##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-174_CGAGGCTG-ACTGCATA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3154287	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.623529501278735	31.0	31.0	34.0	30.0	34.0
2	31.828292733032853	33.0	31.0	34.0	30.0	34.0
3	31.9733099746472	34.0	31.0	34.0	30.0	34.0
4	35.52509235843156	37.0	35.0	37.0	33.0	37.0
5	35.3780743477052	37.0	35.0	37.0	33.0	37.0
6	35.50888172192321	37.0	35.0	37.0	33.0	37.0
7	35.52534883477629	37.0	35.0	37.0	33.0	37.0
8	35.58109740806718	37.0	35.0	37.0	33.0	37.0
9	37.395337202987555	39.0	37.0	39.0	34.0	39.0
10-14	37.51306574195691	39.2	37.2	39.4	34.0	39.4
15-19	38.53375225526403	40.0	38.0	41.0	34.0	41.0
20-24	38.420293017090714	40.0	38.0	41.0	34.0	41.0
25-29	38.20973202501865	40.0	38.0	41.0	33.4	41.0
30-34	37.91088325190448	40.0	38.0	41.0	32.8	41.0
35-39	37.549977982345936	40.0	37.0	41.0	31.6	41.0
40-44	37.48167310076731	40.0	37.0	41.0	31.8	41.0
45-49	37.297847722797584	40.0	36.6	41.0	31.0	41.0
50-54	36.55425305306714	38.6	35.4	40.2	30.4	40.6
55-59	37.18424372924848	39.6	35.8	41.0	31.0	41.0
60-64	36.73371433861281	39.0	35.0	41.0	31.0	41.0
65-69	36.07516297660929	37.6	35.0	40.0	30.0	41.0
70-74	35.250149907094695	36.4	34.2	39.2	29.0	41.0
75-79	34.286047908766704	35.2	34.0	37.6	28.6	39.4
80-84	33.30573121596101	35.0	33.2	36.2	26.8	38.0
85-89	32.39895957469945	35.0	32.6	35.2	25.2	36.6
90-94	31.63470147136262	35.0	31.6	35.0	22.8	36.0
95-99	30.86162457633056	34.2	30.6	35.0	19.2	35.0
100-101	29.67589030421138	33.5	28.5	35.0	14.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	5.0
6	96.0
7	332.0
8	758.0
9	995.0
10	1271.0
11	1655.0
12	2319.0
13	3152.0
14	3875.0
15	4603.0
16	5501.0
17	6426.0
18	7567.0
19	8904.0
20	10326.0
21	11875.0
22	13591.0
23	15903.0
24	18705.0
25	21780.0
26	25581.0
27	30593.0
28	37033.0
29	44947.0
30	56032.0
31	70024.0
32	90622.0
33	122452.0
34	172632.0
35	259693.0
36	421819.0
37	709884.0
38	806320.0
39	166908.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.48976183574665	23.200765444142178	10.905644484558872	24.403828235552304
2	15.553931144712807	23.603168763953725	39.24576948447331	21.59713060686016
3	16.125981794934244	27.682843450641336	32.65907868566325	23.532096068761163
4	11.553635525586007	18.0895807508222	43.62529226177395	26.731491461817843
5	11.484583266471356	38.61791376675963	38.35250607149041	11.544996895278603
6	26.089077897983277	42.99841355792923	19.356907617331405	11.555600926756087
7	23.750219148559104	33.61654883105445	26.792872987677274	15.84035903270917
8	21.573256846543146	37.52052433229144	24.282326041323536	16.62389277984188
9	23.156595246066285	14.886731391585762	24.914655426038085	37.04201793630987
10-14	21.002694621315186	25.481688527901014	32.228140165963815	21.287476684819985
15-19	20.38486411779707	30.903769637352973	28.308128997004044	20.403237247845915
20-24	20.31710162875977	29.58338792635352	29.694643533685593	20.40486691120112
25-29	20.2823204617818	29.789339160875745	29.471967210879914	20.456373166462548
30-34	20.203325043487766	29.769676209056286	29.50481202898821	20.52218671846774
35-39	20.24671314300831	29.840161299026068	29.54179335657975	20.37133220138587
40-44	20.015210618490535	29.36001319530349	30.04396468645586	20.580811499750116
45-49	19.829568678866206	28.978353094139	30.43694189500058	20.755136331994215
50-54	19.25197776811287	28.764002011073586	30.8848996438391	21.09912057697445
55-59	19.049058413658834	28.71317125184011	30.937239075275162	21.30053125922589
60-64	18.858518403577282	28.265678309201558	30.94432226595086	21.9314810212703
65-69	19.251243742761574	28.05638000354744	30.2538496558182	22.438526597872794
70-74	19.40031084686619	27.763255891352244	29.971970765072925	22.864462496708633
75-79	19.914096484577197	27.845720401025957	29.553993782343728	22.686189332053118
80-84	20.410250563322638	27.57757758392515	29.439240483693354	22.57293136905886
85-89	20.902995898511154	27.339874942034392	29.47885581390689	22.278273345547568
90-94	21.43722029609972	26.83383824692732	29.626065906680555	22.102875550292406
95-99	22.37503834793969	26.105172086022655	29.29686841117717	22.22292115486049
100-101	23.138230036281172	25.473797646281497	29.276876512607103	22.11109580483023
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.89359007129896
2	37.15106175157297
3	39.658077863695404
4	38.28512698740384
5	23.02958016174996
6	37.644678824739366
7	39.59057818126828
8	38.19714962638503
9	60.19861318237616
10-14	42.29017130613517
15-19	40.78810136564298
20-24	40.72196853996088
25-29	40.73869362824435
30-34	40.72551176195551
35-39	40.61804534439418
40-44	40.59602211824065
45-49	40.584705010860425
50-54	40.351098345087316
55-59	40.349589672884726
60-64	40.789999424847586
65-69	41.68977034063437
70-74	42.264773343574824
75-79	42.60028581663031
80-84	42.9831819323815
85-89	43.18126924405872
90-94	43.54009584639213
95-99	44.59795950280018
100-101	45.2493258411114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.0
2	6.0
3	10.0
4	16.5
5	19.0
6	32.5
7	48.5
8	65.5
9	85.5
10	98.0
11	136.5
12	201.0
13	284.5
14	401.5
15	591.0
16	875.5
17	1268.0
18	1875.5
19	2792.0
20	4048.5
21	5951.0
22	8692.0
23	12132.5
24	16272.0
25	21413.5
26	27310.0
27	33748.5
28	40981.0
29	49176.0
30	58315.0
31	67626.0
32	76975.5
33	87001.5
34	98309.0
35	110323.0
36	123342.5
37	135039.5
38	143842.0
39	150108.5
40	152115.0
41	151192.5
42	148443.5
43	144052.5
44	138546.5
45	130599.0
46	120567.5
47	109276.5
48	97248.5
49	84847.5
50	73500.5
51	64851.0
52	57145.0
53	50303.5
54	44337.5
55	39132.0
56	34907.0
57	30473.5
58	26224.5
59	23032.0
60	20174.0
61	17684.0
62	15566.5
63	13163.0
64	11145.0
65	10111.0
66	9173.0
67	8267.5
68	7461.5
69	6720.0
70	6165.5
71	5498.0
72	4766.0
73	3977.0
74	3285.5
75	2659.0
76	2040.0
77	1540.0
78	1185.5
79	904.5
80	657.0
81	484.5
82	352.0
83	250.0
84	187.5
85	139.0
86	107.5
87	86.0
88	63.5
89	46.5
90	33.0
91	30.0
92	33.0
93	25.5
94	18.0
95	17.5
96	19.0
97	17.5
98	12.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03439763090676276
2	0.03192480582775125
3	0.02786683646732209
4	0.0273912931828968
5	0.0324954577690616
6	0.001870470252072814
7	0.0010778981113639945
8	0.003297100105348689
9	4.755432844252917E-4
10-14	0.007164852152007728
15-19	0.024373178471077617
20-24	0.02054981046429827
25-29	0.0022192019939846945
30-34	0.002111412182848295
35-39	0.0061757221204031204
40-44	0.013873182750967175
45-49	0.008426627000016168
50-54	0.004780795152755599
55-59	0.003823368006779345
60-64	0.010791662267891286
65-69	0.014570646234790936
70-74	0.01838133308731894
75-79	0.007507243316793937
80-84	0.010201988595203925
85-89	0.008293474880377086
90-94	0.008940213747195483
95-99	0.008604163159534944
100-101	0.005437044885262502
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3154287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.172460430602772
#Duplication Level	Relative count
1	100.0
2	7.6081843838193794
3	2.4254839827697183
4	1.603579739565282
5	1.2885577065900877
6	1.0973164331336338
7	0.9289746001881467
8	0.7773431697776898
9	0.7030747140664455
10++	7.348244788830025
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	943910	5.447707	13.233231	80-84
GACGC	894665	5.432268	13.63038	75-79
GTCTC	1344155	5.2795544	9.205545	60-64
CGCTG	899825	5.193274	13.259406	75-79
CTGTC	1310650	5.147954	9.105021	55-59
GCCGA	818000	4.96677	13.830164	80-84
CGACG	788360	4.786801	13.6593685	85-89
CTGCC	860130	4.707976	12.487071	80-84
TGCCG	813670	4.696037	13.120686	80-84
CCGAC	772270	4.4471006	12.885102	80-84
TCTCT	1646710	4.401795	7.2076716	60-64
CTCTT	1554025	4.1540403	6.987018	60-64
TGACG	947080	4.1265326	9.849523	75-79
TGTCT	1463440	4.1247787	6.9651	55-59
ACACA	1304700	4.06103	7.605423	65-69
CTGAC	977260	4.038273	9.235772	75-79
ATCTG	1260990	3.7391684	7.697618	70-74
GACGA	813955	3.7310975	10.635757	85-89
ACGCT	883145	3.649367	9.394097	75-79
TACAC	1229360	3.6371984	7.020193	65-69
CACAT	1225690	3.6263404	6.9861	70-74
ACATC	1199625	3.5492241	7.129312	70-74
CATCT	1251270	3.5188549	6.983625	70-74
TCTGA	1159590	3.4384902	7.1346993	70-74
ATACA	1373170	3.067098	5.4978933	65-69
TCTTA	1493120	3.0131645	5.1158686	60-64
TGCAG	677960	2.9539468	9.8970175	90-94
TATAC	1307645	2.776231	5.640547	5
GCAGT	562130	2.4492626	9.377611	90-94
ACGAT	769760	2.4013546	7.151703	85-89
ATGCA	765530	2.3881586	7.23315	90-94
CAGTG	543135	2.3664994	9.263006	95-97
GTGTG	535750	2.3395708	9.496339	95-97
AGAAG	656980	2.273546	5.1971498	5
CGATA	701915	2.1897044	7.0212865	85-89
ATATG	973155	2.1785161	5.639059	90-94
TATGC	683735	2.0274546	6.701836	90-94
GATAT	874835	1.9584159	5.421449	85-89
GGTGG	301890	1.9371264	9.171455	95-97
TGTGT	650095	1.9320377	6.2619667	95-97
AGTGT	612855	1.9161702	6.700643	95-97
AGAGA	528355	1.8284262	5.380481	8
GTGTA	541265	1.6923348	7.4273925	1
CTCGG	289505	1.6708567	9.0460415	95-97
GAGAG	345145	1.6682104	6.0637307	7
AGATC	524845	1.6373142	5.3364854	95-97
GATCT	536245	1.590108	5.2292104	95-97
TGTAG	506445	1.5834658	5.853965	95-97
CGCCG	195685	1.5738486	5.138661	95-97
CTTTG	532260	1.5002013	6.3409047	9
GTAGA	454870	1.4962401	5.813548	95-97
CGGTG	240660	1.4645362	8.585959	95-97
TCTCG	350480	1.3766108	6.2065163	95-97
AAGAC	351655	1.1541317	5.500464	5
TCGGT	252595	1.0461302	5.8392205	95-97
GTCTT	351705	0.9912981	5.370653	1
GTGTT	329255	0.97852325	5.1741867	1
TGGAC	207815	0.905473	5.7213635	5
GTCCA	204825	0.8463861	5.953494	1
GGACT	182705	0.79606587	5.030904	6
GTATA	295910	0.66242766	6.9218273	1
>>END_MODULE