##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-175_CGAGGCTG-AAGGAGTA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2365119	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42807909454027	34.0	31.0	34.0	31.0	34.0
2	32.61220471358946	34.0	31.0	34.0	31.0	34.0
3	32.720404766102675	34.0	31.0	34.0	31.0	34.0
4	36.24431540231168	37.0	35.0	37.0	35.0	37.0
5	35.97038795933735	37.0	35.0	37.0	35.0	37.0
6	36.06009507344028	37.0	36.0	37.0	35.0	37.0
7	36.0537034288761	37.0	36.0	37.0	35.0	37.0
8	36.07533658982909	37.0	36.0	37.0	35.0	37.0
9	37.9441630632539	39.0	38.0	39.0	35.0	39.0
10-14	38.121286497635005	39.2	38.2	39.4	35.2	39.4
15-19	39.24660289820512	40.0	39.0	41.0	36.0	41.0
20-24	39.18024234721382	40.0	39.0	41.0	36.0	41.0
25-29	38.986970042522174	40.0	38.8	41.0	35.8	41.0
30-34	38.78830249133342	40.0	38.0	41.0	35.0	41.0
35-39	38.56182399278852	40.0	38.0	41.0	34.2	41.0
40-44	38.32945192186947	40.0	38.0	41.0	33.8	41.0
45-49	38.16297387150498	40.0	38.0	41.0	33.4	41.0
50-54	38.123118202509055	40.0	37.6	41.0	33.2	41.0
55-59	37.75574641275979	39.8	36.6	41.0	33.0	41.0
60-64	37.20693258986123	39.0	35.6	40.8	31.8	41.0
65-69	36.46867443033521	37.8	35.0	40.0	31.0	41.0
70-74	35.875481191432655	36.6	35.0	39.2	31.0	41.0
75-79	34.656358770954014	35.2	33.6	37.4	30.2	39.2
80-84	34.4294262571989	35.0	34.0	36.6	30.8	38.4
85-89	33.7834181705022	35.0	34.0	35.6	30.0	36.8
90-94	33.357049772125634	35.0	34.0	35.0	29.2	36.0
95-99	33.075232662711684	35.0	33.4	35.0	29.0	35.4
100-101	32.71276878668684	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	8.0
14	29.0
15	80.0
16	191.0
17	434.0
18	750.0
19	1217.0
20	1864.0
21	2731.0
22	3907.0
23	5434.0
24	7449.0
25	9612.0
26	12394.0
27	15914.0
28	20342.0
29	25477.0
30	32130.0
31	40877.0
32	52871.0
33	70893.0
34	101541.0
35	160333.0
36	292405.0
37	572953.0
38	763440.0
39	169676.0
40	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.36295848115888	23.903575253507327	11.271568153653156	24.46189811168064
2	14.920934952903492	24.446460097470215	39.92646390324865	20.70614104637764
3	15.325190825493348	28.521355585067816	33.67386588158989	22.47958770784895
4	11.085911533415443	18.80197148642415	44.88514108592422	25.226975894236187
5	10.350599694983636	39.73960718255614	39.34482789238089	10.564965230079332
6	24.63529319243556	44.77783147486448	19.806783506453588	10.780091826246375
7	22.696786081376878	34.4680331095391	27.935296278961015	14.899884530123009
8	20.531102240521513	38.65691324622566	25.02296924594492	15.789015267307905
9	22.956603874900164	14.778199321048962	25.345912827219262	36.91928397683161
10-14	20.00454945395982	26.160916216055092	33.5276068561455	20.306927473839583
15-19	19.42119191163077	31.85121374415943	29.1199236495721	19.607670694637694
20-24	19.40892568088772	30.408663705048696	30.60630409593897	19.576106518124618
25-29	19.396857404538522	30.790671050351335	30.160140715905786	19.652330829204352
30-34	19.276580749136414	30.63201381436912	30.25847649374547	19.832928942749
35-39	19.389187487622227	30.763964856894116	30.209288323596223	19.637559331887438
40-44	19.11969835324269	30.379902015735993	30.709624047338785	19.79077558368253
45-49	19.10493806792502	30.12881526405124	30.860483662592575	19.905763005431165
50-54	18.67785264426087	29.85947869463838	31.18677852644261	20.27589013465814
55-59	18.726668594920998	29.78114087742114	31.111171081193184	20.381019446464673
60-64	18.414644123734504	29.40288946912124	31.048920152735292	21.13354625440896
65-69	18.802276732021067	29.473453480936335	30.213668022855302	21.5106017641873
70-74	18.80389533029193	29.361003873411068	29.815819320646114	22.019281475650885
75-79	19.341746380388994	29.467507430166247	29.182477914990407	22.008268274454352
80-84	19.479408198160257	29.19907522506296	28.912188662205153	22.40932791457163
85-89	19.668512884625923	28.931965616642884	28.91194068018485	22.487580818546338
90-94	19.688869333864172	28.297735004402842	29.250580174525492	22.762815487207494
95-99	19.840849035159643	27.872838884784723	29.247213788381863	23.03909829167377
100-101	19.748418525329704	27.142974020117812	29.9771237677943	23.131483686758187
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	64.82485659283952
2	35.62707599928113
3	37.8047785333423
4	36.31288742765163
5	20.915564925062966
6	35.41538501868193
7	37.59667061149989
8	36.320117507829416
9	59.87588785173177
10-14	40.311476927799404
15-19	39.02886260626847
20-24	38.98503219901234
25-29	39.049188233742875
30-34	39.109509691885414
35-39	39.02674681950966
40-44	38.91047393692522
45-49	39.01070107335619
50-54	38.953742778919015
55-59	39.10768804138567
60-64	39.54819037814347
65-69	40.31287849620836
70-74	40.823176805942815
75-79	41.35001465484335
80-84	41.88873611273189
85-89	42.15609370317226
90-94	42.451684821071666
95-99	42.87994732683341
100-101	42.87990221208789
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	2.0
5	2.5
6	2.0
7	4.5
8	12.0
9	26.0
10	42.0
11	63.0
12	118.5
13	206.0
14	316.5
15	505.5
16	761.5
17	1143.0
18	1760.0
19	2598.0
20	3801.0
21	5625.5
22	7878.0
23	10932.5
24	14747.0
25	19088.5
26	24305.5
27	29943.5
28	36359.0
29	43012.0
30	50109.0
31	57975.0
32	65498.5
33	73503.0
34	82842.0
35	92970.5
36	104203.0
37	114677.5
38	121762.5
39	123852.0
40	120599.0
41	115173.5
42	107902.0
43	100555.0
44	95013.5
45	89240.0
46	83766.5
47	78515.0
48	71782.5
49	63459.5
50	55408.0
51	48921.5
52	42826.0
53	36842.0
54	31078.5
55	25927.0
56	21956.5
57	18215.0
58	14665.0
59	11732.5
60	9157.0
61	7207.5
62	5630.5
63	3957.5
64	2844.5
65	2323.5
66	1818.0
67	1309.5
68	988.0
69	1024.5
70	1054.5
71	839.5
72	705.0
73	603.0
74	459.5
75	342.5
76	243.5
77	156.0
78	83.0
79	50.0
80	33.5
81	19.5
82	12.5
83	8.5
84	7.0
85	5.0
86	4.0
87	2.0
88	0.5
89	1.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012430664165312612
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010232043292536233
20-24	6.511300277068511E-4
25-29	0.01738601736318553
30-34	0.006815724705606779
35-39	0.04238264543982776
40-44	0.05024694317706636
45-49	0.051016460482538085
50-54	0.05103337295079022
55-59	0.04131715993994383
60-64	0.0523610017085821
65-69	0.042010571138280994
70-74	0.003035788051256617
75-79	0.0012938038212876393
80-84	1.2684351189094504E-4
85-89	0.0028666633687353575
90-94	0.0023170081505412624
95-99	0.0018519152736077972
100-101	2.1140585315157505E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2365119.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.340777558384975
#Duplication Level	Relative count
1	100.0
2	7.677014555138073
3	2.4325441700409782
4	1.592918201826384
5	1.2773744816936003
6	1.034788847140651
7	0.8931285643064726
8	0.7314048079378397
9	0.6092000145916171
10++	5.348435657048359
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	723630	6.523958	17.805094	90-94
GAGCC	469705	4.2346725	9.671886	75-79
CGAGC	454915	4.1013317	9.554736	75-79
CTCCG	500320	4.090456	8.53853	75-79
GTCTC	711040	4.0245237	6.407486	60-64
CTGTC	668510	3.7838016	6.17206	55-59
AGCCC	442075	3.6911583	8.582165	90-94
GCCCA	437185	3.6503286	8.838035	90-94
TCTCT	968030	3.5129972	5.1109076	60-64
TCTCC	647250	3.392849	6.2306566	70-74
CCACG	394945	3.2976408	8.66359	80-84
CCCAC	424515	3.2827046	8.167588	80-84
CGAGG	336445	3.2751915	10.316409	90-94
CTCTT	891815	3.236412	4.8322334	60-64
GGCTG	336150	3.2041502	10.577597	95-97
TGTCT	814850	3.1929686	4.766993	65-69
ATCTC	860370	3.1887255	7.4936376	95-97
GAGGC	327005	3.1832955	10.321129	90-94
TCCGA	527215	3.047551	6.36643	85-89
GACCG	332035	2.9934947	9.236175	85-89
ACACA	772735	2.9870887	6.4548182	6
GAGAC	454000	2.893942	7.244254	85-89
CGAGA	447145	2.8502464	7.2096047	95-97
ACGAG	426605	2.719318	6.9458637	95-97
TACAC	704010	2.6647317	8.118823	5
AGAAG	577925	2.6046236	5.5218167	5
CACGA	412765	2.4367385	6.280007	80-84
AGACC	401200	2.3684654	6.423446	85-89
AGGCT	369045	2.303406	6.9395375	90-94
GCTGA	338545	2.1130395	6.7598066	95-97
ACCGA	355630	2.0994449	6.1067986	90-94
AGAGA	430895	1.9419806	5.515261	8
TATAC	735040	1.9261149	5.930692	5
CTTTG	481625	1.8872352	8.48285	9
GAGAG	268710	1.8494635	6.5139594	7
TGGAG	250665	1.6893235	5.005889	5
TCTCG	295075	1.6701397	5.390104	90-94
GCTTT	400900	1.5709163	5.0866733	1
CTCGT	254715	1.4417002	5.3039284	90-94
AGGAG	208425	1.4345369	5.2125025	7
GTCTT	352020	1.3793813	5.9721384	1
AAGAC	311445	1.2999506	5.775675	5
GAGTC	204690	1.2775792	6.5577283	9
GCCGT	141915	1.2527955	7.917171	95-97
ATGAG	279325	1.2326517	5.034494	7
TGCCG	137760	1.216116	8.068409	95-97
GATTG	277360	1.1984823	5.507845	7
CCGTC	146185	1.1951617	6.809065	95-97
ATGCC	206560	1.1940142	5.505582	95-97
GTGTT	280180	1.1854467	5.9191484	1
TATGA	413810	1.170847	5.4434485	4
GACTT	286505	1.1465474	6.127386	7
TCATA	423415	1.109526	5.4893827	2
TGGAC	167745	1.0469857	7.0842834	5
GTGTA	240005	1.0370699	9.114765	1
GTCCA	169230	0.97822917	6.909533	1
GGACT	152150	0.94964904	6.5547023	6
AGACT	230750	0.943071	5.387846	6
GAGTA	207495	0.91566837	5.178625	1
GTATA	255345	0.7224812	7.0495567	1
>>END_MODULE