##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-182_AAGAGGCA-ACTGCATA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2797648	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62663959154261	31.0	31.0	34.0	30.0	34.0
2	31.82689709355859	33.0	31.0	34.0	30.0	34.0
3	31.970446603718553	34.0	31.0	34.0	30.0	34.0
4	35.51232571073988	37.0	35.0	37.0	33.0	37.0
5	35.37490920945022	37.0	35.0	37.0	33.0	37.0
6	35.51584795513946	37.0	35.0	37.0	33.0	37.0
7	35.528421373954124	37.0	35.0	37.0	33.0	37.0
8	35.58187591862879	37.0	35.0	37.0	33.0	37.0
9	37.38398004323632	39.0	37.0	39.0	34.0	39.0
10-14	37.50383364883645	39.2	37.2	39.4	34.0	39.4
15-19	38.542514855335625	40.0	38.0	41.0	34.0	41.0
20-24	38.444440186899854	40.0	38.0	41.0	34.0	41.0
25-29	38.25482476709007	40.0	38.0	41.0	33.6	41.0
30-34	37.988623300715446	40.0	38.0	41.0	33.0	41.0
35-39	37.67213909684135	40.0	37.6	41.0	32.2	41.0
40-44	37.63056360199711	40.0	37.4	41.0	31.8	41.0
45-49	37.478699178738715	40.0	37.0	41.0	31.6	41.0
50-54	36.79270294189977	39.0	36.2	40.2	31.0	40.6
55-59	37.43029366096092	40.0	36.4	41.0	32.0	41.0
60-64	36.98431475296392	39.0	35.4	41.0	31.0	41.0
65-69	36.31811986354252	38.0	35.0	40.2	30.6	41.0
70-74	35.47765229936003	36.6	34.6	39.2	29.6	41.0
75-79	34.50291330431848	35.4	34.0	37.6	29.0	39.6
80-84	33.54754658198601	35.0	33.6	36.4	27.8	38.2
85-89	32.700351795508226	35.0	33.0	35.4	25.8	36.6
90-94	32.04010761897136	35.0	32.2	35.0	24.8	36.0
95-99	31.40870338226968	34.8	31.4	35.0	22.0	35.2
100-101	30.39812388835193	34.0	30.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	56.0
7	261.0
8	620.0
9	783.0
10	950.0
11	1314.0
12	1911.0
13	2712.0
14	3367.0
15	4031.0
16	4806.0
17	5547.0
18	6384.0
19	7303.0
20	8585.0
21	9867.0
22	11266.0
23	12967.0
24	15240.0
25	17746.0
26	20782.0
27	25082.0
28	29842.0
29	36218.0
30	44405.0
31	56048.0
32	73017.0
33	98659.0
34	139356.0
35	213957.0
36	365457.0
37	656739.0
38	766692.0
39	155589.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.162757020289874	23.62629012966999	10.993040048771505	24.217912801268632
2	14.97968180981173	24.018228268117873	39.95673557390619	21.04535434816421
3	15.61557867999977	28.17393008129151	33.23080124482989	22.979689993878825
4	11.28898006992082	18.482500845585687	44.46203945971187	25.76647962478163
5	10.959910180497154	39.17818015389456	38.77914127978489	11.082768385823393
6	25.321963583773083	43.79151551026434	19.630261520682556	11.256259385280021
7	23.20276062830459	34.26407551550887	27.22781903500971	15.305344821176828
8	20.971083538034353	38.12471939993766	24.734520170318877	16.16967689170911
9	22.847781895935746	15.092594214331594	25.500985295804995	36.55863859392766
10-14	20.279213026833673	26.17132397051528	32.98802278893738	20.56144021371367
15-19	19.61393902735597	31.719944371799112	28.98820900375138	19.67790759709354
20-24	19.582654506960225	30.340862898702127	30.39765729376817	19.67882530056948
25-29	19.534534517359766	30.568589765953273	30.146348549640063	19.750527167046897
30-34	19.455771818015638	30.481299211402234	30.217951924911603	19.844977045670525
35-39	19.505475002185882	30.620498341266973	30.19822794422699	19.67579871232015
40-44	19.35302682619652	30.21472468302504	30.58566054517512	19.846587945603318
45-49	19.265297012063048	29.925461722938213	30.81128742499558	19.997953840003156
50-54	18.870744272435196	29.72587187813223	31.14765771446465	20.255726134967922
55-59	18.857502192277266	29.688731659114577	31.112879008743228	20.34088713986493
60-64	18.722282079861927	29.28891268895373	31.181013077245908	20.807792153938436
65-69	19.106678018627207	29.16709088756354	30.564567469140076	21.16166362466918
70-74	19.177260520464205	28.983857339923514	30.42170946786901	21.41717267174327
75-79	19.56745886659707	29.084260329410395	30.133362464533885	21.214918339458656
80-84	19.869151175663273	28.86453597627711	30.049329268937893	21.216983579121727
85-89	20.223011995304258	28.672973888872207	30.0542712806192	21.049742835204338
90-94	20.51119097129502	28.219335959353526	30.29801718674092	20.971455882610535
95-99	21.177247778835106	27.743345084696326	29.910374110872716	21.169033025595855
100-101	21.663612441221876	27.11519485052764	30.08946768541305	21.131725022837433
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.38066982155851
2	36.02503615797594
3	38.5952686738786
4	37.05545969470245
5	22.042678566320546
6	36.5782229690531
7	38.50810544948142
8	37.140760429743466
9	59.40642048986341
10-14	40.84065324054734
15-19	39.291846624449505
20-24	39.26147980752971
25-29	39.285061684406664
30-34	39.30074886368617
35-39	39.181273714506034
40-44	39.19961477179984
45-49	39.26325085206621
50-54	39.12647040740312
55-59	39.1983893321422
60-64	39.53007423380036
65-69	40.26834164329639
70-74	40.594433192207475
75-79	40.78237720605573
80-84	41.086134754785
85-89	41.272754830508596
90-94	41.48264685390555
95-99	42.34628080443096
100-101	42.79533746405931
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	10.0
2	12.0
3	14.0
4	21.0
5	26.5
6	37.0
7	53.5
8	68.0
9	87.0
10	109.5
11	134.5
12	200.5
13	296.0
14	412.0
15	588.0
16	855.5
17	1267.0
18	1903.5
19	2680.0
20	3886.0
21	5737.0
22	8170.5
23	11586.0
24	15791.0
25	20775.5
26	26796.0
27	33605.5
28	41453.5
29	49913.0
30	58554.5
31	68118.5
32	77960.0
33	88146.0
34	98938.5
35	110434.5
36	123508.5
37	135112.0
38	142879.0
39	146350.0
40	145633.0
41	142163.0
42	136683.5
43	130099.0
44	122759.0
45	113887.5
46	103898.0
47	93372.0
48	82034.0
49	70387.5
50	60206.0
51	52075.0
52	44786.5
53	38350.0
54	32564.5
55	27506.5
56	23720.0
57	20012.0
58	16166.5
59	13108.5
60	10588.5
61	8547.0
62	6918.5
63	5082.0
64	3603.5
65	2961.5
66	2379.5
67	1785.5
68	1434.0
69	1399.5
70	1379.0
71	1173.0
72	979.5
73	820.5
74	670.5
75	505.0
76	364.5
77	285.0
78	208.0
79	144.0
80	104.5
81	79.0
82	60.5
83	42.5
84	37.5
85	39.5
86	28.5
87	14.0
88	14.5
89	15.5
90	11.0
91	9.5
92	10.5
93	7.5
94	6.5
95	7.0
96	6.5
97	7.0
98	4.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03334944210279492
2	0.03191966966537606
3	0.02859544874837721
4	0.027523119420313064
5	0.033170720548117566
6	0.001751471235838104
7	8.578634624513163E-4
8	0.002573590387353949
9	3.9318742029018663E-4
10-14	0.006626995247436418
15-19	0.023212355521495198
20-24	0.019337672216090087
25-29	0.001887299617392896
30-34	0.0017657689602122927
35-39	0.005411688675630386
40-44	0.012331787272737671
45-49	0.007606389367068337
50-54	0.004432294555998468
55-59	0.00358872881792134
60-64	0.009650963952577309
65-69	0.012982333731763253
70-74	0.017007143143097345
75-79	0.00676282362899121
80-84	0.010094193408177155
85-89	0.007863748405803731
90-94	0.009257776532287121
95-99	0.008650123246384105
100-101	0.005629728972336763
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2797648.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.32409190091131
#Duplication Level	Relative count
1	100.0
2	8.204218435397534
3	2.500859401856308
4	1.5659529538869519
5	1.2203506359573737
6	1.0177773412562
7	0.8618572901831754
8	0.7206698423611452
9	0.6623532878259588
10++	6.017654569562442
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	580295	4.2947307	9.832515	85-89
GTCTC	874075	4.201662	6.775377	60-64
GACGC	533325	4.0879455	9.914435	75-79
CTGTC	824245	3.96213	6.5531697	55-59
CGCTG	532055	3.9377089	9.694186	75-79
GCCGA	493780	3.7848327	10.174417	80-84
CTGCC	522580	3.726267	9.444872	80-84
CGACG	485880	3.724279	10.082396	95-97
TCTCT	1188550	3.710866	5.495765	60-64
TGCCG	482255	3.5691416	9.654436	80-84
CCGAC	475770	3.5135357	9.6142235	80-84
CTCTT	1096400	3.4231572	5.1644506	60-64
TGTCT	984375	3.1899524	4.897417	55-59
ACACA	911390	3.1611154	5.9458675	6
CTGAC	610955	3.0416396	6.505112	70-74
TGACG	585235	3.0240896	6.8295894	75-79
GACGA	536025	2.8686361	7.4131207	85-89
ATCTG	832970	2.795626	5.281649	70-74
TACAC	827645	2.7717505	7.151285	5
ACGCT	542190	2.699293	6.5747304	75-79
AGAAG	659270	2.463378	5.4122963	5
TGCAG	463800	2.3965974	7.1407194	90-94
TATAC	879220	1.9849921	5.262337	5
GTGTG	378040	1.9576803	6.649264	95-97
AGAGA	520675	1.9455144	5.2695227	8
GCAGT	375930	1.942546	6.594903	90-94
CAGTG	370450	1.9142292	6.5805902	95-97
GGTGG	236880	1.8886307	7.611639	95-97
GAGAG	330300	1.8346992	6.1255927	7
CTTTG	534625	1.7324985	7.0170193	9
CTCGG	211270	1.5635972	7.19	95-97
GTGTA	426015	1.4840221	8.246496	1
CGGTG	171485	1.3172828	6.862833	95-97
AAGAC	347055	1.2493963	5.530623	5
GAGTC	225315	1.164272	5.202347	9
GTCTT	348505	1.1293606	5.8023214	1
GTGTT	325035	1.0932502	5.4154034	1
GTTCT	335245	1.0863904	5.076049	1
GACTT	319645	1.0727972	5.0016537	7
TGGAC	188500	0.9740375	5.8253837	5
GTCCA	188800	0.9399408	6.2236776	1
GGACT	170370	0.8803542	5.2497435	6
GTATA	285435	0.6688585	6.745415	1
>>END_MODULE