##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-174_CGAGGCTG-ACTGCATA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3154287	
Filtered Sequences	0	
Sequence length	101	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37505211161825	33.0	31.0	34.0	31.0	34.0
2	32.56892762136103	34.0	31.0	34.0	31.0	34.0
3	32.67987789316572	34.0	31.0	34.0	31.0	34.0
4	36.222406521664006	37.0	35.0	37.0	35.0	37.0
5	35.930185173384665	37.0	35.0	37.0	35.0	37.0
6	36.012394243136406	37.0	35.0	37.0	35.0	37.0
7	36.01420733116549	37.0	35.0	37.0	35.0	37.0
8	36.03740718583946	37.0	35.0	37.0	35.0	37.0
9	37.91207363185404	39.0	38.0	39.0	35.0	39.0
10-14	38.08894815214976	39.2	38.2	39.4	35.2	39.4
15-19	39.190332014810316	40.0	39.0	41.0	36.0	41.0
20-24	39.10914916746638	40.0	39.0	41.0	36.0	41.0
25-29	38.88970845075289	40.0	38.2	41.0	35.2	41.0
30-34	38.65505199748786	40.0	38.0	41.0	34.6	41.0
35-39	38.38035017105292	40.0	38.0	41.0	34.0	41.0
40-44	38.0853488601386	40.0	37.8	41.0	33.0	41.0
45-49	37.87253043239248	40.0	37.0	41.0	33.0	41.0
50-54	37.81599537391493	40.0	36.8	41.0	33.0	41.0
55-59	37.42050048077426	39.2	35.8	41.0	32.4	41.0
60-64	36.85396135481648	38.6	35.0	40.4	31.4	41.0
65-69	36.11090550733017	37.4	35.0	40.0	30.8	41.0
70-74	35.54204655441943	36.2	34.6	39.0	30.6	40.8
75-79	34.32616233082151	35.0	33.4	37.4	29.4	39.2
80-84	34.131939484263796	35.0	34.0	36.2	30.0	38.0
85-89	33.49633150058951	35.0	33.8	35.2	29.0	36.6
90-94	33.06839003552942	35.0	33.0	35.0	29.0	36.0
95-99	32.76968506670446	35.0	33.0	35.0	27.8	35.2
100-101	32.37095784245378	34.5	32.5	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	5.0
13	22.0
14	70.0
15	211.0
16	435.0
17	837.0
18	1378.0
19	2278.0
20	3240.0
21	4736.0
22	6554.0
23	8844.0
24	11608.0
25	15054.0
26	18984.0
27	24465.0
28	31084.0
29	38765.0
30	48090.0
31	60609.0
32	77738.0
33	103931.0
34	150539.0
35	239557.0
36	419061.0
37	756508.0
38	934485.0
39	195045.0
40	152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.067614033730614	23.23857126462217	11.208812390506125	24.485002311141095
2	15.78118990599931	23.46573922616128	39.07384255684922	21.67922831099019
3	16.225822190561605	27.46503409486835	32.839941324299275	23.469202390270766
4	11.714945406045803	18.057931951024113	43.47239170056498	26.754730942365107
5	11.334415669848685	38.82053218365989	38.357289618858395	11.487762527633027
6	26.104821786983873	43.18221518840866	19.202564636635792	11.510398387971671
7	23.687191431851318	33.65302523200964	26.810971861469802	15.848811474669237
8	21.57175298252822	37.65297831173891	24.17620844266866	16.599060263064207
9	23.111054891327264	14.725673345513583	24.8597860625872	37.303485700571954
10-14	20.942387297034163	25.50807837080139	32.24566439261868	21.303869939545766
15-19	20.32884123764889	30.985175691121153	28.27466268068153	20.411320390548426
20-24	20.28216638377999	29.642858977107387	29.674657177043944	20.400317462068678
25-29	20.274416306336885	29.810719690570448	29.439634551275905	20.475229451816766
30-34	20.191891201413476	29.79275417981384	29.467653496569767	20.54770112220292
35-39	20.211146405569288	29.86752875384091	29.51595637018878	20.40536847040102
40-44	20.005971778493834	29.386288431192092	29.986657065132853	20.621082725181214
45-49	19.81559912522341	28.992138746291	30.403307530912947	20.788954597572637
50-54	19.200418825636362	28.765183729743026	30.857783119317222	21.17661432530339
55-59	18.917564445472845	28.64735167303751	30.838378502631304	21.596705378858346
60-64	18.52617456050622	28.238945056339254	30.69164149043303	22.543238892721494
65-69	18.72983943735106	28.277007819664306	29.68472119788907	23.308431545095562
70-74	18.869298804938513	28.13365042359018	28.879688824432638	24.117361947038674
75-79	19.600769071618608	28.12440100555296	27.9672096450605	24.30762027776793
80-84	19.949020398054554	27.828856856516154	27.447172433453304	24.774950311975992
85-89	20.2602910171053	27.405111533138466	27.480078420068576	24.854519029687662
90-94	20.47295000067846	26.68410113870639	27.595707934295323	25.247240926319826
95-99	20.627270526472373	26.005404054321563	27.820519047408293	25.54680637179777
100-101	20.615572052688318	25.174100442081098	28.445570320124798	25.764757185105786
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.55261634487171
2	37.4604182169895
3	39.695024580832374
4	38.46967634841091
5	22.822178197481712
6	37.615220174955546
7	39.53600290652056
8	38.17081324559243
9	60.41454059189921
10-14	42.24625723657993
15-19	40.74016162819732
20-24	40.682483845848665
25-29	40.749645758153655
30-34	40.73959232361639
35-39	40.61651487597031
40-44	40.62705450367505
45-49	40.60455372279605
50-54	40.37703315093975
55-59	40.51426982433119
60-64	41.06941345322771
65-69	42.038270982446626
70-74	42.98666075197718
75-79	43.90838934938654
80-84	44.72397071003055
85-89	45.11481004679296
90-94	45.720190926998285
95-99	46.174076898270144
100-101	46.38032923779411
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	3.0
6	6.0
7	5.5
8	10.0
9	23.0
10	43.5
11	70.5
12	110.5
13	213.5
14	328.5
15	488.5
16	761.0
17	1142.0
18	1738.0
19	2628.5
20	3952.0
21	5939.0
22	8617.5
23	12170.0
24	16276.0
25	21030.0
26	26907.0
27	33478.5
28	40862.5
29	48741.5
30	57070.5
31	65954.0
32	74922.5
33	84429.0
34	95021.5
35	105636.0
36	116796.5
37	127399.5
38	135244.0
39	139633.5
40	140538.0
41	138959.5
42	135342.5
43	131257.5
44	128351.0
45	124128.0
46	119138.0
47	113801.0
48	105925.5
49	96135.0
50	85801.0
51	77237.5
52	69083.5
53	60701.5
54	53243.5
55	46463.0
56	40814.5
57	35595.0
58	30690.5
59	26759.0
60	22938.0
61	19813.0
62	17364.0
63	14870.0
64	12800.5
65	11368.5
66	10211.0
67	8953.5
68	7752.0
69	7048.0
70	6481.0
71	5667.0
72	4740.5
73	3918.5
74	3281.0
75	2683.5
76	2026.0
77	1452.0
78	1034.0
79	733.0
80	516.0
81	354.5
82	243.0
83	157.0
84	93.5
85	54.0
86	32.5
87	29.0
88	22.5
89	8.0
90	3.5
91	5.5
92	5.0
93	3.5
94	3.0
95	2.5
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.1702885628352774E-5
2	0.01185687922500394
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011222821512436884
20-24	6.467388668183967E-4
25-29	0.016149449939082905
30-34	0.005934780189627641
35-39	0.03789128890300724
40-44	0.04620378551476134
45-49	0.04698367650121882
50-54	0.04664762591355828
55-59	0.03714944137930379
60-64	0.0479347630700694
65-69	0.0408079543808157
70-74	0.0033668464537310655
75-79	0.0013442023506421577
80-84	1.8387673664444612E-4
85-89	0.002992752403316502
90-94	0.002415759884880482
95-99	0.0019846006403348836
100-101	4.755432844252917E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3154287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.541179476183743
#Duplication Level	Relative count
1	100.0
2	7.711416523310624
3	2.4208022556064117
4	1.635190440998379
5	1.301072530912117
6	1.101098614466436
7	0.947081436582806
8	0.8216319610485588
9	0.7092242626738128
10++	7.5598834934573755
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	1457460	8.43904	24.970184	90-94
CGAGC	919970	5.326845	13.2521305	75-79
GAGCC	919185	5.3223	13.187557	75-79
GTCTC	1347430	5.163693	8.999582	60-64
CTGTC	1316050	5.043437	8.911181	55-59
CTCCG	988845	4.9748063	11.272796	75-79
AGCCC	877190	4.547751	11.565592	80-84
GCCCA	866175	4.490644	11.763178	80-84
TCTCT	1654680	4.324949	7.0883174	60-64
TGTCT	1465220	4.2772365	7.1998	55-59
CGAGG	642615	4.155667	14.704657	90-94
ATCTC	1539795	4.1474853	11.260763	95-97
CCACG	790310	4.0973253	11.684243	80-84
GGCTG	649420	4.075308	14.762626	95-97
CTCTT	1555210	4.0649576	6.8515916	60-64
TCTCC	1171875	4.021074	8.323068	70-74
GAGGC	617120	3.9907954	14.787439	90-94
TCCGA	1003710	3.963852	9.121266	75-79
CCCAC	833535	3.8693082	10.570307	80-84
GACCG	659820	3.820515	12.959202	85-89
ACACA	1312375	3.7539628	7.0148892	70-74
CGAGA	823765	3.7442172	10.595063	85-89
GAGAC	812050	3.6909692	10.710111	85-89
ACGAG	800745	3.6395853	10.274601	85-89
TACAC	1238280	3.437129	6.6006413	65-69
CACAT	1234205	3.425818	6.536895	70-74
CATCT	1257150	3.3861725	6.72025	70-74
ACATC	1207810	3.3525527	6.7167106	70-74
CACGA	795150	3.2360353	9.239835	80-84
TCTTA	1495425	3.0682647	5.221449	60-64
AGACC	745010	3.03198	9.416336	85-89
ATACA	1380350	3.007651	5.3560123	65-69
CTTAT	1416265	2.9058468	5.080038	60-64
AGGCT	648010	2.8581457	10.253368	90-94
TATAC	1313030	2.7762454	5.7184396	5
ACCGA	670275	2.7278295	9.055471	90-94
GCTGA	596580	2.6313057	10.099435	95-97
AGAAG	654840	2.336437	5.5029855	5
GATCT	675740	2.0328019	6.9252186	95-97
TGATC	673745	2.0268006	7.09102	95-97
CTGAT	636765	1.9155549	7.084212	95-97
TCTCG	488675	1.8727262	7.6917014	95-97
AGAGA	523390	1.8674299	5.3524795	8
GAGAG	341100	1.731541	6.2172775	7
CTCGT	426445	1.6342452	7.392081	90-94
AAGAG	446110	1.5916986	5.0410876	5
CTTTG	532435	1.554272	6.605627	9
GTATG	443350	1.4895517	6.7944164	95-97
TGCCG	258280	1.4512163	10.646253	95-97
GCCGT	243265	1.3668505	9.926088	95-97
ATGCC	339125	1.3392726	7.7164245	95-97
GTCTT	421715	1.2310607	5.3637557	1
CCGTC	238580	1.2002784	8.022425	95-97
TCGTA	390740	1.1754477	5.926525	95-97
TATGC	379725	1.1423116	5.950835	95-97
AAGAC	353500	1.1293145	5.1843824	5
CGTAT	355555	1.069602	5.9357624	95-97
GTGTT	325255	1.0604198	5.709069	1
TGGAC	206455	0.91060084	5.8916926	5
CGTCT	237320	0.90947026	5.494692	95-97
GTGTA	261135	0.8773522	8.231069	1
GTCCA	207760	0.82048583	5.6399913	1
GGACT	182220	0.8037087	5.143201	6
GTATA	293820	0.6938379	7.150288	1
>>END_MODULE