##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-183_AAGAGGCA-AAGGAGTA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2532424	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.712884572251724	33.0	31.0	34.0	30.0	34.0
2	31.913419316828463	33.0	31.0	34.0	30.0	34.0
3	32.05615252422185	34.0	31.0	34.0	30.0	34.0
4	35.594030067634804	37.0	35.0	37.0	33.0	37.0
5	35.45775509946202	37.0	35.0	37.0	33.0	37.0
6	35.59777707050636	37.0	35.0	37.0	33.0	37.0
7	35.616240013520645	37.0	35.0	37.0	33.0	37.0
8	35.66585887671259	37.0	35.0	37.0	33.0	37.0
9	37.48395568830496	39.0	37.0	39.0	35.0	39.0
10-14	37.61085410657931	39.2	37.2	39.4	34.4	39.4
15-19	38.662799989259305	40.0	38.0	41.0	34.4	41.0
20-24	38.56319692121066	40.0	38.0	41.0	34.0	41.0
25-29	38.37470621033445	40.0	38.0	41.0	34.0	41.0
30-34	38.1098765451599	40.0	38.0	41.0	33.0	41.0
35-39	37.79109714644942	40.0	37.8	41.0	32.6	41.0
40-44	37.74079735462941	40.0	37.8	41.0	32.8	41.0
45-49	37.57828049331391	40.0	37.0	41.0	32.0	41.0
50-54	36.87534275460982	39.2	36.2	40.2	31.2	40.6
55-59	37.509880730872865	40.0	36.4	41.0	32.0	41.0
60-64	37.070670472243194	39.0	35.4	41.0	31.4	41.0
65-69	36.409952440823496	38.2	35.0	40.2	31.0	41.0
70-74	35.56387974525593	36.6	34.8	39.4	29.8	41.0
75-79	34.57577727900225	35.4	34.0	38.0	29.0	39.6
80-84	33.61339238610912	35.0	33.6	36.4	28.2	38.2
85-89	32.77622459746077	35.0	33.0	35.4	26.2	36.6
90-94	32.10741013353215	35.0	32.4	35.0	25.0	36.0
95-99	31.4800473380445	34.8	31.6	35.0	22.8	35.2
100-101	30.476914805735532	34.0	30.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	6.0
6	60.0
7	272.0
8	513.0
9	773.0
10	824.0
11	1133.0
12	1597.0
13	2089.0
14	2626.0
15	3323.0
16	3771.0
17	4343.0
18	5235.0
19	6043.0
20	7174.0
21	8219.0
22	9457.0
23	11153.0
24	12935.0
25	14950.0
26	17866.0
27	21587.0
28	26131.0
29	31688.0
30	39247.0
31	49995.0
32	65076.0
33	87275.0
34	125122.0
35	193323.0
36	329955.0
37	592227.0
38	709444.0
39	146890.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.1739471858859	23.602581138538692	10.908454587540824	24.315017088034587
2	15.088807413955596	24.12434854944862	39.742532364166095	21.04431167242969
3	15.509870844226658	28.36564762146643	33.05059071811621	23.073890816190705
4	11.211611806453039	18.706729842544814	44.44542311933795	25.636235231664195
5	10.965075304732451	39.33482827582203	38.56402571132547	11.13607070812005
6	25.034808959427323	44.01534674304236	19.743886614800065	11.205957682730254
7	23.278926489554188	34.218895736143	27.243344461108464	15.25883331319435
8	20.992749396704472	38.21080968163451	24.752156206450866	16.044284715210154
9	23.020437013680617	15.167853610091553	25.232159815827977	36.579549560399855
10-14	20.373935056577412	26.030310152974007	32.999353301683975	20.596401488764606
15-19	19.75190732657662	31.581590003110875	28.850600427306876	19.815902243005628
20-24	19.673466382809107	30.176253994956003	30.315036232756988	19.83524338947791
25-29	19.652139794854914	30.4777304580967	29.993433828247145	19.87669591880124
30-34	19.542259055094355	30.390618200554453	30.029084153235402	20.03803859111579
35-39	19.6471934431191	30.567482896422238	29.980453956620543	19.80486970383811
40-44	19.41596823411127	30.167792054783853	30.43388216129177	19.982357549813106
45-49	19.390374569764397	29.8193614670969	30.680069624359234	20.11019433877947
50-54	18.980493541878417	29.561878889695446	31.075625405807006	20.382002162619134
55-59	18.943040130106613	29.573815854153214	31.085044752581346	20.398099263158826
60-64	18.75419355577592	29.08721766682108	31.193831035096924	20.964757742306073
65-69	19.150568513249645	28.98524624568622	30.56347861500629	21.300706626057845
70-74	19.133279345728365	28.69812987610445	30.40693078254012	21.761659995627067
75-79	19.20457247889428	28.75429631500036	30.117072387077908	21.924058819027447
80-84	19.157653542989905	28.342252662865143	30.247312452189668	22.252781341955284
85-89	19.37067322539549	28.08214839439886	30.383176986949678	22.164001393255976
90-94	19.615149578618905	27.572945833804276	30.653114040294398	22.158790547282425
95-99	20.233671987242623	27.061680400539927	30.34946935093923	22.35517826127822
100-101	20.686061552392513	26.39932503128128	30.638157807702232	22.276455608623976
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.48896427392049
2	36.133119086385285
3	38.583761660417366
4	36.84784703811724
5	22.101146012852503
6	36.24076664215758
7	38.53775980274854
8	37.03703411191463
9	59.599986574080475
10-14	40.970336545342015
15-19	39.567809569582245
20-24	39.50870977228701
25-29	39.52883571365615
30-34	39.58029764621014
35-39	39.45206314695721
40-44	39.39832578392438
45-49	39.50056890854386
50-54	39.36249570449755
55-59	39.341139393265436
60-64	39.718951298082
65-69	40.451275139307484
70-74	40.89493934135543
75-79	41.12863129792173
80-84	41.41043488494519
85-89	41.534674618651465
90-94	41.77394012590133
95-99	42.58885024852084
100-101	42.96251716101649
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	4.5
2	11.5
3	21.5
4	24.0
5	26.0
6	35.0
7	40.5
8	53.5
9	80.5
10	104.5
11	130.5
12	192.5
13	276.0
14	388.5
15	591.0
16	845.5
17	1196.0
18	1729.0
19	2482.0
20	3660.0
21	5437.0
22	7880.0
23	10941.0
24	14676.0
25	19359.5
26	24812.0
27	30628.5
28	37285.5
29	44626.5
30	52120.0
31	60111.5
32	68566.0
33	77348.5
34	87178.0
35	98185.0
36	110413.0
37	121855.5
38	129607.5
39	132871.0
40	131569.5
41	127367.0
42	121437.0
43	114931.5
44	108319.5
45	100536.0
46	92303.0
47	83495.5
48	73705.0
49	63535.5
50	54392.0
51	47842.0
52	41840.5
53	35873.0
54	30780.5
55	26010.0
56	22186.0
57	18910.0
58	15772.0
59	13035.5
60	10743.0
61	8975.0
62	7332.5
63	5547.5
64	4359.0
65	3829.5
66	3194.0
67	2616.5
68	2254.0
69	2196.5
70	2128.0
71	1861.5
72	1632.0
73	1346.5
74	1123.0
75	929.5
76	676.5
77	502.0
78	394.0
79	293.5
80	199.5
81	131.5
82	104.0
83	84.0
84	65.5
85	55.0
86	38.5
87	30.0
88	27.0
89	23.0
90	17.0
91	12.0
92	10.0
93	13.5
94	12.0
95	6.5
96	7.5
97	8.5
98	4.0
99	0.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03269594665032396
2	0.030247699437376995
3	0.026654304334503227
4	0.0259040350273098
5	0.030682065878383714
6	0.0011846357482001434
7	8.687328820134385E-4
8	0.0032774922366870635
9	3.5539072446004303E-4
10-14	0.0069814533427261785
15-19	0.026077781603712494
20-24	0.021473497329041264
25-29	0.001990188056976241
30-34	0.0019112123404295648
35-39	0.005796817594526035
40-44	0.013797057680704338
45-49	0.008166089090926322
50-54	0.004904391997548594
55-59	0.003980376113952482
60-64	0.0104642824424346
65-69	0.014349887696531071
70-74	0.01914371369091432
75-79	0.007731722649919603
80-84	0.011175063891354685
85-89	0.008924255969774414
90-94	0.009895657283298531
95-99	0.009556061702147824
100-101	0.006258825536324091
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2532424.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.538046868241686
#Duplication Level	Relative count
1	100.0
2	7.960451493769337
3	2.5460726645714358
4	1.6588605040783633
5	1.325009477455884
6	1.02356523546892
7	0.8560282733909725
8	0.7190637496484169
9	0.6304036784757805
10++	5.5702982647084
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	548665	4.4066644	10.454717	90-94
GACGC	513935	4.307813	10.650259	75-79
GTCTC	830525	4.289414	7.1062574	60-64
CGCTG	511865	4.111101	10.366297	75-79
CTGTC	789490	4.077481	6.948232	55-59
GCCGA	476135	3.990973	10.94504	80-84
CGACG	467050	3.9148226	10.95643	95-97
CTGCC	499290	3.7590435	9.71766	90-94
TGCCG	462405	3.713858	10.24437	80-84
TCTCT	1093350	3.6311738	5.54254	60-64
CCGAC	455270	3.5771694	10.03264	80-84
CTCTT	1016960	3.377471	5.2828884	60-64
TGTCT	935465	3.3143134	5.1798635	55-59
ACACA	866650	3.2716696	6.4769416	6
TGACG	557930	3.2081094	7.437915	85-89
CTGAC	581370	3.1336021	6.88864	95-97
GACGA	504130	3.0252268	8.080811	85-89
TACAC	802130	2.9015129	7.337584	5
ATCTG	782670	2.8939476	5.6721406	70-74
CACAT	791550	2.8632424	5.132355	95-97
ACGCT	517260	2.7880473	6.9788013	75-79
CATCT	801120	2.7767153	5.155639	70-74
ACATC	763050	2.7601504	5.149686	70-74
TCTGA	739005	2.7324946	5.2711377	70-74
AGAAG	596185	2.5613158	5.3868136	5
CCTTG	456225	2.3562663	6.7233734	90-94
CTCCT	484000	2.3432164	6.518458	90-94
ACTCC	449610	2.2716897	6.478904	90-94
TATAC	837150	2.0773215	5.4897156	5
CAAAG	508215	2.0466871	5.1999764	4
GGTGG	210205	1.921331	7.6029973	95-97
TTGTG	506995	1.9162313	5.3125644	95-97
AGAGA	442555	1.9012942	5.4169593	8
GATAC	483870	1.867182	5.1237254	85-89
ACGAT	478295	1.8456689	5.1428146	85-89
TGTGT	484765	1.832211	5.0546966	95-97
GAGAG	281160	1.7998949	6.267929	7
CTTTG	504835	1.7886093	7.5838833	9
AAGAG	379130	1.6288092	5.0804844	5
GTGTA	405405	1.5991132	8.889223	1
CTCGG	194565	1.5626706	7.3027825	95-97
CGGTG	155090	1.3288157	6.9330673	95-97
AAGAC	320900	1.2923305	5.825066	5
GAGTC	215550	1.2394171	6.3492985	9
TATGA	430625	1.1399292	5.1809754	4
GTGTT	300000	1.1338756	5.6777973	1
GTCTT	319370	1.1315145	5.8808556	1
GATTG	286565	1.1303511	5.059528	7
GACTT	297290	1.0992393	5.4384923	7
TCATA	430430	1.068078	5.049161	2
TGGAC	174515	1.0034649	6.152679	5
GTCCA	174405	0.94004834	6.5597363	1
AGACT	240560	0.92828506	5.211582	6
GGACT	161180	0.9267884	5.844497	6
GAGTA	217060	0.89354396	5.177655	1
GTATA	262690	0.69538	7.1841145	1
>>END_MODULE