##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-179_AAGAGGCA-TATCCTCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2027320	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.452939841761534	34.0	31.0	34.0	31.0	34.0
2	32.638185880867354	34.0	31.0	34.0	31.0	34.0
3	32.74777045557682	34.0	31.0	34.0	31.0	34.0
4	36.26564972475978	37.0	37.0	37.0	35.0	37.0
5	35.991915435155775	37.0	35.0	37.0	35.0	37.0
6	36.07643440601385	37.0	36.0	37.0	35.0	37.0
7	36.07470552256181	37.0	36.0	37.0	35.0	37.0
8	36.098691869068524	37.0	36.0	37.0	35.0	37.0
9	37.97481058737644	39.0	38.0	39.0	35.0	39.0
10-14	38.1611916224375	39.4	38.2	39.4	35.2	39.4
15-19	39.28973916303297	40.4	39.0	41.0	36.0	41.0
20-24	39.22331294516899	40.0	39.0	41.0	36.0	41.0
25-29	39.031773671645325	40.0	39.0	41.0	36.0	41.0
30-34	38.83163851784623	40.0	38.0	41.0	35.0	41.0
35-39	38.60653345303159	40.0	38.0	41.0	34.6	41.0
40-44	38.36842313990884	40.0	38.0	41.0	34.0	41.0
45-49	38.1966760057613	40.0	38.0	41.0	33.4	41.0
50-54	38.154011305565966	40.0	37.4	41.0	33.4	41.0
55-59	37.78612739971983	39.8	36.6	41.0	33.0	41.0
60-64	37.23643697097647	39.0	35.6	40.8	32.4	41.0
65-69	36.496383895980905	37.6	35.0	40.0	31.0	41.0
70-74	35.90034942682951	36.6	35.0	39.2	31.0	41.0
75-79	34.664949983229086	35.2	33.8	37.4	30.2	39.2
80-84	34.42571167847208	35.0	34.0	36.4	31.0	38.2
85-89	33.7799281810469	35.0	34.0	35.4	30.0	36.6
90-94	33.35984807529152	35.0	34.0	35.0	29.4	36.0
95-99	33.07373389499437	35.0	33.4	35.0	29.0	35.2
100-101	32.71548670165539	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	5.0
14	27.0
15	75.0
16	204.0
17	377.0
18	683.0
19	1094.0
20	1614.0
21	2356.0
22	3280.0
23	4632.0
24	6151.0
25	8049.0
26	10222.0
27	13684.0
28	16962.0
29	21402.0
30	26946.0
31	34176.0
32	43819.0
33	59043.0
34	84894.0
35	134420.0
36	251099.0
37	495833.0
38	666449.0
39	139739.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.959789278456284	23.42170944892765	11.226298758952707	24.392202513663356
2	15.213528310766744	23.870938778651414	39.52486337207294	21.390669538508895
3	15.880127458911272	27.579119231300435	33.27111654795494	23.269636761833357
4	11.48037803602786	18.53338397490283	44.03508079632224	25.951157192747075
5	10.865230945287374	39.35328413866582	38.74277371110629	11.038711204940514
6	25.233559576189258	44.10902077619714	19.44004893159442	11.217370716019177
7	23.44523804826076	34.16929739754948	26.849436694749716	15.536027859440049
8	21.102835270208946	38.237870686423456	24.42021979756526	16.23907424580234
9	22.92716492709587	14.81251109839591	25.06037527376043	37.19994870074779
10-14	20.379229721997515	25.9672967267131	32.914951758972435	20.73852179231695
15-19	19.74822086078404	31.569003629752785	28.735452453375686	19.947323056087487
20-24	19.73688442222953	30.08725665005415	30.243651330918436	19.93220759679788
25-29	19.737596401827265	30.43834969820192	29.824738661037546	19.999315238933267
30-34	19.639772997911788	30.31322594387379	29.877913666041106	20.169087392173317
35-39	19.735611830653088	30.444826887194765	29.8905623336175	19.928998948534648
40-44	19.518838315631097	29.987610260831993	30.368659141624892	20.124892281912018
45-49	19.46393076576793	29.619352074483473	30.631260085250812	20.285457074497785
50-54	18.953256656576233	29.358055056846275	31.044029092818487	20.644659193759
55-59	18.82779729786544	29.26409083094516	31.043805024227783	20.864306846961615
60-64	18.411290021700495	28.8009869352872	31.023219904997905	21.764503138014398
65-69	18.698373126786365	28.846957064127466	30.086594185734363	22.36807562335181
70-74	18.663575466393063	28.95464814577311	29.47588370509949	22.90589268273433
75-79	19.22341611789744	29.478306601972225	28.47210829094268	22.826168989187657
80-84	19.28890182334763	29.69143916954198	27.86809153979525	23.151567467315143
85-89	19.441158959328	29.650647891964898	27.60927889616908	23.298914252538022
90-94	19.490121015756536	29.107068549024373	27.853335209087753	23.549475226131335
95-99	19.67535649452257	28.61383580440848	27.78306838584189	23.927739315227058
100-101	19.573352998046683	27.75341337332044	28.5566906063177	24.116543022315174
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.35199179211965
2	36.60419784927564
3	39.14976422074463
4	37.43153522877493
5	21.903942150227888
6	36.45093029220843
7	38.98126590770081
8	37.341909516011285
9	60.127113627843656
10-14	41.11775151431446
15-19	39.69554391687153
20-24	39.66909201902741
25-29	39.73691164076053
30-34	39.8088603900851
35-39	39.66461077918774
40-44	39.64373059754311
45-49	39.74938784026571
50-54	39.597915850335234
55-59	39.692104144827056
60-64	40.17579315971489
65-69	41.06644875013817
70-74	41.569468149127395
75-79	42.04958510708509
80-84	42.440469290662776
85-89	42.740073211866026
90-94	43.03959624188787
95-99	43.603095809749625
100-101	43.68989602036186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	1.0
5	0.0
6	0.5
7	1.5
8	1.5
9	6.0
10	15.0
11	34.0
12	50.0
13	83.0
14	158.5
15	232.5
16	338.0
17	521.5
18	763.0
19	1169.5
20	1763.5
21	2575.0
22	3834.5
23	5649.5
24	8081.5
25	11205.5
26	15156.5
27	19522.0
28	24744.0
29	30684.5
30	36909.5
31	43704.5
32	50733.5
33	58396.0
34	66900.0
35	76524.0
36	87210.5
37	97260.5
38	104480.0
39	107634.0
40	107387.5
41	104381.0
42	100017.5
43	95689.5
44	92042.5
45	87366.0
46	81336.0
47	74942.0
48	66635.5
49	57405.0
50	49113.0
51	42377.0
52	36394.5
53	30869.0
54	25991.0
55	21680.0
56	18382.0
57	15285.0
58	12062.5
59	9878.5
60	8281.0
61	6847.5
62	5475.0
63	3882.0
64	2853.5
65	2403.0
66	1979.5
67	1560.0
68	1298.5
69	1257.5
70	1191.5
71	1011.0
72	897.0
73	762.5
74	572.0
75	442.0
76	333.5
77	225.0
78	150.0
79	108.0
80	65.0
81	45.5
82	34.5
83	22.0
84	16.0
85	9.0
86	5.0
87	4.5
88	3.0
89	2.0
90	1.0
91	0.0
92	0.0
93	1.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01390998954284474
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	9.56928358621234E-4
20-24	6.511058934948603E-4
25-29	0.016553874080066294
30-34	0.006491328453327546
35-39	0.04019099106209182
40-44	0.047639247874040606
45-49	0.04783655269025117
50-54	0.04824102756348282
55-59	0.03963853757670225
60-64	0.049661622240198884
65-69	0.042223230669060634
70-74	0.0029595722431584558
75-79	0.0012726160645581358
80-84	2.466310202632046E-4
85-89	0.002614288814789969
90-94	0.0023084663496635953
95-99	0.0018645305131898269
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2027320.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	22.810825196387928
#Duplication Level	Relative count
1	100.0
2	7.262978308554716
3	2.3403458504485117
4	1.5045505073622514
5	1.149664433519792
6	0.9071292272756996
7	0.7145277399642141
8	0.5742377677249841
9	0.505281679675193
10++	3.931091467873002
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	516535	5.1005235	12.0673	75-79
GAGCC	496250	4.9002194	12.054852	75-79
GTCTC	752830	4.8606267	8.100113	60-64
CTCCG	538810	4.8035593	10.624851	85-89
CGAGC	486190	4.8008823	11.91026	75-79
CTGTC	723830	4.673389	7.978107	55-59
AGCCC	471235	4.230601	10.639269	90-94
GCCCA	466270	4.1860275	10.802951	90-94
TCTCT	969270	4.120572	6.406086	60-64
TGTCT	839235	3.924161	6.2720942	65-69
TCTCC	662460	3.8887007	7.707244	70-74
CTCTT	899580	3.8243053	6.1374125	60-64
CCACG	422745	3.7952733	10.724143	80-84
ATCTC	861130	3.6865363	9.787437	95-97
CCCAC	450755	3.679211	9.811117	80-84
TCCGA	556865	3.6206148	8.0399885	85-89
GAGGC	320870	3.4849346	13.056389	90-94
ACACA	768710	3.337232	6.1253085	6
GAGAC	457375	3.2937634	9.105621	95-97
CGAGA	455045	3.276984	9.010644	85-89
AGAGG	406200	3.2174392	10.042802	90-94
ACGAG	441245	3.177604	8.860026	95-97
TACAC	715890	3.0862644	7.474729	5
CATCT	714000	3.0566664	5.796075	70-74
CACAT	707940	3.0519915	5.5527506	80-84
ACATC	685530	2.95538	5.64778	70-74
AAGAG	554425	2.9118412	7.2870116	90-94
CACGA	436015	2.8547685	7.9499855	80-84
CAAGA	571880	2.7307334	6.6460385	90-94
ACAAG	540990	2.5832334	6.3534164	85-89
AGAAG	488045	2.563213	5.1973143	5
AGACA	511805	2.4438746	6.2043056	85-89
GACAA	491280	2.3458672	6.1806884	85-89
TATAC	741815	2.3160627	5.367902	5
AGGCA	320265	2.3063722	8.616759	95-97
GGCAA	317655	2.2875767	8.763418	95-97
CAAAG	408890	1.9524543	5.28325	4
AGAGA	364035	1.9119124	5.546179	8
CTTTG	402405	1.8815969	8.052807	9
GAGAG	233390	1.8486414	6.432745	7
TCTCG	277195	1.7897019	6.840444	95-97
CAATC	402090	1.7334453	5.621021	95-97
GCAAT	343475	1.6286676	5.918115	95-97
GCTTT	336940	1.5754905	5.2014384	1
CTCGT	239710	1.5476811	6.573296	95-97
TTGAG	283175	1.4665797	5.3511276	9
GTATG	279055	1.4452419	5.7105603	95-97
GTCTT	296530	1.3865383	6.196831	1
TGCCG	128510	1.2601287	9.409388	95-97
GCCGT	127810	1.2532648	8.995735	95-97
GAGTC	173835	1.2431401	6.095549	9
GTGTT	241670	1.2429007	6.177411	1
AAGAC	260170	1.2423147	5.7810144	5
ATGCC	189335	1.231015	6.6688166	95-97
GATTG	228305	1.1824048	5.84079	7
CCATG	178790	1.1624537	5.0943046	9
CCGTC	129795	1.1571387	7.5043783	95-97
TATGA	335645	1.1526178	5.742612	4
GACTT	239705	1.128697	5.538628	7
GTTCT	240130	1.1228187	5.2694607	1
TCATA	335320	1.0469215	5.2792645	2
TATGC	222080	1.0457063	5.030579	95-97
GTGTA	197190	1.0212585	9.854772	1
TGGAC	137355	0.982262	6.8063498	5
AGACT	196285	0.9307316	5.1705637	6
GGACT	126990	0.9081392	6.067811	6
CGTCT	140390	0.9064243	5.077895	95-97
GAGTA	173490	0.90482	5.035306	1
GTCCA	138720	0.90192723	6.192139	1
GTATA	199655	0.685623	6.722499	1
>>END_MODULE