##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-178_AAGAGGCA-CTCTCTAT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1430012	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32207282176653	33.0	31.0	34.0	31.0	34.0
2	32.50843139777848	34.0	31.0	34.0	31.0	34.0
3	32.613033317202934	34.0	31.0	34.0	31.0	34.0
4	36.15750567128108	37.0	35.0	37.0	35.0	37.0
5	35.86050047132471	37.0	35.0	37.0	35.0	37.0
6	35.97500860132642	37.0	35.0	37.0	35.0	37.0
7	35.97412818913408	37.0	35.0	37.0	35.0	37.0
8	35.99828462977933	37.0	35.0	37.0	35.0	37.0
9	37.82227841444687	39.0	38.0	39.0	35.0	39.0
10-14	37.98182071199402	39.2	37.8	39.4	35.2	39.4
15-19	39.08017051605161	40.0	38.2	41.0	36.0	41.0
20-24	39.00282780843797	40.0	38.8	41.0	36.0	41.0
25-29	38.78813212756257	40.0	38.0	41.0	35.0	41.0
30-34	38.57984380550653	40.0	38.0	41.0	34.4	41.0
35-39	38.334797190513086	40.0	38.0	41.0	34.0	41.0
40-44	38.091820208501744	40.0	38.0	41.0	33.2	41.0
45-49	37.9047122681488	40.0	37.0	41.0	33.0	41.0
50-54	37.84348285189215	40.0	37.0	41.0	33.0	41.0
55-59	37.44551598168407	39.2	36.2	41.0	32.2	41.0
60-64	36.87207226233066	38.8	35.2	40.2	31.2	41.0
65-69	36.11171430729253	37.6	34.8	40.0	30.6	41.0
70-74	35.54910434318034	36.4	34.2	39.0	30.0	40.8
75-79	34.437157450426994	35.2	33.4	37.4	29.4	39.2
80-84	34.288596039753514	35.0	34.0	36.4	30.0	38.2
85-89	33.65821349750911	35.0	34.0	35.6	29.6	36.8
90-94	33.228631507987345	35.0	33.8	35.0	29.0	36.0
95-99	32.93945868985715	35.0	33.0	35.0	29.0	35.4
100-101	32.58030771769748	34.5	32.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	13.0
15	55.0
16	130.0
17	270.0
18	423.0
19	780.0
20	1135.0
21	1673.0
22	2408.0
23	3358.0
24	4815.0
25	6114.0
26	8080.0
27	10256.0
28	13473.0
29	17158.0
30	21908.0
31	27762.0
32	35757.0
33	48893.0
34	69487.0
35	109980.0
36	196461.0
37	359696.0
38	413871.0
39	75992.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.269793540194065	23.472530300445033	10.82368539564703	24.43399076371387
2	15.333889104141285	23.291179609552355	40.06914031301273	21.30579097329363
3	16.02154387515629	27.418161525917267	33.1262954436746	23.433999155251843
4	11.217807962450664	18.143554040105958	44.134175097831346	26.50446289961203
5	10.404667932856508	39.65589099951609	39.15617491321751	10.783266154409892
6	25.735168655927364	44.495221019124315	18.95704371711566	10.812566607832661
7	23.626235304319124	34.01572853934093	26.810264529248705	15.547771627091242
8	21.20352836199976	38.86799551332437	23.909589569877735	16.018886554798144
9	22.716732447000446	14.45617239575612	25.16671188773241	37.66038326951103
10-14	20.258403425985236	25.882160429422967	33.04306537287799	20.816370771713803
15-19	19.55473970188707	31.865356172574028	28.718198315378725	19.861705810160174
20-24	19.579485549938152	30.22732574695063	30.3456473590784	19.847541344032816
25-29	19.536204337434068	30.67390546856214	29.912079946497922	19.877810247505867
30-34	19.465654727303956	30.381315133269517	30.06655503294463	20.0864751064819
35-39	19.62452572163487	30.650841857215173	29.94396106768913	19.78067135346082
40-44	19.30018787794748	30.459021352768794	30.36292053030079	19.87787023898293
45-49	19.458400935267957	30.393633909200368	30.21245722625327	19.93550792927841
50-54	19.279544510039052	30.005904914091897	30.619470264839592	20.095080311029456
55-59	19.50220217905124	29.988950978132955	30.633745422533092	19.87510142028271
60-64	19.12658273192039	29.77171775178465	30.809204052778817	20.292495463516147
65-69	19.404024833860976	29.968069910679418	30.302117885145424	20.32578737031418
70-74	19.300819369129268	29.86862932215013	30.307342796669374	20.523208512051227
75-79	19.609014408670518	30.191955698593453	29.877994626801296	20.321035265934732
80-84	19.54401348468735	30.299833455384	29.592144710879776	20.56400834904887
85-89	19.593353205240096	30.238194341877357	29.531362590687955	20.637089862194593
90-94	19.43183072183004	29.948749909263718	29.94084763563274	20.67857173327351
95-99	19.599479656985363	29.92509404678304	29.566977099956237	20.90844919627536
100-101	19.419431039341656	29.252432323947158	30.370046104540453	20.958090532170733
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.70378430390794
2	36.63968007743491
3	39.45554303040814
4	37.72227086206269
5	21.1879340872664
6	36.547735263760025
7	39.17400693141037
8	37.2224149167979
9	60.37711571651146
10-14	41.074774197699035
15-19	39.41644551204725
20-24	39.42702689397097
25-29	39.414014584939935
30-34	39.552129833785855
35-39	39.40519707509569
40-44	39.17805811693041
45-49	39.39390886454636
50-54	39.37462482106851
55-59	39.37730359933395
60-64	39.41907819543653
65-69	39.72981220417516
70-74	39.8240278811805
75-79	39.93004967460525
80-84	40.108021833736224
85-89	40.23044306743469
90-94	40.11040245510355
95-99	40.50792885326072
100-101	40.37752157151239
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	4.5
7	10.0
8	12.0
9	14.0
10	23.0
11	47.0
12	81.0
13	128.5
14	184.0
15	269.5
16	418.0
17	656.5
18	975.5
19	1383.0
20	2024.0
21	2897.0
22	4102.0
23	5789.0
24	7715.5
25	10111.0
26	13060.5
27	16323.0
28	20318.0
29	24644.5
30	29457.0
31	35103.0
32	40446.0
33	46296.5
34	53061.0
35	60364.0
36	69256.5
37	77239.5
38	81843.5
39	82474.0
40	79369.5
41	74606.5
42	68740.5
43	63059.5
44	58404.5
45	53444.5
46	48466.5
47	43635.5
48	38247.5
49	32357.5
50	27452.5
51	24001.5
52	21122.0
53	18537.5
54	16005.5
55	13869.5
56	12319.5
57	10601.0
58	8596.0
59	7044.0
60	5683.5
61	4546.5
62	3584.5
63	2460.0
64	1604.5
65	1167.5
66	853.5
67	583.5
68	427.0
69	440.5
70	468.0
71	374.5
72	327.5
73	292.0
74	213.0
75	150.0
76	83.5
77	54.5
78	33.5
79	12.5
80	12.0
81	10.5
82	5.5
83	3.0
84	2.5
85	2.0
86	0.5
87	1.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01160829419613262
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011888012128569551
20-24	7.832102108234057E-4
25-29	0.01648937211715706
30-34	0.006825117551461107
35-39	0.04036329765064909
40-44	0.047538062617656356
45-49	0.048181413862261296
50-54	0.04853106127780746
55-59	0.039454214370229064
60-64	0.04973384838728626
65-69	0.04032133996078355
70-74	0.00406989591695734
75-79	0.0018041806642182024
80-84	3.356615189243167E-4
85-89	0.0037482202946548695
90-94	0.0030629113601843903
95-99	0.0022517293561172915
100-101	6.992948310923265E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1430012.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.620492892575026
#Duplication Level	Relative count
1	100.0
2	7.377019378764558
3	2.7959230666682826
4	1.8572830947850643
5	1.465223661726031
6	1.1864797062281096
7	0.8889508319901106
8	0.7386714820694921
9	0.5992995043205314
10++	4.284173402978925
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	375490	2.6086402	5.197506	5
CAAAG	314255	2.113694	5.0482874	4
CTTTG	312420	2.0720682	8.280484	9
ACACA	298700	1.945088	7.662792	6
GAGAG	174380	1.8470111	5.6799064	7
GCTTT	261010	1.7311009	5.1907196	1
CACAT	255985	1.6591551	5.1381106	7
TACAC	254625	1.6503406	9.741984	5
TTGAG	218145	1.5014054	5.1257467	9
ATACA	325185	1.4279133	5.1118336	6
GACTC	140990	1.393214	5.442784	7
CCATG	140855	1.3918799	6.070429	9
GAGTC	134680	1.3746384	6.6214747	9
GTCTT	203770	1.3514671	6.2841797	1
AAGAC	199120	1.3392906	5.9581537	5
GATTG	181005	1.2457855	5.8765883	7
GTGTT	181355	1.242369	6.0125465	1
GACTT	185380	1.2352636	6.225751	7
TATGA	273525	1.2347888	5.782847	4
GTTCT	184965	1.2267464	5.55735	1
AGAAC	181185	1.2186589	5.27657	5
TCATA	275545	1.2042934	5.9341235	2
TATAC	264470	1.1558892	6.2492485	5
ACATG	168640	1.128987	5.3168626	8
TGGAC	109720	1.1198792	7.4281263	5
GTATG	159570	1.0982568	5.366016	3
GTGTA	157850	1.0864187	10.426113	1
GTCCA	109890	1.0858946	7.245028	1
ACACC	112795	1.084164	5.3314385	6
GGACT	98285	1.0031657	6.60168	6
ACACT	151915	0.98463005	5.12554	6
GAGTA	141905	0.981255	5.693517	1
AGACT	146315	0.97952884	5.839461	6
CATAC	137905	0.893825	5.0029798	3
ATACT	173260	0.75724787	5.143074	6
GTATA	167105	0.75437117	7.39459	1
>>END_MODULE