##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-173_CGAGGCTG-GTAAGGAG_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2438877	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.541249927733134	31.0	31.0	34.0	30.0	34.0
2	31.74501051098518	33.0	31.0	34.0	30.0	34.0
3	31.886169331212685	34.0	31.0	34.0	30.0	34.0
4	35.4295046449657	37.0	35.0	37.0	33.0	37.0
5	35.28488275546491	37.0	35.0	37.0	32.0	37.0
6	35.422064745372566	37.0	35.0	37.0	33.0	37.0
7	35.43899015817526	37.0	35.0	37.0	33.0	37.0
8	35.48594332555516	37.0	35.0	37.0	33.0	37.0
9	37.28325003679972	39.0	37.0	39.0	34.0	39.0
10-14	37.39405808493008	39.2	37.2	39.4	33.2	39.4
15-19	38.406618537958245	40.0	38.0	41.0	33.6	41.0
20-24	38.29717087003568	40.0	38.0	41.0	33.6	41.0
25-29	38.09485619816005	40.0	38.0	41.0	33.0	41.0
30-34	37.810910103297545	40.0	38.0	41.0	32.2	41.0
35-39	37.46768664430391	40.0	37.0	41.0	31.2	41.0
40-44	37.40481590502513	40.0	37.0	41.0	31.0	41.0
45-49	37.228703702564744	40.0	36.6	41.0	31.0	41.0
50-54	36.51224444693192	38.6	35.4	40.2	30.2	40.6
55-59	37.14250788375141	39.6	35.8	41.0	31.0	41.0
60-64	36.67780933601817	39.0	35.0	41.0	30.6	41.0
65-69	35.99682197995225	37.6	35.0	40.0	29.4	41.0
70-74	35.13902775744738	36.4	34.0	39.2	29.0	40.8
75-79	34.14969373199223	35.2	33.8	37.6	27.6	39.4
80-84	33.19578084503646	35.0	33.0	36.2	26.2	38.0
85-89	32.36816141199413	35.0	32.4	35.2	25.2	36.6
90-94	31.70160766615126	35.0	31.6	35.0	23.6	36.0
95-99	31.077530191149453	34.0	31.0	35.0	20.2	35.0
100-101	30.073688833016178	34.0	29.0	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	5.0
6	70.0
7	262.0
8	574.0
9	765.0
10	956.0
11	1316.0
12	1977.0
13	2761.0
14	3417.0
15	4208.0
16	4824.0
17	5669.0
18	6548.0
19	7418.0
20	8563.0
21	9778.0
22	11287.0
23	12919.0
24	14991.0
25	17477.0
26	20313.0
27	24628.0
28	29023.0
29	35034.0
30	43050.0
31	53913.0
32	69557.0
33	92378.0
34	130235.0
35	195803.0
36	324315.0
37	557798.0
38	626138.0
39	120829.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63360094894576	23.36171912747521	10.612311193179535	24.39236873039949
2	15.199584601206084	23.723380824473093	39.67735227564958	21.39968229867124
3	15.820128019463356	28.187167457611544	32.63774478380361	23.354959739121483
4	11.25247411458893	18.423653724849746	44.13160115919395	26.192271001367377
5	11.08670686190066	39.126491940445426	38.612444116320084	11.174357081333826
6	25.51048231912059	43.74228366903516	19.536202123895112	11.211031887949135
7	23.598600168521013	33.87879197905583	27.009654080894187	15.51295377152897
8	21.36501278289982	37.91000100459036	24.4548867170602	16.270099495449617
9	23.05388583190507	14.783997441427552	25.148224990364348	37.01389173630303
10-14	20.633755949381207	25.80157698838602	32.70578287849382	20.85888418373895
15-19	19.994381245993562	31.285968740861836	28.60241081458399	20.117239198560615
20-24	19.976041263817184	29.909484206386345	30.03725046229862	20.077224067497852
25-29	19.94594440776463	30.247103663874526	29.688153619701573	20.11879830865927
30-34	19.818676899337913	30.129199720784012	29.762326900518804	20.289796479359275
35-39	19.937160539469357	30.299684785771436	29.730591722944766	20.03256295181444
40-44	19.65364554755675	29.918400046454202	30.208681391270513	20.219273014718535
45-49	19.659266004905636	29.650110175043327	30.357587479837328	20.333036340213713
50-54	19.248644639523484	29.351447669652554	30.77613843883336	20.623769251990602
55-59	19.281890302063253	29.327205571505694	30.809126495429723	20.58177763100133
60-64	19.07431900835488	28.888670548514305	30.91230035353918	21.124710089591638
65-69	19.4562720755678	28.847401618782705	30.263371032754165	21.432955272895335
70-74	19.38858334359959	28.433898625382483	30.12926624456633	22.04825178645159
75-79	19.50815236802116	28.46111408492969	29.793493244857423	22.237240302191726
80-84	19.449995882895777	28.205551103105087	29.731258072230737	22.6131949417684
85-89	19.669798241383223	28.03053931193383	29.652072981193907	22.64758946548905
90-94	19.909300273160426	27.505566421811633	29.931541356531397	22.653591948496544
95-99	20.447859217064853	27.125504047470017	29.54316863955406	22.883468095911063
100-101	20.915578605690968	26.312684982667296	29.88284533583298	22.888891075808758
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.02596967934525
2	36.59926689987733
3	39.17508775858484
4	37.44474511595631
5	22.261063943234486
6	36.72151420706973
7	39.11155394004998
8	37.63511227834944
9	60.0677775682081
10-14	41.49264013312016
15-19	40.11162044455418
20-24	40.05326533131503
25-29	40.0647427164239
30-34	40.108473378697184
35-39	39.9697234912838
40-44	39.87291856227528
45-49	39.99230234511935
50-54	39.872413891514086
55-59	39.86366793306458
60-64	40.199029097946514
65-69	40.88922734846313
70-74	41.43683513005118
75-79	41.74539267021289
80-84	42.063190824664176
85-89	42.317387706872275
90-94	42.56289222165697
95-99	43.331327312975915
100-101	43.804469681499725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	7.0
2	9.0
3	15.0
4	19.5
5	27.0
6	37.5
7	44.5
8	52.5
9	64.5
10	84.0
11	109.5
12	156.5
13	225.0
14	329.5
15	478.0
16	716.0
17	1052.5
18	1625.0
19	2385.5
20	3483.0
21	5135.5
22	7311.5
23	10153.5
24	13553.0
25	17704.0
26	22769.0
27	28214.5
28	34205.0
29	40986.0
30	48319.5
31	56000.0
32	63802.5
33	72675.0
34	82602.0
35	93459.5
36	106305.5
37	117427.5
38	124293.5
39	126149.0
40	123272.5
41	118295.0
42	111367.0
43	104678.5
44	99195.0
45	92540.5
46	85390.5
47	77672.5
48	69122.0
49	60456.0
50	52198.0
51	45829.0
52	40736.0
53	36112.5
54	31983.0
55	28381.5
56	25218.5
57	22138.0
58	19135.5
59	16616.5
60	14261.0
61	12363.5
62	10786.5
63	8845.5
64	7281.5
65	6364.0
66	5558.5
67	4759.0
68	4164.5
69	3875.0
70	3483.5
71	2965.5
72	2507.5
73	2011.5
74	1625.0
75	1337.5
76	1028.5
77	798.0
78	636.5
79	451.0
80	327.5
81	260.0
82	193.5
83	138.0
84	95.0
85	81.5
86	65.0
87	47.5
88	39.5
89	32.0
90	28.0
91	25.0
92	19.5
93	20.5
94	21.0
95	17.5
96	12.0
97	8.0
98	6.5
99	5.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03292498965712498
2	0.030259828601442385
3	0.026815620467944876
4	0.025872563479011037
5	0.03185892523485194
6	0.0022961387556650046
7	4.920297333567867E-4
8	0.0029521784001407207
9	6.970421222554479E-4
10-14	0.007298441044792336
15-19	0.02517552135675559
20-24	0.021239283489901294
25-29	0.0022305347912174333
30-34	0.0019763194289830937
35-39	0.0063389830647466025
40-44	0.014195057807343299
45-49	0.008700725784859179
50-54	0.004764487918004885
55-59	0.003952638857966187
60-64	0.011431490804989347
65-69	0.015498936600738783
70-74	0.019180959105358737
75-79	0.0076592628492539805
80-84	0.011062468504971756
85-89	0.00862692132485566
90-94	0.009709386738240592
95-99	0.00893033966042568
100-101	0.005535334500263851
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2438877.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.126434220786457
#Duplication Level	Relative count
1	100.0
2	7.545675162519332
3	2.9115165537099674
4	2.0263048699216637
5	1.6094757887060067
6	1.337876749361868
7	1.1216034402544983
8	0.9826604131826127
9	0.817311923952269
10++	7.387871091048548
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	531550	4.237816	9.568855	90-94
GACGC	474545	3.930563	9.602187	85-89
GTCTC	745590	3.9270155	6.5098886	60-64
CGCTG	479765	3.8249574	9.262894	75-79
CTGTC	714675	3.7641866	6.257766	65-69
GCCGA	439560	3.640789	9.656538	80-84
CTGCC	474850	3.554038	8.95462	80-84
CGACG	424485	3.5159252	9.741897	95-97
TCTCT	992405	3.4531548	5.311902	60-64
TGCCG	431500	3.4401615	9.242522	90-94
CTCTT	928300	3.230096	4.985724	70-74
CCGAC	414155	3.2203858	8.871525	95-97
TGTCT	841880	3.1203961	4.850035	60-64
ACACA	789460	3.0803158	6.3934817	6
TGACG	510400	2.9749863	6.852186	85-89
CTGAC	533305	2.9182167	6.345707	85-89
GACGA	455885	2.7606342	7.3239684	95-97
ATCTG	713830	2.7487397	5.2432957	70-74
TACAC	728880	2.7374222	7.471295	5
ACGCT	472975	2.5880942	6.2767096	85-89
GACTC	472530	2.585659	6.9900436	85-89
AGAAG	577255	2.5556276	5.3904366	5
CTCCT	466555	2.3069227	6.002713	90-94
ACTCC	436805	2.243867	5.820745	90-94
ACGAC	386400	2.1966374	6.4829097	85-89
CGACT	395740	2.1654682	6.0670605	85-89
TATAC	761125	2.0115862	5.552534	5
CAAAG	483765	2.0106292	5.090721	4
GGTGG	208235	1.8837203	6.7538257	95-97
AGAGA	411570	1.8221056	5.062675	8
CTTTG	483570	1.7923335	7.75611	9
GAGAG	265780	1.7143847	5.8777018	7
GTGTA	369245	1.5145577	8.900247	1
ACGTG	252835	1.4737083	6.001762	95-97
CTCGG	180570	1.439606	6.5026803	95-97
CGTGT	242015	1.3578044	5.607343	95-97
CGGTG	151025	1.2825644	6.0493402	95-97
AAGAC	304570	1.265857	5.6814456	5
GAGTC	207355	1.2086172	6.1452208	9
TATGA	417745	1.1760516	5.528101	4
GATTG	277815	1.1395328	5.4940124	7
GTCTT	302460	1.1210563	6.0071416	1
GTGTT	282225	1.114262	5.7993546	1
TCATA	419295	1.1081598	5.320506	2
GACTT	285900	1.1009129	5.3351164	7
TGGAC	174055	1.0145204	6.6162286	5
GTCCA	172510	0.9439657	6.811308	1
GGACT	156450	0.9119055	5.921645	6
GTATA	248560	0.69975555	7.0892143	1
>>END_MODULE