##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-181_AAGAGGCA-GTAAGGAG_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1653252	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44936585590098	34.0	31.0	34.0	31.0	34.0
2	32.631767570824046	34.0	31.0	34.0	31.0	34.0
3	32.74233813115	34.0	31.0	34.0	31.0	34.0
4	36.26063661196236	37.0	37.0	37.0	35.0	37.0
5	35.98723969485596	37.0	35.0	37.0	35.0	37.0
6	36.07431595425259	37.0	36.0	37.0	35.0	37.0
7	36.07148819417729	37.0	36.0	37.0	35.0	37.0
8	36.094101201752665	37.0	36.0	37.0	35.0	37.0
9	37.96522792653509	39.0	38.0	39.0	35.0	39.0
10-14	38.15130772562198	39.4	38.2	39.4	35.2	39.4
15-19	39.282070609925164	40.6	39.0	41.0	36.0	41.0
20-24	39.219393413708254	40.0	39.0	41.0	36.0	41.0
25-29	39.03159482039036	40.0	39.0	41.0	36.0	41.0
30-34	38.83743954339689	40.0	38.0	41.0	35.0	41.0
35-39	38.62059274690126	40.0	38.0	41.0	34.8	41.0
40-44	38.39430721995195	40.0	38.0	41.0	34.0	41.0
45-49	38.23363032374979	40.0	38.0	41.0	33.6	41.0
50-54	38.1998308787771	40.0	37.6	41.0	33.6	41.0
55-59	37.842251513985765	40.0	36.8	41.0	33.0	41.0
60-64	37.29490737044323	39.0	35.8	40.8	32.4	41.0
65-69	36.554673697657705	37.8	35.0	40.0	31.0	41.0
70-74	35.936475383063204	36.6	35.0	39.2	31.0	41.0
75-79	34.688883364423575	35.2	34.0	37.4	30.4	39.2
80-84	34.43204931855519	35.0	34.0	36.4	31.0	38.2
85-89	33.78228146707217	35.0	34.0	35.4	30.0	36.8
90-94	33.354423282113075	35.0	34.0	35.0	29.4	36.0
95-99	33.07651332041334	35.0	33.4	35.0	29.0	35.2
100-101	32.71875340238512	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	21.0
15	51.0
16	156.0
17	314.0
18	564.0
19	818.0
20	1342.0
21	1838.0
22	2760.0
23	3707.0
24	5116.0
25	6566.0
26	8573.0
27	11051.0
28	13881.0
29	17458.0
30	22004.0
31	27678.0
32	35676.0
33	47827.0
34	68015.0
35	107109.0
36	199438.0
37	404454.0
38	548365.0
39	118381.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.441082182268644	23.74036142100539	11.425662875351126	24.39289352137484
2	15.055561369769702	24.162375070854434	39.90981448086159	20.872249078514272
3	15.489532146339457	28.221650419899692	33.50590230648443	22.782915127276425
4	11.099880719938641	18.685536143310273	44.60033921023534	25.61424392651574
5	10.51944894063337	39.583499672161295	39.267395412193665	10.629655975011675
6	24.956509957344675	44.56688998410406	19.59477442035455	10.881825638196718
7	22.93461613837455	34.41223721489525	27.54044755427485	15.112699092455353
8	20.67960601287644	38.80382422038504	24.656434711707593	15.860135055030932
9	22.94199553365125	14.896640076648932	25.164887143641746	36.99647724605807
10-14	20.17659739713002	26.03252256764244	33.252776951124204	20.538103084103334
15-19	19.558352065289675	31.766087273475996	28.970949932477858	19.704610728756474
20-24	19.54708357704654	30.299913926644695	30.476439914083915	19.676562582224854
25-29	19.510229989335517	30.658763447664544	30.077552826934607	19.75345373606533
30-34	19.387574122704397	30.579143703257056	30.14360816512991	19.889674008908635
35-39	19.508655007407306	30.681299014170637	30.116029829637426	19.69401614878463
40-44	19.264773288575935	30.158533811876342	30.656172223195217	19.92052067635251
45-49	19.215635011988667	29.757769636055325	30.939933212054754	20.08666213990126
50-54	18.670420394068756	29.44059617453864	31.391671034046194	20.49731239734641
55-59	18.488615071514793	29.354080703725604	31.40930046911652	20.748003755643087
60-64	18.140374664406156	28.87180070025504	31.294624555501937	21.69320007983687
65-69	18.445977183992486	28.954472068181765	30.222822785024604	22.376727962801148
70-74	18.455180019583995	29.072334717407617	29.538089706498987	22.934395556509404
75-79	19.028924829992707	29.67963430467223	28.504850403744637	22.786590461590425
80-84	19.166286405288787	29.928695547820748	27.80876054469237	23.096257502198096
85-89	19.312258701563533	29.796465188483317	27.632505225952663	23.258770884000484
90-94	19.386719757987198	29.207601878972067	27.887533149310627	23.518145213730108
95-99	19.538805558828646	28.635015626553795	27.891493139777147	23.934685674840413
100-101	19.498158476843503	27.726340404451594	28.65387651360864	24.12162460509626
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	64.83397570364349
2	35.92781044828398
3	38.27244727361588
4	36.71412464645438
5	21.149104915645044
6	35.838335595541395
7	38.0473152308299
8	36.53974106790737
9	59.93847277970933
10-14	40.71470048123335
15-19	39.262962794046146
20-24	39.2236461592714
25-29	39.26368372540085
30-34	39.277248131613035
35-39	39.20267115619194
40-44	39.18529396492844
45-49	39.302297151889924
50-54	39.16773279141517
55-59	39.236618827157876
60-64	39.83357474424302
65-69	40.82270514679363
70-74	41.38957557609339
75-79	41.81551529158313
80-84	42.26254390748688
85-89	42.57102958556402
90-94	42.9048649717173
95-99	43.47349123366906
100-101	43.619783081939765
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.0
8	2.0
9	7.0
10	17.5
11	25.5
12	40.5
13	89.5
14	137.5
15	196.5
16	309.0
17	454.0
18	691.5
19	1081.5
20	1623.0
21	2344.0
22	3510.0
23	5149.0
24	7258.0
25	9793.5
26	12962.0
27	16894.5
28	21270.5
29	25843.0
30	31009.5
31	36843.0
32	42661.0
33	48617.0
34	55242.5
35	62970.5
36	71558.5
37	79836.0
38	86441.0
39	89642.5
40	89315.0
41	86892.5
42	83208.0
43	79236.5
44	75810.0
45	71736.5
46	66907.5
47	61081.0
48	53885.0
49	46229.5
50	39195.0
51	33506.5
52	28376.5
53	23911.0
54	19881.5
55	16458.0
56	13759.0
57	11184.5
58	8777.5
59	6877.5
60	5419.5
61	4296.5
62	3346.0
63	2323.5
64	1551.5
65	1138.0
66	841.5
67	593.0
68	443.5
69	460.5
70	472.5
71	386.0
72	333.0
73	275.5
74	222.0
75	170.5
76	100.5
77	54.5
78	30.0
79	16.0
80	9.5
81	4.5
82	3.5
83	3.0
84	1.0
85	0.5
86	1.0
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013004672004026004
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010040816524038683
20-24	6.653553118338885E-4
25-29	0.017444406539353953
30-34	0.0069438899816845825
35-39	0.04198996886137141
40-44	0.05125655374982156
45-49	0.05189771432304332
50-54	0.05141381955080048
55-59	0.04226820835541103
60-64	0.05338569074769
65-69	0.04403442427409735
70-74	0.0039437423937790485
75-79	0.001742021180074181
80-84	1.9355790889713124E-4
85-89	0.003665502899739423
90-94	0.0030364396958237464
95-99	0.002443668599826282
100-101	6.048684653035351E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1653252.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.11943732748567
#Duplication Level	Relative count
1	100.0
2	7.033800970552686
3	2.569499149112732
4	1.847898639181705
5	1.3842703115510202
6	1.0944274400620577
7	0.8875686272151633
8	0.7179925073813719
9	0.5393963811734427
10++	4.191897628940991
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	431740	5.397214	13.034542	75-79
GAGCC	422170	5.2775784	13.124251	75-79
CGAGC	413205	5.165507	12.973929	75-79
CTCCG	454890	5.107055	11.575461	75-79
GTCTC	634870	5.089869	8.755805	60-64
CTGTC	612120	4.9074783	8.554531	55-59
AGCCC	402225	4.5509615	11.658127	80-84
GCCCA	396215	4.482962	11.863312	80-84
TCTCT	809430	4.1941643	6.7495646	60-64
CCACG	360985	4.0843534	11.627406	80-84
TGTCT	708415	4.055712	6.5531263	55-59
TCTCC	556755	4.039919	8.153735	70-74
CCCAC	383935	3.931684	10.641905	80-84
CTCTT	756880	3.9218705	6.412037	60-64
ATCTC	735235	3.8393912	10.253181	95-97
TCCGA	467835	3.7799363	8.61482	75-79
GAGGC	271710	3.7528934	14.363468	95-97
GAGAC	387265	3.484041	9.840299	85-89
CGAGA	385275	3.466138	9.719018	85-89
ACACA	647290	3.4330091	6.3737426	70-74
AGAGG	344390	3.423251	10.837244	90-94
ACGAG	375850	3.3813453	9.435618	95-97
TACAC	606135	3.1898887	7.351265	5
CATCT	603890	3.1535082	6.126196	70-74
CACAT	596040	3.136762	5.8227854	70-74
CACGA	372130	3.030096	8.534396	80-84
AAGAG	467640	3.02771	7.5994587	90-94
ACATC	574265	3.0221672	6.0024347	70-74
CAAGA	480770	2.8172584	6.9855227	90-94
ACAAG	450725	2.6411982	6.60441	85-89
AGAAG	397985	2.5767324	5.50297	5
AGACA	436730	2.559189	6.5794044	85-89
AGGCA	271670	2.444087	9.357098	95-97
GACAA	411700	2.4125159	6.4913154	85-89
GGCAA	266125	2.3942013	9.458884	95-97
TATAC	631860	2.3745682	5.761536	5
CAAAG	331255	1.9411173	5.091432	4
AGAGA	295880	1.915659	5.5312223	8
TCTCG	233610	1.872894	7.3770623	90-94
CTTTG	326590	1.8697445	8.147046	9
GAGAG	187260	1.8613721	6.4340158	7
CAATC	337810	1.7777826	5.8436007	95-97
GCAAT	290980	1.6919305	6.1275187	95-97
CTCGT	201415	1.6147809	7.234778	90-94
GCTTT	271035	1.5516894	5.291331	1
GTATG	234740	1.496407	6.1109433	95-97
TTGAG	227000	1.4470663	5.2389584	9
GTCTT	239355	1.3703197	6.0797553	1
TGCCG	105560	1.3094134	10.846568	95-97
GCCGT	104175	1.2922331	10.231059	95-97
ATGCC	156530	1.2647054	7.3757153	95-97
GAGTC	140255	1.2520549	6.4015603	9
AAGAC	213495	1.251057	5.7733216	5
GATTG	189200	1.2061013	5.9622135	7
GTGTT	187910	1.1886185	5.950547	1
CCGTC	105520	1.1846743	8.40706	95-97
CCATG	145205	1.1732033	5.041769	9
TATGA	280900	1.1663498	5.775908	4
GTTCT	197765	1.1322148	5.024821	1
AGAAC	192935	1.1305777	5.117004	5
GACTT	192725	1.1119555	5.7655406	7
TATGC	190795	1.1008202	5.382616	95-97
TCGTA	188605	1.0881846	5.24687	90-94
TCATA	282335	1.061032	5.16812	2
TGGAC	115245	1.0287908	6.938269	5
GTGTA	160750	1.0247397	9.888704	1
CGTAT	171715	0.9907353	5.268846	95-97
GGACT	104420	0.9321562	6.0985785	6
GTCCA	114655	0.9263706	6.441042	1
AGACT	159190	0.9256252	5.5398064	6
CGTCT	114280	0.9162037	5.4086876	95-97
GAGTA	141510	0.9091169	5.1464205	1
GTATA	168775	0.7007855	7.02692	1
>>END_MODULE