##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-178_AAGAGGCA-CTCTCTAT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1430012	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262171925829993	31.0	31.0	34.0	28.0	34.0
2	31.453067526706068	31.0	31.0	34.0	28.0	34.0
3	31.586416757341897	33.0	31.0	34.0	28.0	34.0
4	35.124345809685515	37.0	35.0	37.0	32.0	37.0
5	34.990888188350866	37.0	35.0	37.0	32.0	37.0
6	35.155337158009864	37.0	35.0	37.0	32.0	37.0
7	35.157568607815875	37.0	35.0	37.0	32.0	37.0
8	35.2047752046836	37.0	35.0	37.0	32.0	37.0
9	36.935365577351796	39.0	37.0	39.0	33.0	39.0
10-14	37.01628252070613	39.2	37.2	39.4	32.2	39.4
15-19	37.98487956744418	40.0	38.0	41.0	32.6	41.0
20-24	37.85660316137207	40.0	38.0	41.0	32.0	41.0
25-29	37.64143881310087	40.0	37.6	41.0	31.8	41.0
30-34	37.35024671121641	40.0	37.0	41.0	30.8	41.0
35-39	37.00724329586045	39.8	36.4	41.0	30.0	41.0
40-44	36.941364408130845	39.6	36.0	41.0	30.0	41.0
45-49	36.74546213598208	39.2	36.0	41.0	29.8	41.0
50-54	36.139854630590506	38.6	35.2	39.8	29.2	40.6
55-59	36.78524306089739	39.0	35.6	41.0	29.8	41.0
60-64	36.31192003983183	38.8	35.0	40.6	29.0	41.0
65-69	35.61590056586938	37.4	34.4	40.0	28.2	41.0
70-74	34.77998786024173	36.2	34.0	39.0	27.6	40.8
75-79	33.83778737521084	35.2	33.2	37.6	26.2	39.4
80-84	32.94221349191476	35.0	32.6	36.2	25.8	38.0
85-89	32.185571170032134	35.0	32.0	35.2	24.8	36.6
90-94	31.61631273024282	34.4	31.2	35.0	23.6	36.0
95-99	31.138993239217577	34.0	31.0	35.0	21.0	35.0
100-101	30.287854927091523	34.0	29.5	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	9.0
6	41.0
7	159.0
8	302.0
9	420.0
10	573.0
11	903.0
12	1540.0
13	2463.0
14	3046.0
15	3577.0
16	4075.0
17	4594.0
18	5088.0
19	5614.0
20	6259.0
21	7067.0
22	7954.0
23	8893.0
24	10227.0
25	11829.0
26	13595.0
27	15819.0
28	18645.0
29	22134.0
30	26656.0
31	33301.0
32	43086.0
33	56642.0
34	79238.0
35	117726.0
36	194881.0
37	325414.0
38	338201.0
39	59988.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.084583142131244	23.36969903857992	10.227260008730008	24.318457810558833
2	15.011017144775776	23.414475478983483	40.315125315649944	21.2593820605908
3	15.798320268208693	27.909190680405395	32.66894370793064	23.62354534345528
4	10.920410955454644	18.101919079155373	44.54213776296756	26.435532202422422
5	10.553743832095904	39.626729006741854	39.01822775672261	10.801299404439632
6	25.83612238932471	44.18116439649397	19.118262034665882	10.864451179515438
7	23.79905762369563	34.09792433856736	26.708354254049993	15.394663783687015
8	21.236438450811978	38.8185839903438	23.929167348502965	16.015810210341254
9	22.815854434584374	14.6774498080433	25.237795539891327	37.268900217481
10-14	20.354445598823318	25.954320724494895	33.00580397080223	20.685429705879553
15-19	19.64934967918327	31.823066629100722	28.71513270674304	19.81245098497297
20-24	19.62962253163172	30.23718353764143	30.362942445086095	19.770251485640753
25-29	19.617315890895533	30.65100886471705	29.88925187673481	19.84242336765261
30-34	19.53031336312203	30.464721543581767	29.986058577471397	20.018906515824803
35-39	19.6510995416426	30.671104546075682	29.92189419889978	19.755901713381935
40-44	19.389727653803202	30.487530241416582	30.290373550034577	19.832368554745635
45-49	19.574125908946716	30.399942149671222	30.177520503577597	19.84841143780446
50-54	19.36195471929499	30.066763528377088	30.560685521318902	20.010596231009025
55-59	19.589947591313788	30.036662722949508	30.591799539257842	19.78159014647886
60-64	19.263904080656395	29.76933535123522	30.838888624684234	20.127871943424147
65-69	19.59429905390346	29.94202453777483	30.346301520242037	20.117374888079674
70-74	19.519205724522063	29.832457516372784	30.41394346483317	20.234393294271978
75-79	19.78170285813553	30.170077128243978	30.069063368021435	19.979156645599055
80-84	19.833233043205322	30.246341648322105	29.828172378168478	20.092252930304095
85-89	20.020611485252214	30.217128345659756	29.689224792192704	20.073035376895334
90-94	19.942657667984317	29.954892358640507	30.122124508503788	19.980325464871388
95-99	20.29385583820417	29.923338549653984	29.644082627861867	20.138722984279983
100-101	20.39846956580119	29.295499541583066	30.246950705668358	20.05908018694739
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.40304095269008
2	36.27039920536657
3	39.42186561166397
4	37.355943157877064
5	21.355043236535536
6	36.70057356884015
7	39.19372140738265
8	37.25224866115323
9	60.08475465206538
10-14	41.039875304702875
15-19	39.461800664156236
20-24	39.399874017272474
25-29	39.45973925854814
30-34	39.54921987894683
35-39	39.40700125502454
40-44	39.222096208548834
45-49	39.42253734675118
50-54	39.37255095030402
55-59	39.37153773779265
60-64	39.39177602408054
65-69	39.711673941983136
70-74	39.75359901879404
75-79	39.760859503734586
80-84	39.92548597350942
85-89	40.093646862147544
90-94	39.922983132855705
95-99	40.43257882248415
100-101	40.45754975274858
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	3.5
4	10.0
5	14.0
6	18.5
7	28.5
8	29.0
9	31.0
10	47.5
11	72.0
12	97.0
13	144.0
14	224.5
15	325.0
16	462.5
17	659.0
18	931.5
19	1334.5
20	1898.0
21	2786.0
22	3952.0
23	5476.5
24	7416.0
25	9767.0
26	12722.0
27	16275.5
28	20355.0
29	24737.0
30	29759.5
31	35138.5
32	40533.5
33	46632.5
34	53932.0
35	61627.0
36	70164.5
37	77775.5
38	82040.5
39	82761.0
40	79948.0
41	75311.5
42	69677.0
43	63890.5
44	58555.0
45	52978.5
46	47827.0
47	42716.0
48	37130.5
49	31646.0
50	26953.5
51	23591.0
52	20588.0
53	18030.0
54	15648.0
55	13616.0
56	12129.0
57	10213.0
58	8349.0
59	6998.5
60	5612.0
61	4470.0
62	3528.5
63	2470.5
64	1655.5
65	1209.5
66	918.0
67	666.5
68	512.5
69	529.0
70	541.5
71	440.5
72	352.5
73	290.0
74	223.5
75	146.0
76	103.0
77	78.0
78	50.5
79	35.0
80	29.0
81	25.5
82	21.5
83	16.5
84	17.0
85	12.0
86	8.5
87	11.0
88	8.5
89	7.5
90	6.5
91	3.5
92	3.0
93	4.0
94	5.0
95	5.0
96	4.0
97	3.5
98	3.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03216756223024702
2	0.029510241872096176
3	0.02650327409839917
4	0.025734049784197615
5	0.030349395669406972
6	0.0012587306959661877
7	6.992948310923265E-4
8	0.003496474155461632
9	1.398589662184653E-4
10-14	0.006601343205511563
15-19	0.024363431915256657
20-24	0.02043339496451778
25-29	0.0019859973203022073
30-34	0.0020139691135459005
35-39	0.005580372752116765
40-44	0.012922968478586193
45-49	0.008055876454183602
50-54	0.004867092024402593
55-59	0.00406989591695734
60-64	0.010069845567729501
65-69	0.013999882518468378
70-74	0.018265580988131566
75-79	0.00753839827917528
80-84	0.011216689090720917
85-89	0.009062861010956552
90-94	0.010027887877863963
95-99	0.009846071221779957
100-101	0.006363582962940172
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1430012.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.396060263120663
#Duplication Level	Relative count
1	100.0
2	7.0169011039171645
3	2.695527874208699
4	1.7922137202849284
5	1.351367583995003
6	1.1027159485399225
7	0.8744849787985441
8	0.7249336328364304
9	0.5621689149419212
10++	4.001249264255427
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CTCTT	351910	2.2983491	5.1798773	1
GAGAG	215825	2.2277563	5.931733	7
CAAAG	315595	2.1245577	5.1006927	4
CTTTG	307700	2.0377316	8.208792	9
ACACA	298475	1.9815782	7.48861	6
GCTTT	261735	1.7333302	5.139926	1
CACAT	255890	1.689599	5.1841135	7
TACAC	252145	1.6648712	9.426062	5
TGGCT	153295	1.5435219	5.029331	6
TTGAG	216555	1.4621633	5.3634033	9
ATACA	323365	1.4317466	5.0372024	6
GACTC	140780	1.4056058	5.12762	7
CCATG	138970	1.3875341	5.786146	9
GAGTC	133870	1.3553175	6.27953	9
AAGAC	198965	1.3394148	5.7929764	5
GTCTT	196825	1.3034663	6.678692	1
GACTT	184325	1.2273735	6.167699	7
AGAAC	181470	1.22164	5.0125246	5
GTGTA	179630	1.2128484	10.467712	1
TATGA	271570	1.2125989	5.787374	4
GTTCT	183015	1.21201	5.3230357	1
TCATA	274795	1.210066	5.95734	2
GATTG	179185	1.2098439	5.956042	7
GTGTT	178510	1.1987185	5.840531	1
ACCAT	175230	1.1570145	5.011308	8
TATAC	261900	1.1532825	6.1048098	5
ACATG	167650	1.1224554	5.2665086	8
TGGAC	110120	1.1148694	7.410657	5
GTCCA	109735	1.0956398	7.526463	1
ACACT	153465	1.0133038	5.1808033	6
AGACT	148730	0.9957816	5.5649924	6
GGACT	97705	0.98917824	6.7948847	6
GAGTA	141065	0.9576789	5.3679147	1
GTATG	139765	0.9436829	5.272938	3
CATAC	138355	0.9135349	5.1308804	3
GTATA	166430	0.7431337	7.3556385	1
CCGTG	49150	0.74205893	5.0153856	9
>>END_MODULE