##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-176_CGAGGCTG-CTAAGCCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1485139	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.599252325876567	31.0	31.0	34.0	30.0	34.0
2	31.79144713053795	33.0	31.0	34.0	30.0	34.0
3	31.932174698799237	34.0	31.0	34.0	30.0	34.0
4	35.476974209148096	37.0	35.0	37.0	33.0	37.0
5	35.34074924973353	37.0	35.0	37.0	33.0	37.0
6	35.4855794642791	37.0	35.0	37.0	33.0	37.0
7	35.500293911883	37.0	35.0	37.0	33.0	37.0
8	35.55201836326432	37.0	35.0	37.0	33.0	37.0
9	37.35415405561365	39.0	37.0	39.0	34.0	39.0
10-14	37.46696140899943	39.2	37.2	39.4	34.0	39.4
15-19	38.49296584360117	40.0	38.0	41.0	34.0	41.0
20-24	38.388708127656734	40.0	38.0	41.0	34.0	41.0
25-29	38.1937297451619	40.0	38.0	41.0	33.2	41.0
30-34	37.92551808281918	40.0	38.0	41.0	32.8	41.0
35-39	37.60609101235642	40.0	37.2	41.0	31.8	41.0
40-44	37.56426435505364	40.0	37.0	41.0	31.8	41.0
45-49	37.39962952962652	40.0	37.0	41.0	31.4	41.0
50-54	36.69483287422928	38.8	35.4	40.2	30.8	40.6
55-59	37.31861542926285	39.8	36.0	41.0	31.6	41.0
60-64	36.84956909757268	39.0	35.2	41.0	31.0	41.0
65-69	36.163257715271094	37.6	35.0	40.0	30.2	41.0
70-74	35.30526597173732	36.4	34.2	39.2	29.2	40.8
75-79	34.33238774283081	35.2	34.0	37.6	28.8	39.4
80-84	33.37465382028215	35.0	33.2	36.2	27.0	38.0
85-89	32.57136496987824	35.0	33.0	35.2	25.6	36.6
90-94	31.938499224651697	35.0	32.0	35.0	24.6	36.0
95-99	31.375045433457746	34.4	31.2	35.0	22.2	35.0
100-101	30.414857464520157	34.0	30.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	36.0
7	138.0
8	313.0
9	415.0
10	518.0
11	646.0
12	1025.0
13	1488.0
14	1779.0
15	2277.0
16	2497.0
17	3098.0
18	3381.0
19	3912.0
20	4449.0
21	5246.0
22	6103.0
23	7002.0
24	8026.0
25	9752.0
26	11349.0
27	13676.0
28	16275.0
29	19889.0
30	24613.0
31	31176.0
32	40723.0
33	54808.0
34	78258.0
35	118630.0
36	201041.0
37	351090.0
38	391823.0
39	69655.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.87524753542282	23.23108155803847	10.547770631993297	24.34590027454541
2	15.522247719862136	23.578588897592663	39.53352565453984	21.365637728005364
3	15.95815759802898	27.592317442955345	32.572401149312256	23.87712380970342
4	11.374568780299878	18.109274203868416	43.933087364673455	26.583069651158247
5	10.999158050719025	38.97086855487825	38.62358131546156	11.406392078941165
6	25.908982561702004	43.442222255141324	19.249799006411592	11.398996176745076
7	24.150696673487623	33.530487923944655	26.602268220471082	15.71654718209664
8	21.43610821380691	38.0737419845303	24.1067755170201	16.383374284642688
9	23.066926575698503	14.751520905506906	24.91840809084696	37.26314442794762
10-14	20.685367359733018	25.707053600468132	32.552781299935425	21.054797739863425
15-19	20.07746222187859	31.378571084128975	28.40956755692277	20.134399137069664
20-24	19.996713488234892	29.791353645502568	30.036064078897834	20.17586878736471
25-29	20.003485260901673	30.237870403239835	29.54473712136842	20.213907214490067
30-34	19.87414279169323	30.0135154826075	29.65394654516199	20.458395180537277
35-39	20.045460869054622	30.205701139902068	29.532541974471826	20.21629601657148
40-44	19.761652165000466	29.85621524896566	30.065366255345783	20.31676633068809
45-49	19.838049973535817	29.684732792557167	30.066719370569945	20.410497863337074
50-54	19.467868353017497	29.358307084265334	30.47531945662308	20.69850510609409
55-59	19.630908817646606	29.35077951062215	30.46259421005766	20.555717461673588
60-64	19.385491511033482	28.81906523523059	30.718057436029216	21.077385817706716
65-69	19.825272283912	28.95274486022658	30.006310023509382	21.215672832352038
70-74	19.83196817747686	28.681418781543684	29.946836301306483	21.539776739672973
75-79	20.288160289329284	28.937561454797077	29.522261636193527	21.252016619680106
80-84	20.485622190805618	28.635644500723167	29.413899004558818	21.46483430391239
85-89	20.795038541030028	28.557237274672282	29.245553647007423	21.402170537290267
90-94	21.026817646709446	27.988205789657343	29.61959994876767	21.365376614865536
95-99	21.591604525654244	27.70687812388553	29.08532039119478	21.616196959265448
100-101	22.030961711984702	27.0008955867103	29.52621442904664	21.44192827225836
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.22114780996824
2	36.887885447867504
3	39.835281407732396
4	37.95763843145812
5	22.40555012966019
6	37.307978738447076
7	39.86724385558426
8	37.819482498449595
9	60.33007100364613
10-14	41.740165099596446
15-19	40.211861358948255
20-24	40.1725822755996
25-29	40.21739247539175
30-34	40.332537972230504
35-39	40.261756885626106
40-44	40.07841849568855
45-49	40.24854783687289
50-54	40.16637345911159
55-59	40.1866262793202
60-64	40.4628773287402
65-69	41.040945116264034
70-74	41.37174491714983
75-79	41.54017690900939
80-84	41.95045649471801
85-89	42.1972090783203
90-94	42.392194261574986
95-99	43.20780148491969
100-101	43.47288998424306
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.5
2	3.5
3	5.0
4	5.0
5	7.0
6	9.0
7	12.5
8	21.5
9	27.0
10	35.0
11	51.0
12	77.0
13	104.0
14	140.0
15	193.0
16	276.5
17	405.5
18	570.0
19	773.0
20	1095.5
21	1620.0
22	2372.0
23	3440.0
24	4774.0
25	6619.0
26	9035.0
27	12089.0
28	15631.5
29	19747.5
30	24581.0
31	29771.5
32	35232.5
33	41287.5
34	48739.5
35	57156.0
36	66869.0
37	76281.0
38	82566.5
39	85216.5
40	83672.5
41	79208.5
42	73891.5
43	69102.5
44	64968.0
45	60292.0
46	55554.5
47	50918.5
48	45399.5
49	39388.5
50	34405.5
51	30921.0
52	27359.0
53	24002.5
54	21028.5
55	17971.0
56	15746.0
57	13461.0
58	10815.0
59	8748.0
60	7080.0
61	5724.5
62	4578.5
63	3218.5
64	2229.0
65	1744.5
66	1285.5
67	910.0
68	667.5
69	690.0
70	734.0
71	598.0
72	495.5
73	422.0
74	316.0
75	222.0
76	149.5
77	100.5
78	66.5
79	41.5
80	25.5
81	19.5
82	17.5
83	12.5
84	11.0
85	10.5
86	10.5
87	10.5
88	9.0
89	6.5
90	5.5
91	5.0
92	1.5
93	1.0
94	2.0
95	5.5
96	7.0
97	4.0
98	1.5
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033868883653314606
2	0.03164686941761007
3	0.028616850005285698
4	0.02673150459317276
5	0.03292621094725814
6	0.001144674000211428
7	6.733376471831929E-4
8	0.0028280181181694107
9	2.693350588732772E-4
10-14	0.006464041412958652
15-19	0.02274534572184826
20-24	0.018988121650566042
25-29	0.0018718786591692765
30-34	0.001844945153281949
35-39	0.005494435201014855
40-44	0.012335545696396097
45-49	0.00760871541317008
50-54	0.0047402970361696785
55-59	0.0035956230359582503
60-64	0.009480594072339357
65-69	0.013466752943663859
70-74	0.017075842732565773
75-79	0.007029645036592535
80-84	0.010046197695973239
85-89	0.008362853578015257
90-94	0.009372860048790045
95-99	0.008712989154550517
100-101	0.005319367412747225
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1485139.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.092429184698098
#Duplication Level	Relative count
1	100.0
2	7.2155564244171835
3	2.9470700356776307
4	2.0569620253164556
5	1.5676164410341626
6	1.20350911490152
7	0.9365378036264113
8	0.8045794438199502
9	0.6390205757294365
10++	4.812814622941206
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	411200	3.6451871	6.228833	90-94
CTGTC	388245	3.4416971	5.775687	90-94
GCTGC	262360	3.415734	7.9268403	90-94
TCTCT	558640	3.371934	4.980638	90-94
GACGC	238285	3.1798773	8.295739	95-97
CTCTT	521305	3.146581	4.7359095	90-94
CGCTG	236115	3.0740435	7.7461863	85-89
GCCGA	223825	2.9869108	8.2001505	90-94
CTGCC	234625	2.9710922	7.7272162	90-94
ACACA	453115	2.9453514	7.165085	6
CGACG	220220	2.9388025	8.157657	95-97
GAAGG	286255	2.8181453	6.5545864	85-89
CCGAC	216090	2.804812	7.6699147	90-94
TGCCG	212520	2.766854	7.747517	90-94
TACAC	422160	2.677186	9.332877	5
AGAAG	379720	2.6090434	5.0992675	5
CTGAC	277020	2.5171268	6.0760255	95-97
TGACG	262515	2.4524074	6.1109605	95-97
GGCTT	260640	2.3754854	5.671583	95-97
GACGA	242845	2.3253853	6.293198	95-97
ACGCT	243690	2.2142756	5.519937	85-89
CAAAG	328835	2.1976132	5.040831	4
CGAAG	228075	2.1839538	6.070266	95-97
ATACA	479105	2.1801393	5.2652287	6
AAGGC	219660	2.1033752	5.4549065	90-94
AGGCT	222860	2.0819516	5.5048614	90-94
CTTTG	324750	2.0153048	8.30629	9
TATAC	427650	1.8985184	5.94554	5
GGTGG	128345	1.7662596	5.7203517	95-97
GAGAG	169415	1.6678699	5.4379244	7
AGGTG	165360	1.5882305	5.267251	95-97
GTGTA	230455	1.507126	10.382783	1
TTGAG	221650	1.4495431	5.2679234	9
CTCGG	107415	1.3984642	5.3717055	95-97
AAGAC	204785	1.3685839	5.893397	5
GAGTC	145090	1.3554265	7.167231	9
CCATG	146315	1.329483	5.411264	9
TATGA	276685	1.2628639	5.975474	4
GTCTT	203325	1.2617763	6.116735	1
GACTT	196840	1.2520802	6.4902825	7
TCATA	278530	1.236512	6.160838	2
TGAGT	187150	1.2239205	5.45827	8
GTGTT	184510	1.1772158	6.0411606	1
GATTG	178720	1.1687901	5.7467284	7
GTTCT	183545	1.1390272	5.297959	1
ACACC	123460	1.1184146	5.1794496	6
ACATG	165455	1.0787628	5.3279085	8
TGGAC	113380	1.0591927	6.774515	5
GTCCA	112970	1.0264955	7.3694415	1
ACACT	161585	1.0247136	5.301905	6
AGACT	152255	0.99269897	5.457328	6
GGACT	105580	0.9863253	6.2292314	6
GAGTA	146645	0.9830098	5.2870755	1
GTATG	140875	0.921292	5.0881557	3
GTATA	166405	0.7595166	7.2995186	1
>>END_MODULE