##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-175_CGAGGCTG-AAGGAGTA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2365119	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.637483779885915	33.0	31.0	34.0	30.0	34.0
2	31.835250573015564	33.0	31.0	34.0	30.0	34.0
3	31.971408203984662	34.0	31.0	34.0	30.0	34.0
4	35.51649367325703	37.0	35.0	37.0	33.0	37.0
5	35.38648372449758	37.0	35.0	37.0	33.0	37.0
6	35.52407215028081	37.0	35.0	37.0	33.0	37.0
7	35.53842914458004	37.0	35.0	37.0	33.0	37.0
8	35.58724233326103	37.0	35.0	37.0	33.0	37.0
9	37.38670950594875	39.0	37.0	39.0	34.0	39.0
10-14	37.51294932728543	39.2	37.2	39.4	34.0	39.4
15-19	38.55165461019086	40.0	38.0	41.0	34.0	41.0
20-24	38.45653449149916	40.0	38.0	41.0	34.0	41.0
25-29	38.27166616140668	40.0	38.0	41.0	33.6	41.0
30-34	38.010432202354295	40.0	38.0	41.0	33.0	41.0
35-39	37.69804513007591	40.0	37.6	41.0	32.2	41.0
40-44	37.65808206690657	40.0	37.6	41.0	31.8	41.0
45-49	37.504422229917395	40.0	37.0	41.0	31.6	41.0
50-54	36.824493313021456	39.0	36.4	40.2	31.0	40.6
55-59	37.47678336692572	40.0	36.6	41.0	32.0	41.0
60-64	37.03940452890531	39.0	35.6	41.0	31.0	41.0
65-69	36.37011727528297	38.2	35.0	40.2	30.6	41.0
70-74	35.509321180033645	36.6	34.6	39.4	29.6	41.0
75-79	34.51567671647811	35.4	34.0	38.0	29.0	39.6
80-84	33.54398954132964	35.0	33.6	36.4	27.6	38.2
85-89	32.71569202226188	35.0	33.0	35.4	26.0	36.8
90-94	32.06262788468572	35.0	32.2	35.0	24.8	36.0
95-99	31.467474067901026	34.6	31.4	35.0	22.6	35.2
100-101	30.500718357089006	34.0	30.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	4.0
6	61.0
7	246.0
8	513.0
9	665.0
10	853.0
11	1140.0
12	1580.0
13	2311.0
14	2849.0
15	3356.0
16	4038.0
17	4577.0
18	5478.0
19	6155.0
20	7066.0
21	8455.0
22	9405.0
23	10996.0
24	12784.0
25	14983.0
26	17539.0
27	20748.0
28	25059.0
29	30599.0
30	37644.0
31	47251.0
32	61230.0
33	81840.0
34	115864.0
35	177790.0
36	303986.0
37	549658.0
38	661063.0
39	137238.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.955944645418285	23.82650240809095	10.899915874367313	24.317637072123453
2	14.802541563908541	24.428949602240934	40.18760169195105	20.580907141899473
3	15.193896608775972	28.7599769249248	33.51257117916341	22.533555287135815
4	10.847906929730813	18.830421574519356	45.19797690244801	25.12369459330182
5	10.494866255335491	39.638743065765055	39.2219699571469	10.644420721752562
6	24.619126222787482	44.61905857202776	19.927038655644104	10.834776549540653
7	22.746604913346488	34.48733229631277	27.866284386892954	14.899778403447792
8	20.64862542773702	38.520538353523776	25.050495483629824	15.780340735109382
9	23.045491772260814	14.92231436267842	25.439504056684072	36.5926898083767
10-14	20.06718918342943	26.18376431945687	33.479630133904784	20.26941636320891
15-19	19.485602471985306	31.823173745745905	29.12310225629897	19.56812152596982
20-24	19.42586237870064	30.377397780030968	30.608739000888757	19.58800084037963
25-29	19.417955033138483	30.75794213714153	30.214103146834663	19.60999968288532
30-34	19.305368399644664	30.6145286355943	30.29657758021818	19.783525384542855
35-39	19.40383781100502	30.746234451334757	30.221793374528644	19.62813436313158
40-44	19.142357266255647	30.34735696982619	30.738567739121102	19.771718024797064
45-49	19.142363188040875	30.13505060052793	30.876778980638715	19.84580723079248
50-54	18.734745348911748	29.85985213464059	31.209170473405596	20.19623204304207
55-59	18.83893632347397	29.846664412718155	31.163693658968572	20.150705604839303
60-64	18.662435987351937	29.36855851123704	31.287612306767492	20.681393194643526
65-69	19.106159822658896	29.31835294557431	30.631784034378235	20.943703197388565
70-74	18.94285732409465	29.081551743459517	30.59909206049829	21.376498871947543
75-79	19.060152862118127	29.105266620740487	30.40971438439331	21.42486613274808
80-84	19.049223082095583	28.800134569753556	30.489698758096743	21.660943590054117
85-89	19.312073422314803	28.511546319319482	30.544437992753092	21.63194226561262
90-94	19.562603080067515	28.012500437648942	30.819787922513047	21.605108559770496
95-99	20.099298434605203	27.52777968764496	30.504001122409914	21.868920755339925
100-101	20.41307935941473	26.90197668619385	30.890719149968067	21.794224804423354
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.27358171754175
2	35.383448705808014
3	37.727451895911784
4	35.97160152303263
5	21.139286977088055
6	35.453902772328135
7	37.64638331679428
8	36.42896616284641
9	59.63818158063751
10-14	40.336605546638346
15-19	39.053723997955125
20-24	39.01386321908027
25-29	39.02795471602381
30-34	39.08889378418752
35-39	39.03197217413659
40-44	38.91407529105271
45-49	38.988170418833356
50-54	38.93097739195382
55-59	38.989641928313276
60-64	39.34382918199547
65-69	40.04986302004746
70-74	40.31935619604219
75-79	40.4850189948662
80-84	40.7101666721497
85-89	40.94401568792742
90-94	41.16771163983801
95-99	41.968219189945124
100-101	42.20730416383808
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	7.5
2	12.5
3	13.0
4	15.0
5	24.5
6	27.0
7	40.0
8	58.0
9	73.0
10	106.5
11	142.5
12	207.5
13	306.5
14	434.0
15	613.5
16	852.5
17	1243.5
18	1792.0
19	2609.0
20	3806.0
21	5523.0
22	7917.0
23	10946.5
24	14704.0
25	19208.0
26	24286.5
27	29861.5
28	36538.0
29	43620.5
30	51068.0
31	58873.5
32	66587.0
33	75237.0
34	84792.0
35	95378.5
36	107498.5
37	118472.5
38	125955.0
39	128477.5
40	125770.0
41	121017.5
42	114519.0
43	107038.0
44	99793.0
45	92290.5
46	84616.5
47	75913.0
48	66834.5
49	57400.0
50	48733.5
51	42250.5
52	36195.0
53	30929.5
54	26295.5
55	21955.5
56	18651.0
57	15594.5
58	12641.0
59	10234.0
60	8300.0
61	6671.5
62	5245.5
63	3820.0
64	2757.5
65	2202.5
66	1730.0
67	1300.5
68	1025.0
69	1045.0
70	1035.0
71	836.5
72	691.5
73	570.5
74	455.0
75	358.5
76	266.0
77	194.0
78	128.0
79	85.5
80	66.0
81	51.5
82	46.0
83	40.5
84	36.0
85	27.0
86	18.0
87	15.5
88	14.5
89	13.5
90	8.0
91	5.5
92	9.5
93	9.5
94	6.0
95	6.5
96	7.0
97	8.0
98	6.0
99	2.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033740374162991374
2	0.03162631563147563
3	0.028032416127898852
4	0.026468012814577193
5	0.03226053319093035
6	0.0013107162895397652
7	4.228117063031501E-4
8	0.0023677455552976404
9	4.228117063031501E-4
10-14	0.006553581447698825
15-19	0.024277848175926875
20-24	0.020015906176391124
25-29	0.0018603715077338604
30-34	0.0016066844839519704
35-39	0.005285146328789376
40-44	0.012971863149380643
45-49	0.007745910459473709
50-54	0.00433804810667032
55-59	0.003560074567072524
60-64	0.009927618863997964
65-69	0.01354688706995293
70-74	0.017927216347253562
75-79	0.006942568217497724
80-84	0.010519555252822374
85-89	0.008126440995146545
90-94	0.00887058959824009
95-99	0.008777571022853394
100-101	0.005306286914104534
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2365119.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	24.657509290971365
#Duplication Level	Relative count
1	100.0
2	7.455811591749885
3	2.303721256377397
4	1.5843750755615735
5	1.2440457479991296
6	0.9998307420751021
7	0.8481031022559663
8	0.7187416882269023
9	0.5954252000870469
10++	5.148463379838963
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	468390	4.210119	9.526098	90-94
GTCTC	710805	4.0458374	6.398896	60-64
GACGC	431790	4.0355697	9.647521	85-89
CGCTG	429215	3.8579946	9.326593	75-79
CTGTC	667150	3.7973568	6.1684675	55-59
GCCGA	402125	3.758316	9.838961	80-84
CGACG	394060	3.6829398	9.932279	95-97
CTGCC	422860	3.5934553	8.983994	90-94
TGCCG	389225	3.4985452	9.278487	90-94
TCTCT	965900	3.4814591	5.064555	60-64
CCGAC	385620	3.4073815	9.088585	80-84
CTCTT	891255	3.2124112	4.7792673	60-64
ACACA	768155	3.1125379	6.814395	6
TGTCT	812420	3.0972826	4.642667	65-69
TGACG	472295	2.9565685	6.6561723	85-89
CTGAC	492695	2.9159606	6.1195693	85-89
GACGA	435035	2.831681	7.27406	95-97
TACAC	699575	2.72618	7.848485	5
AGAAG	574740	2.6054301	5.268695	5
ACGCT	440035	2.6042984	6.211049	75-79
CCTTG	412965	2.350559	6.096944	90-94
CTCCT	431765	2.323455	5.9599705	90-94
ACTCC	403235	2.2562675	5.8698378	90-94
CAAAG	483870	2.0737936	5.143335	4
GGTGG	197430	1.9853729	7.5959973	95-97
AGAGA	432330	1.9598523	5.550975	8
TATAC	731015	1.908046	5.756994	5
GAGAG	270255	1.8606511	6.263467	7
CTTTG	480645	1.8324184	8.160794	9
GTGTA	383010	1.6059315	9.107923	1
CTCGG	178350	1.6030972	7.000296	95-97
CGGTG	142630	1.3560282	6.8494554	95-97
AAGAC	309690	1.3272848	5.877207	5
GAGTC	204975	1.2831442	6.514847	9
CGCCG	92350	1.2393138	5.471955	95-97
GTCTT	309340	1.1793327	6.080656	1
GATTG	281015	1.1782744	5.5611544	7
TATGA	416695	1.1504081	5.5897756	4
GACTT	289675	1.1483039	5.8901353	7
GTGTT	282465	1.1390321	5.6081214	1
TCATA	417810	1.0905395	5.337912	2
TGGAC	169510	1.0611333	6.99625	5
GTCCA	166735	0.9868026	7.2548275	1
GGACT	154315	0.9660125	6.5752983	6
AGACT	229255	0.9449529	5.0570154	6
GAGTA	208655	0.9096855	5.111607	1
GTATA	254505	0.70263535	7.2117014	1
>>END_MODULE