##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-181_AAGAGGCA-GTAAGGAG_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1653252	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.626139874622865	31.0	31.0	34.0	30.0	34.0
2	31.82753324961954	33.0	31.0	34.0	30.0	34.0
3	31.96620357936963	34.0	31.0	34.0	30.0	34.0
4	35.512737925010825	37.0	35.0	37.0	33.0	37.0
5	35.37776818053146	37.0	35.0	37.0	33.0	37.0
6	35.51343775782518	37.0	35.0	37.0	33.0	37.0
7	35.532907263986374	37.0	35.0	37.0	33.0	37.0
8	35.58634436855361	37.0	35.0	37.0	33.0	37.0
9	37.392161630531824	39.0	37.0	39.0	34.0	39.0
10-14	37.50674331559859	39.2	37.2	39.4	34.0	39.4
15-19	38.539521500654466	40.0	38.0	41.0	34.0	41.0
20-24	38.44553594975237	40.0	38.0	41.0	34.0	41.0
25-29	38.26397052596942	40.0	38.0	41.0	33.6	41.0
30-34	38.00249217905075	40.0	38.0	41.0	33.0	41.0
35-39	37.69329745253598	40.0	37.6	41.0	32.2	41.0
40-44	37.65883289419883	40.0	37.6	41.0	31.8	41.0
45-49	37.51118317110761	40.0	37.0	41.0	31.6	41.0
50-54	36.82622177381306	39.0	36.4	40.2	31.0	40.6
55-59	37.487524708876805	40.0	36.6	41.0	32.0	41.0
60-64	37.04793546295422	39.0	35.6	41.0	31.0	41.0
65-69	36.37624838802554	38.2	35.0	40.2	30.6	41.0
70-74	35.486901210462776	36.6	34.6	39.2	29.4	41.0
75-79	34.430656185505896	35.4	34.0	37.6	28.8	39.4
80-84	33.39900136216379	35.0	33.2	36.4	26.8	38.2
85-89	32.48200189686751	35.0	32.6	35.4	25.4	36.6
90-94	31.73076106969778	35.0	31.8	35.0	23.8	36.0
95-99	31.004274968365383	34.2	30.8	35.0	19.8	35.0
100-101	29.900449840677645	34.0	28.5	35.0	16.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	29.0
7	172.0
8	311.0
9	492.0
10	581.0
11	715.0
12	1047.0
13	1496.0
14	1821.0
15	2280.0
16	2714.0
17	3230.0
18	3792.0
19	4402.0
20	5089.0
21	5772.0
22	6865.0
23	7810.0
24	9178.0
25	10717.0
26	12741.0
27	15347.0
28	18552.0
29	22185.0
30	27375.0
31	34632.0
32	44748.0
33	60048.0
34	84690.0
35	127390.0
36	217216.0
37	388974.0
38	445316.0
39	85486.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.796603308470495	23.78143933411873	11.150703680777797	24.27125367663298
2	14.851643304979834	24.33088522701557	40.06435506375605	20.753116404248548
3	15.383665927300052	28.59945085995235	33.20476098854889	22.81212222419871
4	10.98724573143431	18.723181548663465	44.80959353331962	25.479979186582607
5	10.594020525362637	39.606410651260646	39.13596464424298	10.663604179133738
6	24.959881443844605	44.38524701719367	19.737482798772096	10.91738874018963
7	23.004747655659774	34.39283055000584	27.524970708978806	15.077451085355579
8	20.690095282338653	38.700042100373096	24.73015838450706	15.879704232781188
9	23.03549869226805	15.094021209210498	25.152730026541757	36.717750071979694
10-14	20.216469311797784	26.135228049765463	33.18081864564344	20.46748399279331
15-19	19.589795329647725	31.77075720189308	28.95320015836953	19.68624731008967
20-24	19.571969383931297	30.32763191312678	30.44229031735428	19.658108385587646
25-29	19.51527913422534	30.646992521361703	30.07623809940314	19.761490245009817
30-34	19.422976163645338	30.588752504061485	30.12201322732408	19.866258104969095
35-39	19.49556875998473	30.72786542531859	30.11475674606463	19.661809068632046
40-44	19.269382714017947	30.2098401625718	30.616245468320713	19.90453165508954
45-49	19.216292096089386	29.801027251167923	30.89973158483993	20.08294906790277
50-54	18.70339760093484	29.47376074653606	31.396562439245162	20.426279213283934
55-59	18.632466513886165	29.507594403941305	31.3716528252436	20.488286256928927
60-64	18.418436136480665	28.97200320953336	31.469844808028093	21.139715845957884
65-69	18.91485134235553	28.765091472599597	30.671626640119694	21.64843054492518
70-74	18.920638692627175	28.2786138444707	30.353605029935544	22.44714243296658
75-79	18.9951720231408	28.240640032985002	29.97391348743596	22.790274456438233
80-84	19.005627133162577	27.78122054708172	29.929948181011966	23.283204138743738
85-89	19.2798316269544	27.636587745431108	29.857977587685426	23.225603039929073
90-94	19.562341847385866	27.011955324362958	30.149025661934665	23.27667716631651
95-99	20.353966026675455	26.424905382977236	29.742883185936215	23.478245404411098
100-101	21.016343423613943	25.599283062193084	29.85424119646104	23.53013231773193
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.06785698510346
2	35.604759709228375
3	38.19578815149876
4	36.46722491801692
5	21.257624704496376
6	35.87727018403424
7	38.082198741015354
8	36.56979951511984
9	59.75324876424774
10-14	40.6839533045911
15-19	39.27604263973739
20-24	39.23007776951894
25-29	39.276769379235155
30-34	39.289234268614436
35-39	39.15737782861678
40-44	39.173914369107486
45-49	39.29924116399216
50-54	39.129676814218776
55-59	39.120752770815095
60-64	39.55815198243855
65-69	40.5632818872807
70-74	41.36778112559376
75-79	41.785446479579036
80-84	42.28883127190632
85-89	42.505434666883474
90-94	42.83901901370238
95-99	43.832211431086556
100-101	44.54647574134587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	1.5
3	3.5
4	8.5
5	11.5
6	15.5
7	20.0
8	21.0
9	29.0
10	44.0
11	63.0
12	82.0
13	125.5
14	188.5
15	261.0
16	382.0
17	564.0
18	792.5
19	1143.5
20	1677.5
21	2427.0
22	3516.5
23	5147.0
24	7175.5
25	9802.5
26	13164.0
27	17015.0
28	21379.0
29	26171.5
30	31429.5
31	36924.0
32	42814.0
33	49070.5
34	55708.5
35	63391.0
36	72229.5
37	80558.0
38	86869.0
39	89737.0
40	89221.5
41	86471.5
42	82634.0
43	78891.5
44	75238.5
45	71029.0
46	65466.0
47	59078.5
48	52283.5
49	45299.5
50	38842.0
51	33299.5
52	28227.0
53	24025.0
54	20293.5
55	17051.0
56	14435.0
57	11617.5
58	9137.0
59	7257.0
60	5645.5
61	4453.0
62	3454.5
63	2337.5
64	1549.5
65	1184.5
66	921.0
67	661.0
68	506.0
69	473.5
70	473.0
71	407.5
72	338.0
73	274.5
74	199.0
75	148.0
76	103.5
77	75.5
78	53.5
79	33.0
80	27.5
81	26.0
82	25.0
83	19.5
84	11.0
85	13.0
86	13.0
87	7.0
88	5.0
89	5.0
90	5.0
91	3.0
92	1.5
93	4.0
94	6.5
95	7.5
96	6.5
97	4.5
98	4.0
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035384805220256804
2	0.033025818205573015
3	0.029396607413751805
4	0.028549791562326857
5	0.03417506828964973
6	0.0016331448563195448
7	9.073026979553026E-4
8	0.0036292107918212105
9	4.838947722428281E-4
10-14	0.007355200538090988
15-19	0.024727022861608515
20-24	0.02069859888268697
25-29	0.0022138185830109383
30-34	0.0022864027988473627
35-39	0.006230145192626411
40-44	0.013452274668350622
45-49	0.008540742730085916
50-54	0.005528497772874311
55-59	0.0044881240125522305
60-64	0.0106940744665665
65-69	0.014057143133654155
70-74	0.018170248697718196
75-79	0.008298795343964502
80-84	0.011492500840767167
85-89	0.009726284922080844
90-94	0.010948119221993985
95-99	0.010089206001262965
100-101	0.006653553118338886
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1653252.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	24.595055220770067
#Duplication Level	Relative count
1	100.0
2	7.110996534730632
3	2.5731723401959257
4	1.7626110624827636
5	1.3285531001570763
6	1.067159078646027
7	0.8441348217604527
8	0.6978500941258289
9	0.5743474142375807
10++	3.9466900082734804
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GACGC	402040	5.1217647	13.1027155	75-79
GCTGC	414220	5.0296383	12.564789	80-84
GTCTC	633060	4.9074435	8.400333	60-64
CGCTG	393860	4.782418	12.482794	75-79
CTGTC	610000	4.7286835	8.222171	55-59
GCCGA	359370	4.5781727	13.162787	80-84
CGACG	347635	4.4286757	13.093488	95-97
TGCCG	356855	4.333088	12.393975	80-84
CTGCC	383415	4.2745657	11.656513	80-84
CCGAC	347925	4.069614	11.881251	80-84
TCTCT	810460	4.0109453	6.435311	60-64
TGACG	431510	3.8223357	9.264446	75-79
TGTCT	706715	3.809275	6.1541576	55-59
CTCTT	756310	3.7429583	6.1285706	60-64
ACACA	645785	3.6909416	6.7914824	70-74
CTGAC	449355	3.6546407	8.384726	75-79
GACGA	371005	3.4479616	9.815138	85-89
ATCTG	596355	3.3724658	6.7873	70-74
TACAC	603945	3.2900457	7.6291575	5
ACGCT	403020	3.2777946	8.423384	75-79
CACAT	592410	3.2272077	5.998159	70-74
TCTGA	556000	3.1442528	6.326511	70-74
CATCT	601240	3.1218193	6.0424333	70-74
ACATC	571835	3.1151235	6.144005	70-74
GACTC	377030	3.0664155	8.833289	85-89
ACGAC	323090	2.7569149	8.674912	95-97
CGACT	327350	2.6623642	8.035713	85-89
AGAAG	390765	2.6492963	5.762013	5
CTCCT	369150	2.627426	7.478324	90-94
ACTCC	349865	2.6125994	7.417205	90-94
TATAC	631770	2.3930464	5.633646	5
TCCTT	431385	2.1349132	5.413483	90-94
CAAAG	329445	2.0507605	5.477654	4
GGTGG	138670	1.9973485	9.184616	95-97
AGAGA	291860	1.9787433	5.6323004	8
GAGAG	185730	1.8799553	6.857548	7
ACGTG	206810	1.8319328	8.223447	95-97
CTTTG	325050	1.7520566	7.5308557	9
GTGTA	282150	1.737823	9.357858	1
AAGAG	248205	1.6827725	5.3541994	5
CGTGT	195365	1.6494533	7.539127	95-97
CTTAC	306410	1.5909729	5.213198	95-97
TGTAG	256400	1.5792233	5.197154	95-97
CTCGG	127705	1.5506494	8.690965	95-97
CCTTA	295935	1.5365837	5.1377225	90-94
TTACG	257790	1.4578364	5.5242863	95-97
AGGAG	143280	1.4502772	5.394736	7
GTAGA	219075	1.4156733	5.039579	90-94
CGGTG	102410	1.35435	8.206279	95-97
AAGAC	211475	1.3164097	6.045349	5
TCTCG	168760	1.3082174	5.602356	95-97
TACGT	223715	1.2651376	5.3633666	95-97
GAGTC	140700	1.2463272	6.4522467	9
AGAAC	195050	1.2141658	5.148512	5
TATGA	279755	1.1541249	5.6555176	4
GATTG	185840	1.144629	5.4900723	7
GTCTT	208090	1.121629	6.102785	1
GTGTT	187685	1.1018193	5.661433	1
GACTT	191845	1.0849086	5.7675176	7
TCATA	283125	1.0724334	5.284416	2
TGGAC	114575	1.0149107	6.772038	5
TCGGT	117745	0.99411297	5.3714404	95-97
AGACT	157615	0.9351578	5.334515	6
GTCCA	114530	0.9314819	6.841218	1
GGACT	104010	0.9213255	6.172947	6
GAGTA	139490	0.9013911	5.2757416	1
GAATA	205770	0.8906388	5.011886	1
GGATA	130025	0.84022784	5.1158705	1
GTATA	169155	0.69784623	7.0304356	1
>>END_MODULE