##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-184_AAGAGGCA-CTAAGCCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2577568	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.615074364672434	31.0	31.0	34.0	30.0	34.0
2	31.812092251300452	33.0	31.0	34.0	30.0	34.0
3	31.953217917044284	34.0	31.0	34.0	30.0	34.0
4	35.493540810562514	37.0	35.0	37.0	33.0	37.0
5	35.353298923636544	37.0	35.0	37.0	33.0	37.0
6	35.49344381991086	37.0	35.0	37.0	33.0	37.0
7	35.509624576344834	37.0	35.0	37.0	33.0	37.0
8	35.56280299879577	37.0	35.0	37.0	33.0	37.0
9	37.36398263789743	39.0	37.0	39.0	34.0	39.0
10-14	37.470781527393264	39.2	37.2	39.4	34.0	39.4
15-19	38.498377850749236	40.0	38.0	41.0	34.0	41.0
20-24	38.379973680616764	40.0	38.0	41.0	34.0	41.0
25-29	38.17061889346857	40.0	38.0	41.0	33.0	41.0
30-34	37.8818744646116	40.0	38.0	41.0	32.6	41.0
35-39	37.518370494978214	40.0	37.0	41.0	31.6	41.0
40-44	37.44455618629654	40.0	37.0	41.0	31.4	41.0
45-49	37.25317881041353	40.0	36.4	41.0	31.0	41.0
50-54	36.5337532899229	38.6	35.4	40.2	30.4	40.6
55-59	37.13997628772548	39.6	35.6	41.0	31.0	41.0
60-64	36.6673231511254	39.0	35.0	41.0	30.8	41.0
65-69	35.989332037020944	37.4	35.0	40.0	29.6	41.0
70-74	35.16442476008392	36.2	34.0	39.2	29.0	40.8
75-79	34.223516353399795	35.2	34.0	37.6	28.4	39.4
80-84	33.29934504152751	35.0	33.0	36.2	26.6	38.0
85-89	32.492983308296814	35.0	32.8	35.2	25.4	36.6
90-94	31.845793476641546	35.0	32.0	35.0	24.2	36.0
95-99	31.25697323989125	34.4	31.0	35.0	21.2	35.0
100-101	30.29279305143453	34.0	30.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	7.0
6	87.0
7	259.0
8	634.0
9	821.0
10	1007.0
11	1396.0
12	2069.0
13	2741.0
14	3479.0
15	4120.0
16	4736.0
17	5652.0
18	6463.0
19	7539.0
20	8484.0
21	9792.0
22	11218.0
23	12906.0
24	14994.0
25	17701.0
26	20449.0
27	24422.0
28	29480.0
29	35694.0
30	43846.0
31	55390.0
32	72331.0
33	97430.0
34	138981.0
35	208987.0
36	344423.0
37	593164.0
38	668699.0
39	128095.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.45288796265034	23.224047533113676	10.855813375867864	24.467251128368126
2	15.472414084832812	23.616163701603472	39.47153547093162	21.4398867426321
3	15.984703959367854	27.9091426983681	32.380603490427944	23.7255498518361
4	11.239719260229128	18.325581142657125	43.86710264169512	26.567596955418633
5	11.11653569403593	38.96544751968276	38.56055716762259	11.35745961865872
6	25.82811029960912	43.48566939214945	19.403575135061736	11.282645173179699
7	23.932262912823553	33.44752428077162	26.9899680084297	15.630244797975134
8	21.375876096272144	38.138611863890574	24.09868534637447	16.386826693462815
9	23.309928715363483	14.74762507400427	24.51657656832796	37.42586964230429
10-14	20.89294718399971	25.55659726269162	32.38707233339485	21.163383219913825
15-19	20.261070801471266	31.13991598099576	28.233104928138047	20.36590828939493
20-24	20.18084463389171	29.673422322223225	29.795810760931683	20.34992228295338
25-29	20.15676664393416	30.03398703075205	29.452209079404696	20.357037245909098
30-34	20.05186699936947	29.902057624791873	29.49734232452366	20.548733051314997
35-39	20.176603028068	30.085756332206753	29.464763005161366	20.27287763456388
40-44	19.92057039326091	29.828279668514828	29.861788573091047	20.389361365133215
45-49	19.97689569703891	29.613114279854457	29.940961964345313	20.469028058761317
50-54	19.65337896842733	29.28139004736343	30.38801883239113	20.67721215181811
55-59	19.669336170651263	29.249024309847037	30.491170834391223	20.590468685110473
60-64	19.381235457147547	28.880908952286056	30.65513210157774	21.08272348898866
65-69	19.725723956456296	28.939904994538256	30.093451837172697	21.24091921183275
70-74	19.818161273569544	28.685333046085038	29.94151403237938	21.554991647966038
75-79	20.236973293634993	28.867668468011548	29.488370822573984	21.406987415779476
80-84	20.475985535805773	28.678591033894385	29.305078934899175	21.54034449540067
85-89	20.830605708162885	28.589973762030652	29.15505388980301	21.424366640003452
90-94	21.066304365792274	28.124109777599504	29.469739985024667	21.339845871583556
95-99	21.661072613329146	27.818825150247147	29.04337613929383	21.47672609712988
100-101	22.133644499926767	27.133377757990374	29.369257167355833	21.363720574727026
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.92013909101846
2	36.91230082746491
3	39.710253811203955
4	37.80731621564776
5	22.47399531269465
6	37.11075547278882
7	39.56250771079869
8	37.76270278973495
9	60.73579835766777
10-14	42.056330403913535
15-19	40.62697909086619
20-24	40.53076691684509
25-29	40.51380388984325
30-34	40.60060005068446
35-39	40.449480662631885
40-44	40.30993175839412
45-49	40.44592375580023
50-54	40.330591120245444
55-59	40.259804855761736
60-64	40.4639589461362
65-69	40.966643168289046
70-74	41.373152921535585
75-79	41.643960709414465
80-84	42.01633003120644
85-89	42.25497234816633
90-94	42.406150237375826
95-99	43.13779871045902
100-101	43.49736507465379
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	5.0
2	6.0
3	12.5
4	23.0
5	30.0
6	37.0
7	43.0
8	55.5
9	78.5
10	89.0
11	117.5
12	189.5
13	269.0
14	395.0
15	570.0
16	832.5
17	1263.5
18	1886.0
19	2713.5
20	3953.5
21	5805.5
22	8300.5
23	11554.0
24	15321.5
25	19646.0
26	25134.5
27	31021.5
28	37441.5
29	44498.0
30	51943.0
31	60083.5
32	68076.0
33	77051.0
34	87629.5
35	99010.0
36	111977.5
37	123415.0
38	130059.0
39	131271.5
40	127927.5
41	122266.0
42	114651.5
43	106815.5
44	99776.0
45	92245.0
46	84687.5
47	77013.0
48	68435.0
49	59600.0
50	51941.5
51	46187.0
52	41657.0
53	37959.5
54	34112.5
55	30348.5
56	27368.5
57	24600.5
58	21903.0
59	19308.0
60	16899.5
61	15147.5
62	13538.0
63	11477.0
64	9803.0
65	8915.5
66	8104.0
67	7259.0
68	6590.5
69	6166.5
70	5697.5
71	5099.0
72	4483.5
73	3810.0
74	3146.0
75	2502.0
76	1999.0
77	1623.5
78	1252.5
79	909.5
80	664.0
81	460.5
82	332.5
83	243.5
84	175.0
85	144.0
86	102.0
87	80.0
88	67.5
89	48.5
90	37.0
91	32.0
92	26.0
93	26.5
94	25.0
95	16.5
96	14.0
97	16.0
98	12.0
99	4.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03251126643409602
2	0.03033867583706812
3	0.026847012377559
4	0.025683124557722627
5	0.031851730002855405
6	0.0016682392084321344
7	5.43147649256974E-4
8	0.0032200896348806318
9	5.43147649256974E-4
10-14	0.006781586363579933
15-19	0.024333014686712437
20-24	0.020453388620591192
25-29	0.0021493128406311684
30-34	0.0020329240586475315
35-39	0.005780642838520652
40-44	0.01329159890253138
45-49	0.008248085016573762
50-54	0.005291809954189376
55-59	0.004283107176997852
60-64	0.010513786639188569
65-69	0.014308060931855144
70-74	0.018280798023563298
75-79	0.007658381854523334
80-84	0.010847434480874996
85-89	0.009093843498988194
90-94	0.010187898049634384
95-99	0.00961371339184844
100-101	0.006614762442736719
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2577568.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	32.698335412799366
#Duplication Level	Relative count
1	100.0
2	7.738418210474641
3	2.955843216288143
4	2.1374312068831873
5	1.7763480867116244
6	1.4714621605560292
7	1.286076014779216
8	1.138154665013048
9	0.955998243030256
10++	9.729219957109269
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	496175	3.6943593	8.34513	90-94
GTCTC	696885	3.5472434	5.896762	80-84
CTGTC	667985	3.4001384	5.588844	65-69
GACGC	430165	3.2816327	8.107093	85-89
CGCTG	440380	3.2789278	7.9999146	90-94
TCTCT	931535	3.241565	4.8387957	70-74
GCCGA	415105	3.1667435	8.287659	90-94
CTGCC	434760	3.1476967	7.812719	90-94
CTCTT	880550	3.064147	4.6862226	70-74
CGACG	394915	3.0127182	8.385633	95-97
TGCCG	397385	2.958801	7.9110827	90-94
CCGAC	381905	2.8330173	7.825469	95-97
ACACA	750410	2.8087044	6.7774925	6
GAAGG	485035	2.7411084	6.668976	85-89
CTGAC	486465	2.537068	5.776575	95-97
TACAC	688670	2.5157528	8.100142	5
TGACG	464035	2.4888127	5.8894553	95-97
GACGA	423835	2.3291044	6.2803826	95-97
GGCTT	429390	2.2477229	5.8801255	95-97
CGAAG	404690	2.2238967	6.0351734	95-97
ACGCT	424395	2.2133534	5.477558	85-89
ATACA	808045	2.1263244	5.136262	6
AAGGC	380580	2.0914047	5.711941	90-94
AGGCT	380420	2.040351	5.6997056	90-94
TATAC	721635	1.8533651	5.9664063	5
CTTTG	513070	1.8360865	7.7940598	9
GGTGG	219845	1.7311821	5.326646	95-97
AGGTG	284255	1.5678719	5.263052	95-97
GAGAG	267940	1.514226	5.17158	7
GTGTA	351570	1.3256836	8.917418	1
AAGAC	321670	1.2381662	5.388767	5
GAGTC	219515	1.1773503	5.971968	9
TATGA	443535	1.1714729	5.8142467	4
TCATA	452195	1.1613661	5.788272	2
GATTG	301835	1.1381453	5.443838	7
GTCTT	317585	1.1365185	5.8052273	1
GACTT	301885	1.1069008	5.503336	7
GTGTT	293500	1.0801537	5.2541704	1
ACATG	275425	1.034716	5.084922	8
TGGAC	192635	1.0331817	6.6032495	5
GTCCA	192110	1.0019141	6.9852734	1
GGACT	172805	0.92682517	5.97954	6
TATAA	431370	0.79805076	5.0570917	2
GTATA	263795	0.6967403	7.0841446	1
>>END_MODULE