##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-182_AAGAGGCA-ACTGCATA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2797648	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46388609288945	34.0	31.0	34.0	31.0	34.0
2	32.648919377991795	34.0	31.0	34.0	31.0	34.0
3	32.759911182536186	34.0	31.0	34.0	31.0	34.0
4	36.272059244050716	37.0	37.0	37.0	35.0	37.0
5	35.9985137515513	37.0	35.0	37.0	35.0	37.0
6	36.08483125825693	37.0	36.0	37.0	35.0	37.0
7	36.08608052192413	37.0	36.0	37.0	35.0	37.0
8	36.11049495862238	37.0	36.0	37.0	35.0	37.0
9	37.983127970352236	39.0	38.0	39.0	35.0	39.0
10-14	38.17135765471568	39.4	38.2	39.4	35.2	39.4
15-19	39.308554042538596	40.8	39.0	41.0	36.0	41.0
20-24	39.24487934150401	40.0	39.0	41.0	36.0	41.0
25-29	39.05268582752369	40.0	39.0	41.0	36.0	41.0
30-34	38.85707308424791	40.0	38.0	41.0	35.0	41.0
35-39	38.6318694131642	40.0	38.0	41.0	34.8	41.0
40-44	38.39657355035373	40.0	38.0	41.0	34.0	41.0
45-49	38.22443309522856	40.0	38.0	41.0	33.4	41.0
50-54	38.175668061171386	40.0	37.6	41.0	33.6	41.0
55-59	37.80422126014424	39.8	36.6	41.0	33.0	41.0
60-64	37.250962880247975	39.0	35.6	40.8	32.4	41.0
65-69	36.51307090813426	37.8	35.0	40.0	31.0	41.0
70-74	35.91424410790779	36.6	35.0	39.2	31.0	41.0
75-79	34.69665397505334	35.2	34.0	37.4	30.4	39.2
80-84	34.464848329739844	35.0	34.0	36.4	31.0	38.4
85-89	33.82401846122171	35.0	34.0	35.6	30.0	36.8
90-94	33.40856562369533	35.0	34.0	35.0	29.8	36.0
95-99	33.125801244473934	35.0	33.8	35.0	29.0	35.4
100-101	32.77828447324324	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	12.0
14	40.0
15	94.0
16	233.0
17	510.0
18	932.0
19	1456.0
20	2288.0
21	3195.0
22	4531.0
23	6144.0
24	8431.0
25	11034.0
26	14114.0
27	18022.0
28	23108.0
29	29027.0
30	36606.0
31	46497.0
32	60261.0
33	80058.0
34	115733.0
35	184756.0
36	341575.0
37	683487.0
38	920253.0
39	205066.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.70419152087754	23.668989093695846	11.285193848547065	24.341625536879548
2	15.181676485728993	23.90961934450609	39.76287679460047	21.145827375164444
3	15.751838687354521	27.876916609952364	33.40741937513225	22.963825327560865
4	11.431674034760627	18.505830611999794	44.21338924696745	25.849106106272124
5	10.82427095903416	39.28835936472351	38.897352347400385	10.990017328841942
6	25.25682287407136	44.023122279857944	19.544881986583015	11.175172859487684
7	23.148230227676965	34.28512093015276	27.19663088422847	15.370017957941814
8	20.980587979617162	38.24962968893871	24.629295751288225	16.1404865801559
9	22.797042372735955	14.918817521003358	25.4285742881163	36.855565818144385
10-14	20.224216913636024	26.159924336442614	33.00453809771637	20.611320652204995
15-19	19.54857294618956	31.815863286504193	28.921414189290385	19.71414957801586
20-24	19.53944449126979	30.349472206869944	30.422312427139225	19.68877087472104
25-29	19.540503891280807	30.618868984906427	30.088786769109	19.751840354703766
30-34	19.412277944865956	30.50837145603309	30.172116232362917	19.907234366738034
35-39	19.51381843257738	30.595470004647314	30.174908702208924	19.715802860566377
40-44	19.325365887851138	30.19689494215746	30.575256811715278	19.902482358276124
45-49	19.235420335494773	29.921894507552878	30.812527005471658	20.030158151480695
50-54	18.810874518829607	29.720426572779342	31.148827974852182	20.31987093353887
55-59	18.73073024220016	29.605979001318943	31.099570731838494	20.563720024642404
60-64	18.471927603355702	29.27358807238663	31.003516305030825	21.250968019226846
65-69	18.74172701694862	29.365224219832847	30.158808375756134	21.7342403874624
70-74	18.756465904740526	29.444640845823088	29.676950512929306	22.121942736507076
75-79	19.223585624468207	29.902689768106285	28.910220912913783	21.963503694511726
80-84	19.28925170223649	30.042775293345148	28.413046267876208	22.254926736542153
85-89	19.360209038558462	29.918028860761304	28.35171237895745	22.370049721722783
90-94	19.411851797942838	29.40036115492079	28.596073335652957	22.591713711483415
95-99	19.50571254993754	29.005537185013353	28.548587522396023	22.94016274265308
100-101	19.426289306692375	28.22484394921875	29.201378873031516	23.147487871057358
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.04581705775708
2	36.32750386089344
3	38.71566401491538
4	37.28078014103276
5	21.814288287876103
6	36.43199573355905
7	38.51824818561877
8	37.121074559773064
9	59.65260819088034
10-14	40.835537565841015
15-19	39.26272252420542
20-24	39.228215365990835
25-29	39.29234424598457
30-34	39.31951231160399
35-39	39.22962129314376
40-44	39.22784824612726
45-49	39.265578486975464
50-54	39.13074545236847
55-59	39.29445026684256
60-64	39.72289562258254
65-69	40.475967404411016
70-74	40.8784086412476
75-79	41.187089318979936
80-84	41.544178438778644
85-89	41.73025876028124
90-94	42.00356550942625
95-99	42.44587529259062
100-101	42.57377717774973
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	1.0
6	5.0
7	8.5
8	13.0
9	23.0
10	41.5
11	68.5
12	109.0
13	189.5
14	310.5
15	479.0
16	758.5
17	1163.0
18	1759.0
19	2587.5
20	3853.5
21	5765.0
22	8338.0
23	11730.0
24	15694.0
25	20689.5
26	26813.0
27	33525.5
28	41054.0
29	49216.0
30	57961.5
31	67427.0
32	76818.5
33	86528.0
34	97520.5
35	108868.0
36	121144.0
37	132879.5
38	140882.5
39	143798.0
40	142446.5
41	139273.0
42	134300.5
43	128027.0
44	121939.5
45	114387.5
46	106484.5
47	97527.5
48	86251.5
49	74432.0
50	63396.0
51	54894.5
52	47264.0
53	40306.0
54	34046.5
55	28564.0
56	24669.0
57	20604.5
58	16362.0
59	13399.0
60	10815.0
61	8573.0
62	6906.0
63	4990.5
64	3582.0
65	2873.5
66	2234.0
67	1717.5
68	1407.0
69	1413.5
70	1383.0
71	1164.0
72	1012.0
73	807.0
74	612.0
75	515.0
76	375.5
77	240.0
78	148.0
79	90.0
80	59.0
81	39.5
82	31.5
83	24.5
84	14.0
85	10.0
86	5.5
87	3.0
88	2.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013153906424253516
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.001108073638999617
20-24	6.362487346513928E-4
25-29	0.017764922534929343
30-34	0.006777121353365399
35-39	0.04270730270570136
40-44	0.05132883050333709
45-49	0.05202227013548523
50-54	0.051657678163943434
55-59	0.041735057448256535
60-64	0.0526942631810721
65-69	0.04275019587882393
70-74	0.0031812436732569643
75-79	0.0013868792642962946
80-84	1.7872155467735755E-4
85-89	0.002788056252966778
90-94	0.00240916655705078
95-99	0.0019015973417670843
100-101	3.574431093547151E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2797648.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.86963477624903
#Duplication Level	Relative count
1	100.0
2	8.263912440265146
3	2.5077848003699708
4	1.6432865731462927
5	1.2326190843224911
6	1.0235856328040698
7	0.9489748728225682
8	0.7584399568367505
9	0.6320332973639586
10++	6.312933559426545
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	601415	4.509418	10.10378	75-79
GAGCC	582755	4.369505	10.073241	75-79
CTCCG	621750	4.2798133	9.034197	70-74
CGAGC	564505	4.232667	9.964178	75-79
GTCTC	873785	4.215232	6.7974863	60-64
CTGTC	826985	3.9894638	6.5856304	55-59
AGCCC	551890	3.8273396	9.059975	80-84
GCCCA	547390	3.7961323	9.257018	80-84
TCTCT	1189355	3.7190716	5.5705385	60-64
TCTCC	795550	3.5496264	6.643842	70-74
CTCTT	1094590	3.4227445	5.20779	60-64
CCACG	489675	3.39588	9.115792	80-84
CCCAC	526430	3.376628	8.5005045	90-94
TGTCT	988790	3.3429422	5.1274085	55-59
ATCTC	1043255	3.2866154	8.1247425	95-97
TCCGA	652925	3.1733334	6.6830764	75-79
GAGGC	387105	3.138173	10.783207	95-97
GAGAC	555350	2.9400613	7.528058	85-89
ACACA	913545	2.921186	5.7084885	6
CGAGA	551130	2.9177206	7.4561815	85-89
AGAGG	508390	2.9099696	8.206238	90-94
ACGAG	524740	2.7780097	7.1077647	80-84
AAGAG	718695	2.6864426	5.906742	90-94
TACAC	832365	2.6418467	6.9218364	5
CAAGA	730830	2.526664	5.5647745	90-94
CACGA	510820	2.5012417	6.5559793	80-84
AGAAG	658270	2.4605772	5.2594266	5
ACAAG	674240	2.331018	5.2001204	85-89
AGACA	635600	2.1974294	5.129377	85-89
GACAA	611030	2.1124852	5.090821	85-89
AGGCA	393995	2.0858366	7.066706	95-97
GGCAA	389200	2.0604517	7.1514153	95-97
TATAC	884805	1.9681097	5.530416	5
AGAGA	522220	1.9520297	5.413476	8
GAGAG	329735	1.8873676	6.2886786	7
CTTTG	534005	1.8053867	7.222332	9
TCTCG	354145	1.7084336	5.8452687	95-97
GCTTT	448145	1.5151072	5.0510626	1
CTCGT	306630	1.4792161	5.655929	90-94
AGGAG	253815	1.4528097	5.012071	7
GTCTT	395310	1.3364804	5.8248706	1
GCCGT	165590	1.2323811	8.386259	95-97
TGCCG	163625	1.2177567	8.679754	95-97
AAGAC	346615	1.1983354	5.3723855	5
ATGCC	246535	1.1982045	5.9526033	95-97
CCGTC	172820	1.1896057	7.1835613	95-97
GAGTC	226050	1.1878421	5.177093	9
GTGTT	324475	1.1860635	5.858203	1
GATTG	319595	1.1769611	5.128078	7
GACTT	317045	1.0798942	5.1063175	7
TGGAC	188505	0.9905515	6.507035	5
GTGTA	258835	0.95320225	8.757473	1
GTCCA	190895	0.9277841	6.1959686	1
GGACT	169055	0.8883461	5.5133996	6
GTATA	285330	0.68620044	6.622801	1
>>END_MODULE