FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | Nextera-181_AAGAGGCA-GTAAGGAG_L005_R2_001.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 1653252 |
| Filtered Sequences | 0 |
| Sequence length | 101 |
| %GC | 40 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
![[WARN]](Icons/warning.png)
Sequence Duplication Levels
Overrepresented sequences
No overrepresented sequences
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| GACGC | 402040 | 5.1217647 | 13.1027155 | 75-79 |
| GCTGC | 414220 | 5.0296383 | 12.564789 | 80-84 |
| GTCTC | 633060 | 4.9074435 | 8.400333 | 60-64 |
| CGCTG | 393860 | 4.782418 | 12.482794 | 75-79 |
| CTGTC | 610000 | 4.7286835 | 8.222171 | 55-59 |
| GCCGA | 359370 | 4.5781727 | 13.162787 | 80-84 |
| CGACG | 347635 | 4.4286757 | 13.093488 | 95-97 |
| TGCCG | 356855 | 4.333088 | 12.393975 | 80-84 |
| CTGCC | 383415 | 4.2745657 | 11.656513 | 80-84 |
| CCGAC | 347925 | 4.069614 | 11.881251 | 80-84 |
| TCTCT | 810460 | 4.0109453 | 6.435311 | 60-64 |
| TGACG | 431510 | 3.8223357 | 9.264446 | 75-79 |
| TGTCT | 706715 | 3.809275 | 6.1541576 | 55-59 |
| CTCTT | 756310 | 3.7429583 | 6.1285706 | 60-64 |
| ACACA | 645785 | 3.6909416 | 6.7914824 | 70-74 |
| CTGAC | 449355 | 3.6546407 | 8.384726 | 75-79 |
| GACGA | 371005 | 3.4479616 | 9.815138 | 85-89 |
| ATCTG | 596355 | 3.3724658 | 6.7873 | 70-74 |
| TACAC | 603945 | 3.2900457 | 7.6291575 | 5 |
| ACGCT | 403020 | 3.2777946 | 8.423384 | 75-79 |
| CACAT | 592410 | 3.2272077 | 5.998159 | 70-74 |
| TCTGA | 556000 | 3.1442528 | 6.326511 | 70-74 |
| CATCT | 601240 | 3.1218193 | 6.0424333 | 70-74 |
| ACATC | 571835 | 3.1151235 | 6.144005 | 70-74 |
| GACTC | 377030 | 3.0664155 | 8.833289 | 85-89 |
| ACGAC | 323090 | 2.7569149 | 8.674912 | 95-97 |
| CGACT | 327350 | 2.6623642 | 8.035713 | 85-89 |
| AGAAG | 390765 | 2.6492963 | 5.762013 | 5 |
| CTCCT | 369150 | 2.627426 | 7.478324 | 90-94 |
| ACTCC | 349865 | 2.6125994 | 7.417205 | 90-94 |
| TATAC | 631770 | 2.3930464 | 5.633646 | 5 |
| TCCTT | 431385 | 2.1349132 | 5.413483 | 90-94 |
| CAAAG | 329445 | 2.0507605 | 5.477654 | 4 |
| GGTGG | 138670 | 1.9973485 | 9.184616 | 95-97 |
| AGAGA | 291860 | 1.9787433 | 5.6323004 | 8 |
| GAGAG | 185730 | 1.8799553 | 6.857548 | 7 |
| ACGTG | 206810 | 1.8319328 | 8.223447 | 95-97 |
| CTTTG | 325050 | 1.7520566 | 7.5308557 | 9 |
| GTGTA | 282150 | 1.737823 | 9.357858 | 1 |
| AAGAG | 248205 | 1.6827725 | 5.3541994 | 5 |
| CGTGT | 195365 | 1.6494533 | 7.539127 | 95-97 |
| CTTAC | 306410 | 1.5909729 | 5.213198 | 95-97 |
| TGTAG | 256400 | 1.5792233 | 5.197154 | 95-97 |
| CTCGG | 127705 | 1.5506494 | 8.690965 | 95-97 |
| CCTTA | 295935 | 1.5365837 | 5.1377225 | 90-94 |
| TTACG | 257790 | 1.4578364 | 5.5242863 | 95-97 |
| AGGAG | 143280 | 1.4502772 | 5.394736 | 7 |
| GTAGA | 219075 | 1.4156733 | 5.039579 | 90-94 |
| CGGTG | 102410 | 1.35435 | 8.206279 | 95-97 |
| AAGAC | 211475 | 1.3164097 | 6.045349 | 5 |
| TCTCG | 168760 | 1.3082174 | 5.602356 | 95-97 |
| TACGT | 223715 | 1.2651376 | 5.3633666 | 95-97 |
| GAGTC | 140700 | 1.2463272 | 6.4522467 | 9 |
| AGAAC | 195050 | 1.2141658 | 5.148512 | 5 |
| TATGA | 279755 | 1.1541249 | 5.6555176 | 4 |
| GATTG | 185840 | 1.144629 | 5.4900723 | 7 |
| GTCTT | 208090 | 1.121629 | 6.102785 | 1 |
| GTGTT | 187685 | 1.1018193 | 5.661433 | 1 |
| GACTT | 191845 | 1.0849086 | 5.7675176 | 7 |
| TCATA | 283125 | 1.0724334 | 5.284416 | 2 |
| TGGAC | 114575 | 1.0149107 | 6.772038 | 5 |
| TCGGT | 117745 | 0.99411297 | 5.3714404 | 95-97 |
| AGACT | 157615 | 0.9351578 | 5.334515 | 6 |
| GTCCA | 114530 | 0.9314819 | 6.841218 | 1 |
| GGACT | 104010 | 0.9213255 | 6.172947 | 6 |
| GAGTA | 139490 | 0.9013911 | 5.2757416 | 1 |
| GAATA | 205770 | 0.8906388 | 5.011886 | 1 |
| GGATA | 130025 | 0.84022784 | 5.1158705 | 1 |
| GTATA | 169155 | 0.69784623 | 7.0304356 | 1 |