##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-179_AAGAGGCA-TATCCTCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2027320	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74440344888819	33.0	31.0	34.0	30.0	34.0
2	31.94312392715506	33.0	31.0	34.0	30.0	34.0
3	32.090990075567746	34.0	31.0	34.0	30.0	34.0
4	35.628086833849615	37.0	35.0	37.0	33.0	37.0
5	35.49588126196161	37.0	35.0	37.0	33.0	37.0
6	35.633665134265925	37.0	35.0	37.0	33.0	37.0
7	35.64963597261409	37.0	35.0	37.0	33.0	37.0
8	35.704642582325434	37.0	35.0	37.0	33.0	37.0
9	37.523758459444	39.0	38.0	39.0	35.0	39.0
10-14	37.65756851409743	39.2	37.2	39.4	34.8	39.4
15-19	38.7200426178403	40.0	38.2	41.0	35.0	41.0
20-24	38.6246981236312	40.0	38.0	41.0	34.0	41.0
25-29	38.44321774559517	40.0	38.0	41.0	34.0	41.0
30-34	38.18595949332123	40.0	38.0	41.0	33.4	41.0
35-39	37.878399857940536	40.0	38.0	41.0	32.8	41.0
40-44	37.84453732020599	40.0	37.8	41.0	32.8	41.0
45-49	37.70116399976324	40.0	37.2	41.0	32.4	41.0
50-54	36.994333307026025	39.2	36.4	40.2	31.4	40.6
55-59	37.627102184164315	40.0	36.6	41.0	32.6	41.0
60-64	37.19041947003926	39.2	35.6	41.0	31.8	41.0
65-69	36.52470798887201	38.2	35.0	40.4	31.0	41.0
70-74	35.678256022729514	36.6	35.0	39.4	30.4	41.0
75-79	34.672415997474495	35.4	34.0	38.0	29.2	39.6
80-84	33.64613687035101	35.0	33.8	36.4	28.2	38.2
85-89	32.67889400785273	35.0	33.0	35.4	26.0	36.6
90-94	31.850842096955592	35.0	32.2	35.0	24.0	36.0
95-99	31.054220054061517	34.8	31.2	35.0	19.2	35.0
100-101	29.91913536096916	34.0	29.0	35.0	14.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	4.0
6	38.0
7	188.0
8	369.0
9	513.0
10	651.0
11	815.0
12	1159.0
13	1584.0
14	2013.0
15	2407.0
16	2796.0
17	3386.0
18	3944.0
19	4583.0
20	5290.0
21	6041.0
22	7138.0
23	8298.0
24	9943.0
25	11692.0
26	13762.0
27	17015.0
28	20628.0
29	25318.0
30	31576.0
31	40566.0
32	53763.0
33	73048.0
34	103583.0
35	157654.0
36	264080.0
37	472339.0
38	565935.0
39	115153.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.405222275841716	23.40106166604001	10.940055047157196	24.253661010961075
2	15.065283916699478	23.94421237428985	39.76940680500087	21.221096904009798
3	15.78028085344475	27.843627559576877	33.04604265644867	23.3300489305297
4	11.348799849219478	18.500809409178174	44.34353486577524	25.80685587582711
5	11.027152543025203	39.226441916038254	38.698578597999365	11.047826942937176
6	25.233256234841118	43.827261888730554	19.645366666715994	11.294115209712338
7	23.45147563188693	34.20059478094274	26.876571362614627	15.471358224555704
8	21.230012312257802	38.01557579536396	24.577654041340775	16.176757851037465
9	23.080254504361193	14.907367535254792	25.06810739140922	36.944270568974794
10-14	20.45171774310428	25.93910133370666	32.9556949480593	20.653485975129755
15-19	19.82902066239618	31.50845448489458	28.768328888486295	19.894195964222952
20-24	19.823037944657322	30.007374734597374	30.253403143014395	19.916184177730905
25-29	19.74739345226844	30.353155105621365	29.919865009220242	19.979586432889953
30-34	19.662493753949256	30.267042408033223	29.939164822242205	20.131299015775316
35-39	19.746715430005636	30.404990908669372	29.942936238349727	19.905357422975268
40-44	19.54115994135372	29.953627604007366	30.44336943802576	20.061843016613157
45-49	19.485921844745157	29.619819857861867	30.67203353192366	20.222224765469317
50-54	18.986763692472525	29.31616439599512	31.107557269116874	20.589514642415477
55-59	18.939304700619697	29.256534820906925	31.147029104880925	20.657131373592453
60-64	18.717387784676575	28.814273202493272	31.22730704830833	21.241031964521817
65-69	19.143423087214632	28.706037687000407	30.499852396659904	21.65068682912506
70-74	19.40216126304859	28.5565817229502	30.0503398797838	21.990917134217412
75-79	20.112899771140047	28.979893532814522	29.239693039379954	21.667513656665477
80-84	20.61293335916969	29.03241528759851	28.869277243708012	21.48537410952379
85-89	21.01753298626681	29.090159453478257	28.691187846969097	21.201119713285838
90-94	21.527174711983672	28.661179137919294	28.79941316395201	21.012232986145026
95-99	22.312850818849043	28.235224010534594	28.316184957582323	21.13574021303404
100-101	23.07971205731624	27.50722791779239	28.37258023223062	21.040479792660747
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.65888328680279
2	36.286380820709276
3	39.11032978397445
4	37.155655725046586
5	22.07497948596238
6	36.52737144455346
7	38.92283385644263
8	37.40677016329526
9	60.02452507333599
10-14	41.10520371823404
15-19	39.723216626619134
20-24	39.73922212238823
25-29	39.72697988515839
30-34	39.793792769724575
35-39	39.6520728529809
40-44	39.60300295796688
45-49	39.70814661021447
50-54	39.576278334888
55-59	39.59643607421215
60-64	39.95841974919839
65-69	40.794109916339686
70-74	41.393078397266
75-79	41.78041342780553
80-84	42.09830746869348
85-89	42.21865269955264
90-94	42.5394076981287
95-99	43.44859103188308
100-101	44.12019184997698
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.5
2	3.0
3	8.0
4	12.5
5	14.0
6	18.0
7	23.5
8	35.5
9	47.5
10	55.5
11	69.5
12	107.5
13	157.5
14	204.5
15	292.5
16	418.0
17	578.0
18	847.5
19	1238.5
20	1795.0
21	2637.5
22	3878.0
23	5640.0
24	7970.5
25	11040.5
26	15103.0
27	19797.0
28	24938.0
29	30839.5
30	37434.5
31	44297.5
32	51658.0
33	59603.5
34	68203.0
35	77865.5
36	88874.0
37	99217.0
38	106086.0
39	109165.5
40	109144.0
41	106558.5
42	102077.0
43	97271.0
44	92534.5
45	86345.0
46	79081.0
47	71631.5
48	63249.0
49	54450.0
50	46532.0
51	40401.0
52	34896.5
53	29508.5
54	25042.0
55	21089.5
56	17862.5
57	14977.5
58	12115.0
59	10081.5
60	8226.5
61	6594.5
62	5410.0
63	4001.0
64	2948.0
65	2476.5
66	2033.5
67	1585.5
68	1360.0
69	1301.0
70	1227.5
71	1051.5
72	891.5
73	743.5
74	588.0
75	468.0
76	344.5
77	245.0
78	184.5
79	139.0
80	101.0
81	67.0
82	57.0
83	48.0
84	38.5
85	26.5
86	21.0
87	19.5
88	13.0
89	13.5
90	13.0
91	10.5
92	8.0
93	5.0
94	7.5
95	11.0
96	7.5
97	3.0
98	2.0
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034232385612532806
2	0.03127281336937435
3	0.028066610105952686
4	0.026290866760057614
5	0.03250596847069037
6	0.0021210267742635598
7	9.371978770001776E-4
8	0.0035514866917901464
9	3.452834283684865E-4
10-14	0.007033916697906596
15-19	0.023607521259593946
20-24	0.019710751139435313
25-29	0.0020125091253477495
30-34	0.0019237219580529959
35-39	0.005623187262001065
40-44	0.012430203421265513
45-49	0.008148688909496282
50-54	0.004587336976895606
55-59	0.003571217173411203
60-64	0.010052680385928221
65-69	0.013101039796381431
70-74	0.01670185269222422
75-79	0.006974725253043426
80-84	0.009983623700254523
85-89	0.007803405481127794
90-94	0.008780064321370084
95-99	0.00837558944813843
100-101	0.0054752086498431425
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2027320.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	22.31707934113145
#Duplication Level	Relative count
1	100.0
2	7.137401190158984
3	2.2642626639429198
4	1.4962844539183469
5	1.1072623382775262
6	0.8846256328270716
7	0.694555466737721
8	0.5725789738579506
9	0.477247831364538
10++	3.7895609438375226
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	754745	4.951894	8.22797	60-64
GCTGC	497115	4.861105	11.85446	90-94
CTGTC	723360	4.745976	8.127379	55-59
GACGC	470225	4.7124696	11.840311	75-79
CGCTG	466550	4.562221	11.768413	75-79
CTGCC	452140	4.282814	11.428911	90-94
TCTCT	970070	4.2703857	6.667922	60-64
GCCGA	425615	4.2654	12.088325	80-84
CGACG	416595	4.1750035	12.20241	95-97
TGCCG	420280	4.1097636	11.586981	90-94
CCGAC	409340	3.9737923	11.526945	80-84
CTCTT	899990	3.9618835	6.308394	60-64
TGTCT	838645	3.8112202	6.091747	65-69
GAAGA	748980	3.7824404	7.9110336	85-89
ACACA	764445	3.6224673	6.6688285	6
CTGAC	527455	3.5466735	7.960355	85-89
TGACG	508310	3.5284693	8.384084	85-89
GAGGA	461500	3.3893464	9.386225	90-94
TACAC	712055	3.2923574	7.823556	5
ATCTG	703420	3.2761617	6.543204	70-74
CACAT	704050	3.255345	5.8916373	65-69
CATCT	713410	3.2186067	6.1128573	70-74
GACGA	447670	3.1847868	8.927835	95-97
ACGCT	473390	3.1831338	8.092367	75-79
ACATC	680990	3.1487215	6.0239754	70-74
AGAGG	426215	3.130206	9.257284	90-94
TCTGA	659340	3.07086	6.085997	70-74
CGAAG	414560	2.9492376	8.80505	85-89
AAGAG	570395	2.8805647	6.716363	90-94
TATAC	740345	2.3710592	5.378604	5
ACGAA	458305	2.241995	6.1381702	85-89
AGGAT	425360	2.096012	6.1801467	90-94
CAAAG	405950	1.9858782	5.2587686	4
AGAGA	365145	1.8440268	5.091754	8
CTTTG	405320	1.8419758	7.9824014	9
GGATA	372330	1.8347007	5.920132	90-94
GGTGG	169195	1.7632315	6.845715	95-97
GAGAG	236990	1.7405008	5.68432	7
GATAG	339125	1.671079	5.681967	95-97
TAGTG	342460	1.6465781	5.3435783	95-97
GCTTT	338465	1.5381534	5.178541	1
GTGTA	315925	1.5189954	9.136179	1
CTCGG	151705	1.4834675	6.8519974	95-97
AAGAC	260760	1.2756191	5.7476254	5
CGGTG	119880	1.210171	5.873974	95-97
GAGTC	172205	1.1953729	5.9382095	9
GTCTT	258145	1.1731393	6.1463485	1
GTGTT	242365	1.137045	5.7875214	1
AACAC	238745	1.1313384	5.05496	5
GACTT	240145	1.1184696	5.628596	7
GATTG	230220	1.1069182	5.3605933	7
TATGA	334505	1.1059428	5.481967	4
TCATA	334435	1.0710753	5.4934907	2
TGGAC	139685	0.96963316	6.1522	5
AGACT	196990	0.9402849	5.2313967	6
GTCCA	138455	0.9309887	6.8174286	1
GGACT	127850	0.88747954	5.7462573	6
GAGTA	175140	0.86302334	5.089222	1
GTATA	200905	0.6642337	6.7603045	1
>>END_MODULE