##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-183_AAGAGGCA-AAGGAGTA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2532424	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46581101742836	34.0	31.0	34.0	31.0	34.0
2	32.64871877695046	34.0	31.0	34.0	31.0	34.0
3	32.75876433014377	34.0	31.0	34.0	31.0	34.0
4	36.2722474593512	37.0	37.0	37.0	35.0	37.0
5	35.99680306299419	37.0	35.0	37.0	35.0	37.0
6	36.08372926492562	37.0	36.0	37.0	35.0	37.0
7	36.0814381793886	37.0	36.0	37.0	35.0	37.0
8	36.10216733058919	37.0	36.0	37.0	35.0	37.0
9	37.97416230457459	39.0	38.0	39.0	35.0	39.0
10-14	38.165806831715386	39.4	38.2	39.4	35.2	39.4
15-19	39.30278539454689	40.8	39.0	41.0	36.0	41.0
20-24	39.23665555215082	40.0	39.0	41.0	36.0	41.0
25-29	39.04332418268031	40.0	39.0	41.0	36.0	41.0
30-34	38.844319987490245	40.0	38.0	41.0	35.0	41.0
35-39	38.61496913628998	40.0	38.0	41.0	34.6	41.0
40-44	38.36823572987778	40.0	38.0	41.0	34.0	41.0
45-49	38.18703637305602	40.0	38.0	41.0	33.4	41.0
50-54	38.13613399651875	40.0	37.4	41.0	33.4	41.0
55-59	37.76395690453099	39.8	36.6	41.0	33.0	41.0
60-64	37.21706262458419	39.0	35.4	40.8	32.0	41.0
65-69	36.493707688759855	37.6	35.0	40.0	31.0	41.0
70-74	35.90237756394663	36.6	35.0	39.2	31.0	41.0
75-79	34.69088241147612	35.2	34.0	37.4	30.4	39.2
80-84	34.45995386238639	35.0	34.0	36.4	31.0	38.2
85-89	33.82489298790408	35.0	34.0	35.6	30.0	36.8
90-94	33.40188886221265	35.0	34.0	35.0	29.6	36.0
95-99	33.11656444576422	35.0	33.8	35.0	29.0	35.4
100-101	32.76408788575689	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	3.0
13	6.0
14	46.0
15	90.0
16	203.0
17	438.0
18	812.0
19	1397.0
20	1978.0
21	2934.0
22	4071.0
23	5643.0
24	7654.0
25	10071.0
26	12694.0
27	16472.0
28	21100.0
29	26706.0
30	33187.0
31	42352.0
32	55001.0
33	74114.0
34	106679.0
35	171083.0
36	312546.0
37	611353.0
38	826211.0
39	187387.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.735398179767685	23.64220999327127	11.227385303566859	24.395006523394187
2	15.179592251007762	24.06933857589184	39.61305589793756	21.13801327516284
3	15.607497006820342	28.06086184619953	33.283447005714685	23.048194141265444
4	11.441527958983173	18.672110199555842	44.22640126613869	25.659960575322298
5	10.790333688197554	39.408645629641796	38.76483558835329	11.036185093807356
6	25.0404355668719	44.13273606631433	19.68434195853459	11.142486408279183
7	23.224941794896907	34.26404899021649	27.292112221334186	15.218896993552422
8	20.990955700941075	38.26203668895888	24.714739711833406	16.032267898266642
9	22.972890795538188	15.020509993587172	25.23337324239543	36.77322596847921
10-14	20.31756925380584	25.97733238983677	33.05333546041263	20.651762895944756
15-19	19.692727701497883	31.586825898037862	28.869331323328694	19.85111507713556
20-24	19.64787043861165	30.13792243969457	30.35218484472175	19.862022276972027
25-29	19.631290904430504	30.521582215264086	29.975840936441166	19.87128594386424
30-34	19.531743508113646	30.353418054719018	30.06031940249893	20.054519034668413
35-39	19.6455003153296	30.552673001070275	29.97762905956245	19.824197624037676
40-44	19.41805293690536	30.14473333727892	30.452939718371823	19.98427400744389
45-49	19.376550421929693	29.798862559031992	30.703984204087636	20.12060281495068
50-54	18.936542788196792	29.563015623082595	31.083152297893058	20.417289290827558
55-59	18.820181352541752	29.45428633146433	31.0831818849091	20.642350431084814
60-64	18.53122316101836	29.07175970794289	31.01784836479609	21.37916876624266
65-69	18.778004855353466	29.14096966593155	30.19756644507839	21.8834590336366
70-74	18.78307080976842	29.214501703844924	29.65949085494892	22.342936631437734
75-79	19.22538330855959	29.727749970442886	28.807005442378557	22.239861278618967
80-84	19.28897384587415	29.91931666278895	28.273301398647018	22.51840809268988
85-89	19.40308833580562	29.79582688146447	28.134107678696697	22.66697710403322
90-94	19.44999855865504	29.286099866645976	28.386724304669524	22.877177270029463
95-99	19.58645340431618	28.87594202358299	28.30312629177533	23.234478280325494
100-101	19.501809333666085	28.125246799114205	28.984601314787728	23.38834255243198
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.13040470316187
2	36.317605526170595
3	38.655691148085786
4	37.101488534305474
5	21.826518782004907
6	36.182921975151075
7	38.44383878844933
8	37.02322359920772
9	59.7461167640174
10-14	40.96933214975059
15-19	39.54384277863344
20-24	39.509892715583675
25-29	39.502576848294744
30-34	39.58626254278206
35-39	39.46969793936728
40-44	39.402326944349255
45-49	39.49715323688037
50-54	39.35383207902435
55-59	39.46253178362657
60-64	39.91039192726102
65-69	40.66146388899006
70-74	41.126007441206156
75-79	41.46524458717856
80-84	41.80738193856403
85-89	42.070065439838835
90-94	42.3271758286845
95-99	42.82093168464168
100-101	42.89015188609806
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	2.0
6	3.5
7	7.0
8	12.5
9	21.5
10	36.5
11	73.0
12	121.5
13	196.0
14	318.0
15	493.0
16	764.5
17	1136.5
18	1657.5
19	2504.0
20	3699.0
21	5453.0
22	7933.5
23	11089.0
24	14841.0
25	19410.0
26	24675.0
27	30384.0
28	36987.5
29	44106.0
30	51539.0
31	59694.5
32	67624.0
33	75895.5
34	85656.5
35	96229.0
36	107987.5
37	119709.0
38	127720.5
39	130605.0
40	129242.5
41	125129.0
42	119136.5
43	112862.5
44	107286.5
45	101135.5
46	94309.0
47	86738.0
48	77555.0
49	67060.0
50	57758.0
51	50674.0
52	44082.0
53	37565.5
54	31788.0
55	26977.5
56	23253.5
57	19712.0
58	16077.5
59	13089.5
60	10807.5
61	9085.0
62	7433.0
63	5595.5
64	4376.5
65	3805.5
66	3164.5
67	2603.0
68	2231.5
69	2180.0
70	2174.0
71	1855.5
72	1609.0
73	1370.0
74	1085.0
75	870.0
76	664.0
77	476.5
78	316.5
79	226.5
80	149.0
81	105.5
82	79.0
83	53.5
84	34.0
85	18.5
86	12.5
87	9.5
88	7.0
89	4.0
90	1.0
91	1.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012912529655381564
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	9.950940284881205E-4
20-24	6.002154457547394E-4
25-29	0.017966975514368842
30-34	0.006744526193086149
35-39	0.04361039067707461
40-44	0.05243197821533835
45-49	0.05286634465634507
50-54	0.05274788108152505
55-59	0.04301017523131987
60-64	0.05360081882022916
65-69	0.040775162453048935
70-74	0.0036565756761111094
75-79	0.0015242313293508513
80-84	2.0533686302135818E-4
85-89	0.0032695946650323957
90-94	0.0026456865043136537
95-99	0.0022271152066162697
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2532424.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.010525646036527
#Duplication Level	Relative count
1	100.0
2	8.186017370793971
3	2.5509514625127454
4	1.7112811881917913
5	1.305880763135588
6	1.039913514575988
7	0.8808245598948415
8	0.7229640913502291
9	0.652940381567794
10++	5.797840321371973
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	574170	4.680569	10.904313	75-79
GAGCC	549765	4.4816227	10.764395	75-79
CTCCG	591300	4.406412	9.577985	75-79
CGAGC	539590	4.3986773	10.709849	75-79
GTCTC	830980	4.381131	7.2405815	60-64
CTGTC	791035	4.1705313	7.06598	55-59
AGCCC	520955	3.915521	9.627443	80-84
GCCCA	514820	3.8694096	9.724379	80-84
TCTCT	1091410	3.7534714	5.706927	60-64
TCTCC	741275	3.6033473	7.032811	70-74
CCACG	466750	3.5081134	9.692309	80-84
CTCTT	1018910	3.5041363	5.4839115	60-64
TGTCT	937495	3.4968982	5.4518394	55-59
CCCAC	496235	3.438807	9.00889	80-84
ATCTC	969870	3.3641145	8.608794	95-97
TCCGA	619155	3.2923586	7.119997	85-89
GAGGC	362660	3.2064667	11.51948	90-94
ACACA	865085	3.0523908	5.9328837	6
CGAGA	515440	2.9982426	7.893548	85-89
GAGAC	514255	2.99135	7.997344	85-89
AGAGG	467695	2.9506679	8.796789	90-94
ACGAG	493635	2.8714058	7.6772385	95-97
TACAC	800480	2.8003983	7.2359495	5
CATCT	803670	2.7876291	5.1350536	70-74
AAGAG	647725	2.6884983	6.3239884	90-94
CACGA	480805	2.5786283	7.027862	80-84
CAAGA	664450	2.542802	5.8411117	90-94
AGAAG	602165	2.4993937	5.153878	5
ACAAG	621705	2.37922	5.5895495	85-89
AGACA	591130	2.2622118	5.4915724	85-89
GACAA	563695	2.1572201	5.455944	85-89
AGGCA	364955	2.1228926	7.499551	95-97
GGCAA	363795	2.1161447	7.653735	95-97
TATAC	837400	2.0726247	5.590953	5
CTTTG	507240	1.892028	7.9972043	9
AGAGA	441890	1.8341435	5.1880894	8
GAGAG	281470	1.7757822	6.203416	7
TCTCG	326065	1.719095	6.20346	95-97
CAATC	475235	1.6625615	5.0814896	95-97
TGGAG	262835	1.6441017	5.058793	5
GCAAT	398945	1.5137398	5.1796007	95-97
CTCGT	279625	1.4742519	5.9009566	95-97
GTCTT	361125	1.3470125	6.1135635	1
GCCGT	153775	1.2428875	8.272991	95-97
GAGTC	215150	1.2408462	6.079131	9
TGCCG	151775	1.2267227	8.496375	95-97
AAGAC	320265	1.2256309	5.560865	5
GTGTT	302205	1.2226006	5.902946	1
CCGTC	159205	1.1864077	7.0074167	95-97
GATTG	289665	1.1819282	5.4978004	7
ATGCC	221600	1.1783587	5.9137955	95-97
TATGA	428085	1.1491761	5.3376703	4
GACTT	299640	1.1272647	5.7493362	7
GTTCT	299325	1.1164956	5.086195	1
TCATA	429315	1.0625852	5.1620336	2
TGGAC	176030	1.0152273	6.6635556	5
GTGTA	248705	1.0147978	9.13521	1
GGACT	163410	0.9424434	6.255298	6
GTCCA	176395	0.937981	6.4901485	1
AGACT	241120	0.9148955	5.2026305	6
GTATA	260555	0.6994489	6.7663827	1
>>END_MODULE