##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-184_AAGAGGCA-CTAAGCCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2577568	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.440952867198845	34.0	31.0	34.0	31.0	34.0
2	32.62995893803772	34.0	31.0	34.0	31.0	34.0
3	32.73934965052329	34.0	31.0	34.0	31.0	34.0
4	36.254902683459754	37.0	37.0	37.0	35.0	37.0
5	35.97187271102062	37.0	35.0	37.0	35.0	37.0
6	36.0615083675775	37.0	36.0	37.0	35.0	37.0
7	36.061950644949036	37.0	36.0	37.0	35.0	37.0
8	36.089008321021986	37.0	36.0	37.0	35.0	37.0
9	37.95827345777105	39.0	38.0	39.0	35.0	39.0
10-14	38.137574488820476	39.2	38.2	39.4	35.2	39.4
15-19	39.2618537318899	40.0	39.0	41.0	36.0	41.0
20-24	39.183436014103215	40.0	39.0	41.0	36.0	41.0
25-29	38.97411179840842	40.0	38.6	41.0	35.6	41.0
30-34	38.75631626401321	40.0	38.0	41.0	35.0	41.0
35-39	38.49172902518963	40.0	38.0	41.0	34.0	41.0
40-44	38.210856357620834	40.0	38.0	41.0	33.6	41.0
45-49	38.00015704726316	40.0	37.2	41.0	33.0	41.0
50-54	37.923122028206436	40.0	37.0	41.0	33.0	41.0
55-59	37.51589141392196	39.4	36.0	41.0	32.8	41.0
60-64	36.94719262498604	38.8	35.0	40.4	31.8	41.0
65-69	36.2111885312046	37.4	35.0	40.0	31.0	41.0
70-74	35.6513999242697	36.4	34.8	39.0	31.0	40.8
75-79	34.48734039218364	35.2	33.4	37.4	29.4	39.2
80-84	34.31813965722728	35.0	34.0	36.4	30.6	38.0
85-89	33.7127932997306	35.0	34.0	35.4	30.0	36.6
90-94	33.305359548225304	35.0	34.0	35.0	29.0	36.0
95-99	33.02533620839489	35.0	33.4	35.0	29.0	35.4
100-101	32.66410876454084	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	0.0
12	4.0
13	16.0
14	43.0
15	132.0
16	302.0
17	560.0
18	916.0
19	1453.0
20	2390.0
21	3315.0
22	4555.0
23	6165.0
24	8402.0
25	10849.0
26	13998.0
27	17871.0
28	22729.0
29	28527.0
30	36505.0
31	45924.0
32	59996.0
33	80735.0
34	118154.0
35	189620.0
36	339960.0
37	626512.0
38	792332.0
39	165444.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.92489509491117	23.293740456119878	11.248743001154576	24.532621447814375
2	15.66435129319209	23.433514429632023	39.336917493188515	21.565216783987374
3	16.104870948118535	27.662121814051076	32.60953736235086	23.623469875479522
4	11.424722839513835	18.3545109188196	43.6589839724888	26.56178226917777
5	10.992842865833218	39.053208295571636	38.714051384871325	11.23989745372382
6	25.877377434853322	43.65169027548449	19.281741548622577	11.189190741039615
7	23.843483469689257	33.433996697662295	27.044912103191848	15.677607729456605
8	21.37064861140424	38.1560447677811	24.06916131795553	16.40414530285913
9	23.18751629442948	14.610865746315907	24.46018882915989	37.74142913009472
10-14	20.845238612521573	25.51584284100361	32.44159610920061	21.197322437274206
15-19	20.206758253678622	31.157347761137977	28.259384833982892	20.37650915120051
20-24	20.150303667599438	29.668509136099196	29.827326297415475	20.35386089888589
25-29	20.123093122525276	30.0646795331414	29.448406813565825	20.363820530767498
30-34	20.01813465270627	29.861557407388727	29.5337368946677	20.586571045237307
35-39	20.14500958416753	30.068560584801507	29.477666945113263	20.308762885917705
40-44	19.905747538515392	29.796860512986846	29.860434034262006	20.436957914235755
45-49	19.954420009919968	29.565321757295248	29.986388575315555	20.493869657469233
50-54	19.60962551401225	29.258639225353033	30.391577807216446	20.74015745341827
55-59	19.572121014716743	29.211468977593658	30.452276738050728	20.764133269638872
60-64	19.19746082141347	28.84911407609462	30.55998067233206	21.393444430159857
65-69	19.44878771970584	29.023934023373965	29.853252711035633	21.67402554588456
70-74	19.350790977566827	28.95488007459101	29.598899509472236	22.095429438369923
75-79	19.701341634830598	29.43261506087036	28.89097960952494	21.9750636947741
80-84	19.692951807032596	29.56355515372894	28.422329807056496	22.321163232181966
85-89	19.831845240635555	29.523518061885806	28.204106735455227	22.44052996202341
90-94	19.80355828874939	29.11239406099886	28.47718100510984	22.606866645141913
95-99	19.925425666829923	28.85114495050415	28.30462499293892	22.91880438972701
100-101	19.85163522485942	28.111578908163747	29.018384010341542	23.01840185663529
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.45751654272554
2	37.22956807717946
3	39.72834082359806
4	37.9865051086916
5	22.23274031955704
6	37.06656817589293
7	39.521091199145864
8	37.77479391426336
9	60.92894542452421
10-14	42.04256104979578
15-19	40.58326740487913
20-24	40.50416456648533
25-29	40.486913653292774
30-34	40.60470569794358
35-39	40.453772470085234
40-44	40.34270545275115
45-49	40.4482896673892
50-54	40.34978296743052
55-59	40.33625428435562
60-64	40.590905251573325
65-69	41.1228132655904
70-74	41.44622041593675
75-79	41.67640532960469
80-84	42.01411503921456
85-89	42.27237520265897
90-94	42.4104249338913
95-99	42.84423005655694
100-101	42.87003708149471
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	1.0
5	2.0
6	4.0
7	8.0
8	13.0
9	25.0
10	37.5
11	62.0
12	116.5
13	206.0
14	313.0
15	477.5
16	744.5
17	1183.0
18	1870.5
19	2697.0
20	3892.5
21	5946.0
22	8533.0
23	11653.0
24	15472.0
25	19817.0
26	25029.5
27	30792.5
28	37182.5
29	44042.0
30	51552.0
31	59863.5
32	67907.0
33	76570.0
34	86997.0
35	98122.5
36	110942.0
37	122891.0
38	130054.0
39	131831.0
40	128831.5
41	122928.5
42	114829.0
43	107090.0
44	100465.5
45	93379.0
46	86017.5
47	78395.5
48	69827.5
49	60503.0
50	52362.0
51	46329.5
52	41368.5
53	37475.5
54	33842.5
55	30009.5
56	27061.0
57	24370.5
58	21582.0
59	18982.5
60	16601.5
61	14961.0
62	13445.0
63	11454.0
64	9766.5
65	8787.0
66	8006.0
67	7294.0
68	6665.0
69	6222.5
70	5825.5
71	5056.0
72	4339.0
73	3819.5
74	3187.0
75	2548.5
76	1990.0
77	1529.0
78	1109.5
79	790.5
80	577.5
81	394.5
82	244.5
83	163.0
84	101.0
85	61.0
86	42.5
87	27.5
88	18.5
89	13.5
90	10.0
91	5.5
92	3.5
93	3.5
94	2.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011716470719686154
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.001024221281456008
20-24	4.965921364635191E-4
25-29	0.017931631677612386
30-34	0.007060919440340662
35-39	0.04389408931209574
40-44	0.05191715601683448
45-49	0.052367192640504545
50-54	0.05294913655042272
55-59	0.042722442240127126
60-64	0.05294913655042272
65-69	0.04048001837390905
70-74	0.003848589057592273
75-79	0.0015906466871097097
80-84	2.0174055543830465E-4
85-89	0.003305441408335299
90-94	0.0027390160026815975
95-99	0.002296738631143776
100-101	7.759252132242486E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2577568.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	33.41215908943258
#Duplication Level	Relative count
1	100.0
2	8.056899781454886
3	3.0648350504735142
4	2.156181704651889
5	1.7724268914559267
6	1.5096524091997086
7	1.2904568633572693
8	1.1298001873243835
9	1.0120720158185035
10++	10.09405245082735
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	485545	3.6776097	8.394883	85-89
GAGCC	469540	3.556384	8.252384	85-89
GTCTC	697780	3.5488865	5.9001255	80-84
CGAGC	465500	3.5257847	8.272951	85-89
CTCCG	495415	3.4918094	7.770243	95-97
CTGTC	670785	3.4115908	5.5972095	65-69
TCTCT	935955	3.2221553	4.800406	70-74
AGCCC	444590	3.1587949	7.6507683	90-94
GCCCA	441615	3.1376572	7.76032	90-94
CTCTT	882395	3.0377676	4.616122	70-74
TCTCC	629905	3.005206	5.789335	95-97
ATCTC	811010	2.8144796	7.16536	95-97
CCCAC	421815	2.8113143	7.2731	90-94
CCACG	391930	2.7846475	7.550173	90-94
TCCGA	521535	2.6738527	5.865024	95-97
ACACA	753460	2.6570077	6.3483562	6
GAGGC	313985	2.5352416	8.517837	95-97
TACAC	692705	2.423263	7.8598084	5
CGAGA	436095	2.4026463	6.280408	95-97
AGAGG	406835	2.389469	6.6645365	90-94
GAGAC	429645	2.36711	6.3026686	95-97
ACGAG	415495	2.2891512	6.09252	95-97
AAGAG	569820	2.2835948	5.0864935	90-94
CACGA	402630	2.0808487	5.5405083	95-97
CTTTG	513085	1.8830211	8.044593	9
TATAC	723370	1.8260179	6.1978626	5
AGGCA	315655	1.7390872	5.7514744	95-97
GGCAA	315050	1.735754	5.8984003	95-97
GAGAG	265700	1.560539	5.4931192	7
GTCTT	345615	1.2684065	5.7515707	1
CCATG	241465	1.2379645	5.0783906	9
GAGTC	225475	1.2323292	5.919912	9
TATGA	449350	1.2092154	5.7421427	4
AAGAC	320770	1.2058707	5.415186	5
GATTG	300710	1.1859558	5.474516	7
GTGTT	294200	1.1510202	5.50158	1
TCATA	452030	1.1410687	5.672177	2
TGCCG	150975	1.1343857	6.0501294	95-97
GACTT	305300	1.1294657	5.599933	7
GCCGT	148190	1.1134598	5.717384	95-97
GTTCT	303335	1.1132389	5.0397425	1
TGGAC	192620	1.0527608	7.183922	5
GTGTA	253490	0.999727	9.274949	1
GTCCA	193905	0.9941296	6.8017287	1
GGACT	172100	0.9406091	6.2697	6
GTATA	263830	0.70997506	6.9706297	1
ATACT	280785	0.7087913	5.140407	6
>>END_MODULE