0
29	15101.0
30	19210.0
31	24093.0
32	31851.0
33	43086.0
34	62471.0
35	99842.0
36	187338.0
37	371289.0
38	507627.0
39	107692.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04659959249409	23.577485430213954	11.24318297437141	24.132732002920545
2	15.344564986004922	23.168167010364993	40.12577447458313	21.361493529046957
3	16.068411438632932	26.828834338040124	32.83651527092817	24.266238952398776
4	11.24904779100693	18.323861764294996	43.97643793442476	26.450652510273315
5	10.410049528047002	39.456970697003804	39.25776462184453	10.875215153104662
6	25.695409100613897	43.88154651919838	19.288261668349232	11.134782711838495
7	24.373123588160713	33.7120203700238	26.1586637180038	15.756192323811682
8	21.09877669150537	39.13104504442763	23.73695572524718	16.033222538819814
9	22.71232613125064	14.956534460081027	25.021680052506586	37.309459356161746
10-14	20.43879371924266	25.975457389800756	32.73673431194498	20.8490145790116
15-19	19.626959364157383	31.97818580941539	28.570347851964627	19.8245069744626
20-24	19.604118523259213	30.075647367656643	30.45069478070329	19.86953932838085
25-29	19.60967305963231	30.72111371184952	29.77894250786318	19.89027072065499
30-34	19.45548872604596	30.338267387922762	29.94230759599079	20.263936290040483
35-39	19.653235894076076	30.69862686962322	29.74234176196269	19.90579547433801
40-44	19.32745994466815	30.443608176856223	30.241187114938796	19.987744763536835
45-49	19.601208387506823	30.257580113032496	30.098211153981058	20.043000345479623
50-54	19.333030312740195	29.778603766026436	30.650981329158032	20.23738459207534
55-59	19.5572164440087	29.779015635720977	30.65756573864239	20.006202181627934
60-64	19.07978314205609	29.434098556592385	30.93824264048309	20.54787566086844
65-69	19.45530047670438	29.614450253910512	30.314825301788613	20.615423967596495
70-74	19.265825787829662	29.409961382837107	30.316568476269556	21.00764435306367
75-79	19.54532766188959	29.825241193196273	29.783330077973126	20.846101066941014
80-84	19.42460322428742	29.744204985002504	29.53570688171581	21.29548490899427
85-89	19.546327472812216	29.763462027384364	29.30513173091484	21.38507876888858
90-94	19.29901813351792	29.2801631550049	29.853904888982846	21.566913822494335
95-99	19.535542154145272	29.203984433721736	29.334002159098205	21.92647125303479
100-101	19.308689681349357	28.312435256378155	30.380145552887466	21.998729509385022
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.17933159541464
2	36.70605851505188
3	40.33465039103171
4	37.699700301280245
5	21.285264681151666
6	36.83019181245239
7	40.1293159119724
8	37.131999230325185
9	60.021785487412394
10-14	41.28780829825426
15-19	39.45146633861999
20-24	39.47365785164006
25-29	39.4999437802873
30-34	39.71942501608645
35-39	39.55903136841408
40-44	39.31520470820498
45-49	39.64420873298645
50-54	39.570414904815536
55-59	39.56341862563663
60-64	39.62765880292453
65-69	40.07072444430088
70-74	40.27347014089334
75-79	40.391428728830604
80-84	40.72008813328169
85-89	40.9314062417008
90-94	40.865931956012254
95-99	41.462013407180066
100-101	41.30741919073438
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	1.0
5	0.0
6	2.0
7	7.0
8	12.0
9	17.0
10	28.5
11	40.5
12	59.0
13	97.5
14	152.5
15	231.0
16	349.5
17	529.5
18	801.0
19	1159.0
20	1661.5
21	2470.5
22	3620.0
23	5234.5
24	7204.0
25	9466.0
26	12390.5
27	16001.0
28	20149.5
29	24524.0
30	29410.5
31	34731.5
32	40068.0
33	46427.5
34	54040.0
35	62843.0
36	73914.0
37	84535.0
38	91483.5
39	92839.5
40	88480.5
41	81567.0
42	73479.0
43	66299.5
44	60809.5
45	55503.5
46	50550.5
47	45392.5
48	39947.5
49	34279.5
50	29291.5
51	25638.5
52	22629.5
53	20040.5
54	17457.0
55	15009.0
56	13010.0
57	11226.0
58	9452.0
59	7940.5
60	6515.0
61	5549.0
62	4528.5
63	3153.0
64	2311.5
65	1953.5
66	1559.0
67	1185.0
68	1001.0
69	958.0
70	918.0
71	787.5
72	675.5
73	555.0
74	432.5
75	339.5
76	220.0
77	132.5
78	79.5
79	55.5
80	38.0
81	22.0
82	16.0
83	11.0
84	7.5
85	4.5
86	5.0
87	4.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013508847306533536
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.001080707784522683
20-24	6.062507083907735E-4
25-29	0.017304503915588815
30-34	0.006405170527780779
35-39	0.043109697111874345
40-44	0.05170264193515227
45-49	0.05219027837451006
50-54	0.05241432754935013
55-59	0.04206852741702932
60-64	0.053218268706129196
65-69	0.04289882730026016
70-74	0.004217396232283641
75-79	0.0017660346722687746
80-84	2.7676662774361394E-4
85-89	0.003914270878088254
90-94	0.0030839709948574127
95-99	0.0025436171025960707
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1517524.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.434795050337748
#Duplication Level	Relative count
1	100.0
2	7.665603706229685
3	3.1255952271233096
4	2.0485029461822517
5	1.5735501035311177
6	1.2208684325327337
7	0.980012657216764
8	0.8239479456599879
9	0.6451493981677757
10++	4.802369234361271
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	322560	2.9143403	5.3797417	90-94
AGAAG	426900	2.8691797	5.0866723	5
CCGAG	202945	2.8390994	7.03855	95-97
CTCCG	208590	2.7488234	7.037125	95-97
GAGCC	195410	2.7336884	6.665482	95-97
CTGTC	298190	2.6941564	5.097612	90-94
CGAGC	184560	2.5819023	6.55017	95-97
TCTCC	295100	2.5391505	5.255306	95-97
ACACA	404360	2.4647837	9.259132	6
GCCCA	183575	2.4457119	6.1511064	90-94
CAAAG	378455	2.4223413	5.03041	4
AGCCC	181285	2.4152029	6.010281	90-94
CTTTG	367995	2.2795436	9.472551	9
ATCTC	374580	2.2339761	5.2648683	95-97
TACAC	365655	2.2046714	11.716539	5
CCCAC	173330	2.1991506	5.8062344	90-94
CACAT	344015	2.0741954	6.653403	7
CCACG	154310	2.0558236	5.8048563	90-94
GCTTT	309105	1.9147499	5.578076	1
ATACA	429310	1.7941468	5.7625647	4
AGGCG	119165	1.750497	5.643438	95-97
GCCAA	187900	1.7351348	5.028665	1
TGGCT	182630	1.7326558	5.763556	6
GGCGA	117900	1.7319149	5.7170377	95-97
TTGGC	180525	1.7126851	5.1103835	5
GAGAG	163880	1.6686041	5.6573267	7
TTGAG	247885	1.6300657	6.4817386	9
GACTC	173845	1.5879259	5.726191	7
AGACT	249855	1.5818701	5.7094593	6
GAGTC	164805	1.580698	8.147266	9
CACCA	179570	1.5791732	5.721999	7
ATGGA	224415	1.4919186	5.195861	4
TATAC	360640	1.4908108	5.974614	5
AAGAC	228830	1.4646508	5.8155394	5
GTCTT	236150	1.4628305	6.7195253	1
CATGA	229960	1.455912	6.1852484	9
CCATG	157400	1.437715	6.6119137	9
TATGA	322655	1.4005463	7.2017555	4
TGAGT	212630	1.3982327	5.923793	8
GACTT	218620	1.3690971	7.308357	7
TCATA	328395	1.3575165	7.7455497	2
ATGAG	202000	1.3429029	5.2732186	7
GTGTT	203175	1.3215598	6.3997808	1
GATTG	198545	1.305611	7.192721	7
ACTCA	216190	1.3034906	5.1186767	8
GTGTA	195735	1.287133	12.544571	1
AACAC	202485	1.234251	5.233937	5
GTATG	187195	1.2309748	6.791001	3
GTTCT	198255	1.22809	5.403856	1
TGGAC	123355	1.1831377	8.658796	5
ACATG	185340	1.1734158	7.0416665	8
ACACC	132945	1.1691439	6.0929475	6
GTCCA	127835	1.1676639	8.030312	1
ACCAT	192835	1.1626745	5.668255	8
ATTGA	261730	1.1360897	5.0088186	8
GGACT	115185	1.1047766	8.054261	6
ACACT	181060	1.0916786	5.639022	6
TGTAT	243030	1.0434716	5.7588987	2
GAGTA	156380	1.0396196	5.6254234	1
TGTAG	150455	0.98937625	5.1434646	2
CATAC	160065	0.96509206	6.7206397	3
GTATA	183085	0.7947159	7.3081155	1
>>END_MODULE