##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-176_CGAGGCTG-CTAAGCCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1485139	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.389278040641315	34.0	31.0	34.0	31.0	34.0
2	32.57848726617509	34.0	31.0	34.0	31.0	34.0
3	32.68622061638675	34.0	31.0	34.0	31.0	34.0
4	36.21845160621329	37.0	35.0	37.0	35.0	37.0
5	35.93245144057223	37.0	35.0	37.0	35.0	37.0
6	36.028386568529946	37.0	36.0	37.0	35.0	37.0
7	36.02761425024863	37.0	35.0	37.0	35.0	37.0
8	36.05010103431396	37.0	36.0	37.0	35.0	37.0
9	37.913643100073465	39.0	38.0	39.0	35.0	39.0
10-14	38.08536722825271	39.2	38.2	39.4	35.2	39.4
15-19	39.19623833190025	40.0	39.0	41.0	36.0	41.0
20-24	39.12099056047952	40.0	39.0	41.0	36.0	41.0
25-29	38.91797548916297	40.0	38.4	41.0	35.4	41.0
30-34	38.71276870380483	40.0	38.0	41.0	34.8	41.0
35-39	38.47834148857447	40.0	38.0	41.0	34.0	41.0
40-44	38.23314531501766	40.0	38.0	41.0	33.6	41.0
45-49	38.048298240097395	40.0	37.8	41.0	33.0	41.0
50-54	37.99302502998036	40.0	37.2	41.0	33.0	41.0
55-59	37.588675807449675	39.4	36.4	41.0	32.8	41.0
60-64	37.01496600654888	38.8	35.2	40.4	31.8	41.0
65-69	36.25982766596258	37.6	35.0	40.0	31.0	41.0
70-74	35.67171382611325	36.4	34.6	39.0	31.0	40.8
75-79	34.48508119442019	35.2	33.4	37.4	29.4	39.2
80-84	34.299383424716474	35.0	34.0	36.4	30.0	38.0
85-89	33.68927662663226	35.0	34.0	35.4	29.8	36.6
90-94	33.28512092134137	35.0	34.0	35.0	29.0	36.0
95-99	33.01979976285048	35.0	33.2	35.0	29.0	35.2
100-101	32.652326819240486	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	8.0
14	22.0
15	52.0
16	128.0
17	284.0
18	509.0
19	791.0
20	1138.0
21	1790.0
22	2504.0
23	3548.0
24	4728.0
25	6142.0
26	7832.0
27	10305.0
28	13190.0
29	16481.0
30	21026.0
31	26940.0
32	34804.0
33	46833.0
34	67342.0
35	107323.0
36	197069.0
37	373785.0
38	455878.0
39	84630.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.292633214803466	23.343471553841088	10.89790248589526	24.465992745460188
2	15.72015407822433	23.465143842258378	39.34826527313867	21.46643680637862
3	16.164749562162196	27.172271417018877	32.81154154594284	23.85143747487609
4	11.46795013800055	18.118304077934795	43.74506359337409	26.668682190690568
5	10.799931858230105	39.17323563652965	38.72593743750585	11.300895067734402
6	25.96356300656033	43.61086740029047	19.116459806119156	11.309109787030035
7	24.06892553491626	33.66984504480725	26.482234996185543	15.778994424090945
8	21.336925365235174	38.23945098741599	23.95883482960181	16.464788817747024
9	22.906004084466165	14.636003767997474	24.889589459303135	37.56840268823322
10-14	20.609249369924296	25.71252926493749	32.549168798341434	21.129052566796776
15-19	19.99114953197023	31.432978578419835	28.383553320164467	20.19231856944547
20-24	19.93782089800672	29.817176848990645	30.025859029638635	20.219143223364
25-29	19.973654685115573	30.303940887363	29.449603810103618	20.272800617417808
30-34	19.847772471075196	30.07864702921847	29.596734149014956	20.476846350691382
35-39	20.032257595165564	30.21013241996091	29.518959824582485	20.23865016029104
40-44	19.732649291762872	29.85934958834765	30.051369136293328	20.356631983596152
45-49	19.83802695167499	29.695481022282237	30.040060096207288	20.42643192983548
50-54	19.402713800621036	29.33496107657404	30.502562806372342	20.759762316432585
55-59	19.522548220134745	29.26331788584359	30.439278755496087	20.774855138525574
60-64	19.174761630994123	28.825326215935572	30.562647458549254	21.437264694521062
65-69	19.543046233598545	29.023700490489233	29.76125985151542	21.6719934243968
70-74	19.43789637445815	28.82776588770371	29.536021357960777	22.19831637987736
75-79	19.92018684568357	28.998990383663635	28.959087846586794	22.121734924065997
80-84	19.916268981587283	28.748884109380914	28.67912621640326	22.65572069262854
85-89	20.053335322881114	28.5622343519396	28.623981260183694	22.76044906499559
90-94	20.018285597930163	27.90115865348617	29.024595731798303	23.055960016785367
95-99	20.19195936182333	27.68163349956165	28.76264724538181	23.363759893233212
100-101	20.068902664633633	26.823760881560876	29.58151714469728	23.525819309108208
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.75862596026366
2	37.18659088460296
3	40.016187037038286
4	38.136632328691114
5	22.100826925964505
6	37.27267279359037
7	39.84791995900721
8	37.8017141829822
9	60.47440677269938
10-14	41.73830193672107
15-19	40.1834681014157
20-24	40.15696412137072
25-29	40.24645530253338
30-34	40.32461882176658
35-39	40.270907755456605
40-44	40.08928127535902
45-49	40.26445888151047
50-54	40.16247611705362
55-59	40.29740335866032
60-64	40.61202632551518
65-69	41.21503965799535
70-74	41.6362127543355
75-79	42.04192176974957
80-84	42.57198967421583
85-89	42.81378438787671
90-94	43.07424561471553
95-99	43.55571925505654
100-101	43.59472197374185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	1.5
7	3.0
8	3.5
9	6.5
10	10.5
11	18.5
12	38.5
13	64.5
14	105.0
15	167.5
16	245.5
17	386.5
18	556.5
19	750.5
20	1084.0
21	1600.5
22	2346.5
23	3401.5
24	4804.5
25	6711.0
26	9111.0
27	12101.0
28	15657.5
29	19662.5
30	24399.5
31	29509.5
32	34727.5
33	40732.5
34	47789.5
35	55994.5
36	65929.0
37	75034.5
38	81148.0
39	83228.5
40	81316.0
41	77503.5
42	71998.0
43	66688.5
44	63261.5
45	59448.0
46	55437.5
47	51802.5
48	47515.5
49	41984.0
50	36715.5
51	33346.5
52	29710.0
53	25956.0
54	22608.0
55	19333.0
56	16862.0
57	14257.5
58	11461.0
59	9261.0
60	7404.5
61	5979.5
62	4645.0
63	3114.0
64	2117.0
65	1694.0
66	1243.0
67	847.0
68	620.5
69	672.0
70	725.0
71	577.0
72	488.0
73	415.5
74	297.0
75	218.5
76	133.0
77	76.5
78	41.0
79	11.5
80	7.0
81	6.5
82	2.5
83	1.0
84	0.5
85	0.5
86	0.5
87	0.5
88	1.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012052743884579154
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.001333208541422722
20-24	6.598708942395291E-4
25-29	0.01649677235598823
30-34	0.006652575954169946
35-39	0.03968652092497739
40-44	0.04759150490290808
45-49	0.04842644358541524
50-54	0.047914706973556015
55-59	0.03924211807783648
60-64	0.049746185373894296
65-69	0.04182773464301995
70-74	0.0035956230359582503
75-79	0.0013736088002537137
80-84	1.616010353239663E-4
85-89	0.0029896191534933766
90-94	0.0024105487769158305
95-99	0.002222014235704537
100-101	3.366688235915965E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1485139.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.898394110302664
#Duplication Level	Relative count
1	100.0
2	7.422078919084907
3	3.0419161676646707
4	2.029172424382005
5	1.5826807922616306
6	1.2713035467526486
7	0.9568555197297712
8	0.8340242591739597
9	0.6909258406264395
10++	5.002303086135422
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	398150	5.2386084	14.8374	95-97
GTCTC	411990	3.5803611	6.1085596	90-94
GAGCC	266390	3.504993	8.091774	95-97
CTCCG	288005	3.4708793	7.949246	95-97
CTGTC	389725	3.3868692	5.6355367	75-79
CGAGC	256400	3.3735504	8.115406	85-89
TCTCT	558240	3.266164	4.8717885	90-94
AGCCC	253890	3.118825	7.326575	90-94
GCCCA	253780	3.1174736	7.661334	90-94
CTCTT	522470	3.05688	4.666774	80-84
TCTCC	370375	3.0050936	5.8593493	95-97
ATCTC	477860	2.849861	7.064599	95-97
ACACA	454145	2.814034	7.162476	6
CCCAC	240250	2.755402	7.131742	90-94
TCCGA	306015	2.710746	6.0434	95-97
AGAAG	380350	2.703746	5.152829	5
CCACG	219435	2.695574	7.402818	90-94
CGAGG	190160	2.6798604	8.25214	90-94
GGCTG	189825	2.6244633	8.3150835	95-97
TACAC	423640	2.5752883	9.039924	5
GAGGC	181440	2.5569727	8.161939	90-94
CGAGA	256160	2.4773486	6.441667	95-97
GAGAC	254140	2.4578133	6.622991	95-97
GACCG	183870	2.4192464	7.508028	85-89
ACGAG	240955	2.3302996	6.1493616	95-97
CAAAG	332105	2.204114	5.274237	4
ATACA	480095	2.145175	5.067431	6
CACGA	231325	2.0886927	5.50436	90-94
AGGCT	214275	2.033019	5.837321	90-94
CTTTG	323735	2.0287592	8.951498	9
AGACC	224540	2.0274296	5.8331895	95-97
TATAC	431400	1.8910792	6.0044074	5
GCTGA	190285	1.8054042	5.539078	95-97
ACCGA	197285	1.7813368	5.0753307	85-89
GAGAG	168465	1.7450559	5.544863	7
GCTTT	273775	1.7156736	5.117343	1
TTGAG	220660	1.5097123	5.8053813	9
GTCTT	221720	1.389459	6.0593715	1
GAGTC	145485	1.3803467	6.560117	9
AAGAC	206285	1.3690721	5.8405995	5
CCATG	148940	1.3193421	5.433238	9
GTATG	191430	1.3097265	5.4504237	3
TATGA	276615	1.2987607	6.2650414	4
TGAGT	186370	1.2751069	5.2381124	8
GACTT	196960	1.258128	6.7797656	7
TCATA	280970	1.2316563	6.3344283	2
GTGTT	183325	1.2305135	5.9953685	1
GATTG	177930	1.217362	6.087357	7
GTGTA	171370	1.17248	10.898521	1
GTTCT	183230	1.1482526	5.235856	1
ACATG	166845	1.0863403	5.4142575	8
TGGAC	112960	1.0717528	7.323777	5
GCCGT	82610	1.0663406	6.179712	95-97
CCGTC	86635	1.0440779	5.0995164	95-97
GTCCA	114495	1.0142211	6.894182	1
GGACT	105440	1.0004036	6.932746	6
AGACT	153535	0.999678	5.5310674	6
GAGTA	143335	0.9996059	5.5866075	1
TGCCG	76405	0.9862456	6.440503	95-97
TGTAT	213605	0.9839179	5.050212	2
ACACT	159770	0.97123456	5.205251	6
GTATA	164920	0.77433115	7.2036314	1
ATACT	170955	0.7493961	5.0628314	6
>>END_MODULE